Seasonal variation in ecophysiological patterns in macroalgae from an Arctic fjord. I. Sensitivity of photosynthesis to ultraviolet radiation

Marine macroalgae inhabiting Arctic coastal ecosystems are exposed to pronounced seasonal variations in the radiation regime, including harmful UVB radiation. This study presents the first data on the seasonal changes in the sensitivity of macroalgal photosynthesis towards UV exposure by comparing under-ice, clear-water and turbid-water conditions characteristic for late winter, spring and summer. Various brown (Laminaria saccharina, L. digitata, L. solidungula, Saccorhiza dermatodea, Desmarestia aculeata), red (Palmaria palmata, Devaleraea ramentacea) and one green macroalgal species (Monostroma aff. arcticum) were collected at the same water depth throughout the seasons in the Kongsfjord (Spitsbergen, Svalbard, Norway). Maximum quantum yield (F_v/F_m) and maximum photosynthetic electron transport rates (ETR_max) were determined immediately after collection, after 2 h exposure to artificial UV radiation and after 18 h recovery in dim white light. Photosynthesis of the studied species showed different responses depending on their morpho-functional and physiological characteristics, their life strategies, phenology and depth distribution. Within the genus Laminaria, maximum quantum yield of adult specimens of the deep-water species L. solidungula was most strongly UV sensitive. Adult L. saccharina exhibited a lower UV sensitivity than a 6-month-old specimen. Inhibition of photosynthesis after UV exposure remained at the same level throughout the study period, both in adult L. saccharina and S. dermatodea. However, adult specimens of L. saccharina collected in May showed partial recovery only, whereas photosynthesis of specimens from both species collected later recovered fully. D. aculeata exhibited a remarkable decrease of UV sensitivity during the study period. Photosynthesis of specimens collected under the ice was strongly inhibited by UV, but the degree of inhibition decreased during spring and summer. Concomitantly ETR_max values were low after UV exposure in specimens collected in June, but increased later in the season. P. palmata exhibited a relatively flexible response. Photosynthesis in specimens collected under the ice in June or in turbid water in July/August was relatively strongly inhibited; specimens collected during sunny periods and in clear water in spring showed a much lower degree of photoinhibition after UV exposure. The seasonal pattern of low/high ETR_max values in spring/summer is probably a characteristic of the life strategy of this species. The UV sensitivity of D. ramentacea exhibited a similar seasonal pattern. In M. aff. arcticum, UV sensitivity increased and ETR_max values decreased during the study period, reflecting the life strategy of this annual late winter/spring species. The physiological basis for the seasonal changes in UV sensitivity of photosynthesis is presented in a companion paper (this issue).     

Seasonal variations in salt-march macroalgae photosynthesis. II. Fucus vesiculosus and Ulva lactuca

Photosynthesis in whole plants of the salt-marsh algae Fucus vesiculosus and Ulva lactuca was evaluated by ¹⁴C-uptake under a variety of light intensities at approximately mately monthly intervals during a 15-month study. Photosynthetic capacity in both species was closely related to seasonal irradiation patterns and changes in field biomass. Maximum photosynthesis occurred in the spring and summer months. Photosynthesis on a dry weight basis was higher in U. lactuca, while photosynthesis on a chlorophyll a basis was equal in both species. Photosynthetic capacity was inversely related to pigment content. Maximum chlorophyll a concentrations occurred during the winter. Frond profile studies in F. vesiculosus indicated that apices always exhibited greatest photosynthetic capacity. Uptake of ¹⁴C into ethanol-soluble and insoluble fractions was different in each species. F. vesiculosus showed greater activity in the ethanol-soluble fraction while U. lactuca exhibited greater activity in ethanol-insoluble fractions.This research was supported by Research Grant AG-375 from the National Science Foundation and, in part, by the State University of New York Research Foundation and the Energy Research and Development Administration (ERDA).     

Chemical defence in ladybird beetles (Coccinellidae). II. Amount of reflex fluid,the alkaloid adaline and individual variation in defence in 2-spot ladybirds (Adalia bipunctata)

Summary 2-spot ladybirds secrete alkaloid (adaline)-rich defence fluid (reflex blood) in response to predator attack. Reflex fluid was collected from individual ladybirds and weighed and the alkaloid content measured by GC. The amount of fluid produced built up rapidly following winter hibernation in animals feeding on aphids. The concentration of adaline in the fluid was highest in the first bleeding after winter hibernation. A large sample of beetles was reflex bled several times. Significant among beetle variation was found in the amount of fluid produced and the concentration of the reflex blood. The results are discussed in relation to the possibility that 2-spot ladybirds are Batesian mimics of 7-spot ladybirds and to the possible functions of adaline.