Genetic sex determination in <Emphasis Type="Italic">Astatotilapia calliptera</Emphasis>, a prototype species for the Lake Malawi cichlid radiation

East African cichlids display extensive variation in sex determination systems. The species Astatotilapia calliptera is one of the few cichlids that reside both in Lake Malawi and in surrounding waterways. A. calliptera is of interest in evolutionary studies as a putative immediate outgroup species for the Lake Malawi species flock and possibly as a prototype ancestor-like species for the radiation. Here, we use linkage mapping to test association of sex in A. calliptera with loci that have been previously associated with genetic sex determination in East African cichlid species. We identify a male heterogametic XY system segregating at linkage group (LG) 7 in an A. calliptera line that originated from Lake Malawi, at a locus previously shown to act as an XY sex determination system in multiple species of Lake Malawi cichlids. Significant association of genetic markers and sex produce a broad genetic interval of approximately 26 megabases (Mb) using the Nile tilapia genome to orient markers; however, we note that the marker with the strongest association with sex is near a gene that acts as a master sex determiner in other fish species. We demonstrate that alleles of the marker are perfectly associated with sex in Metriaclima mbenjii, a species from the rock-dwelling clade of Lake Malawi. While we do not rule out the possibility of other sex determination loci in A. calliptera, this study provides a foundation for fine mapping of the cichlid sex determination gene on LG7 and evolutionary context regarding the origin and persistence of the LG7 XY across diverse, rapidly evolving lineages.     

Use of tree aggregations in forest ecology and management

Two recent studies using tree aggregations to analyze forest age-structure stability and past forest structure are flawed. A better understanding of aggregation dynamics is needed before aggregation analysis is used in forest management.     

LACTOSE NEGATIVE ESCHERICHIA COLI FROM RANGELAND STREAMS: SOURCE,ANTIBIOTIC RESISTANCE,AND COLICINOGENICITY1

ABSTRACT: Lactose-negative Escherichia coil from cattle feces appeared as yellow, atypical colonies on m-FC medium plates with water samples from rangeland streams. The lactose-negative E. coil may impact stream water quality analyses if infrequent samples are collected; are less antibiotic resistant than the lactose-positive E. coili isolated from rangeland streams; and are colicinogenic toward all the laboratory strains of E. coil examined and toward 61 percent of the lactose-positive E. coil rangeland-stream isolates that were tested. This latter result could explain the potentially low degree of antibiotic resistance transfer from lactose-positive to lactose-negative E. coil. In addition, the colicinogenicity of the lactose-negative E. coil may interfere with microbiological water quality analyses that depend upon lactose fernientations with mixed populations of coliforms.     

Graduated driver licensing: the New Zealand experience

In New Zealand, on 1 August 1987, a three-stage graduated driver licensing (GDL) system that applied to all new drivers aged 15-24 years was introduced. The essential elements of GDL were a 6-month learner license (supervised driving) and an 18-month restricted license stage (with restrictions on night driving and carrying passengers). A blood alcohol limit of 0.03 mg% applied at both stages. EVALUATION STUDIES: Early studies indicated that young people were reasonably accepting of the restrictions, with the passenger restriction being the least acceptable. Problems of compliance with the restricted license driving restrictions were reported. Evaluations of the impact of the graduated driver licensing (GDL) on serious traffic-related injury showed that up until 1991-1992, an 8% reduction could be attributed to GDL. At this time, it was considered that reduced exposure was the main reason for this reduction. However, the number of fatalities and hospital admissions among young people continued to decline, as did the population rate and the rate per number of licensed drivers among the young driver age group. A further evaluation study showed that drivers with a restricted license had a smaller proportion of crashes at night, and with passengers, compared with drivers licensed before GDL. IMPACT OF GDL: These results suggested that GDL restrictions had contributed to the reduction in crashes among young people and that it was not simply a case of reduced exposure to risk. An update of the most recent crash statistics indicated that, compared with older age groups, the fatal and serious injury crash rate among young people has remained substantially below the pre-GDL level. This suggests that the impact of GDL has not diminished over time.     

'Making a wish': Children and the local landscape

The links between children's rights and their participation in environmental decision-making is a growing field of interest. This paper describes findings from a six-month in-depth pilot study with a small group of children aged 6-10 years located in a village in the northeast of England. The focus of this paper is on the relationship of these children with their local landscape and how they felt about involvement in decision-making that might affect that environment. The methodology was child-centred responding to the 'languages' of children. Two main findings are discussed in this paper: (a) issues of boundary were particularly important in physical and emotional terms for both children and parents and this had little to do with the rurality of the location; (b) children believe they have special knowledge about the environment, which they may express using a range of communication methods. The author reflects on what implications this might have for the design and layout of local environments generally.     

Stable carbon isotope variability in marine macrophytes and its implications for food web studies

Stable carbon isotope ratios (¹³C) of eelgrass Zostera marina and of kelp Laminaria longicruris showed considerable variation in time and space. The isotopic composition of Z. marina varied seasonally from a mean of-6 for leaves formed in June to a mean of-10 for leaves formed in February. The maximum range for individual leaves was from-5 to-11.4. Once a leaf was fully formed, its isotopic composition appeared not to change. In L. longicruris there was no clear seasonal pattern of variation, but in any given blade there was a spatial pattern of variation, with the thickened central band tending to be least negative and the margins most negative. In one blade the range was from-12 to-20. Since this range overlaps values that are found in various other macrophytes and in seston, the value of the stable carbon isotope ratio as a tracer in food webs involving macroalgae is questioned. For Z. marina, in which the values are seasonally predictable, the technique may be useful if interpreted with care. The most probable explanation of variation in the ratios is differential storage of biochemical components of different isotopic compostion.