The effect of filter material on bioaerosol collection of Bacillus subtilis spores used as a Bacillus anthracis simulant

The objective of this study was to determine filter materials and extraction methods that are appropriate to use for environmental sampling of B. anthracis. Four types of filters were tested: mixed cellulose ester (MCE) with a pore size of 3 microm, polytetrafluoroethylene (PTFE) with pore sizes of 1 and 3 microm, and gelatin with a pore size of 3 microm. Bacillus subtilis var. niger endospores (also known as Bacillus globigii[BG]) were used as a surrogate for B. anthracis. Endospores were collected into Button Inhalable Aerosol Samplers with sampling times of 15 minutes, 1 hour, and 4 hours. Physical collection efficiency was determined by measuring upstream and downstream B. subtilis concentrations with an optical particle counter. Vortexing with ultrasonic agitation and vortexing with shaker agitation extraction methods were evaluated. The MCE, 1 microm PTFE, and gelatin filters provided physical collection efficiencies of 94% or greater. The 3 microm PTFE filter showed inconsistent physical efficiency characteristics between filters. Epifluorescence microscopic analysis of the gelatin filter extraction fluid revealed the presence of contamination by non-culturable bacteria. Mean differences for microbial culturability were not statistically significant for filter materials and extraction methods. However, the vortexing with shaker agitation extraction method resulted in higher total microbial counts in the extraction fluids for MCE and 1 microm PTFE filters when compared to vortexing with ultrasonic agitation. In summary, the MCE and 1 microm PTFE filters in combination with vortexing and shaker extraction demonstrated the best performance for the filter collection and extraction of BG spores.     

Bi-national assessment of the Great Lakes: SOLEC partnerships

Many administrative jurisdictions have authority over parts of the Great Lakes, sometimes with competing purposes as well as governance at differing scales of time and space. As demand increases for high quality information that is relevant to environmental managers, environmental and natural resource agencies with limited budgets must look to interdisciplinary, collaborative approaches for the collection, analysis and reporting of data. The State of the Lakes Ecosystem Conferences (SOLEC) were begun in 1994 in response to reporting requirements of the Great Lakes Water Quality Agreement between Canada and the U.S. The biennial conferences provide independent, science-based reporting on the state of health of the Great Lakes ecosystem components. A suite of indicators necessary and sufficient to assess Great Lakes ecosystem status was introduced in 1998, and assessments based on a subset of the indicators were presented in 2000. Because SOLEC is a multi-agency, multi-jurisdictional reporting venue, the SOLEC indicators require acceptance by a broad spectrum of stakeholders in the Great Lakes basin. The SOLEC indicators list is expected to provide the basis for government agencies and other organizations to collaborate more effectively and to allocate resources to data collection, evaluation and reporting on the state of the Great Lakes basin ecosystem.     

Organochlorine and mercury residues in Swainson's and ferruginous hawk eggs collected in North and South Dakota, 1974–79

Residues of organochlorine pesticides, polychlorinated biphenyls (PCBs), and mercury were measured in eggs of Swainson's hawks (Buteo swainsoni) and ferruginous hawks (B. regalis) collected in North and South Dakota during 1974–79. DDE was the most common compound detected in the eggs, but residues were below levels known to have adverse effects on reproduction. Other organochlorine compounds and mercury were found at low levels. Eggs of ferruginous hawks tended to contain more compounds with higher residues than eggs of Swainson's hawks.     

Genetic amniocentesis following multifetal pregnancy reduction to twins: Assessing the risk

Fifty-three patients who elected to reduce their pregnancies to a twin gestation in our centre are known to have subsequently undergone genetic amniocentesis. Five of these patients lost their entire pregnancy following the genetic amniocentesis procedure. This is equivalent to a 9·4 per cent pregnancy loss rate for reduced twin gestations in comparison with an expected loss rate of 2 per cent for non-reduced twin gestations.     

Re-evaluating the Contribution of Social Capital in Achieving Sustainable Development

The last decade has seen considerable interest in the concept of social capital and there have been a number of publications focused around the concept. A wide range of claims have been made for the analytic potential of social capital leading some to question the concept's continued value. We think that the concept still has considerable value if used in a careful and rigorous way. We further think that the concept has particular value when considering policy for sustainable development. However, just as sustainable development is a multi-faceted concept, this application requires a multi-faceted reading of social capital. Having concluded that a clearer typology of social capital is needed, we propose a threefold typology, adding the new category of 'bracing' social capital to the more commonly used distinction between 'bonding' and 'bridging' social capital. We also address the issues of what social capital can do and how it actually works, specifically in the context of policy for sustainable development.     

Social organization in deer: Implications for localized management

Populations of white-tailed deer (Odocoileus virginianus) inhabiting many state and national parks and suburban areas have grown to the point that they conflict with human activities. Conflicts range from destruction of vegetation through browsing to public perception that diseases carried by deer pose threats to human health. Traditional modes of hunting to control populations are inappropriate in many of these areas because of intense human development and activity. This article explores an alternative approach for population reduction based on deer social organization. Female white-tailed deer are highly philopatric and female offspring remain near their dams for life. This suggests that a population expands slowly as a series of overlapping home ranges in a form analogous to the petals on a rose. Incorporating the rose petal concept into a model of population growth shows that removal of deer by family unit can potentially alleviate conflicts in localized areas for as many as 10–15 yr.     

Cultural landscapes and environmental ethics: The case of puslinch township's historic roadside trees

Conclusion I have argued, then, that is simplistic and potentially dangerous to suppose that a contractarian model can adequately account for all of the moral relations in agriculture or serve singularly as a basis for policy. Conversely, I do not naively argue that we ought simply to replace a contractarian based agricultural ethic with an ethic of care such as the one I have outlined. A single farmer may be a spouse, parent, son or daughter, caretaker of land, keep of animals, business operator and partner, employer, processor, and consumer. Agriculture itself encompasses all of these relations. Both our ethical systems and social and economic policies must reflect and build on this diversity of contexts and associated practices.     

Insecticide pollution of Indian rivers

Summary Rivers are the main source of water in India, and are particularly used for agricultural irrigation and drinking water supply. As most of the rivers pass through agricultural fields, they are subject to contamination with the different insecticides used for crop protection. Residues of persistent organochlorines, which are still used in large quantities in India, are found in water from many Indian rivers. In certain rivers, the concentrations of DDT, aldrin and heptachlor are often present in excess of their guideline limits. Although the concentration level of gamma-HCH is well below the guideline limit, the accumulation of the carcinogenic beta isomer is a matter of great concern. A few organophosphorus insecticides have also been detected in river water. Recently, some organochlorine insecticides have been banned from use in India. The use of new, readily biodegradable insecticides and biocides in agriculture and public health programmes offers some optimism.Drs Satya P. Mohapatia, Vijay T. Gajbhiye and Narendra R. Agnihotri all work within the Division of Agricultural Chemicals at the above address. Dr Manju Raina is employed by the Ganga Project Directorate of the Ministry of Environment and Forests, New Delhi 11003, India.