社会可持续性与合作学习

社会层面在农业一食品系统的可持续发展中占中心地位.如果农民对他们的现状不满意或者丧失了继续从事农业的动力,那么现有的很多环境目标将不可能实现.在1997～2003年间,对社会可持续性、认可在农业系统中的重要性、行为者之间逐渐增加的合作的潜在作用进行了一些案例研究.研究的主要假定是,不断增加的社会认可是可持续社会环境的基础.我们的研究结果表明,现在许多农民认为他们社会地位低下.他们认为自己缺乏对决策的控制权,因而制约了他们继续从事农业生产的能力.公众对他们的印象和政治决策的制定过程都显得缺乏对农民技巧和知识的尊敬.但是,行为者之间日增曾的合作被认为是重大的进步,因为它创造了一个强大的关系网,给农民以一个较强势的身份.我们发现,在社会生态可持续性的合作学习和决策制定过程中最重要的是,农民需要官方和其它组织的支持、提供帮助,这样该过程才会更容易完成.     

Interlaboratory evaluation of endotoxin analyses in agricultural dusts--comparison of LAL assay and mass spectrometry

Endotoxin exposure is associated with wheeze and asthma morbidity, while early life exposure may reduce risk of allergy and asthma. Unfortunately, it is difficult to compare endotoxin results from different laboratories and environments. We undertook this study to determine if lipopolysaccharide (LPS) extraction efficiency could account for differences among laboratories. We generated and collected aerosols from chicken and swine barns, and corn processing. We randomly allocated side-by-side filter samples to five laboratories for Limulus assay of endotoxin. Lyophilized aliquots of filter extracts were analyzed for 3-hydroxy fatty acids (3-OHFAs) as a marker of LPS using gas chromatography-mass spectrometry. There were significant differences in endotoxin assay and GC-MS (LPS) results between laboratories for all dust types (p < 0.01). Patterns of differences between labs varied by dust type. Relationships between assay and GC/MS results also depended on dust type. The percentages of individual 3-OHFA chain lengths varied across labs (p < 0.0001) suggesting that each lab recovered a different fraction of the LPS available. The presence of large amounts of particle associated LPS and absence of a freezing thawing cycle were associated with lower correlations between LPS and bioactivity, consistent with an absence of Limulus response to cell-bound endotoxin. These data suggest that extraction methods affect endotoxin measurements. The LAL methods may be most suitable when comparing exposures within similar environments; GC-MS offers additional information helpful in optimizing sample treatment and extraction. GC-MS may be of use when comparing across heterogeneous environments and should be considered for inclusion in future studies of human health outcomes.     

北欧湖泊鱼类状况调查:酸化、富营养化和放养活动对现有鱼类品种组成的影响

1995～1997年间对挪威、瑞典和芬兰的3821个湖泊的鱼类种群状况做了评估.采用分层随机抽样从芬诺斯堪的亚(芬兰、挪威、瑞典、丹麦的总称)所有面积≥0.04km2的126482个湖泊中选择调查湖泊.分析了湖泊的水化学,湖内鱼类状况则从邮件调查问卷中获得.鱼类种群损失发生最为频繁的地区在挪威南部酸化最严重的地区,而在芬诺斯堪的亚东部最少.根据调查问卷,鳟(Salmotrutta)、拟鲤(Rutilus rutilus)、北极红点鲑(Salvelinus alpinus)和鲈鱼(Percafluviatilis)鱼群损失的数量估计超过10000条.这些品种的鱼群潜在地受低碱性湖水影响的数量超过11000条.大约有3300个湖泊显示出总磷高于25μg/L,品种以鲤科鱼为主,这在芬诺斯堪的亚东部、芬兰西南最为明显.这次调查并未显示出鱼类品种由于富营养化而灭绝.1/3的湖泊人工放养了至少一个新的鱼类品种,最常放养的品种有鳟、白鲑(Coregonus lavaretus s.1.)、北极红点鲑、虹鳟(Oncorhynchusmykiss)、梭鲈(Stizostedion lucioperca)、茴鱼(Thymallus thymallus)、狗鱼(Esox lucius)、欧鳊(Abramis brama)、丁 (Tinca tinca)和欧 (Phoxinus phoxinus).在芬诺斯堪的亚湖泊,这些品种的人工放养量估计超过52000群.因此,北欧湖泊现有鱼类品种的数量最近受放养的影响超过了因环境变化如酸化等而损失造成的影响.     

S-100 protein and neuron-specific enolase in amniotic fluid as markers of abdominal wall and neural tube defects in the fetus

The aim of this study was to determine whether there is increased leakage of neuron-specific enolase (NSE) and S-100 protein into amniotic fluid in pregnancies with neural tube defects, since both these proteins are produced by neural tissue, and to compare the value of these substances for detecting such defects with that of the more conventional techniques of alpha-fetoprotein (AFP) and acetylcholinesterase (AChE) gel electrophoresis. Amniotic samples from 25 mid-pregnancies (15–17 weeks' gestation) with neural tube defects (14 with open spina bifida and 11 with anencephaly) and from seven mid-pregnancies with abdominal wall defects were compared with a control material consisting of 80 amniotic fluid samples from 80 consecutive mid-pregnancy amniocenteses, with normal karyotypes and AFP concentrations. All of the above cases of abnormalities were primarily detected through increased AFP levels in the amniotic fluid. Amniotic fluid samples from 13 pregnancies with fetuses with autosomal chromosomal abnormalities and seven amniotic fluid samples contaminated with blood were also included in the investigation. It is concluded from the results that the conventional AFP assay combined with AChE gel electrophoresis is the best method for screening amniotic fluid for neural tube defects and defects of the abdominal wall. Neither NSE nor S-100 assay alone proved to be superior for the detection of these cases in mid-trimester amniotic fluid. The S-100 assay, however, could give additional information in cases where AChE gel electrophoresis is not decisive; for example, in samples contaminated with blood.     

A new neosuchian with Asian affinities from the Jurassic of northeastern Brazil

Phylogenetic relationships near the origin of extant crocodylians are weakly supported, and this lack of resolution makes for poor estimates of taxonomic and morphological diversity. Previously known taxa are found throughout the Cretaceous in Laurasia and at a few sites from Brazil, Australia, and northern Africa. Here, we report Batrachomimus pastosbonensis gen. et sp. nov. from the Late Jurassic of northeastern Brazil, which is deeply nested within Neosuchia and associated to the Cretaceous Asian paralligatorids. The new taxon predates all other members of Paralligatoridae and its immediate sister group (including Eusuchia) by 30 million years. A nearly complete skull, osteoderms, and limb bones were recovered, and autapomorphies of B. pastosbonensis include a scalloped lateral margin of the rostrum in dorsal view, unsculpted alveolar margin at the caudalmost portion of the maxilla, blunt lateral prongs on the jugal at the base of the postorbital bar, hourglass shaped choanae, and pterygoid choanal septum extended between the palatal shelves of the palatines. The crocodyloid-like skull proportions and the slender, conical teeth suggest an amphibious and piscivorous life history to this 1 m long animal.

     

The need for a systems theory approach to road safety

In other hazardous complex socio-technical systems in society, e.g. nuclear power and aviation, systems-theoretical assumptions are considered a promising way to better understand and manage safety. In this paper, two fundamental approaches to road safety were assessed in the light of a systems theory approach. One approach, is based on a premise where individual road-users are solely responsible when crashes occur. In that case countermeasures are aimed at altering the behavior of the road-user in order to adapt him/her to the road transport system. The other approach, the so-called zero-tolerance position, or Vision Zero approach, to road safety is built around two axioms; the system must be adapted to the psychological and physical conditions and limitations of the human being and the responsibility for road safety must be shared between the road-users and the designers and professional operators of the system. It was found that the most important determinants of systems theory are basically not present in the road-user approach. However, even if the Vision Zero approach clearly takes step towards systems theory, it does leave room for articulating even more features of systems theory.     

Stem cell transplantation before birth – a realistic option for treatment of osteogenesis imperfecta?

Osteogenesis imperfecta (OI) is characterized by severe bone deformities, growth retardation and bones that break easily, often from little or no apparent cause. OI is a genetic disorder primarily with defective type I collagen with a wide spectrum of clinical expression. In the more severe cases, it can be diagnosed before birth. Transplantation of mesenchymal stem cells (MSC) has the potential to improve the bone structure and stability, growth and fracture healing. Prenatal and postnatal cell transplantation has been investigated in preclinical and clinical studies of OI and suggests that this procedure is safe and has positive effects. Cell transplantation resulted in improved linear growth, mobility and reduced fracture incidence. However, the effect is transient and for this reason re-transplantation may be needed. So far there is limited experience in this area, and proper studies are required to accurately determine if MSC transplantation is of clinical benefit in the treatment of OI. In this review, we summarize what is currently known in this field. © 2015 John Wiley & Sons, Ltd.     

Sequentielle Schwermetallextraktion aus Staubniederschl?gen und Stra?ensedimenten Teil I: Sequentielle Schwermetallextraktion von Mikroproben

Für die Anwendung der sequentiellen Schwermetallextraktion auf Proben, die nur im Milligramm-Bereich erhalten werden k?nnen (Mikroproben, wie z. B. Staubniederschl?ge), wurde die Methode vonZeien &Brümmer (1989) weiterentwickelt. Der Extraktionsma?stab wurde um den Faktor 1∶100 verringert und die Reproduzierbarkeit des Verfahrens mit variablen Probenmengen um 20mg und einem Extraktionsvolumen von 500 μl mit Hilfe von Standardb?den nachgewiesen. M?gliche Einflüsse variabler Extraktionsverh?ltnisse (Verh?ltnis von Probenmenge zu Extraktionsvolumen) sowie eine aufwendige Vorbehandlung von Staubproben, die nach der Bergerhoff-Methode (VDI 2119/2, 1972) gesammelt wurden, lagen bei diesen Untersuchungen weitgehend im Variationsbereich des Verfahrens. Die sequentielle Extraktion kann folglich zur Untersuchung der Mobilisierbarkeit von Schwermetallen auch aus Mikroproben mit variablen Probenmengen eingesetzt werden. Mit Hilfe dieses Verfahrens wurden die Mobilisierbarkeiten von Zink, Kupfer und Blei der Schluff- und Tonfraktion von Stra?ensedimenten mit Staubniederschl?gen an zwei urbanen Standorten mit unterschiedlicher, verkehrsbedingter Schwermetallbelastung verglichen. Diese Untersuchungen werden in Teil 2 (“Sequentielle Schwermetallextraktion von st?dtischen St?uben”) vorgestellt.