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Antonella?De?DonnoEmail author Tiziana?Grassi Francesco?Bagordo Adele?Idolo Francesca?Serio Giovanni?Gabutti 《Food and environmental virology》2012,4(2):81-88
The purpose of this study was to demonstrate that shellfish can be used to detect enteric viruses in marine waters where they
are present at very low concentrations. Aqua-cultured mussels were placed in the sea just off the mouth of a drainage channel
affected by human and animal faecal contamination. Samples were taken from the channel, the sea and the mussels at intervals
over two 4-week periods. The samples were tested to verify the presence of both rotaviruses and E. coli. Rotaviruses were detected by Real Time-PCR, typed by multiplex PCR and subsequently sequenced. E. coli was enumerated in water matrices by a filtering method and in mussels by the MPN method. The presence of E. coli in the examined matrices demonstrates contamination of faecal origin throughout the studied environments. Rotaviruses were
recorded in channel waters, but not in sea water. In both experiments, rotaviruses were detected in mussels 21 and 28 days
after being placed in the sea water off the channel mouth. The use of mussels thus enabled the detection of rotaviruses in
waters where the high dilution rendered direct investigation impossible. This study indicates that mussels can be used in
marine virological surveillance programs. 相似文献
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Marco Basile Antonio Romano Andrea Costa Mario Posillico Daniele Scinti Roger Aldo Crisci Ranieri Raimondi Tiziana Altea Vittorio Garfì Giovanni Santopuoli Marco Marchetti Sebastiano Salvidio Bruno De Cinti Giorgio Matteucci 《Die Naturwissenschaften》2017,104(9-10):80
Many small terrestrial vertebrates exhibit limited spatial movement and are considerably exposed to changes in local environmental variables. Among such vertebrates, amphibians at present experience a dramatic decline due to their limited resilience to environmental change. Since the local survival and abundance of amphibians is intrinsically related to the availability of shelters, conservation plans need to take microhabitat requirements into account. In order to gain insight into the terrestrial ecology of the spectacled salamander Salamandrina perspicillata and to identify appropriate forest management strategies, we investigated the salamander’s seasonal variability in habitat use of trees as shelters in relation to tree features (size, buttresses, basal holes) and environmental variables in a beech forest in Italy. We used the occupancy approach to assess tree suitability on a non-conventional spatial scale. Our approach provides fine-grained parameters of microhabitat suitability and elucidates many aspects of the salamander’s terrestrial ecology. Occupancy changed with the annual life cycle and was higher in autumn than in spring, when females were found closer to the stream in the study area. Salamanders showed a seasonal pattern regarding the trees they occupied and a clear preference for trees with a larger diameter and more burrows. With respect to forest management, we suggest maintaining a suitable number of trees with a trunk diameter exceeding 30 cm. A practice of selective logging along the banks of streams could help maintain an adequate quantity of the appropriate microhabitat. Furthermore, in areas with a presence of salamanders, a good forest management plan requires leaving an adequate buffer zone around streams, which should be wider in autumn than in spring. 相似文献
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Colombo A Benfenati E Bugatti SG Celeste G Lodi M Rotella G Senese V Fanelli R 《Chemosphere》2011,85(11):1719-1724
Polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDDs/Fs) were analyzed in samples of the emissions from a secondary aluminum smelter (ALS) and soil samples around the plant. The purpose was to estimate the impact of the emissions on the surrounding environment.PCDD/F soil concentrations were higher in the proximity of the plant, exceeding the limit adopted in Italy in soils for green areas and residential uses and the upper limit of several reference concentrations. The most contaminated sites were less than 500 m from the plant and the dioxin concentration with the distance from the ALS.Principal component analysis (PCA) showed that emissions from the ALS were the source of PCDD/F contamination in the soils closest to the plant. Multivariate data analyses such as PCA are therefore useful to identify sources of emission causing contamination. 相似文献
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Marino Tiziana Galiano Francesco Simone Silvia Figoli Alberto 《Environmental science and pollution research international》2019,26(15):14774-14785
Environmental Science and Pollution Research - The possibility of replacing traditional toxic solvents normally employed during the preparation of polymeric membranes with greener alternatives... 相似文献
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Cinzia Perrino Silvia Canepari Maria Catrambone Stefano Dalla Torre Elena Rantica Tiziana Sargolini 《Atmospheric environment (Oxford, England : 1994)》2009,43(31):4766-4779
A ten-month field study aimed to determine the contribution of natural events (i.e. sea-salt and mineral dust events) to urban PM concentration was carried out at six sampling sites in Central Italy (Lazio region). Four indicators have been used to identify natural events during the period of the study. The first one is constituted by the ratio between number of particles in the coarse to the accumulation mode. It is simple, cheap, and the information are given in quasi-real time, but the nature of the event (sea-salt or mineral dust) is not detectable. The second indicator relies on the chemical analysis of the collected PM by X-ray fluorescence (XRF) and allows a robust identification of sea-salt and crustal components. The third one is based on diagnostic ratios of elemental fractions: Mgextractable/Tiresidue for sea-salt and Tiresidue/Sbresidue for mineral dust. It requires skilled staff but it is most accurate and sensible. The last indicator, constructed on the basis of natural radioactivity data, is not diagnostic for the nature of the event but it is able to estimate the increase in PM concentration with respect to the expected concentration in the absence of natural events.The relevance of natural events and the variations in PM concentration and composition during the study are discussed. The joined use of the four indicators allowed the identification of about 20 natural PM episodes. In general, sea-salt aerosol events did not cause exceedance of the daily EU limit value for PM10. Saharan dust events, instead, were in most cases responsible for the exceedance of the limit value at all stations. 相似文献
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Tiziana Ulian Moctar Sacandé Alex Hudson Efisio Mattana 《Journal of Environmental Planning and Management》2017,60(4):668-683
Through the MGU – Useful Plants Project (2007–2015) led by the Royal Botanic Gardens Kew, high-quality seed collections and research information have been gathered on useful indigenous plants selected by communities in Botswana, Kenya, Mali, South Africa, and Mexico. Local communities were trained in seed conservation, plant propagation, and planting activities, while revenue generation was promoted directly through the sustainable use of plants and plant products and indirectly through wider environmental and cultural services. The success of this project was determined by its participative approach, involving local communities in plant conservation activities and using plant research to support it. However, the promotion of sustainable use and income generation highlighted issues that confirmed the importance of using a ‘holistic approach’ to address the objective of poverty reduction and contribute to improved livelihoods in the communities. 相似文献
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Maria E. Noce Tiziana Belfiore Stefano Scalercio Veronica Vizzarri Nino Iannotta 《Journal of environmental science and health. Part. B》2013,48(5):442-448
Decreasing pesticide use in olive groves is central to controlling pathogens and pests such as Bactrocera oleae. This has led to the development of mass trapping devices which not only minimize pesticide use but, with improved efficacy of attractants, also decrease costs associated with pest control and ensures that the quality of olive oil is safe for human consumption. This study was undertaken to test a new device which utilizes reduced quantities of both insecticide (lambda-cyalothrin) as well as the female olive fly pheromone (1,7-dioxaspiro-(5.5)-undecane). The new device was tested against an older device manufactured by the same company. The use of plastic polymers as substrate for encapsulating the pheromone allowed for a slower pheromone release, prolonging the efficacy and duration and thus reducing costs. The density of adult populations was monitored using yellow chromotropic traps that were checked every ten days and the degree of olive infestation, as determined by preimago stages, was assessed by analyzing 100 drupes per plot. Infestation analyses were performed every ten days. The control plot had the lowest density of adults and the highest drupe infestation rate. The new devices were more effective than the older devices in both attracting adults and controlling infestation of drupes. Moreover, the new devices containing reduced amounts of pheromone and insecticide were cheaper and exhibited longer functional efficacy. In addition to the slower release of attractants, the plastic polymers used in these newer devices were also more resistant to mechanical and weather degradations. Results demonstrate that mass trapping can indeed be an effective means of controlling B. oleae via eco-sustainable olive farming. 相似文献
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