Chemical exergy based evaluation of water quality

The thermodynamic concept of chemical exergy is introduced for water quality evaluation, to develop unified objective indicators in contrast to conventional indicators characteristic of subjectivity. While a quantity termed specific standard chemical exergy based on the global reference substances is used to evaluate the standard water quality, an indicator as specific relative chemical exergy with reference to a spectrum of substances associated with some specified water quality standard is developed for practical water quality evaluation, with related concepts of carrying deficit and carrying capacity well embodied in exergy terms. Based on the data collected in the GEMS/WATER project, water qualities of 72 rivers and 24 lakes over the world are evaluated, as a detailed case study to illustrate the adaptability of the chemical exergy based indicators for water quality evaluation.     

Exergy vs information in ecological successions: Interpreting community changes by a classical thermodynamic approach

This work proposes a methodology based on classical thermodynamics, which allows the variation in ecosystem composition to be interpreted within the framework of the exergy concept. The basic equation of exergy [Mejer, H., Jorgensen, S.E., 1979. Exergy and ecological buffer capacity. State-of-the-art in Ecological Modelling 7, 829–846] was decomposed into three terms – size (C), structural information (I) and concentration (X) – and their significance as indicators of ecosystem state was evaluated by simulating different scenarios of development in a simplified freshwater ecosystem. In order to calculate the exergy terms, the most critical issue in using exergy in an ecological context, i.e. the estimate of reference equilibrium values for organic matter and organisms, had to be faced. With this aim, the equations of classical thermodynamics in solution were applied, and “virtual” values of concentration at equilibrium were calculated for a number of organic compounds (VEC) and freshwater organisms (VECE). The results of the simulation showed that, whereas exergy and the exergy terms inherently connected with the a-biotic component varied consistently with the incorporation of biomass into the ecosystem, the structural information of the biotic component followed different, even opposite, pathways of variation, which were dependent only on the change in the size spectrum of the community. Due to the strict dependence of the VECE values on organism size, the increase of structural information with increasing abundance of large and complex species is also consistent with the general pattern of succession delineated by the classical r–K model. Structural information is therefore proposed as an indicator of the development state, as well as an ecological orientor, whose maximisation is expected during ecosystem development. However, since an increase in structural information is not necessarily accompanied by an increase in exergy, a sort of “antagonism” between these two related orientors emerges, whose resolution may contribute to shed light on the fundamental forces which drive ecosystem development.     

Phytotoxicity in seven higher plant species exposed to di-<Emphasis Type="Italic">n</Emphasis>-butyl phthalate or bis (2-ethylhexyl) phthalate

We investigated phytotoxicity in seven plant species exposed to a range of concentrations (0–500 mg·kg^?1 soil) of di-n-butyl phthalate (DnBP) or bis (2-ethylhexyl) phthalate (DEHP), two representative phthalate esters (PAEs) nominated by USEPA as priority pollutants and known environmental estrogens. We studied seed germination, root elongation, seedling growth, biomass (fresh weight, FW) and malondialdehyde (MDA) content of shoots and roots of wheat (Triticum aestivum L.), alfalfa (Medicago sativa L.), perennial ryegrass (Lolium perenne), radish (Raphanus sativus L.), cucumber (Cucumis sativus L.), oat (Avena sativa) and onion (Allium cepa L.), together with monitoring of plant pigment content (chlorophyll a, b and carotinoids) in alfalfa, radish and onion shoots. Root elongation, seedling growth and biomass of the test species were generally inhibited by DnBP but not by DEHP, indicating a lower level of phytotoxicity of DEHP than of DnBP. MDA contents of four species were promoted by PAE exposure, but not in alfalfa, ryegrass or onion shoots, indicating lower sensitivity of these three species to PAE pollutants. Plant pigment contents were clearly affected under the stress of both pollutants, implying the potential damage to the photosynthetic system of test plants, mainly by decreasing the content of chlorophyll a and b. Results of DnBP and DEHP phytotoxicity to the primary growth of test plants has provided information for the assessment of their environmental risk in the soil and also forms a basis for the further analysis of their toxic effects over the whole growth period of different plant species.     

Carbon sequestration potential of Rhizophora mucronata and Avicennia marina as influenced by age, season, growth and sediment characteristics in southeast coast of India

This work analysed the carbon sequestration potential in two species of mangroves (Rhizophora mucronata and Avicennia marina) along with their growth, biomass, sediment characteristics for four seasons of the year 2009–2010, in planted stands of different age (1–17.5 years) in the Vellar-Coleroon estuarine complex, India. The mangroves were recorded to store significant amount of biomass. Avicennia marina performed better to display 75 % higher rate of carbon sequestration than that in Rhizophora mucronata. This could be attributed to growth efficiency and high biomass production. For instance, Avicennia marina exhibited 2.7 fold higher girth, 24 % higher net canopy photosynthesis, 2 fold aboveground biomass (AGB), 40 % more belowground biomass (BGB) and 77.3 % higher total biomass, than R. mucronata did. Seasonally the rate of carbon sequestration was 7.3 fold higher in post-monsoon, 3.4 fold in monsoon, 73 % more in summer than that in pre-monsoon. The rate of carbon sequestration was positively correlated with age of planted site, tree height, tree diameter, net canopy photosynthesis, AGB, BGB, total biomass, carbon stock, growth efficiency, AGB/tree height tree girth, leaf area index, silt content (p?<?0.01). The carbon sequestration was negatively corrected with soil temperature and clay content (p?<?0.05). Mangroves were found to be a productive system and important sink of carbon in the tropical coastal zone, but increasing soil temperature due to global warming would have a negative impact on carbon sequestration potential of the mangroves.     

Rythmes nutritionnels et organisation trophique d'une population de crustacés pélagiques (Euphausiacea)

The nature of the food (animal, plant or mixed) and the fullness of the stomachs at different times of the day have been studied through dissection of 18620 specimens representative of almost all the euphausiid species of the Central and Western Tropical Pacific Ocean. Animal food predominates in 22 of the 28 species studied, while 12 can be considered as omnivorous; only 4 are mainly phytophagous. The trophic level of a given species is more or less the same in different zones, but scarcity of phytoplankton in oligotrophic tropical regions results in an increase of the trophic level of herbivorous and omnivorous species. There is no correlation between trophic level and vertical distribution of a species. Each species follows a clearly defined feeding rhythm, usually characteristic for each genus: nutrition most active by night in Euphausia, continuous in Thysanopoda, restricted to light hours in Stylocheiron, mainly from noon to midnight in Nematoscelis and Nematobrachion. As a rule, it is obvious that the smaller the daily vertical migration, the more pronounced the feeding rhythm: the range of fluctuations in fullness of stomachs over 24 h is weak or non-existent in migrating species, maximum in non-migrating ones. Nevertheless, daily vertical migration does not appear to be “advantageous” from the point of view of trophic efficiency: assuming that the stomachal transit is the same for all species (a speculative proposition), it is shown that the ratio “total biomass of species: food consumed during 24 h”, i.e., “biomass permanently available for the upper link: biomass eaten daily at the expense of the lower link” is 4 times higher in non-migrating than in migrating species. It is thus considered that daily vertical migration is an expensive manner to transfer energy from link to link, and therefore fulfills other functions, amongst which diffusion throughout the whole water column of the biomass produced in the upper levels is probably one of the most important.     

The role of microbial mats during primary succession in calcareous dune slacks: an experimental approach

Laminated microbial mats from a sandy beach plain were grown in water-saturated pots in a glass house for six months and then used to assess their effect on the establishment of juveniles of three plant species representing different successional stages in dune slack development. The selected species wereSamolus valerandi, characteristic of pioneer stages,Calamagrostis epigejos, characteristic of more productive, late successional stages, andJuncus alpinoarticulatus, which occurs in a wide range of successional stages. Juveniles of all three species that were placed on top of intact living microbial mats established themselves in the mat.C. epigejos andJ. alpinoarticulatus survived for several weeks but later on their numbers decreased and the total biomass production of the species after six months was poor.S. valerandi, in contrast, grew profusely in intact microbial and algal mats. Heating of the microbial mat by heat sterilization, prior to the experiment, did not improve the performance of the species. When the juveniles were planted in the microbial mats after breaking the surface of the mat, the survival of juveniles ofC. epigejos andJ. alpinoarticulatus was much higher and so was the biomass of surviving plants after six months. Planting ofSamolus in the mats had some positive effect on the survival percentage of the juveniles, but not on the total biomass at the end of the experiment. Slightly lower water tables had a negative effect on the performance of all species. Measurements of the pH in the pots revealed that there were no significant differences in the top layer. Sulphide concentrations were very low in all the pots where juveniles had been planted and also in the pots withS. valerandi. Relatively high concentrations (30–50 μmol/l) were found in pots with poor growth ofJuncus andCalamagrostis plants. These values may exceed toxic levels for these species. Although oxygen concentrations in the pots were generally low, no relation existed between plant biomass and oxygen content, indicating that plant growth was not primarily limited by oxygen stress. These experiments support the idea that microbial mats may assist in extending the life span of early pioneer stages during dune slack succession by inhibiting the growth of species of later successional stages.     

Geochemical and ecological significance of soil lipids under Rhododendron ponticum stands

The bio-geographical significance of Rhododendron ponticum spp. baeticum (Ericaceae) as a relict species is well recognized. However, out of its native habitat it is an invasive exotic considered a major threat to natural ecosystems in areas of Atlantic Western Europe. The studies on the impact of Rhododendron influence on soil organic matter composition and associated ecological implications, i.e. presence of bioactive compounds with ecological significance, are limited. This work describes the soil lipid assemblage in three sites under Rhododendron stands and adjacent sites with deciduous oak (Quercus canariensis), both in their native habitats in Southern Spain (Sierra de Luna, Cádiz). The results are discussed in terms of organic matter dynamics and the presence of molecules that may be associated with Rhododendron invasive success. The soils are acid Xerochrepts formed on siliceous sands. Composite soil samples were taken at two depths (0?C10?cm and 10?C20?cm) and soxhlet extracted with a dichloromethane-methanol mixture (3:1). Soil lipid assemblage was studied by GC/MS after fractionation and appropriate derivatization of extracts. The qualitative chemical composition of soil extractable lipids under Rhododendron is reported here for the first time. Our results show that soil n-alkane and fatty acid distributions are compatible with an input from plant epicuticular waxes, as well as with the occurrence of selective preservation of long-chain fatty acids with depth. The pattern of short-chain n-alkanes found in surface samples indicates an anthropogenic contamination threat from nearby industrialized areas of ??Campo de Gibraltar??. The presence of branched iso and anteiso C₁₅ and C₁₇, ??-hydroxy fatty acids and the sterol brassicasterol points to high microbial soil activity. Finally, the pentacyclic triterpenes taraxerone and taraxerol were detected in soils with Rhododendron but not with Quercus. These are known bio-active plant compounds and could be related with the effectiveness of Rhododendron as an invasive exotic species.     

Impact of organic manure on the phytoremediation potential of Vetiveria zizanioides in chromium-contaminated soil

Chromium is a pollutant present in electroplating waste water and its removal is necessary for the protection of the environment. Vetiveria zizanioides (VZ) was grown in chromium effluent concentrations of 50, 100 and 200 mg kg ^?1 soil amended with organic manure and the potential for phytoremediation was determined. The amounts of Cr in plant tissues (root and shoot), soil and percentage electrolyte leakage of VZ roots were analysed. From the results, VZ amended with organic manure showed the greatest potential for Cr removal because of its faster growth and larger biomass achieved over the whole length of the experiment. In this study, 92.25% Cr removal efficiency was obtained with a Cr concentration of 50 mg kg ^?1 soil and removal efficiencies of 90.5% and 85% were obtained with 100 and 200 mg kg^?1, respectively after a period of two months of VZ growth.     

Effectiveness of emergent and submergent aquatic plants in mitigating a nitrogen–permethrin mixture

Hydraulically connected wetland microcosms (～50?L) in series were used to test the effectiveness of varying combinations of two common aquatic vascular plants, parrot feather (Myriophyllum aquaticum) and cattail (Typha latifolia), for mitigating contamination from a mixture of nitrogen (ammonium nitrate) and permethrin. The upstream series included Myriophyllum only (M) and Typha only (T) while the combination upstream effluent into downstream series included Myriophyllum into Myriophyllum (M?+?M) and Typha into Myriophyllum (T?+?M). During flow, M into M?+?M more efficiently mitigated nitrogen than T into T?+?M. Post-flow, nitrogen removal efficiency was greater for T versus M and M?+?M versus T?+?M. Mean aqueous dissipation half-lives (t_1/2) of NH₄-N and NO₃-N were more rapid in T than M treatments. Ammonium and nitrate t_1/2 was highly correlated with aquatic plant above-ground shoot biomass. Permethrin mitigation efficiencies and t_1/2 were not significantly affected by plant species either singly or in combination. Trans-permethrin t_1/2 was moderately correlated with plant biomass, but not cis-permethrin t_1/2. Results of this study indicate differences in aquatic plant species and flow path influence nitrogen removal but not permethrin. However, plant species appears less important than overall plant biomass in ascertaining aquatic plant effectiveness in mitigating a nitrogen–permethrin mixture.     

Plant diversity reduces the effect of multiple heavy metal pollution on soil enzyme activities and microbial community structure

It is unclear whether certain plant species and plant diversity could reduce the impacts of multiple heavy metal pollution on soil microbial structure and soil enzyme activities. Random amplified polymorphic DNA (RAPD) was used to analyze the genetic diversity and microbial similarity in planted and unplanted soil under combined cadmium (Cd) and lead (Pb) pollution. A metal hyperaccumulator, Brassica juncea, and a common plant, Festuca arundinacea Schreb, were used in this research. The results showed that microorganism quantity in planted soil significantly increased, compared with that in unplanted soil with Cd and Pb pollution. The order of microbial community sensitivity in response to Cd and Pb stress was as follows: actinomycetes>bacteria>fungi. Respiration, phosphatase, urease and dehydrogenase activity were significantly inhibited due to Cd and Pb stress. Compared with unplanted soil, planted soils have frequently been reported to have higher rates of microbial activity due to the presence of additional surfaces for microbial colonization and organic compounds released by the plant roots. Two coexisting plants could increase microbe population and the activity of phosphatases, dehydrogenases and, in particular, ureases. Soil enzyme activity was higher in B. juncea phytoremediated soil than in F. arundinacea planted soil in this study. Heavy metal pollution decreased the richness of the soil microbial community, but plant diversity increased DNA sequence diversity and maintained DNA sequence diversity at high levels. The genetic polymorphism under heavy metal stress was higher in B. juncea phytoremediated soil than in F. arundinacea planted soil.     

Impact of anaerobically treated and untreated (raw) distillery effluent irrigation on soil microflora, growth, total chlorophyll and protein contents of Phaseolus aureus L

Impact of distillery effluent (untreated and treated) irrigation on soil microflora of the pots used for growing Phaseolus aureus L. was investigated. The growth of the P. aureus plants as affected by distillery effluent irrigation was also evaluated. The irrigation of the pots by 1-10% distillery effluent (anaerobically treated) stimulated the growth of the soil microflora (increased number of bacteria, fungi and actinomycetes) and P. aureus plants (increased shoot and root lengths, biomass, chlorophyll and protein contents). Further, 15-20% distillery effluent (anaerobically treated) had toxic effect on soil micro flora as indicated by reduced number of bacteria, fungi and actinomycetes. Reduction in shoot, root, lengths, biomass, chlorophyll, protein contents of P. aureus was also observed when irrigated by 15-20% treated distillery effluent. All the concentrations of raw distillery effluent reduced the bacterial population. However, the treated distillery effluent concentrations <10% had stimulatory effect on fungal and actinomycetes population. However, raw effluent concentrations >5% reduced the same. Raw distillery effluent was more toxic to P. aureus than treated distillery effluent as concentrations >5%, had reduced the growth (shoot, root length and biomass) of the test plant. Raw distillery effluent had adverse effect to total chlorophyll contents and all the test concentrations reduced the total chlorophyll level. However, untreated (raw) distillery effluent stimulated the protein content initially. It has been concluded from-present study that lower concentrations of the raw distillery effluent (1-5%) and treated distillery effluent (1-10%) had stimulated the growth of P. aureus and soil microflora except soil bacteria (inhibited by all the concentration of the raw effluent). However, higher concentrations (raw effluent: 10-20%; treated effluent 15-20%) had toxicity to test parameters.     

Exergetic assessment for ecological economic system: Chinese agriculture

Based on the thermodynamic concept of exergy as a unified measure for environmental resources and economic products, a framework for systems assessment is presented for ecological economies. With a typical systems diagram devised for a general ecological economy with four arm fluxes for free local natural resources, purchased economic investment, environmental impact and economic yield, system indices of the renewability index, exergy yield ratio, exergy investment ratio, environmental resource to yield ratio, system transformity and environmental stress index are defined for a congregated systems ecological assessment with essential implications to sustainability. As a detailed case study to the Chinese agriculture from 1980 to 2000 with cropping, forestry, stockbreeding and fishery sectors, extensive exergy account and systems assessment are carried out with emphasis on annual and structural variations against social political transitions. For the overall agriculture as a congregated ecological stage, the value of the system transformity is found around 10, the typical value for the general ecological hierarchy as well devised by Odum associated with Lindeman's Tenth Law.     

Phytotoxicity of fuel to crop plants: influence of soil properties,fuel type,and plant tolerance

The aim of the present study was to determine the effect of fuel-contaminated soils on the germination, survival, and early growth of six crop plants, viz. Brassica oleracea, Trifolium repens, Lactuca sativa, Avena sativa, Pisum sativum, and Zea mays, grown on Cambisol A and B horizons contaminated with gasoline and diesel (0%, 1.25%, 2.5%, 5%, and 10%, w/w). Fuel toxicity was more evident in the B horizon than in the A horizon, and diesel was more toxic than gasoline, probably due to the higher evaporation rate of the latter. Fuels affected the germination and survival of small-seeded plants to a higher extent, reflecting the importance of the seed coat and nutrient reserves for successful plant development in fuel-contaminated soils. In general, root growth was more strongly affected than shoot growth, and plant biomass was more strongly affected than elongation, leading to a less plant branching in the presence of fuel. The findings of this study can be useful for selecting the least fuel-tolerant species as soil contamination bioindicator and for determining the risks of fuel contamination. Due to the low residence time of gasoline components in soil, this phytotoxicity test resulted in an unsuitable bioassay to assess gasoline toxicity.     

Assessment of the pulp and paper mill effluent on growth,yield and nutrient quality of wheat (Triticum aestivum L.)

Assessment of agropotentiality of the effluent coming out from century pulp and paper mill, Ghanshyamdham, Lalkua (Uttaranchal) has been made on wheat (Triticum aestivum var. UP-2329) crop grown in two soils differing in texture with different effluent concentrations. Diluted effluent increased the chlorophyll content, plant height, shoot and root biomass, grain yield, protein, carbohydrate and lipid contents in wheat grains, while undiluted effluent caused inhibition in plant growth resulting in a sharp decline of yield. Pure soil provided better growth and yield results than those soil mixed with sand.     

Allocation of nitrogen and carbon in an estuarine salt marsh in Portugal

Above and below-ground biomass and nitrogen and carbon composition ofSpartina maritima, Halimione portulacoides andArthrocnemum perenne, dominating species in plant communities of the lower, middle and higher salt marsh, respectively, were compared in an estuarine salt marsh in Portugal. Plant and soil nitrogen and carbon pools were estimated. For all three species root biomass was significantly higher (70–92% of total biomass) than above-ground biomass. The percentage of root biomass was related to the location of the plants in the marsh: higher values were found in plants growing in the lower salt marsh where the sediment was more unstable and subject to tidal action, which stresses the role of the roots as an anchor. For all three species nitrogen concentrations were highest in leaves, reflecting the photosynthetic role of the tissue. For carbon higher concentrations were found in the stems, with the exception ofS. maritima. In general, lower nitrogen concentrations were found in summer, which can be explained by dilution processes due to plant growth. For both nitrogen and carbon, higher concentrations were found in the soil surface layers. Higher soil nitrogen and carbon levels were associated with higher organic matter contents. Most of the nitrogen in the salt marsh occurred in the sediments (0–40 cm) and only ca. 5.7–13.3% of the total was found in the plants. The greater portion (76.5%–86%) of carbon was found in the sediment.     

Arsenic,antimony, and other trace element contamination in a mine tailings affected area and uptake by tolerant plant species

The study was conducted to characterize mineralogical and elemental composition of mine tailings in order to evaluate the environmental hazards, and identify the metal accumulation potential of native plant species from São Domingos mine, one of the long-term activity mines of the Iberian Pyrite Belt dating back to pre-Roman times. The mine tailings including soils and different plant species from São Domingos were analyzed for determination of tailings characteristics and chemical element contents in tailings and plants. The large amounts of mining wastes are causing significant adverse environment impacts due to acid mine drainage production and mobilization of potentially toxic metals and metalloids in residential areas, agricultural fields, downstreams, and rivers. The typical mineralogical composition is as follows: quartz, micas, K-feldspar, olivine-group minerals, magnetite, goethite, hematite, jarosite, and sulfides. The mine tailings were highly contaminated by As, Ag, Cr, Hg, Sn, Sb, Fe, and Zn; and among them, As and Sb, main contaminants, attained the highest concentrations except Fe. Arsenic has exhibited very good correlations with Au, Fe, Sb, Se, and W; and Sb with As, Au, Fe, Se, Sn, and W in tailings. Among the all plant species, the higher concentrations of all the metals were noted in Erica andevalensis, Erica australis, Echium plantagium, and Lavandula luisierra. Considering the tolerant behavior and abundant growth, the plant species Erica australis, Erica andevalensis, Lavandula luisierra, Daphne gnidium, Rumex induratus, Ulex eriocladus, Juncus, and Genista hirsutus are of major importance for the rehabilitation and recovery of degraded São Domingos mining area.     

Impact of pedological conditions on the distribution of Salicornia species (Southern North Sea coast)

The zonation of different Salicornia species within saltmarshes has been described controversially in prior publications. The aim of this study is to detect substantial relations between pedological conditions and the distribution of different Salicornia species (Salicornia stricta Dumort. 1868, Salicornia europaea L. 1753, and Salicornia procumbens Sm 1813). 37 sites were investigated regarding vegetation pattern, soil morphology and physicochemical and chemical soil properties. A multivariate approach indicate a clear differentiation of the sites dominated by different Salicornia species in respect to sand content, redox potential and water content. However, results of a detailed statistical assessment indicate that redox potential and salinity are the most relevant parameters. Salicornia stricta sites are characterized by a variety of grain sizes, reduced conditions in the rhizosphere, highest water contents (median: 71.5 Vol-%) and lowest Eh values (median: ?19.0 mV), indicating the best adaptation to anaerobic soil conditions. Salicornia procumbens occurs on aerated soils (median Eh: 455.6 mV) with high sand contents independent to position relative to mean high tide level. Exhibiting the lowest (11.5‰) and highest soil salinities (41.7‰), even developing in salt pans (136‰), Salicornia europaea feature a great ecological amplitude regarding temporal variations of salinity. These findings underline the ecotypic and phenotypic plasticity of Salicornia species and offer a promising approach for further experimental settings focussing on phenotypic adaptions and ecological amplitudes of Salicornia taxa and ecotypes.     

Current developments in soil ecotoxicology and the need for strengthening soil ecotoxicology in Europe: results of a stakeholder workshop

Background

Soil is one of our most important resources and fulfills many ecological functions such as storage and filtration of water and nutrients, transformation of chemical compounds and nutrients, biomass production, and carbon storage. Such soil functions support ecosystem services provided by soils, which need to be protected to protect soil fertility. However, European soils often contain elevated concentrations of contaminants, putting biodiversity of soil organisms as well as the ecological functions and services at risk. To promote soil ecotoxicology in Switzerland, the Swiss Centre for Applied Ecotoxicology together with the Federal Office for Environment and the Federal Office for Agriculture organized a stakeholder workshop on 7 June 2018 with participants from research, governmental bodies, and associations. One goal of this workshop was to inform participants about currently available risk assessment approaches for soil, the soil risk assessment for plant protection products in Europe, available bioassays and bioindicators, and results of research projects on soil contaminants in Switzerland. Another goal was to discuss the needs for research in soil ecotoxicology in Switzerland and to identify next steps, potential projects, and future collaborations.

Results

The main needs identified during the workshop were the establishment of (bio)indicators to measure soil fertility, functional parameters to determine soil functions, and the preservation of soil biodiversity. Another priority listed was the formation of a working group, which addresses the issue of the development of environmental quality standards for soil. The need for experimental field sites for implementing and testing new approaches or tools for assessing soil quality was also discussed.

Conclusion

The next steps planned are two workshops with national and international experts in soil ecotoxicology to develop a soil monitoring concept for Switzerland and to find suitable bioindicators to evaluate soil fertility. Additionally, a literature review will be performed summarizing the current ecotoxicological state of the art with regard to the development of bioindicators in relation to the monitoring of plant protection products in Swiss soil, to evaluate their effects on soil fertility. Furthermore, all attendees agreed on the need for annual meetings or workshops where experts can present scientific results, participants can exchange information, and future projects and collaborations can be developed.

     

Distribution of arsenic compounds in Plantaginaceae and Cyperaceae plants growing in contaminated soil

The ability of plant species to accumulate arsenic (As) species in the biomass from As-contaminated soils is variable. Among the plants widely grown at the As-contaminated locations, Plantaginaceae and Cyperaceae families belong to the frequent ones. In this study, the ability of Plantago lanceolata (Plantaginaceae) and three wetland plant species representing the family Cyperaceae (Carex praecox, Carex vesicaria, and Scirpus sylvaticus) naturally occurring in the soils with an elevated As in the Czech Republic were investigated. The plants were cultivated under controlled conditions in an As-contaminated soil reaching 735?mg?kg^?1 of the total As. The total As in plants reached up to 8.3?mg?kg^?1 in leaves, and up to 155?mg?kg^?1 in roots of C. praecox. Dominant As compounds were arsenite and arsenate with a small abundance of dimethylarsinic acid (DMA) in all the plant species. In Cyperaceae, small percentages of arsenobetaine (AB) and arsenocholine (AC) were detected, suggesting the ability of these plants to transform As into less toxic compounds. Moreover, the important role of As(V) sequestration on iron plaque on the root surface of Cyperaceae was confirmed. In this context, root washing with oxalic acid partially disrupted the iron plaque for the better release of arsenate.