Crustacean epifauna of seagrass and macroalgae in Apalachee Bay,Florida, USA

Epifaunal crustaceans on turtlegrass (Thalassia testudinum) and five dominant macroalgae (Anadyomene stellata, Digenia simplex, Halimeda incrassata, Laurencia poitei and Penicillus lamourouxii) were quantitatively sampled bimonthly over a one-year period from September 1979 to September 1980 in a subtropical seagrass meadow in Apalachee Bay, Florida (northeastern Gulf of Mexico). These plant species exhibited a wide range of morphologies, with surface area-to-biomass ratios differing by over 2.5 times. A similar suite of crustaceans occurred on all macrophytes despite differences in shape or architecture among plant species. Relative abundances of many crustaceans, however, varied among plant hosts. Similarity analysis indicated that the epifaunal associates of T. testudinum were distinct from those of the macroalgae. Species richness was generally higher on turtlegrass than on any of the macroalgae. Abundances of total crustaceans per plant biomass or per plant surface area, on the other hand, were greater on all macroalgal species compared to the seagrass. Abundances (per plant biomass or plant surface area) of 14 of the 16 numerically dominant epifaunal species differed significantly among macrophytes. Twelve of the 16 species had greater abundance on one or more macroalgae, while only two species were more abundant on T. testudinum. Almost half of the dominant species had greatest abundances on the branching red alga L. poitei. Although abundances per plant biomass and plant surface area were greater on macroalgae relative to turtlegrass, densities (individuals per meter square of bottom) of animals associated with T. testudinum were significantly greater than those associated with macroalgae, primarily because of the greater abundance of turtlegrass in the grass bed. Both surface area-to-biomass ratios and degree of branching were poorly correlated with epifaunal abundance and number of species. Neither structural feature is an adequate predictor of faunal abundance and species richness among plant species, especially when macrophytes with very different morphologies are compared.     

Nitrogen versus phosphorus limitation in a subtropical coastal embayment (Moreton Bay; Australia): Implications for management

An approach combining nutrient budgets, dynamic modelling, and field observations of phytoplankton and nitrogen (N₂)-fixing Lyngbya majuscula following changes in wastewater N loads, was used to demonstrate that Moreton Bay is potentially phosphorus (P) limited. Modelling and nutrient budgeting shows that benthic N-fixation loads are high, allowing the system to overcome any potential N-limitation. Phytoplankton biomass has shown little change from 1991 to 2006 in the sections of Moreton Bay most impacted by wastewater effluents, despite a large reduction in wastewater N loads from 2000 to 2002. This is consistent with modelling that also showed no reduction in primary productivity associated with reduced N loads. Most importantly, there have been rapid increases in the occurrence of N-fixing L. majuscula in Moreton Bay as wastewater P loads have increased relative to wastewater N loads. This is also consistent with modelling. This work supports the premise that there may be fundamental differences in nutrient limitation of primary production between subtropical and temperate coastal systems due to differences in the importance of internal nitrogen sources and sinks (N-fixation and denitrification). These differences need to be recognised for optimum management of coastal systems.     

Uptake and release of phosphorus by phytoplankton in the Chesapeake Bay estuary,USA

The phytoplankton uptake and release rates for inorganic phosphate, dissolved organic phosphate and polyphosphate were estimated during 5 cruises on the Chesapeake Bay over a 9-month period. Phosphorus in all pools turned over in several minutes to 100 h, and each soluble pool appeared to contain fractions which were metabolically useful to the phytoplankton. Maximal uptake rates (V _m ) for orthophosphate ranged from 0.02 to 2.95 μg-at P (1.h)^-1 with half saturation constants (K _s ) between 0.09 and 1.72 μg-at P l^-1. At low soluble reactive phosphorus concentrations, the uptake rate of trace ³²P orthophosphate was initially rapid, but declined after 15 to 60 min incubation. The data suggest that the initial uptake phase was dominated by exchange of ³²PO₄ ^≡ for ³¹PO₄ ^≡ in the membrane transport systems whereas the subsequent phase represented the net incorporation of orthophosphate into phytoplankton cells.     

The distribution of,and relation among,mercury and methylmercury,organic carbon,carbonate, nitrogen and phosphorus,in periphyton of the South Florida ecosystem

Periphyton samples from Water Conservation Areas, Big Cypress National Preserve, and Everglades National Park in south Florida were analyzed for concentrations of total mercury, methylmercury, nitrogen, phosphorus, organic carbon, and inorganic carbon. Concentrations of total mercury in periphyton decrease slightly along a gradient from north‐to‐south. Both total mercury and methylmercury are positively correlated with organic carbon, nitrogen and phosphorus in periphyton. In horizontal sections of periphyton mats, total mercury concentrations tend to be largest at the tops and bottoms of the mats. Methylmercury concentrations tend to be the largest near the bottom of mats. These localized elevated concentrations of methylmercury suggest that there are “hot spots”; of methylmercury in periphyton.     

Nitrate metabolism in sediments from seagrass (Zostera capricorni) beds of Moreton Bay,Australia

The fate of nitrate in sediments from seagrass (Zostera capricorni Aschers.) beds of Moreton Bay on the subtropical eastern coast of Queensland, Australia, was investigated. Added nitrate was metabolised at rates of 0.4 to 3.4 g N cm^-3 d^-1 when sediments were incubated under anaerobic conditions with a large excess of nitrate. The potential rate of nitrate utilization was as rapid in sediments from subtidal bare areas as from adjacent seagrass beds. Ammonium was produced rapidly from¹⁵N-nitrate by microbial action in all the subtidal sediments examined. After 12 h of incubation, 13 to 28% of the¹⁵N initially added as labelled nitrate was detected as labelled ammonium in the sediments. Denitrification, although not measured directly, appeared to be a relatively minor fate of nitrate. Benthic microbes took up large amounts of¹⁵N but only after a delay of 6 h; this pattern could have been due to induction and synthesis of the enzymes necessary for nitrate uptake, and the assimilation of labelled ammonium. Under field conditions, assimilation by seagrasses and denitrification by bacteria were probably not significant sinks for nitrate in comparison with uptake by benthic microbes and dissimilatory reduction to ammonium.     

Growth, carbon, nitrogen and caloric content of Solea senegalensis (Pisces: Soleidae) from egg fertilization to metamorphosis

Eggs and larvae of the Senegal sole, Solea senegalensis Kaup, were reared from fertilization until the end of metamorphosis, which occurs by Day 17 after hatching at 19.5 °C. Changes in energy content and biomass quality were studied in terms of dry weight and of carbon, nitrogen and energy content. S. senegalensis spawned eggs of about 1 mm diameter which hatched 38 h after fertilization. Average dry weight of individual eggs was 46 μg, the chorion accounting for about 18% of total dry weight. Gross energy of recently fertilized sole eggs was approximately 1 J egg⁻¹. From fertilization to hatching, eggs lost 8% of their total energy (chorion not included). After hatching, larvae lost 14% of their initial energy until the start of feeding which occurred about 48 h afterwards. The principal components catabolized during embryogenesis were carbon-rich compounds that decreased by 26%, while nitrogen-rich compounds decreased by only 10% and were practically unaltered from hatching to the start of feeding. Feeding larvae displayed constant growth during the period studied (specific growth rate on a dry weight basis was 0.26 d⁻¹). The relative proportion of carbon and nitrogen content revealed an accumulation of high energy compounds in the days before metamorphosis. By Day 14, the energy content reached values similar to those of recently hatched embryos, but decreased again during metamorphosis. Received: 10 June 1998 / Accepted: 28 January 1999     

Feeding strategy and daily ration of juvenile pink shrimp (Farfantepenaeus duorarum) in a South Florida seagrass bed

 The diet of juvenile pink shrimp (Farfantepenaeus duorarum Burkenroad, previously Penaeus duorarum) from Long Key Bight, Florida Keys, was studied using stomach content examination, pigment measurements, and stable isotope (δ¹³C and δ¹⁵N) analysis. Samples were taken over approximately 24 h on four occasions from December 1997 to June 1998. Juvenile F. duorarum fed nocturnally, the main prey being the seagrass shrimp Thor floridanus (Decapoda: Caridea: Hippolytidae), which accounted for 34% of the stomach content volume. Other common components of the diet were bivalves (mainly Tellina sp.) with 15% volume, calcareous algae (8%), plant detritus (5%), copepods (3%), and seagrass fragments (2%). Pigment concentrations (chlorophyll a plus phaeopigments) in F. duorarum stomachs ranged from 7 to 73 mg l⁻¹ or 40 to 310 ng stomach⁻¹. The exponential gastric evacuation rate was determined experimentally at 1.3 ± 0.5 h⁻¹. Daily rations (in percent body weight) calculated from time series of stomach fullness ranged between 11 and 16% d⁻¹. Total consumption by the population (in wet weight) ranged between 0.05 and 0.3 g m⁻² d⁻¹. Stable isotope measurements confirmed that T. floridanus was the main food source for F. duorarum. δ¹³C-values of whole animals of both species were identical at −10.0 ± 1.6‰ PDB. δ¹⁵N-values of both species were also not significantly different (pooled mean: 5.9 ± 1.7‰). Stomach contents of wild-caught F. duorarum and stomach contents of F. duorarum fed T. floridanus also showed similar stable isotope values. Received: 12 August 1999 / Accepted: 21 March 2000     

Nutrient cycling studies in Carmarthen Bay: phytoplankton production,nitrogen assimilation and regeneration

The results from a series of five cruises (August 1979–January 1981) to examine rates of primary production, nitrogen assimilation and regeneration in Carmarthen Bay, S. Wales, are presented. Chlorophyll, primary production and regeneration were distributed irregularly throughout the bay, with the highest concentrations and rates being found around the shallow periphery. Nitrate was the dominant form of nitrogen assimilated by the phytoplankton, apart from periods of high biomass during the summer when ammonium assumed a more important role. The rates of ammonium regeneration from microheterotrophs were greater than the ammonium demand over much of the year, indicating that the bay was a net exporter of ammonium and was a physically dominated open system. During the summer, the rates of ammonium regeneration and assimilation were similar, indicating efficient recycling of nutrients.     

Post-metamorphic growth of the arms in Ophiophragmus filograneus (Echinodermata: Ophiuroidea) from Tampa Bay,Florida (USA)

Juveniles of the burrowing amphiurid Ophiophragmus filograneus from Tampa Bay, Florida (USA) exhibit a growth pattern unreported in the ophiuroid literature. Two nonadjacent arms grow at a greater rate than the other three arms. This phenomenon might be a developmental adaptation to avoid salinity and temperature fluctuations in surface waters of the shallow-water environment which O. filograneus inhabits. It is proposed that concentration of growth into 2 of the 5 arms permits earlier descent of the disc into the substratum with continued ability to feed on the surface with the arm tips. Larger specimens show a gradual equalization of arm lengths. Changes in growth rates and cropping of the longer arms by breakage and predation might account for such eventual equalization.     

Long-term variation of organochlorine residues and assemblages of epibenthic organisms in a shallow North Florida (USA) estuary

A 4-year study (1972–1976) determined long-term trends of organochlorine residues (DDT, DDE, DDD, PCB's, mirex) and trawl-susceptible organisms in a shallow, river-dominated estuary in North Florida (Apalachicola Bay, USA). Moderate levels of such compounds were found in various species prior to the restricted use of DDT in 1972. A subsequent precipitous decline in organochlorine besidues was attributed to decreased upland usage, major flushing of the river basin in early 1973, and various factors associated with estuarine function. No mirex was found in sediments or aquatic organisms. Apparently, the half-life of organochlorines is relatively short in this bay system. Various statistical methods were used to test the relationships of different physico-chemical and biological parameters. During the 4-year study period, seasonal river flow fluctuations dominated water color, turbidity, salinity, nutrients (NO₃), chlorophyll a, and the temporal succession of fishes in the bay. Certain long-term trends of fish associations were noted; relative dominance of key fish species declined and stabilized while bay-wide species richness and diversity increased with time. Qualitative changes in species representation determined the long-term pattern of community variability. This was consonant with a distinctive fish fauna during the first year of sampling. The bay anchovy Anchoa mitchilli was dominant during 9 of the first 12 monts of the project; this influenced the time-related changes in community indices. Temporally clustered fish associations reflected the importance of river flow in the estuarine environment. Direct correlation of fish distribution with the rapid disappearance of organochlorine compounds was complicated by aperiodic natural phenomena such as storms and river fluctuations. Population and community trends appeared consistent with other studies showing similar patterns of dominance of stress-resistant fish populations and related changes in community parameters. In this case, the relatively predictable annual succession of fish associations allowed an appraisal of key forcing functions. Due to the high level of seasonal and annual biological variability in this estuary, there were some problems in the application of linear statistical models to the data base. Although the long-term trend of relative species representation is useful as an index of stress, new techniques are needed to analyze extensive field data so that functions such as trophic interactions are included in the estimation of causal relationships. There are indications that such effects could be related to the impact of organochlorine compounds on estuarine systems.     

皖南低山丘陵地区流域氮磷径流输出特征

通过对安徽南部宣城地区梅村小流域的定位监测,研究了该小流域地表径流中氮磷输出特征。结果表明:氮输出以NO-3N为主,占氮素输出总量的60%,磷输出以悬浮颗粒结合态磷(PAP)为主,占磷输出总量的92%;各类形态氮素输出量间存在极显著线性正相关关系;PAP随悬浮颗粒输出量的增加而增加,PAP与水溶性磷(DP)输出量间存在着极显著的对数关系;氮磷输出具有明显的季节性变化规律,夏季输出量最大,其次是春季,再次是秋季,冬季最少,这是由降雨量的季节性变化所引起的。而且暴雨对氮磷输出的贡献率极大,因此控制雨季土壤侵蚀是有效控制氮磷通过径流输出的最重要方式。     

Biological aspects ofMonacanthus tomentosus (Monacanthidae) in the seagrass beds of Kotania Bay,West Seram,Moluccas, Indonesia

The present study investigated the growth, mortality, recruitment and food habits ofMonacanthus tomentosus. A total of 1038 specimens were collected by beach seine from the seagrass beds of Kotania Bay (Moluccas, Indonesia) from March 1988 to January 1989. Their length-weight relationship wasW=0.011L ^3.242. Based on the von Bertalanffy formula, the asymptotic length,L , and growth coefficient,K, were determined as 11.79 cm and 0.86, respectively. Total mortality,Z, was low (2.033). Mean length of minimum capturable size (L _c ) was 6.21 cm, and recruitment occurred throughout the year. Food consisted principally of gastropods (21.41%), seagrasses (14.11%), sponges (12.11%), algae (10.82%), amphipods (9.76%) and sedentary polychaetes (9.29%). Pelecypods, opisthobranchs, isopods, copepods, ostracods, foraminiferans, bryozoans, ascidians, nematodes, mollusc eggs and fish eggs were found only in small percentages.     

Effects of kraft-mill effluents on benthic macrophyte assemblages in a shallow-bay system (Apalachee Bay,North Florida,USA)

A 14-month study was carried out to determine the impact of kraft-mill effluents on the offshore benthic macrophyte distribution in a shallow north Florida Bay (USA). A polluted river drainage system was compared to an adjoining (unpolluted) one. The affected area was characterized by elevated levels of color and turbidity. Inshore areas associated with high levels of KME were characterized by severely reduced benthic macrophyte biomass, reduced numbers of species/unit area, and altered species composition when compared to control stations. Areas of chronic impact also had reduced levels of biomass, although the total number of species taken were not significantly different from the controls. In polluted areas, red and brown algae were proportionately more abundant than chlorophytes and spermatophytes. Dominance generally was reduced in areas of the Bay affected by KME. Except for areas of acute impact, there was no significant difference in species diversity (H) between polluted and unpolluted portions of the Bay. There were uniform increases in the total number of species on either side of the affected drainage system. Such areas were considered to be transition zones between the polluted and unpolluted parts of Apalachee Bay. Benthic macrophyte distribution, in terms of biomass and species (community) composition, was considered an important indicator of the impact of KME on the shallow coastal systems in question. The pattern of macrophyte species composition reflected various water-quality parameters. Species normally inhabiting deeper water were found in areas of increased turbidity and color. It was postulated that reduction of normal dominants such as Thalassia testudinum and Halimeda incrassata allowed the colonization of such areas by opportunistic species. This would explain the maintenance of comparable (total) numbers of species in polluted areas and the lack of effect of KME on species diversity even though biomass was severely restricted. These parameters were analogous to the epibenthic fish distribution in the respective drainage areas. Near-shore coastal systems in Apalachee Bay thus were affected by gradients in water quality in addition to natural (seasonal) fluctuations in key physical and chemical parameters. The benthic plant assemblages reflected variations in dominance, the occurrence of opportunistic species, and ecological diversity that resulted in a continuum of disturbance phenomena ranging from sparsely distributed groups in grossly polluted systems to well developed plant assemblages in areas that remain unaffected by KME.     

Dry weight, carbon and nitrogen components of caprellid amphipods (Crustacea) inhabiting the Sargassumyezoense community of Otsuchi Bay, northeastern Japan

Dry weight (DW), carbon (C) and nitrogen (N) contents in four species of caprellids, Caprella danilevskii, C. subinermis, C. penantis R-type, and C. verrucosa, inhabiting Sargassumyezoense (Yamada) in Otsuchi Bay, northeastern Japan, were measured based on live specimens. In all species males tend to grow larger than females in pereonite II length, which is used as an indicator of body length, and have greater dry weight, carbon and nitrogen contents. All three parameters (DW, C and N) had highly significant relationships against pereonite II length. Regressions of female pereonite II length against the three parameters significantly differed from males in the four species. Average percentages of carbon and nitrogen contents of the total dry weight ranged from 30.82 to 32.21% and 5.82 to 6.86%, respectively. The carbon/nitrogen ratio tended to decrease against increasing pereonite II length, e.g., 7.2 to 4.3 in C. danilevskii. Received: 25 November 1996 / Accepted: 31 July 1997     

Larvae of deep-sea fishes (Stomiatoidea) from Biscayne Bay,Florida, USA,and their ecological significance

Twenty stomiatoid fish larvae (3.1 to 21,9 mm) belonging to the genera Bathophilus, Cyclothone, and Stomias were collected in plankton nets from surface waters over depths of 3 to 4 m from Biscayne Bay, Florida, USA. The adults are mesopelagic in the open water of the Florida Current and, therefore, these larvae were presumably advected in parcels of high salinity water into the shallows of Biscayne Bay by winds, tides, or currents. The presence of these larvae suggest that sewage discharged into the Florida Current may be recycling into coastal waters as well as into Biscayne Bay.Scientific Contribution No. 1403, University of Miami, Rosenstiel School of Marine and Atmospheric Science. The support of Environmental Protection Agency Contract FW QA 18050 DIU, Water Quality Office, Division of Water Quality Research is gratefully acknowledged.     

Erratum to: Prey selection by resident common bottlenose dolphins (Tursiops truncatus) in Sarasota Bay, Florida

Prey selection was investigated in wild, resident common bottlenose dolphins, Tursiops truncatus, during the summer months in Sarasota Bay, Florida, USA. Stomach content analyses of 15 dolphins with extensive sighting histories and well-documented distributions were used to determine prey use. Prey availability was assessed by purse seine surveys. We compared the relative abundances of prey available to estimates of prey use at closely matching spatial and temporal scales. G-tests determined that dolphins in this study significantly selected for prey at the species, family, and soniferous/non-soniferous prey levels (G _adj  = 753.98–1,775.93, df = 1–21, p ≤ 0.01). While comprising only 6.3% of the total available prey, soniferous fishes accounted for 51.9% of the total prey consumed. Manly’s standardized forage ratios and 95% Bonferroni confidence intervals determined significant positive selection for soniferous prey and against non-soniferous prey (β^S = 0.9461 vs. β^NS = 0.0539). Dolphins selected against Gerridae, Clupeidae, and Sparidae (β ≤ 0.0014), as well as against all the species within those families (β ≤ 0.0190). It is likely that passive listening for soniferous prey provides an ecological or energetic advantage to cetaceans utilizing this specific foraging technique.     

Complete carbon and nitrogen budgets for the hydromedusaCladonema californicum (Anthomedusa: Cladonemidae)

Complete carbon and nitrogen budgets were constructed for a single cohort of the hydromedusaCladonema californicum Hyman, 1947, collected in 1984 from Santa Catalina Island, California, USA. The budgets accounted for 62 to 84% (average = 74%) of ingested C and 60 to 108% (average = 84%) of ingested N. During most of the medusan life cycle, expenditures for growth exceeded those for metabolism and dissolved organic release (DOR). The gross growth efficiency was lower for C than for N; different conversion rates of C and N are discussed in terms of C:N ratios and budget balances for predator and prey. Growth rates, egg production, and C and N composition ofC. californicum were quite different from those of neritic ctenophores, indicating that gelatinous predators may be a physiologically diverse group.Please address all correspondence and requests for reprints to Dr Costello at his present address: Biology Department, Providence College, Providence, Rhode Island 02918-0001, USA     

Rates of ammonium turnover and the role of amino-acid deamination in seagrass (Zostera capricorni) beds of Moreton Bay,Australia

Samples of sediments from Australian seagrass (Zostera capricorni Aschers.) beds were taken in June to August 1983 (for¹⁵N experiments) and November 1982 to January 1983 (¹⁴N experiments). The ammonium pool turned-over every 0.4 to 0.8 d, as determined with a¹⁵N isotope-dilution technique. The ammonium pool in subtidal bare areas turned-over two to three times more slowly than in adjacent seagrass beds. Gross rates ofin situ ammonium regeneration equalled those of utilization, and ranged from 0.04 to 0.35 mol cm^-3 d^-1, or from 50 to 490 mg N m^-2 d^-1 over the upper 10 cm of the sediment. The potential rate of glycine utilization, measured with a large excess of glycine added to anaerobic incubations, ranged from 0.21 to 0.39mol cm^-3 d^-1, butin situ rates were probably much lower. Between 35 and 65% of added¹⁵N-glycine was deaminated over 12 h, and the remainder was most likely assimilated by microbes. Evidence for the seagrasses taking up glycine was equivocal, owing to the rapid deamination of the amino acid and the likelihood that they assimilated the labelled ammonium produced from the glycine.