Potential environmental fate of elsinochrome A,a perylenequinone toxin produced in culture by bindweed biocontrol fungus Stagonospora convolvuli LA39

The photosensitizing perylenequinone toxin elsinochrome A (EA) is produced in culture by the bindweed biocontrol fungus Stagonospora convolvuli LA39 where it apparently plays a pathogenicity related role. We investigated the fate of EA with reference to its stability under different temperature and light conditions. EA remained stable when boiled in water at 100^∘C for 2 h. Similarly, exposing EA to 3–27^∘C in the dark for up to 16 weeks did not affect its stability either in dry or in aqueous form. However, results from irradiation experiments indicate that direct photolysis may be a significant degradation pathway for EA in the environment. EA either in dry form or dissolved in water was degraded by different irradiation wavelengths and intensities, with degradation plots fitting a first order rate kinetics. EA degraded faster if exposed in aqueous form, and at higher quantum flux density (μmol s⁻¹ m⁻²). Sunlight was more effective in degrading EA than artificial white light and ultraviolet radiations (UV-A or UV-B). Exposing EA to natural sunlight, particularly, during the intense sunshine (1,420– 1,640 μmol s⁻¹ m⁻²) days of 30 July to 5 August 2004 in Zurich caused the substance to degrade rapidly with half-life under such condition only 14 h. This implies that should EA gets into the environment, particularly on exposed environmental niches, such as on plant surfaces through biocontrol product spray, or released from shed diseased leaves, it may have no chance of accumulating to ‘level of concern’. Furthermore, a toxicity assay using Trichoderma atroviride P1 as biosensor showed that photo-degraded EA was not toxic, indicating that no stable toxic by-products were left.     

Trace Gas Emissions from Biomass Burning from Northeast Region in India—Estimates from Satellite Remote Sensing Data and GIS

Biomass burning associated with shifting cultivation areas from the northeastern region of India is an important source of trace gas emissions in the Southeast Asian region. In the present study, satellite data pertaining to IRS-P4 OCM data and DMSP-OLS has been used to quantify the intensity, areal extent and amount of biomass burnt in the northeastern region states at district level. Trace gas emissions have been quantified both by using IPCC based emission ratios and ground based emission ratios obtained from field based studies. Areal estimates with respect to shifting cultivation areas from IRS-P4 OCM satellite data of 4th April 2000 suggested nearly 112.99 km² of the northeastern region of India affected due to shifting cultivation. In the study, DMSP OLS nighttime data has been used to capture the real time fires during the dry season. The results suggested high amount of fires during the March season when compared to April and May. Using the emission ratios obtained from the ground-based studies and IPCC emission ratios, the emissions for the individual non-CO₂ trace gases have been computed in a GIS framework using the biomass data, combustion factors and emission ratios. Results suggested emissions of 2.063 Mt CH₄, 17.94 Mt CO, 1.419 Mt N₂O, and 51.28 Mt NO _x and 2.643 Mt release of CH₄, 3.7204 Mt CO, 0.145 Mt N₂O, and 8.477 Mt NO _x , respectively, from biomass burning due to shifting cultivation for the year 2000, from the northeastern region in India. The study highlights the importance of Satellite Remote sensing data and GIS in quantifying the trace gas emissions from biomass burning.     

Periodical monitoring with lichen, <Emphasis Type="Italic">Phaeophyscia hispidula</Emphasis> (Ach.) Moberg in Dehradun city,Uttarakhand, India

Nine heavy metals were estimated in lichen, Phaeophyscia hispidula (Ach.) Moberg, collected from 12 different sites of Dehradun, capital city, to analyze the air quality of Uttarakhand. Total metal concentration was the highest at Mohkampur Railway Crossing, Hardwar Road (42,505 μg g⁻¹). Dela Ram Chowk, located in the center of the city, also had higher metal concentration, 34,317 μg g⁻¹, with maximum concentration of Pb at 12,433 μg g⁻¹, while Nalapani forest area had minimum total metal concentration (1,873 μg g⁻¹) as well as minimum Pb level at 66.6 μg g⁻¹, indicating anthropogenic activity, mainly vehicular activity, responsible for the increase in metal concentration in the ambient environment. In comparison with the earlier years 2004 and 2006, air pollution as indicated by similar lichen shows a considerable increase in the total metal concentration (especially Pb) in the ambient air of Dehradun city, which may be attributed to exponential rise in the traffic activity in the last 5 years.     

Evaluation of tidal marsh restoration: Comparison of selected macroinvertebrate populations on a restored impounded valley marsh and an unimpounded valley marsh within the same salt marsh system in Connecticut,USA

Macroinvertebrates were examined on an impounded valley marsh in Stonington, Connecticut, that has changed from aTypha-dominated system to one with typical salt-marsh vegetation during 13 years following the reintroduction of tidal exchange. Animal populations on this restored impounded marsh were evaluated by comparing them with populations on a nearby unimpounded valley marsh of roughly the same size. Populations of the high marsh snail,Melampus bidentatus Say, were quantitatively sampled along transects that extended from the water-marsh edge to the upland; those of the ribbed mussel,Geukensia demissa Dillwyn, were sampled in low marsh areas on transects along the banks of creeks and mosquito ditches. The occurrence of other marsh invertebrates also was documented, but their abundance was not measured. The mean density ofMelampus was 332±39.6 SE/m² on the restored impounded marsh and 712±56.0 SE/m² on the unimpounded marsh. However, since snails were larger on the restored impounded marsh, the difference in snail biomass was less pronounced than the difference in snail density. MeanMelampus biomass was 4.96±0.52 SE g dry wt/m² on the restored impounded marsh and 6.96±0.52 SE g dry wt/m² on the unimpounded marsh. On the two marshes, snail density and biomass varied in relation to plant cover and other factors. The density and biomass ofGeukensia at the edge of the marsh were comparable on the restored impounded and unimpounded marshes. Mean mussel densities ranged from 80 to 240/m² and mean mussel biomass varied from 24.8–64.8 g dry wt/m² in different low marsh areas. In contrast, below the impoundment dike, meanGeukensia density was 1100±96.4 SE/m² and meanGeukensia biomass was 303.6±33.28 SE g dry wt/m². A consideration of all available evidence leads to the conclusion that the impounded marsh is in an advanced phase of restoration.     

Effectiveness of a Coral-Derived Surfacing Material for Reducing Sediment Production on Unpaved Roads, Schoffield Barracks, Oahu, Hawaii

This study evaluated the effectiveness of two application rates of a coral-derived surfacing material for both traffic and nontraffic road conditions using simulated rainfall (110–120 mm h⁻¹ for 30–90 min) on 0.75-m (wide) × 5.0-m (long) plots of similar slope (roughly 0.1 m m⁻¹). The coral is a locally available material that has been applied to unpaved roads surfaces on Schoffield Barracks, Oahu, Hawaii (USA), where this experiment was conducted. The simulations show that compared with a bare control plot, the coral-based surface application rates of 80 and 160 kg m⁻² (equivalent to only 10- and 20-mm thicknesses) reduced road sediment production by 75% and 95%, respectively, for nontraffic conditions. However, after two passes of the research vehicle during wet conditions, sediment production rates for the two coral treatments were not significantly different from those on the bare road plots. The overall effectiveness of the coral-derived surfacing material is unsatisfactory, primarily because the on-road surface thickness associated with the application rates tested was too small. These rates were selected to bracket those applied to training roads in the study area. Furthermore, the composition of the coral-based material does not facilitate the development of a sealed, erosion-resistant surface. When applied at the low rates tested, the coral material breaks down under normal traffic conditions, thereby losing its ability to counter shearing forces exerted by overland flow on long hillslopes where erosion measures are most needed. These simulations, combined with observations on roads in the study area, indicate that this material is not an appropriate road surfacing material for the site—at least for the low application rates examined. These results are preliminary; extended testing of higher applications rates at the hillslope scale under natural climate and traffic conditions is needed to better judge the effectiveness of this material over time.     

Primary Productivity and the Impacts of the Exotic Weed <Emphasis Type="Italic">Eupatorium Glandulosum</Emphasis> in a Montane Grassland of Garhwal Himalaya

Summary The biomass and productivity of a montane grassland of Garhwal Himalaya were estimated with the objectives to compare these values of the dominant exotic species, Eupatorium glandulosum HBK. (Asteraceae) with other species, and to compare the sites more dominated by this species with other study sites. The effect of dominance of this species on other species was undertaken because of its continuous spread in the grasslands of the Garhwal Himalaya causing replacement of some native grasses and economically important herbaceous plants. Out of six study sites, SW₁, SW₂, and NE₁ were more dominated by Eupatorium glandulosum. Total net primary productivity (TNP) ranged from 1528.5 to 2163.4 g m⁻² yr⁻¹. Eupatorium glandulosum showed individual highest biomass on all the study sites, and the sites more dominated by this species showed higher values of primary productivity, thereby reducing the biomass and production of other species on these sites.     

Physiological and Morphological Response Patterns of <Emphasis Type="Italic">Populus deltoides</Emphasis> to Alluvial Groundwater Pumping

We examined the physiological and morphological response patterns of plains cottonwood [Populus deltoides subsp. monilifera (Aiton) Eck.] to acute water stress imposed by groundwater pumping. Between 3 and 27 July 1996, four large pumps were used to withdraw alluvial groundwater from a cottonwood forest along the South Platte River, near Denver, Colorado, USA. The study was designed as a stand-level, split-plot experiment with factorial treatments including two soil types (a gravel soil and a loam topsoil over gravel), two water table drawdown depths (∼0.5 m and >1.0 m), and one water table control (no drawdown) per soil type. Measurements of water table depth, soil water potential (Ψ_s), predawn and midday shoot water potential (Ψ_pd and Ψ_md), and D/H (deuterium/hydrogen) ratios of different water sources were made in each of six 600-m² plots prior to, during, and immediately following pumping. Two additional plots were established and measured to examine the extent to which surface irrigation could be used to mitigate the effects of deep drawdown on P. deltoides for each soil type. Recovery of tree water status following pumping was evaluated by measuring stomatal conductance (g _s ) and xylem water potential (Ψ_xp) on approximately hourly time steps from before dawn to mid-afternoon on 11 August 1996 in watered and unwatered, deep-drawdown plots on gravel soils. P. deltoides responded to abrupt alluvial water table decline with decreased shoot water potential followed by leaf mortality. Ψ_pd and percent leaf loss were significantly related to the magnitude of water table declines. The onset and course of these responses were influenced by short-term variability in surface and ground water levels, acting in concert with physiological and morphological adjustments. Decreases in Ψ_pd corresponded with increases in Ψ_md, suggesting shoot water status improved in response to stomatal closure and crown dieback. Crown dieback caused by xylem cavitation likely occurred when Ψ_pd reached −0.4 to −0.8 MPa. The application of surface irrigation allowed trees to maintain favorable water status with little or no apparent cavitation, even in deep-drawdown plots. Two weeks after the partial canopy dieback and cessation of pumping, g _s and Ψ_xp measurements indicated that water stress persisted in unwatered P. deltoides in deep-drawdown plots.     

Spatial distribution of particulate matter (PM10) in Warri metropolis,Nigeria

This study examined the spatial distribution of particulate air pollution in the Warri metropolis. This was done to ascertain the differences between the distribution of particulate matter (PM10) in the urban area and the surrounding rural areas. To achieve this, the study generated data from field measurement of PM10 levels for the year 2003. Analysis of variance, the U-test, and simple regression statistical techniques were used to analyze the data. The major finding of the study was that the Warri metropolitan area is polluted with PM10 levels of over 126 μg/m³, which is 81% over the 70 μg/m³ threshold of the World Health Organization. However the built-up area of the Warri metropolis is 150% more polluted with PM10 particulates than the surrounding rural areas. While the traffic-clogged area of Enerhen, Jakpa, Deco, and Estate Junctions are the most polluted areas with levels of 151 μg/m³, traditional areas had the lowest levels of 128 μg/m³. The daily distribution of PM10 showed that Mondays were the most polluted days with levels of 145 μg/m³ and Fridays were the least polluted days with levels of 141.5 μg/m³ in the built-up area, whereas in the rural area Wednesdays were the most polluted days with levels of 57.1 μg/m³ and Sundays were the least polluted days with levels of 53.5 μg/m³. Mondays generally recorded the highest PM10 values because of the large amount of industrial operation, heavy vehicular traffic in the peak period, and increased commercial activities. The study also showed significant variation in the level of PM10 particulates within the urban areas of the Warri metropolis with a calculated F-value (3.29), which is greater than the critical F-value of 3.14 at the 0.05 significance level. It is therefore recommended that urban environmental management policy should be vigorously pursued to curb the adverse consequences of increased PM10 levels in urban areas of the Warri metropolis.     

Soil-erosion and runoff prevention by plant covers in a mountainous area (se spain): Implications for sustainable agriculture

In the Mediterranean region the intensities and amounts of soil loss and runoff on sloping land are governed by rainfall pattern and vegetation cover. Over a two-year period (1998–1999), six wild species of aromatic and mellipherous plants (Thymus serpylloides subsp. Gadorensis, Thymus baeticus Boiss, Salvia lavandulifolia Vahl., Santolina rosmarinifolia L., Lavandula stoechas L. and Genista umbellata Poiret) were selected for erosion plots to determine their effectiveness in reducing water erosion on hillslopes of the Sierra Nevada Mountain (SE Spain). The erosion plots (including a bare-soil plot as control), located at 1,345 m in altitude, were 2 m² (2 m × 1 m) in area and had 13% incline. The lowest runoff and soil erosion rates, ranging from 9 to 26 mm yr⁻¹ and from 0.01 to 0.31 Mg ha⁻¹ yr⁻¹, respectively, over the entire study period, were measured under the Thymus serpylloides. Lavandula stoechas L. registered the highest rates among the plant covers tested, runoff ranging from 77 to 127 mm yr⁻¹ and erosion from 1.67 to 3.50 Mg ha⁻¹ yr⁻¹. In the bare-soil plot, runoff ranged from 154 to 210 mm yr⁻¹ and erosion from 4.45 to 7.82 Mg ha⁻¹ yr⁻¹. According to the results, the lowest-growing plant covers (Thymus serpylloides and Salvia lavandulifolia Vahl.) discouraged the soil erosion and runoff more effectively than did the taller and open medium-sized shrubs (Santolina rosmarinifolia L., Genista umbellata Poiret, Thymus baeticus Boiss and Lavandula stoechas L.). Monitoring allowed more direct linkage to be made between plant covers and the prevention of erosion, with implications for sustainable mountain agriculture and environmental protection.     

Salt Marsh Diking and Restoration: Biogeochemical Implications of Altered Wetland Hydrology

Experimental short-term desalination and drainage of salt marsh cores in greenhouse microcosms caused Spartina production to increase after one growing season, reflecting decreased salt stress and sulfide toxicity. However, production thereafter declined, likely due to pyrite oxidation and acidification in drained treatments and sulfide accumulation in waterlogged treatments. A survey of longer-term (decadal) effects of diking on peat composition of Cape Cod, Massachusetts, USA, marshes revealed acidification, Fe(II) mobilization, and decreased organic content in drained sites. Despite the aerobic decomposition of organic matter, abundant nutrients remained as sorbed NH₄ and mineral-bound PO₄. In diked, seasonally waterlogged sites, porewater alkalinity, sulfide, ammonium and orthophosphate were much lower, and organic solids higher, than in adjacent natural marsh. Seawater was added to cores from diked marshes to study the effects of tidal restoration. Salination of the drained peat increased porewater pH, alkalinity, ammonium, orthophosphate, Fe, and Al; copious ammonium N, and Fe(II) for sulfide precipitation favored Spartina growth. Salination of diked–waterlogged peat increased sulfate reduction and caused 6–8 cm of sediment subsidence. The resulting increase in porewater sulfides and waterlogging decreased vigor of transplanted Spartina alterniflora. Results indicate that seawater restoration should proceed cautiously to avoid nutrient loading of surface waters in drained sites or sulfide toxicity in diked–waterlogged marshes.     

Response of Soil Inorganic Nitrogen to Land Use and Topographic Position in the Cofre de Perote Volcano (Mexico)

This study addressed the effects of land use and slope position on soil inorganic nitrogen and was conducted in small watersheds. The study covered three land use types: tropical cloud forest, grassland, and coffee crop. To conduct this research, typical slope small watersheds were chosen in each land use type. Slopes were divided into three positions: shoulder, backslope, and footslope. At the center of each slope position, soil sampling was carried out. Soil inorganic nitrogen was measured monthly during a period of 14 months (July 2005–August 2006) with 11 observations. Significant differences in soil NH₄ ⁺–N and NO₃ ⁻–N content were detected for both land use and sampling date effects, as well as for interactions. A significant slope position-by-sampling date interaction was found only in coffee crop for NO₃ ⁻–N content. In tropical cloud forest and grassland, high soil NH₄ ⁺–N and low NO₃ ⁻–N content were recorded, while soil NO₃ ⁻–N content was high in coffee crop. Low NO₃ ⁻–N contents could mean a substantial microbial assimilation of NO₃ ⁻–N, constituting an important mechanism for nitrogen retention. Across the entire land use set, the relationship between soil temperature and soil inorganic N concentration was described by an exponential decay function (N = 33 + 2459exp^−0.23T, R ² = 0.44, P < 0.0001). This study also showed that together, soil temperature and gravimetric soil water content explained more variation in soil inorganic N concentration than gravimetric soil water content alone.     

Production of vermifertilizer from guar gum industrial wastes by using composting earthworm <Emphasis Type="Italic">Perionyx sansibaricus</Emphasis> (Perrier)

Efforts have been made to convert the guar gum industrial waste into a value-added product, by employing a new earthworm species for vermicomposting e.g. Perionyx sansibaricus (Perrier) (Megascolecidae), under laboratory conditions. Industrial lignocellulosic waste was amended with other organic supplements (saw dust and cow dung); and three types of vermibeds were prepared: guar gum industrial waste + cow dung + saw dust in 40: 30: 30 ratio (T₁), guar gum industrial waste + cow dung + saw dust in 60: 20: 20 ratio (T_2,), and guar gum industrial waste + cow dung + saw dust in 75: 15: 10 ratio (T₃). As compared to initial concentrations, vermicomposts exhibited a decrease in organic C content (5.0–11.3%) and C:N ratio (11.1–24.4%) and an increase in total N (18.4–22.8%), available P (39.7–92.4%), and exchangeable K (9.4–19.7%) contents, after 150 days of vermicomposting. A vermicomposting coefficient (VC) was used to compare of vermicomposting with the experimental control (composting). P. sansibaricus exhibited maximum value of mean individual live weight (742.8 ± 21.1 mg), biomass gain (442.94 ± 21.8 mg), growth rate (2.95 ± 0.15 mg day⁻¹), cocoon numbers (96.0 ± 5.1) and reproduction rate (cocoons worm⁻¹ day⁻¹) (0.034 ± 0.001) in T₂ treatment. In T₃ maximum mortality (30.0 ± 4.01 %) in earthworm population was observed. Overall, T₂ vermibed appeared as an ideal substrate to manage guar gum industrial waste effectively. Vermicomposting can be proposed as a low-input basis technology to convert industrial waste into value-added biofertilizer.     

Impact of industrial air pollutants on some biochemical parameters and yield in wheat and mustard plants

The present study was carried out to determine the impacts of SO₂, NO _x , SPM and RSPM, the most common air pollutants, generated mainly due to industries and vehicles, on some biochemical parameters and yield in wheat and mustard plants during 2006. The concentration of SO₂, NO _x , SPM and RSPM was determined at the polluted sites across the seasons, which ranged between 14.29–18.10, 20.81–22.43, 483.65–500.85 and 160.67–171.18 μg m⁻³, respectively. The wheat and mustard plants grown at polluted sites showed significant reduction in chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll, carotenoid, ascorbic acid, pH, relative water content and yield. The data were further analyzed using a two way ANOVA. It is concluded that the ambient air pollutants have a potential adverse impact on biochemical parameters, which further leads to a reduction in the yield of wheat and mustard crops.     

Responses of Soil CO<Subscript>2</Subscript> Efflux to Precipitation Pulses in Two Subtropical Forests in Southern China

This study was designed to examine the responses of soil CO₂ efflux to precipitation pulses of varying intensities using precipitation simulations in two subtropical forests [i.e., mixed and broadleaf forests (MF and BF)] in southern China. The artificial precipitation event was achieved by spraying a known amount of water evenly in a plot (50 × 50 cm²) over a 30 min period, with intensities ranging from 10, 20, 50 and 100 mm within the 30 min. The various intensities were simulated in both dry season (in December 2007) and wet (in May 2008) season. We characterized the dynamic patterns of soil CO₂ efflux rate and environmental factors over the 5 h experimental period. Results showed that both soil moisture and soil CO₂ efflux rate increased to peak values for most of the simulated precipitation treatments, and gradually returned to the pre-irrigation levels after irrigation in two forests. The maximum peak of soil CO₂ efflux rate occurred at the 10 mm precipitation event in the dry season in BF and was about 3.5 times that of the pre-irrigation value. The change in cumulative soil CO₂ efflux following precipitation pulses ranged from −0.68 to 1.72 g CO₂ m⁻² over 5 h compared to the pre-irrigation levels and was generally larger in the dry season than in the wet season. The positive responses of soil CO₂ efflux to precipitation pulses declined with the increases in precipitation intensity, and surprisingly turned to negative when precipitation intensity reached 50 and 100 mm in the wet season. These findings indicated that soil CO₂ efflux could be changed via pulse-like fluxes in subtropical forests in southern China as fewer but extreme precipitation events occur in the future.     

Nutrient Removal and Loading Rate Analysis of Louisiana Forested Wetlands Assimilating Treated Municipal Effluent

The relationship between nutrient removal and loading rate was examined using data from five forested wetlands in Louisiana that have received secondarily treated effluent from 3 to 60 years. Loading rates ranged from 0.65 to 26.80 g/m²/yr for total nitrogen and 0.18 to 8.96 g/m²/yr for total phosphorus. At loading rates below 20 g/m²/yr, total nitrogen concentrations in surface waters of Louisiana forested wetlands were reduced to background concentrations (i.e., ≤3 mg/l). Similarly, at loading rates below 2 g/m²/yr, total phosphorus concentrations were also generally reduced to background concentrations (i.e., ≤1 mg/l). These data demonstrate that freshwater forested wetlands can reduce nutrient concentrations in treated effluent to background concentrations present in relatively undisturbed wetlands. An understanding of the relationship between loading rates and nutrient removal in natural wetlands is important, particularly in Louisiana where discharges of fresh water are being used in ecosystem restoration.     

Germinable Soil Seed Banks and the Restoration Potential of Abandoned Cropland on the Chinese Hilly-Gullied Loess Plateau

Poor vegetation cover is generally considered to be a major factor causing soil erosion on the Loess Plateau in China. It has been argued that tree planting restoration is ineffective, and natural re-vegetation is an alternative ecological solution for restoring abandoned cropland and controlling soil erosion. The aims of this study were to investigate the characteristics of soil seed banks and to assess the natural restoration potential of abandoned cropland in the hilly-gullied Loess Plateau. The soil seed bank was identified by the germination method with the soil samples, which were collected at four sampling times (April, August, and October 2005 and August 2006) from 12 plots abandoned 3–30 years prior to sampling. The seed bank densities of all of the samples in the 0–10 cm soil layer varied from 1,067 ± 225 to 14,967 ± 1,606 seeds m⁻². Fifty-one species (24 annual and 27 perennial species) belonging to 18 families were identified, and 39% of these species belonged to the families Compositae and Gramineae. The pioneer species Artemisia scoparia dominated the seed bank, with an average seed density of 3,722 seeds m⁻², and accounted for 74.4% of the seeds in the bank. The local dominant species (such as Lespedeza davurica, Artemisia gmelinii, Bothriochloa ischaemun and Stipa bungeana) of the later succession stages also existed at densities varying from 17 to 1, 383 seeds m⁻². The combination of soil seed bank characteristics, reproductive traits of the species, the specific landscape conditions indicates that the potential to restoring the abandoned croplands in the hilly-gullied Loess Plateau via natural re-vegetation could be substantial.     

Arsenic uptake and phytotoxicity of T-aman rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.) grown in the As-amended soil of Bangladesh

A pot-culture experiment was conducted in open-field conditions with highly cultivated locally transplanted (T) aman rice (Oryza sativa L.) named BR-22 in arsenic (As)-amended soil (0, 1.0, 5.0, 10.0, 20.0, 30.0, 40.0 and 50.0 mg kg⁻¹ As) of Bangladesh to see the effect of As on the growth, yield and metal uptake of rice. Arsenic was applied to soil in the form of sodium arsenate (Na₂HAsO₄). Arsenic affected the plant height, tiller and panicle numbers, grain and straw yield of T-aman rice significantly (P ≤ 0.05). The grain As uptake of T-aman rice was found to increase with increase of As in soil and a high grain As uptake was observed in the treatments of 30–50 mg kg⁻¹ As-containing soil. These levels exceed the food hygiene concentration limit of 1.0 mg kg⁻¹ As. However, the straw As uptake varied significantly (P ≤ 0.05) from a low concentration of As in soil (5 mg kg⁻¹) and the highest uptake was noticed in 20 mg kg⁻¹ As treatment.     

Spatial Heterogeneity of Water Quality in a Highly Degraded Tropical Freshwater Ecosystem

Awareness of environmental heterogeneity in ecosystems is critical for management and conservation. We used the Xochimilco freshwater system to describe the relationship between heterogeneity and human activities. This tropical aquatic ecosystem south of Mexico City is comprised of a network of interconnected canals and lakes that are influenced by agricultural and urban activities. Environmental heterogeneity was characterized by spatially extensive surveys within four regions of Xochimilco during rainy and dry seasons over 2 years. These surveys revealed a heterogeneous system that was shallow (1.1 m, SD = 0.4 ), warm (17°C, SD = 2.9), well oxygenated (5.0 mg l⁻¹, SD = 3), turbid (45.7 NTU SD = 26.96), and extremely nutrient-rich (NO₃–N = 15.9 mg l⁻¹, SD=13.7; NH₄–N = 2.88 mg l⁻¹, SD = 4.24; and PO₄–P =  8.3 mg l⁻¹, SD = 2.4). Most of the variables were not significantly different between years, but did differ between seasons, suggesting a dynamic system within a span of a year but with a high resilience over longer periods of time. Maps were produced using interpolations to describe distributions of all variables. There was no correlation between individual variables and land use. Consequently, we searched for relationships using all variables together by generating a combined water quality index. Significant differences in the index were apparent among the four regions. Index values also differed within individual region and individual water bodies (e.g., within canals), indicating that Xochimilco has high local heterogeneity. Using this index on a map helped to relate water quality to human activities and provides a simple and clear tool for managers and policymakers.     

Age dependency of high-affinity ouabain receptors and their magnetosensitivity

Age dependency of [³H]-ouabain binding, ⁴⁵Ca²⁺ eflux and its magnetosensitivity in rats’ brain cortex and heart muscle tissues were studied. Curves of dose-dependent [³H]-ouabain binding consisted of three components with different affinities (10⁻⁷–10⁻⁴ M (α₁); 10⁻⁹–10⁻⁷ M (α₂); and 10⁻¹¹–10⁻⁹ M (α₃)). These curves were also characterized by different dose-dependent kinetics. [³H]-ouabain binding with α₃ receptors in brain cortex and heart muscle tissues of young and adult animals had a dose-dependent character, while that in old ones had a dose-independent character. A 0.2 T static magnetic field (SMF) exposure had modulation effect on ouabain binding with α₁, α₂ and α₃ receptors in young rats, while in adult ones, only α₃ receptors were magnetosensitive. In old animals, SMF exposure had no significant effect on ouabain binding with α₃ receptors in brain cortex, while in heart muscle, it had inhibitory effect on it. Age-dependent effect of ouabain impact on ⁴⁵Ca²⁺ efflux showed that all concentrations of ouabain lead to inhibitory effect in young animals’ brain cortex and heart muscle (with the exception of 10⁻¹⁰ and 10⁻⁶ M), while in old ones, it had activation effect as compared with data received without ouabain. SMF exposure in young animals had activation effect on ⁴⁵Ca²⁺ efflux from brain cortex and heart muscle in data without ouabain, and in old rats, ⁴⁵Ca²⁺ efflux from brain cortex was magnetic insensitive. In old animals, SMF increased ⁴⁵Ca²⁺ efflux even after extra low concentration of ouabain. It is suggested that α₃ receptors having a crucial role in the regulation of Na⁺/Ca²⁺ exchange serve as age-dependent magnetosensors of excitable cells.     

Analysis of Roadside Inhalable Particulate Matter (PM10) in Major Korean Cities

A data analysis of three major Korean cities was conducted to assess roadside inhalable particulate matter 10 μm or smaller in aerodynamic diameter (PM₁₀), including temporal and meteorological variations, over a recent period of 4 to 6 years. The yearly roadside PM₁₀ concentrations presented a well-defined increasing trend or no trend depending on the roadside monitoring station. Most mean values exceeded or approximated the Korean standard of 70 μg/m³ per year for PM₁₀. A representative roadside diurnal trend was characterized by a distinct morning maximum. In most cases, the Sunday roadside concentrations were similar to or somewhat lower than the weekday concentrations, and the PM₁₀ concentrations presented a well-defined seasonal variation, with the maximum concentration in March. The monthly maximum concentrations observed in March were most likely attributable to Asian dust storms. In two metropolitan cities (Seoul and Busan), the frequency of days with roadside PM₁₀ concentrations exceeding the standard of 150μg/m³ per 24 h was much lower for the roadside monitoring stations than for the residential monitoring station, whereas in the third city (Daegu), this result was reversed. Interestingly, the average maximum concentrations observed for the roadside sites in Seoul and Busan during March were higher than those for the residential sites, suggesting that the roadside concentrations responded more to the dust storms than the residential areas. The relationship between the pollutant concentrations and five important meteorological parameters (solar radiation, wind speed, air temperature, relative humidity, and precipitation) showed that the number and type of meteorological variables included in the equations varied according to the monitoring station or season. Finally, the current results confirmed that attention should be given to the PM₁₀ exposure of residents living near roadways.