Polyvalent cation effects on myo-inositol hexakis dihydrogenphosphate enzymatic dephosphorylation in dairy wastewater

Information is needed on organic polyphosphates such as myo-inositol 1,2,3,5/4,6-hexakis dihydrogenphosphate or phytate (IP6) contribution to the sources and sinks of dissolved phosphorus (PO4-P) in the soil-manure-water system. Effects of Na+, Ca2+, Al3+, and Fe3+ and cation to IP6-P mole ratios on the enzymatic dephosphorylation of IP6 were studied to determine controlling mechanisms of dephosphorylation and persistence in manure. Phytate- and PO4-P were analyzed by high-performance liquid chromatography. Phytate dephosphorylation by Aspergillus ficuum (Reichardt) Henn. phytase EC 3.1.3.8 decreases by 50 +/- 3.6 and 40 +/- 4% at pH 4.5 and 6, respectively, as Ca2+ concentrations increase and cation to IP6-P mole ratios reach 6:6. Polyanionic IP6 has a high affinity for Al3+ and Fe3+ and reductions in dephosphorylation average 27 and 32% at a cation to IP6-P mole ratio of 1:6 for Al3+ and Fe3+, respectively, while reaching more than 99% at a mole ratio of 6:6. A phytase-hydrolyzable phosphorus (PHP) fraction is native to ruminant animal manure and is proportional to total solids (TS) concentration in 1 to 100 g L(-1) suspensions. Added phytase, in effect, increases water-extractable P content of manure and the risk of environmental P dispersion. As the bioavailability and ecological effect of IP6-P appear to be regulated not only by pH-controlled enzyme activity but also by the associated counterions, the differential protective effects of cations influence the accuracy of manure PHP fraction estimates and increase phytate resistance to enzymatic dephosphorylation that may lead to its persistence in manure.     

Organic ligand effects on enzymatic dephosphorylation of myo-inositol hexakis dihydrogenphosphate in dairy wastewater

Animal manure contains partially digested feed fiber and grains where phosphorus (P) is bound in organic compounds that include myo-inositol 1,2,3,5/4,6-hexakis dihydrogenphosphate or phytic acid (IP6). Information is needed on the effects of other (non-IP6) organic ligands (LIGND) on the enzymatic dephosphorylation of IP6, which is a potential source of dissolved orthophosphate P (PO4-P) in the soil-manure-water system. The effects of 1,2-cyclohexane diamino-tetraacetate (CDTA), diethylenetriamine-N,N,N',N',N'-pentaacetate (DTPA), ethylenediamine-N,N,N',N'-tetraacetate (EDTA), oxalate (OXA), and phthalate (PHTH) and LIGND to IP6 molar ratio and charge concentration ratio on IP6 dephosphorylation were studied to determine controlling mechanisms of IP6 persistence in manure. Solution PO4-P concentrations were analyzed by ion chromatography as the phosphomolybdate-ascorbic acid method partly includes IP6-P. Uncomplexed IP6 dephosphorylation by Aspergillus ficuum (Reichardt) Henn. phytase EC 3.1.3.8 at pH 4.5 and 6 is unaffected by the presence of LIGNDs. As the concentrations of Ca2+, Al3+, or Fe3+ increase, dephosphorylation is reduced. Their inhibitory effect lessens in the presence of LIGNDs, in the following order: CDTA = EDTA > DTPA > OXA > or = PHTH. Whether CDTA or EDTA is the most effective LIGND depends upon the acidity of the suspension and LIGND charge concentration, reducing the inhibitory effect of polyvalent counterions to the point of promoting the hydrolysis of a manure phytase-hydrolyzable phosphorus (PHP) fraction that is otherwise unavailable. Therefore, ligand-induced changes increase the mobilization and dephosphorylation of complexed organic P, above and beyond the simple dissolution of inorganic phosphates. An analytical method for potentially bioavailable PHP in animal manure should include a LIGND as extracting reagent. Also, potential LIGNDs in an organic carbon-rich dairy wastewater may increase the release of PHP and environmental dispersion of PO4-P.     

超滤技术在废水处理中的应用

文章对超滤原理、超滤膜种类、国内外超滤技术发展现状等做了较详尽的阐述,同时着重介绍了超滤技术在油田含油废水、石化含油废水、金属加工用乳化废液、纤维加工油剂废水及羊毛精制废水中回收羊毛脂的应用实例。     

膨润土的改性及其在废水处理中的应用

简单介绍了膨润土的结构和基本性质，重点探讨了膨润土的改性方法、改性机理及改性膨润土在废水处理中的应用，指出目前膨润土在废水处理中存在的问题，并展望了其应用前景。     

Reduction of pathogen indicator organisms in dairy wastewater using an ecological treatment system

Ecological treatment systems can provide a sustainable, plant-based alternative to traditional wastewater treatment. One factor essential to the success of these systems is ensuring their ability to reduce coliform concentrations in wastewater. Wastewater is the primary source of fecal contamination in aquatic ecosystems, containing total and fecal coliforms on the order of 10(8)-10(10) and 10(7)-10(9) CFU L(-1), respectively. This study assessed the ability of an ecological treatment system to reduce concentrations of total coliforms and Escherichia coli from dairy wastewater. Low strength wastewater was pumped into the system during July of 2005 and high strength in September 2005. Wastewater passes through a series of anaerobic, aerobic, and clarifier reactors and wetland cells before exiting the system. Regardless of wastewater strength, average total coliform and E. coli concentrations were consistently reduced by at least 99% from influent to effluent, with the majority of the reduction (76%) occurring in the first two reactors. Relationships between internal concentrations of solids and coliforms indicated that increased reduction of solids may further reduce coliform concentrations. Although U.S. Environmental Protection Agency discharge requirements for E. coli were not always met, the substantial reductions achieved indicate that ecological treatment systems have the potential to successfully reduce coliforms in wastewater to meet discharge limits. The results from this study will be used to guide design and management of future ecological treatment systems, so that larger and more consistent coliform reductions can be achieved.     

凤眼莲在污水处理中的应用

叙述了水生植物凤眼莲的生活习性及生物特性，对污水的净化作用，有别于化学处理的各种特点及效果，在我国的发展前景以及使用中应注意的问题等。     

分解协调法在污水处理投资优化计算中的应用

在研究大型的河流综合整治过程中，针对多个点源排放的污染物处理后排入河流具有迁移转化的特点，采用分解协调法对污染治理投资进行协调优化分析，确定各点源各自的最优策略，从而达到整个河流的处理费用最优，为决策部门对河流污染控制方案进行投资具有一定的指导意义。     

Fate of phosphorus in dairy wastewater and poultry litter applied on grassland

Large and repeated manure applications can exceed the P sorption capacity of soil and increase P leaching and losses through subsurface drainage. The objective of this study was to evaluate the fate of P applied with increasing N rates in dairy wastewater or poultry litter on grassland during a 4-yr period. In addition to P recovery in forage, soil-test phosphorus (STP) was monitored at depths to 180 cm in a Darco loamy sand (loamy, siliceous, semiactive, thermic Grossarenic Paleudults) twice annually. A split-plot arrangement of a randomized complete block design comprised four annual N rates (0, 250, 500, and 1000 kg ha(-1)) for each nutrient source on coastal bermudagrass [Cynodon dactylon (L.) Pers.] over-seeded with ryegrass (Lolium multiflorum L. cv. TAM90). Increasing annual rates of N and P in wastewater and poultry litter increased P removal in forage (P = 0.001). At the highest N rate of each nutrient source, less than 13% of applied P was recovered in forage. The highest N rates delivered 8 times more P in wastewater or 15 times more P in poultry litter than was removed in forage harvests during an average year. Compared with controls, annual P rates up to 188 kg ha(-1) in dairy wastewater did not increase STP concentrations at depths below 30 cm. In contrast, the highest annual P rate (590 kg ha(-1)) in poultry litter increased STP above that of controls at depth intervals to 120 cm during the first year of sampling. Increases in STP at depths below 30 cm in the Darco soil were indicative of excessive P rates that could contribute to nonpoint-source pollution in outflows from subsoil through subsurface drainage.     

含有机氟工业废水处理工艺的研究

采用预处理-水解酸化-生物接触氧化相结合的处理工艺处理含氟代有机化合物废水。研究结果表明，水解酸化可将含氟废水的B／C比由0．258提高到0．396％；在一定浓度范围内通过物化预处理及生化处理可以脱除氟代有机化合物中的氟原子，使该有机物成为可供微生物利用的基质。含氟废水经该工艺处理后，出水中BOD5为7．5mg/L,CODcr为75mg/L，氨氮未检出，总磷小于1mg/L，氟离子浓度为8．6mg/L。     

酒精废水治理中二级厌氧的应用与研究

本文对酒精废水治理中二级厌氧的应用进行了分析与研究,提出了高温厌氧和低温厌 氧相结合的二级厌氧新工艺。为酒精废水治理提供了一条稳定可靠、节能低耗的工艺路线。     

Temperature and wetland plant species effects on wastewater treatment and root zone oxidation

Constructed wetlands are widely used for wastewater treatment, but there is little information on processes affecting their performance in cold climates, effects of plants on seasonal performance, or plant selection for cold regions. We evaluated the effects of three plant species on seasonal removal of dissolved organic matter (OM) (measured by chemical oxygen demand and dissolved organic carbon) and root zone oxidation status (measured by redox potential [Eh] and sulfate [SO4(2-)]) in subsurface-flow wetland (SSW) microcosms. A series of 20-d incubations of simulated wastewater was conducted during a 28-mo greenhouse study at temperatures from 4 to 24 degrees C. Presence and species of plants strongly affected seasonal differences in OM removal and root zone oxidation. All plants enhanced OM removal compared with unplanted controls, but plant effects and differences among species were much greater at 4 degrees C, during dormancy, than at 24 degrees C, during the growing season. Low temperatures were associated with decreased OM removal in unplanted controls and broadleaf cattail (Typha latifolia L.) microcosms and with increased removal in beaked sedge (Carex rostrata Stokes) and hardstem bulrush [Schoenoplectus acutus (Muhl. ex Bigelow) A. & D. L?ve var. acutus] microcosms. Differences in OM removal corresponded to species' apparent abilities to increase root zone oxygen supply. Sedge and bulrush significantly raised Eh values and SO4(2-) concentrations, particularly at 4 degrees C. These results add to evidence that SSWs can be effective in cold climates and suggest that plant species selection may be especially important to optimizing SSW performance in cold climates.     

Soil biogeochemical characteristics influenced by alum application in a municipal wastewater treatment wetland

Constructed treatment wetlands are a relatively low-cost alternative used for tertiary treatment of wastewater. Phosphorus (P) removal capacity of these wetlands may decline, however, as P is released from the accrued organic soils. Little research has been done on methods to restore the treatment capacity of aging constructed wetlands. One possibility is the seasonal addition of alum during periods of low productivity and nutrient removal. Our 3-mo mesocosm study investigated the effectiveness of alum in immobilizing P during periods of reduced treatment efficiency, as well as the effects on soil biogeochemistry. Eighteen mesocosms were established, triplicate experimental and control units for Typha sp., Schoenoplectus californicus, and submerged aquatic vegetation (SAV) (Najas guadalupensis dominated). Alum was slowly dripped to the water column of the experimental units at a rate of 0.91 g Al m(-2) d(-1) and water quality parameters were monitored. Soil cores were collected at experiment initiation and completion and sectioned into 0- to 5- and 5- to 10-cm intervals for characterization. The alum floc remained in the 0- to 5-cm surface soil, however, soil pH and microbial parameters were impacted throughout the upper 10 cm with the lowest pH found in the Typha treatment. Plant type did not impact most biogeochemical parameters; however, data were more variable in the SAV mesocosms. Amorphous Al was greater in the surface soil of alum-treated mesocosms, inversely correlated with soil pH and microbial biomass P in both soil layers. Microbial activity was also suppressed in the surface soil of alum-treated mesocosms. This research suggests alum may significantly affect the biogeochemistry of treatment wetlands and needs further investigation.     

CT4-35钻井废水处理剂的研制及应用

CT-35是一种高效的钻井废水处理剂，与其它常用药剂相比，该混凝剂对处理高浓度钻井废水特别是处理难度很大的磺化类废水有明显的优势。介绍了CT4-35研制、生产工艺，用它处理现场钻井废水的情况。现场使用表明：利用CT4-35处理钻井废水，COD的去除率可达85％以上，浊度去除率为100％，处理后出水呈中性（PH6～8），色度小于原水样的1／10，出水清澈透明，并且产生的污泥量少。通过对处理前、后出水的水质分析，证明其中无药剂残留。     

Phosphorus forms in biosolids-amended soils and losses in runoff: effects of wastewater treatment process

Continuous addition of municipal biosolids to soils based on plant nitrogen (N) requirements can cause buildup of soil phosphorus (P) in excess of crop requirements; runoff from these soils can potentially contribute to nonpoint P pollution of surface waters. However, because biosolids are often produced using lime and/or metal salts, the potential for biosolids P to cause runoff P losses can vary with wastewater treatment plant (WWTP) process. This study was conducted to determine the effect of wastewater treatment process on the forms and amounts of P in biosolids, biosolids-amended soils, and in runoff from biosolids-amended soils. We amended two soil types with eight biosolids and a poultry litter (PL) at equal rates of total P (200 kg ha(-1); unamended soils were used as controls. All biosolids and amended soils were analyzed for various types of extractable P, inorganic P fractions, and the degree of P saturation (acid ammonium oxalate method). Amended soils were placed under a simulated rainfall and all runoff was collected and analyzed for dissolved reactive phosphorus (DRP), iron-oxide-coated filter paper strip-extractable phosphorus (FeO-P), and total phosphorus (EPA3050 P). Results showed that biosolids produced with a biological nutrient removal (BNR) process caused the highest increases in extractable soil P and runoff DRP. Alternatively, biosolids produced with iron only consistently had the lowest extractable P and caused the lowest increases in extractable soil P and runoff DRP when added to soils. Differences in soil and biosolids extractable P levels as well as P runoff losses were related to the inorganic P forms of the biosolids.     

Characterization of municipal and dairy wastewaters with 30 quality parameters and potential wastewater treatment by biological trickling filters

This study aimed to characterize raw municipal and dairy wastewaters. The quality of the collected wastewaters was determined by measuring 30 quality parameters, such as phenols, oxidation-reduction potential, oil and grease, total organic carbon (TOC), manganese, total viable count of bacteria (TVC), salinity, and biodegradability. Results were compared with wastewater disposal standards. This study also aimed to describe the potential application and performance of biological trickling filters (BTFs) in municipal and dairy wastewater treatment. Detailed reviews of trickling filter models were explained. Results revealed that two wastewater samples exceeded standard limits. The raw municipal and dairy wastewaters need treatment prior to disposal to the natural environment. Additionally, BTFs are efficient municipal and dairy wastewater treatments.     

生物工程在废水净化中的应用及发展趋势

水污染问题是环境保护领域中重要的问题之一,如何高效的利用生物工程技术来解决这一问题已成为环保界普遍重视的问题。本文总结了近年来生物工程在废水净化中的应用现状,并对当前废水净化中生物工程的发展趋势进行了探讨。     

Determination of steroidal estrogens in flushed dairy manure wastewater by gas chromatography-mass spectrometry

There is a critical need to accurately measure the concentrations of natural steroidal estrogens in flushed dairy manure wastewater (FDMW) to assess any potential risk of waterway contamination resulting from land application. Estrogens are a concern because low concentrations (10-100 ng L-1) in water can adversely affect aquatic vertebrate species such as fish, turtles, and frogs by disrupting the normal function of their endocrine systems. The objective of this study was to develop a sample preparation method that permits the quantification of four natural steroidal estrogens (17alpha-estradiol, 17beta-estradiol, estrone, and estriol) in FDMW by gas chromatography-mass spectrometry (GC-MS). Solid-phase extraction with graphitized carbon black was used for the bulk extraction of estrogens from FDMW and additional sample purification was accomplished with C-18. The sample preparation method allowed estrogens to be detected accurately by GC-MS in FDMW. Spiked recovery experiments indicated that the method is satisfactory for measuring the estrogens of interest in FDMW with average recovery of >90%. As expected in FDMW, characterization of the estrogen profile revealed a large abundance of 17alpha-estradiol relative to 17beta-estradiol and estrone. Estriol was not detected in FDMW. The methodology developed in this research helps provide an analytical foundation for the quantification of steroidal estrogens in FDMW by GC-MS.     

Biological treatment of tannery wastewater in the presence of chromium

Experiments were carried out to determine the feasibility of treating tannery wastewater containing chromium, an inhibiting compound, with sequencing batch reactors (SBR). The maximum chromium concentration tolerated by microorganisms was determined through aerobic and anoxic batch experiments, and the biomass inhibition process was analyzed in a lab scale reactor at increasing chromium concentrations. The results obtained, in batch experiments and in the SBR reactor, have demonstrated that chromium addition had less influence on the denitrification bacteria than on the nitrification bacteria. In addition, it was observed that nitrification and denitrification rates, at the same chromium concentration, were higher in the SBR reactor than in batch experiments with unacclimated biomass. Experimental results confirm that sequencing batch reactors are able to produce a more resistant biomass, which acclimates quickly to inhibiting conditions. A large amount of chromium was found in the sludge from the reactor, while the effluent was devoid of the inhibiting metal.     

Para-chlorophenol containing synthetic wastewater treatment in an activated sludge unit: effects of hydraulic residence time

Due to the toxic nature of chlorophenol compounds present in some chemical industry effluents, biological treatment of such wastewaters is usually realized with low treatment efficiencies. Para-chlorophenol (4-chlorophenol, 4-CP) containing synthetic wastewater was treated in an activated sludge unit at different hydraulic residence times (HRT) varying between 5 and 30 h while the feed COD (2500 mg l(-1)), 4-CP (500 mg l(-1)) and sludge age (SRT, 10 days) were constant. Effects of HRT variations on COD, 4-CP, toxicity removals and on settling characteristics of the sludge were investigated. Percent COD removals increased and the effluent COD concentrations decreased when HRT increased from 5 to 15 h and remained almost constant for larger HRT levels. Nearly, 91% COD and 99% 4-CP removals were obtained at HRT levels above 15 h. Because of the highly concentrated microbial population at HRT levels of above 15 h, low effluent (reactor) 4-CP concentrations and almost complete toxicity removals were obtained. High biomass concentrations obtained at HRT levels above 15 h were due to low 4-CP contents in the aeration tank yielding negligible inhibition effects and low maintenance requirements. The sludge volume index (SVI) decreased with increasing HRT up to 15 h due to high biomass concentrations at high HRT levels resulting in well settling sludge with low SVI values. Hydraulic residence times above 15 h resulted in more than 90% COD and complete 4-CP and toxicity removals along with well settling sludge.     

Comparison of three enzyme immunoassays for measuring 17beta-estradiol in flushed dairy manure wastewater

Natural steroidal estrogens are an environmental concern because low nanogram per liter concentrations in water can adversely affect aquatic vertebrate species by disrupting the normal function of their endocrine systems. There is a critical need to accurately measure estrogens in dairy wastes, a potential source of estrogens such as 17beta-estradiol, to assess the risk of estrogen contamination of agricultural drainage waters resulting from land application. Commercially available enzyme immunoassay (EIA) kits have been used for measuring 17beta-estradiol in livestock manure, but it is not known if different EIAs provide similar results. We compared three EIAs by measuring 17beta-estradiol in two samples of flushed dairy manure wastewater (FDMW). The measured concentrations of 17beta-estradiol in FDMW differed according to the immunoassay used. The differences were attributed to a matrix interference associated with coextracted humic substances. Future research should develop methods that enable routine measurement of 17beta-estradiol in livestock wastes by more conclusive analytical techniques such as gas chromatography-mass spectrometry.