Using Multiple Antibiotic Resistance and land use characteristics to determine sources of fecal coliform bacterial pollution

Multiple Antibiotic Resistance (MAR) analysis and regression modeling techniques were used to identify surface water areas impacted by fecal pollution from human sources, and to determine the effects of land use on fecal pollution in Murrells Inlet, a small, urbanized, high-salinity estuary located between Myrtle Beach and Georgetown, South Carolina. MAR analysis was performed to identify areas in the estuary that are impacted by human-source fecal pollution. Additionally, regression analysis was performed to determine if an association exists between land use and fecal coliform densities over the ten-year period from 1989 to 1998. Land-use variables were derived using Geographic Information System (GIS) techniques and were used in the regression analysis.MAR analyses were conducted by comparing the frequency and patterns of antibiotic resistance found in Escherichia coli isolates derived from surface water samples and from sewage sources in the Murrells Inlet sewage collection system. The MAR results suggest that the majority of the fecal pollution detected in the Murrells Inlet estuary may be from non-human sources, including fecal coliforms isolated from areas in close proximity to high densities of active septic tanks.A MAR Index, which measures the frequency of antibiotic resistance, was calculated for each of twenty-three water samples and nine sewage samples. The antibiotic resistance pattern comparisons were performed using cluster analysis. Although the MAR indices indicated that several surface water sites had potential human-source contamination, the cluster analysis suggests that only one sampling site had MAR patterns that were similar to those found in the sewage samples. This site was in close proximity to several large pleasure boats as well as a sewage collection system lift station, but was not near areas with active septic tanks. The results of the regression analysis also suggest that sewage sources and rainfall runoff from urbanized areas may contribute to fecal pollution in the estuary.     

Inter and intra-population variation in shoaling and boldness in the zebrafish (<Emphasis Type="Italic">Danio rerio</Emphasis>)

Population differences in anti-predator behaviour have been demonstrated in several species, although less is known about the genetic basis of these traits. To determine the extent of genetic differences in boldness (defined as exploration of a novel object) and shoaling within and between zebrafish (Danio rerio) populations, and to examine the genetic basis of shoaling behaviour in general, we carried out a study that involved laboratory-raised fish derived from four wild-caught populations. Controlling for differences in rearing environment, significant inter-population differences were found in boldness but not shoaling. A larger shoaling experiment was also performed using one of the populations as the basis of a North Carolina type II breeding design (174 fish in total) to estimate heritability of shoaling tendency. A narrow-sense heritability estimate of 0.40 was obtained, with no apparent dominance effects.     

英国塘鹅蛋中多氯联苯同类物的时空变化趋势

1977～1998年间,在苏格兰西部的艾尔萨克雷格对收集到的塘鹅蛋中的多氯联苯(PCB)同类物进行了回顾性分析.对一些年份所收集的塘鹅蛋中每年选择8～10个对其PCB同类物浓度范围分别进行了分析.所有的同类物在几年之内浓度都降在低,但不同的同类物降解速率不同.     

Ecosystem management to achieve ecological sustainability: The case of South Florida

The ecosystems of South Florida are unique in the world. The defining features of the natural Everglades (large spatial scale, temporal patterns of water storage and sheetflow, and low nutrient levels) historically allowed a mosaic of habitats with characteristic animals. Massive hydrological alterations have halved the Everglades, and ecological sustainability requires fundamental changes in management.The US Man and the Biosphere Human-Dominated Systems Directorate is conducting a case study of South Florida using ecosystem management as a framework for exploring options for mutually dependent sustainability of society and the environment. A new methodology was developed to specify sustainability goals, characterize human factors affecting the ecosystem, and conduct scenario/consequence analyses to examine ecological and societal implications. South Florida has sufficient water for urban, agricultural, and ecological needs, but most water drains to the sea through the system of canals; thus, the issue is not competition for resources but storage and management of water. The goal is to reestablish the natural system for water quantity, timing, and distribution over a sufficient area to restore the essence of the Everglades.The societal sustainability in the Everglades Agricultural Area (EAA) is at risk because of soil degradation, vulnerability of sugar price supports, policies affecting Cuban sugar imports, and political/economic forces aligned against sugar production. One scenario suggested using the EAA for water storage while under private sugar production, thereby linking sustainability of the ecological system with societal sustainability. Further analyses are needed, but the US MAB project suggests achieving ecological sustainability consistent with societal sustainability may be feasible.     

Impact of Land Use on Soluble Organic Nitrogen in Soil

Although it has been hypothesized that soluble organic nitrogen (SON) plays a central role in regulating productivity in some terrestrial ecosystems, the factors controlling the size of the SON pool in soil remain poorly understood. Therefore our principal aim in this work was to assess the impact of seven different land use systems (rough and managed grassland, deciduous and coniferous woodland, heathland, wetland and tilled land) on the size of the SON and inorganic N (NO ₃ ^– , NH ₄ ⁺ ) pools in the surface soil layer (0–15 cm). After extraction with deionised water, we found that in most cases the size of the water extractable organic N (WEON) pool was similar in size to the inorganic N pool. In contrast, the KCl extractable organic N (KClEON) pool constituted the dominant form of soluble N in soils under all land uses, perhaps indicating that significant amounts were held on the soil exchange phase. In contrast to inorganic N, which varied significantly with land use, the size of the KClEON and WEON pool was similar for all land uses with the exception of KClEON in tilled land, where significantly lower amounts were observed. We conclude that SON constitutes an important soil N pool in a broad range of land uses, and that its role in microbial N assimilation, plant nutrition and ecosystem responses to atmospheric N deposition warrants further attention. SAFRD, University of Newcastle, Newcastle-upon-Tyne, NE1 7RU, U.K.     

Innovative aspects of environmental chemistry and technology regarding air,water, and soil pollution

Environmental Science and Pollution Research -     

Insight into selected emerging micropollutant interactions with wastewater colloidal organic carbon: implications for water treatment and analysis

Environmental Science and Pollution Research - This study reports how adding a membrane filter (0.45-μm cellulose nitrate filter) between a glass fibre filter and the solid phase extraction...     

Assessment of the Streptomyces-plant system to mitigate the impact of Cr(VI) and lindane in experimental soils

Environmental Science and Pollution Research - Phytoremediation techniques have been proposed as ecological methods to clean up contaminated sites. This study is aimed to evaluate the effect of the...