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1.
A simplified method is described for processing both direct preparations and long-term cultures from the same fragment of chorionic villi. Enzyme separation of the outer trophoblast layers (used for direct preparations) from the inner mesenchymal core (used to initiate long-term cultures) facilitates the utilization of the same fragments for the two procedures, without jeopardizing the success of either method. This has proved useful in cases where the sample was so small that only one method of chromosome preparation may have been possible using other techniques. 相似文献
2.
R.F. Griffiths 《Process Safety and Environmental Protection》1996,74(4):289
3.
R.F. Griffiths 《Process Safety and Environmental Protection》1996,74(4):290
4.
Van Butsic Catalina Munteanu Patrick Griffiths Jan Knorn Volker C. Radeloff Juraj Lieskovský Daniel Mueller Tobias Kuemmerle 《Conservation biology》2017,31(3):570-580
Protected areas are a cornerstone for forest protection, but they are not always effective during times of socioeconomic and institutional crises. The Carpathian Mountains in Eastern Europe are an ecologically outstanding region, with widespread seminatural and old‐growth forest. Since 1990, Carpathian countries (Czech Republic, Hungary, Poland, Romania, Slovakia, and Ukraine) have experienced economic hardship and institutional changes, including the breakdown of socialism, European Union accession, and a rapid expansion of protected areas. The question is how protected‐area effectiveness has varied during these times across the Carpathians given these changes. We analyzed a satellite‐based data set of forest disturbance (i.e., forest loss due to harvesting or natural disturbances) from 1985 to 2010 and used matching statistics and a fixed‐effects estimator to quantify the effect of protection on forest disturbance. Protected areas in the Czech Republic, Slovakia, and the Ukraine had significantly less deforestation inside protected areas than outside in some periods; the likelihood of disturbance was reduced by 1–5%. The effectiveness of protection increased over time in these countries, whereas the opposite was true in Romania. Older protected areas were most effective in Romania and Hungary, but newer protected areas were more effective in Czech Republic, and Poland. Strict protection (International Union for Conservation of Nature [IUCN] protection category Ia‐II) was not more effective than landscape‐level protection (IUCN III‐VI). We suggest that the strength of institutions, the differences in forest privatization, forest management, prior distribution of protected areas, and when countries joined the European Union may provide explanations for the strikingly heterogeneous effectiveness patterns among countries. Our results highlight how different the effects of protected areas can be at broad scales, indicating that the effectiveness of protected areas is transitory over time and space and suggesting that generalizations about the effectiveness of protected areas can be misleading. 相似文献
5.
Justin M. Langridge R. Joel Gustafsson Paul T. Griffiths R. Anthony Cox Richard M. Lambert Roderic L. Jones 《Atmospheric environment (Oxford, England : 1994)》2009,43(32):5128-5131
The photoenhanced uptake of nitrogen dioxide (NO2) to the surface of commercially available self-cleaning window glass has been studied under controlled laboratory conditions. This material is one of an array of modern building products which incorporate titanium dioxide (TiO2) nanoparticles and are finding increasing use in populated urban areas. Amongst the principal drivers for the use of these materials is that they are thought to facilitate the irreversible removal of pollutants such as NO2 and organic molecules from the atmosphere and thus act to remediate air quality. While it appears that TiO2 materials do indeed remove organic molecules from built environments, in this study we show that the photoenhanced uptake of NO2 to one example material, self-cleaning window glass, is in fact accompanied by the substantial formation (50–70%) of gaseous nitrous acid (HONO). This finding has direct and serious implications for the use of these materials in urban areas. Not only is HONO a harmful respiratory irritant, it is also readily photolysed by solar radiation leading to the formation of hydroxyl radicals (OH) together with the re-release of NOx as NO. The net effect of subsequent OH initiated chemistry can then be the further degradation of air quality through the formation of secondary pollutants such as ozone and VOC oxidation products. In summary, we suggest that a scientifically conceived technical strategy for air quality remediation based on this technology, while widely perceived as universally beneficial, could in fact have effects precisely opposite to those intended. 相似文献
6.
7.
8.
George A. Griffiths 《Journal of the American Water Resources Association》1982,18(4):575-584
ABSTRACT: Specific annual suspended sediment yields and their standard deviations are presented for 47 basins of North Island, New Zealand. Most of the variance in yields is explained by catchment mean rainfall. Rivers with similar flow range have similar suspended sediment concentration ratings, independent of differing watershed lithology and regolith, except for six basins having an abundance of soft fine sediments. Prediction equations for yield and its standard deviation are derived for four essentially arbitrary regions. AU feature rainfall as the independent variable. Differences between regions may owe to variations in intensity, frequency, and duration patterns of storms and, in one area, to bed material size as well. The temporal distribution of annual yields from a basin m be modeled by a two-parameter lognormal function: the prediction equations above may be used to evaluate this function at a site for which suspended sediment data are unavailable. 相似文献
9.
Heckmann LH Griffiths BS Caul S Thompson J Pusztai-Carey M Moar WJ Andersen MN Krogh PH 《Environmental pollution (Barking, Essex : 1987)》2006,142(2):212-216
Studies on the effect of genetically modified Bacillus thuringiensis (Bt) crops on true soil dwelling non-target arthropods are scarce. The objective of this study was to assess the influence of a 4-week exposure to two Bt maize varieties (Cry1Ab) Cascade and MEB307 on the collembolan Protaphorura armata. For comparison three non-Bt maize varieties, Rivaldo (isogenic to Cascade), Monumental (isogenic to MEB307) and DK242, and two control diets based on baker's yeast (uncontaminated and contaminated with Bt toxin Cry1Ab) were also tested. Due to a lower C:N ratio, individuals reared on yeast performed significantly better in all of the measured endpoints than those reared on maize. P. armata performed equally well when reared on two Bt and three non-Bt maize varieties. Although there were no negative effects of Bt maize in this experiment, we recommend future studies on Bt crops to focus on species interactions in long-term, multi-species experiments. 相似文献
10.
The alien mussel Mytilus galloprovincialis invaded sand banks in Langebaan Lagoon on the west coast of South Africa in the mid-1990s. However, by 2001 these beds had
completely died off, with only empty shells and anoxic sand remaining. In an effort to prevent the re-settlement of this aggressive
invader, all dead mussel shells were then cleared. This study considered the impacts of the invasion and subsequent die-off
on natural benthic communities. Community composition differed significantly between non-invaded and invaded areas (ANOSIM,
R = 0.685 and P < 0.01) as the physical presence of mussel beds created a new habitat that promoted invasion by indigenous rocky-shore species.
This dramatically increased faunal biomass from 1,132.9 g m−2 ± 3,454.7 SD to 53,262.4 g m−2 ± 23,052.6 SD and species richness from 38 to 49 species. Following the die-off of the mussel beds, communities remained
significantly different between non-invaded areas and those in which mussel shells remained (ANOSIM, R = 0.663 and P < 0.01). Species richness was significantly greater in non-invaded areas (18 species) than in uncleared areas with remnant
shells (four species) (Kruskal–Wallis ANOVA H
2,36 = 10.8964 and P = 0.032), as the previously dominant rocky-shore species became smothered by sediment and the compacted shells formed an
impermeable layer excluding sandy-shore burrowing organisms. After the shells were cleared, 50% of the sandy-shore species
associated with non-invaded areas returned within 5 months, but community structure still remained significantly different
to non-invaded areas (ANOSIM, R = 0.235 and P > 0.05). Invasion thus dramatically altered natural communities and although the subsequent removal of the dead mussel shells
appears to have aided recovery, community composition remained different from the pre-invasion state after 5 months. 相似文献