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1.
Stin OC Carnahan A Singh R Powell J Furuno JP Dorsey A Silbergeld E Williams HN Morris JG 《Environmental monitoring and assessment》2003,81(1-3):327-336
Molecular methods, including DNA probes, were used to identify and enumerate pathogenic Vibrio species in the Chesapeake Bay; our data indicated that Vibrio vulnificus exhibits seasonal fluctuations in number. Our work included a characterization of total microbial communities from the Bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. To identify members of the microbial community, we amplified the 16S rDNA gene from community DNA isolated from a biofilm sample collected from the Chesapeake Bay in February, 2000. The resultant 75 sequences were 95% or more similar to 7 species including two recently described Shewanella species, baltica and frigidimarina, that have not been previously isolated from the Chesapeake. When the genera of bacteria from biofilm after culturing are compared to those detected by subcloning amplified 16S fragments from community DNA, the cultured sample exhibited a strong bias. In oysters collected in February, the most common bacteria were previously unknown. Based on our 16S findings, we are developing microarrays to detect these and other microbial species in these estuarine communities. The microarrays will detect each species using four distinct loci, with the multiple loci serving as an internal control. The accuracy of the microarray will be measured using sentinel species such as Aeromonas species, Escherichia coli, and Vibrio vulnificus. Using microarrays, it should be possible to determine the annual fluctuations of bacterial species (culturable and non-culturable, pathogenic and non-pathogenic). The data may be applied to understanding patterns of environmental change; assessing the health of the Bay; and evaluating the risk of human illness associated with exposure to and ingestion of water and shellfish. 相似文献
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We examined the principal effects of different information network topologies for local adaptive management of natural resources.
We used computerized agents with adaptive decision algorithms with the following three fundamental constraints: (1) Complete
understanding of the processes maintaining the natural resource can never be achieved, (2) agents can only learn by experimentation
and information sharing, and (3) memory is limited. The agents were given the task to manage a system that had two states:
one that provided high utility returns (desired) and one that provided low returns (undesired). In addition, the threshold
between the states was close to the optimal return of the desired state. We found that networks of low to moderate link densities
significantly increased the resilience of the utility returns. Networks of high link densities contributed to highly synchronized
behavior among the agents, which caused occasional large-scale ecological crises between periods of stable and high utility
returns. A constructed network involving a small set of experimenting agents was capable of combining high utility returns
with high resilience, conforming to theories underlying the concept of adaptive comanagement. We conclude that (1) the ability
to manage for resilience (i.e., to stay clear of the threshold leading to the undesired state as well as the ability to re-enter
the desired state following a collapse) resides in the network structure and (2) in a coupled social–ecological system, the
systemwide state transition occurs not because the ecological system flips into the undesired state, but because managers
lose their capacity to reorganize back to the desired state.
An erratum to this article can be found at . 相似文献
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Evelien?M.?de OldeEmail author Henrik?Moller Fleur?Marchand Richard?W.?McDowell Catriona?J.?MacLeod Marion?Sautier Stephan?Halloy Andrew?Barber Jayson?Benge Christian?Bockstaller Eddie?A.?M.?Bokkers Imke?J.?M.?de Boer Katharine?A.?Legun Isabelle?Le Quellec Charles?Merfield Frank?W.?Oudshoorn John?Reid Christian?Schader Erika?Szymanski Claus?A.?G.?S?rensen Jay?Whitehead Jon?Manhire 《Environment, Development and Sustainability》2017,19(4):1327-1342
Sustainability indicators are well recognized for their potential to assess and monitor sustainable development of agricultural systems. A large number of indicators are proposed in various sustainability assessment frameworks, which raises concerns regarding the validity of approaches, usefulness and trust in such frameworks. Selecting indicators requires transparent and well-defined procedures to ensure the relevance and validity of sustainability assessments. The objective of this study, therefore, was to determine whether experts agree on which criteria are most important in the selection of indicators and indicator sets for robust sustainability assessments. Two groups of experts (Temperate Agriculture Research Network and New Zealand Sustainability Dashboard) were asked to rank the relative importance of eleven criteria for selecting individual indicators and of nine criteria for balancing a collective set of indicators. Both ranking surveys reveal a startling lack of consensus amongst experts about how best to measure agricultural sustainability and call for a radical rethink about how complementary approaches to sustainability assessments are used alongside each other to ensure a plurality of views and maximum collaboration and trust amongst stakeholders. To improve the transparency, relevance and robustness of sustainable assessments, the context of the sustainability assessment, including prioritizations of selection criteria for indicator selection, must be accounted for. A collaborative design process will enhance the acceptance of diverse values and prioritizations embedded in sustainability assessments. The process by which indicators and sustainability frameworks are established may be a much more important determinant of their success than the final shape of the assessment tools. Such an emphasis on process would make assessments more transparent, transformative and enduring. 相似文献
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Sylvain Giroud Alina L. Evans Isabelle Chery Fabrice Bertile Georg Tascher Justine Bertrand-Michel Guillemette Gauquelin-Koch Jon M Arnemo Jon E. Swenson Etienne Lefai Stéphane Blanc Chantal Simon 《Die Naturwissenschaften》2018,105(9-10):58
Polyunsaturated fatty acids (PUFAs) exert several important functions across organ systems. During winter, hibernators divert PUFAs from oxidation, retaining them in their tissues and membranes, to ensure proper body functions at low body temperature. PUFAs are also precursors of eicosanoids with pro- and anti-inflammatory properties. This study investigated seasonal changes in eicosanoid metabolism of free-ranging brown bears (Ursus arctos). By using a lipidomic approach, we assessed (1) levels of specific omega-3 and omega-6 fatty acids involved in the eicosanoid cascade and (2) concentrations of eicosanoids in skeletal muscle and blood plasma of winter hibernating and summer active bears. We observed significant seasonal changes in the specific omega-3 and omega-6 precursors. We also found significant seasonal alterations of eicosanoid levels in both tissues. Concentrations of pro-inflammatory eicosanoids, such as thromboxane B2, 5-hydroxyeicosatetraenoic acid (HETE), and 15-HETE and 18-HETE, were significantly lower in muscle and/or plasma of hibernating bears compared to summer-active animals. Further, plasma and muscle levels of 5,6-epoxyeicosatrienoic acid (EET), as well as muscle concentration of 8,9-EET, tended to be lower in bears during winter hibernation vs. summer. We also found lower plasma levels of anti-inflammatory eicosanoids, such as 15dPGJ2 and PGE3, in bears during winter hibernation. Despite of the limited changes in omega-3 and omega-6 precursors, plasma and muscle concentrations of the products of all pathways decreased significantly, or remained unchanged, independent of their pro- or anti-inflammatory properties. These findings suggest that hibernation in bears is associated with a depressed state of the eicosanoid cascade. 相似文献
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Environmental and Ecological Statistics - 相似文献
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Jon R Miller 《Journal of Environmental Economics and Management》1978,5(3):292-300
Concern for the acceleration in threats to species diversity in the United States led to the passage of the Endangered Species Act of 1973. In this paper, this legislation is examined in an economic context. A simple model is developed where management and constraints on economic activity are substitutes in the production of species stocks. Whether economic growth constraints (the primary policy tool of the 1973 Act) are justified for preservation purposes depends on technical substitution possibilities, relative cost ratios, and the existence of budget constraints for species management. Since the same combination of these factors will not occur for all species, it is argued that more flexibility in endangered species policy may preserve the same levels of species stocks at lower costs to society. 相似文献