Use of ATP Readings to Predict a Successful Hygiene Intervention in the Workplace to Reduce the Spread of Viruses on Fomites

The purpose of this study was to validate the use of adenosine triphosphate (ATP) for evaluating hygiene intervention effectiveness in reducing viral dissemination in an office environment. The bacterial virus MS-2 was used to evaluate two scenarios, one where the hand of an individual was contaminated and another where a fomite was contaminated. MS-2 was selected as a model because its shape and size are similar to many human pathogenic viruses. Two separate experiments were conducted, one in which the entrance door push plate was inoculated and the other in which the hand of one selected employee was inoculated. In both scenarios, 54 selected surfaces in the office were tested to assess the dissemination of the virus within the office. Associated surface contamination was also measured employing an ATP meter. More than half of the tested hands and surfaces in the office were contaminated with MS-2 within 4 h. Next, an intervention was conducted, and each scenario was repeated. Half of the participating employees were provided hand sanitizer, facial tissues, and disinfecting wipes, and were instructed in their use. A significant (p < 0.05) reduction was observed in the number of surfaces contaminated with virus. This reduction in viral spread was evident from the results of both viral culture and the surface ATP measurements, although there was no direct correlation between ATP measurements with respect to viral concentration. Although ATP does not measure viruses, these results demonstrate that ATP measurements could be useful for evaluating the effectiveness of hygiene interventions aimed at preventing viral spread in the workplace.     

Use of Hygiene Protocols to Control the Spread of Viruses in a Hotel

The goals of this study were to observe the spread of viruses in a hotel setting and to assess the effectiveness of a hygiene intervention in reducing their spread. Selected fomites in one hotel room were inoculated with bacteriophage ?x-174, and fomites in a conference center within the same hotel were inoculated using bacteriophage MS2. Cleaning of the contaminated room resulted in the spread of viruses to other rooms by the housekeeping staff. Furthermore, viruses were transferred by hotel guests to the conference center and a communal kitchen area. Additionally, conference attendees transferred viruses from the conference center to their hotel rooms and a communal kitchen area. This study demonstrated how viruses can be spread throughout a hotel setting by both housekeepers and guests. A hygiene intervention, which included providing hand hygiene products and facial tissues to the guests and disinfecting solutions with disposable wipes to the housekeeping staff, was successful in reducing the spread of viruses between the hotel guest rooms and conference center. The hygiene intervention resulted in significantly reduced transfer of the ?x-174 between the contaminated hotel room and other hotel rooms, communal areas, and the conference center (p = 0.02).     

提高医务人员手部卫生依从性干预措施的研究进展

提高医务人员手部卫生依从性一直是个重要的研究课题,笔者参考国内外相关文献,综述了提高医务人员手部卫生依从性干预措施的研究进展,提出今后要重视采取多方位综合措施的研究。     

太极拳运动的传播方式研究

本文以太极拳运动的简介与起源为切入点,展现出图文并茂、灵活廉价、寓学于乐的太极拳运动,然后重点分析了该运动的三种传播方式,进而阐述了报纸、杂志、广播、电视以及互联网等现代传媒是传播太极拳运动的最有效途径。因此,研究分析太极拳运动的传播途径具有重要意义,并可以一定程度上为其他武术拳种的传播提供有关的理论依据,并促进其良好发展。     

Comparison of the Activity of Alcohol-Based Handrubs Against Human Noroviruses Using the Fingerpad Method and Quantitative Real-Time PCR

Noroviruses (NoV) are the most common cause of acute nonbacterial gastroenteritis in the United States, and human hands play an important role in their transmission. Little is known about the efficacy of hand hygiene agents against these highly infectious pathogens. We investigated the activity of seven commercially available hand hygiene products against human noroviruses by in vivo fingerpad tests. The in vivo activity of alcohol-based handrubs ranged from 0.10 to 3.74 log reduction and was not solely dependent on alcohol concentration. A handrub (VF481) based on 70% ethanol and a blend of other skin care ingredients reduced Norwalk virus (NV) by 3.74 log in 15 s and provided significantly greater NV reduction than all the other products tested (P < 0.001). Furthermore, VF481 was the most effective product tested against the NoV genogroup II strains Snow Mountain virus (GII.2) and a GII.4 strain. These results demonstrate that alcohol by itself is not effective against NoV, but effective formulation of alcohol-based handrubs can achieve significant reduction of norovirus RNA on fingers.     

Survival of Respiratory Viruses on Fresh Produce

In addition to enteric viruses of fecal origin, emerging zoonotic viruses such as respiratory coronaviruses and influenza viruses may potentially be transmitted via contaminated foods. The goal of this study was to determine the recovery efficiencies and the survival of two respiratory viruses, namely, adenovirus 2 (Ad2) and coronavirus 229E (CoV229E), on fresh produce in comparison to the enteric poliovirus 1 (PV1). Adenovirus was recovered with efficiencies of 56.5, 31.8, and 34.8 % from lettuce, strawberries, and raspberries, respectively. Coronavirus was recovered from lettuce with an efficiency of 19.6 % yet could not be recovered from strawberries. Poliovirus was recovered with efficiencies of 76.7 % from lettuce, but only 0.06 % from strawberries. For comparison purposes, the survival of Ad2, CoV229E, and PV1 was determined for periods up to 10 days on produce. The enteric PV1 survived better than both respiratory viruses on lettuce and strawberries, with only ≤1.03 log₁₀ reductions after 10 days of storage at 4 °C compared to CoV229E not being recovered after 4 days on lettuce and reductions of 1.97 log₁₀ and 2.38 log₁₀ of Ad2 on lettuce and strawberries, respectively, after 10 days. Nevertheless, these respiratory viruses were able to survive for at least several days on produce. There is therefore the potential for transfer to the hands and subsequently to the mucosa via rubbing the eyes or nose. In addition, some respiratory coronaviruses (e.g., severe acute respiratory syndrome coronavirus) and adenoviruses are also capable of replication in the gut and there is thus some potential for acquisition through the consumption of contaminated produce.     

Issues Concerning Survival of Viruses on Surfaces

Viruses are the causative agents of an estimated 60% of human infections worldwide. The most common viral illnesses are produced by enteric and respiratory viruses. Transmission of these viruses from an infected person or animal to a new host can occur via several routes. Existing studies strongly suggest that contaminated fomites or surfaces play an important role in the spreading of viral diseases. The potential of viral spreading via contaminated surfaces depends particularly on the ability of the virus to maintain infectivity whilst it is in the environment. This is affected by a combination of biological, physical and chemical factors. This review summarises current knowledge about the influence of environmental factors on the survival and spread of viruses via contaminated surfaces.     

A 1-Year Study on the Detection of Human Enteric Viruses in New Caledonia

Human enteric viruses occur in high concentrations in wastewater and can contaminate receiving environmental waters. Due to the lack of data on the prevalence of enteric viruses in New Caledonia, the presence and the concentrations of enteric viruses in wastewater and seawater were determined. Untreated wastewater and seawater samples were collected monthly for 1 year from a wastewater treatment plant (WWTP) and from the WWTP’s outlet, located directly on a popular recreational beach. Samples were tested for norovirus genogroups I and II (NoV GI and GII), astroviruses (AsV), sapoviruses (SaV), enteroviruses (EV), hepatitis A viruses (HAV), rotaviruses (RoV), human adenoviruses (HAdV) and human polyomaviruses (HPyV). To support these data, faecal samples from cases of gastroenteritis were tested for the first time for NoV and detected in the population. NoV GI, NoV GII, EV, SaV, HAdV and HPyV were detected in all wastewaters, RoV in 75 % and AsV in 67 %. HAV were not detected in wastewater. Overall, 92 % of seawater samples were positive for at least one virus. HPyV were detected most frequently in 92 % of samples and at concentrations up to 7.7 × 10³ genome copies/L. NoV GI, NoV GII, EV, SaV, RoV and HAdV were found in 33, 66, 41, 33, 16 and 66 % of seawater samples, respectively. AsV were not detected in seawater. This study reports for the first time the presence of NoV and other enteric viruses in New Caledonia and highlights the year-round presence of enteric viruses in the seawater of a popular beach.     

Efficacy of a Levulinic Acid Plus Sodium Dodecyl Sulfate (SDS)-Based Sanitizer on Inactivation of Influenza A Virus on Eggshells

Influenza A virus poses a major public health concern and is associated with annual epidemics and occasional pandemics. Influenza A H3N2 viruses, which are an important cause of human influenza, can infect birds and mammals. Contaminated undercooked poultry products including eggs with avian influenza virus constitute a possible risk of transmission to humans. In this study, a novel levulinic acid plus sodium dodecyl sulfate (SDS) sanitizer was evaluated for eggshell decontamination. Influenza A H3N2 virus-inoculated chicken eggshells were treated with a 5 % levulinic acid plus 2 % SDS, 2 % levulinic acid plus 1 % SDS, and 0.5 % levulinic acid plus 0.5 % SDS liquid solution for 1 min. Log reductions of viable viruses were observed by plaque assay. The 5 % levulinic acid plus 2 % SDS sanitizer provided the greatest level of influenza A H3N2 virus inactivation (2.23 log PFU), and differences in virus inactivation were observed for the various levulinic acid plus SDS concentrations tested (P ≤ 0.05). To the best of our knowledge, this is the first study demonstrating influenza A H3N2 virus inactivation on eggshells using a novel levulinic acid plus SDS sanitizer. The sanitizer may be useful for reducing egg contamination and preventing the spread of avian influenza virus to humans.     

加强IS014000环境管理体系在医院推广

简介了1S014000环境管理体系,并论述了IS014001标准在医院的实施步骤,进一步详细分析了环境管理体系对医院安全处置医疗废弃物,加强环境保护工作的推动作用。     

推动国语传播的民国白话文教科书

清末民初的白话文运动取得了白话文取代文言文,成为正式书面语的成果。在这一过程中,民国白话文教科书功不可没。它不仅从制度上确立了白话这一话语形式在教育领域的合法地位,更重要的在于奠定了白话的话语霸权地位,作为传播现代白话文的重要工具,它以自身独特的优势,不仅加快了国语的传播速度,扩大了国语的传播范围,而且也逐步规范了国语。     

Detection of Pathogenic Viruses in the Ambient Air in Seoul,Korea

The possible transport of pathogenic microorganisms during Asian dust events could be an important concern for health workers; however, this is still uncertain owing to a lack of supporting evidence. The present study aimed to investigate the presence of pathogenic microorganisms in air samples collected during the Asian and non-Asian dust periods. Between March and September 2016, air samples were collected at three weather observation stations in Seoul using a high-volume air sampler. Multiplex PCR was performed using the Allplex? respiratory and gastrointestinal panel assay kits to detect 46 microorganisms. RT-PCR was performed for klassevirus, Aichivirus, and human parechovirus (HPeV) detection. In total, 71 air samples were collected during the Asian (8 samples) and non-Asian (63 samples) dust events. During an Asian dust event, only one human rhinovirus (HRV)-positive air sample was collected on April 23. During the non-Asian dust period, HRV, HPeV, norovirus (NoV), enteroaggregative Escherichia coli (EAEC), enterotoxigenic E. coli (ETEC), and Blastocystis hominis were detected in four, two, one, one, one, and one air samples, respectively. Pathogenic viruses were mostly detected in ambient air samples during the non-Asian dust period, which suggests a possible air-borne transmission of viral pathogens; however, the role of Asian dust in epidemics caused by pathogenic viruses is unclear.     

微生物对装备的影响

微生物作为自然环境中的组成物质,在大气、水体、土壤等环境中广泛存在和分布;而装备在运输、存储和服役的各个阶段,都与这些环境有密切关系,所以微生物与装备不可避免地会发生接触。一般情况下,各类微生物都有可能直接或间接地侵蚀破坏装备及材料。介绍了微生物对装备及其构件材料的影响现状,着重阐述了微生物对装备及材料的腐蚀影响。     

解决压载水微生物入侵性传播研究的国际动态

船舶压载水排放造成了地理性隔离水体间的生物入侵，是有害微生物传播的最主要途径。有害水生生物和病原体通过压载水传播，严重威胁侵入国的经济、环境及人类健康，已引起世界范围的广泛关注，许多国家都已开始着手调查外来生物入侵现状，研究管理措施、治理对策及方法。文中对此作一综述。     

Identification of Enteric Viruses in Foods from Mexico City

Foodborne viruses are a common and, probably, the most under-recognized cause of outbreaks of gastroenteritis. Among the main foods involved in the transmission of human enteric viruses are mollusks, and fruits and vegetables irrigated with wastewater and/or washed with non-potable water or contaminated by contact with surfaces or hands of the infected personnel during its preparation. In this study, 134 food samples were analyzed for the detection of Norovirus, Rotavirus, and Hepatitis A virus (HAV) by amplification of conserved regions of these viruses. From the 134 analyzed samples, 14 were positive for HAV, 6 for Norovirus, and 11 for Rotavirus. This is the first report in Mexico where emphasis is given to the presence of HAV and Norovirus on perishable foods and food from fisheries, as well as Rotavirus on frozen vegetables, confirming the role of vegetables and bivalve mollusks as transmitting vehicles of enteric viruses.     

从人文关怀的角度谈对地震灾区儿童的心理干预

近年来地质灾害频发,从汶川地震到玉树地震再到雅安的芦山地震,每一次地震都会给儿童造成不同程度的心理创伤,并在身心、认知和精神上表现有一定的创伤应激障碍,因此,需要针对地震灾区儿童各年龄阶段心理创伤的反应特点,提出具体的护理措施,着重分析人文关怀在灾区儿童心理干预中的运用,以及人文关怀对灾区儿童心理重建的重要意义。     

奥里油漂移扩散特性的实验及数值模拟研究

奥里油是我国目前大量进口的一种新型燃油,由大型油轮从委内瑞拉海运至中国湛江港口。文章以其在运输过程中可能发生的溢油事故为研究对象,对奥里油在水体中的漂移扩散过程进行了数值模拟以及相关实验。考虑到溢油扩散主要是受油品本身的性质(油类、油量、密度、黏度等)以及水流、风速的影响,文章尝试采用计算流体力学FLUENT软件中的VOF模型进行扩散模拟,以水槽溢油实验为模型建立基础,对三维无风速情况下进行气-油-水三相模拟,根据模拟得到的溢油扩散相图与实验所观察的溢油扩散轨迹比较可得出:FLUENT软件中的VOF模型能很好的模拟奥里油溢油扩散运动的情况,其结果与实验观察结果非常吻合。     

组织成员流动性对组织学习中知识传播的影响

非正式群体的个体是知识传播的有效途径,知识通过个体传播到正式组织形成组织知识。在分析并提出必要假设基础上,构建不同条件下知识传播模型,分析组织成员流动性对知识传播的高峰期和组织的知识存量的影响,为组织管理者促进学习型组织形成提供理论依据。     

土壤石油生物降解影响因子正交实验分析

石油污染物进入土壤之后，多种因素同时制约着污染物的生物降解，为了定量化各因素对彼此的影响，探求促进正向作用，抑制负向作用的途径，为各因素制定适宜的施用时间顺序以及施用量，以最大限度地加快降解速度，笔者设计了本文中的复合生物降解实验，结果表明，使用多因素复合处理方法可以在土壤石油处理中收到很好的效果，在土壤石油含量为64g/kg水平下，三周内的降解率可以高达48％，在实验初期，表面活性剂和氧化剂的投加是影响降解速率的重要因素，它们的最优水平均为各受试水平中的最大值，到了实验的中后期，污染强度上升为降解的首要影响因子，并且在受试样品范围内，污染度越大降解率越高。