Relationships among host microbiota,parasite resistance or tolerance,and host fitness

Article impact statement: Parasite-induced shifts in host microbiota that lead to parasite resistance or tolerance may have unintended consequences for host fitness.     

Effects of new technologies with special regard for the implementation process per se

The aim of this study was to investigate the effects of the implementation process to new technologies [e.g. drawing board/computer-aided design (CAD), microfiche/electronic data processing] on the employees concerned. Since the emphasis of the study lay in the intrinsic relationship of the implementation process with stress reactions, the parameters were measured at the place of work in a longitudinal design. Employees (n = 279) in seven companies (work fields: CAD, clerical work, telephone information desk) were tested three times — two months prior to, during, and 12 months after implementation. Aspects of strain and satisfaction were taken into account at each measurement phase. A special test-instrument was designed to describe the ’style of implementation‘ (manner in which implementation took place) particular to each company. The level of strain was found to increase during the implementation process. The amount of this increase was found to correlate with the company's style of implementation as well as with the type of the individual's work activity. The highest levels were found among those employees little included in the implementation process and those whose work is described as monotonous.     

Noncovalent bonds are key mechanisms for the cohesion of barnacle (Balanus crenatus) adhesive proteins

Cement of the barnacle species, Balanus crenatus, was isolated and separated by SDS-PAGE. Reductive and nonreductive conditions yielded the same result of six major proteins (in the range up to 100 kDa) occurring as two triplets. A third triplet of lower molecular mass was faintly visible. The presence of a reductive agent had only little impact on the solubility of B. crenatus cement. The strong effect of the denaturing conditions (the presence of SDS and the incubation at elevated temperature) on the solubility of the barnacle cement and the absence of disulfide signals in Raman spectra suggest a complex structure of the barnacle cement noncovalently bound. It can be speculated that this protein complex in its quaternary structure is formed by small subunits and is equivalent to the globular cement structures previously detected.     

New jet-aeration system using ‘Supercavitation’

Background, aim and scope  

A newly developed fine bubble aeration system, by which air is transferred under supercavitation conditions, shows a clearly better performance than traditional, well-known aerators that rely on the jet-pump principle and its performance can be compared to oxygen transfer rates achieved in membrane and foil plate aerators.     

Survival of selected pathogens in diluted sludge of a thermophilic waste treatment plant and in NaCl-solution under aerobic and anaerobic conditions

Decimal reduction times (DRT or D-value) of Campylobacter jejuni, Salmonella enterica (formerly Salmonella choleraesuis) serovar Senftenberg, Escherichia coli, and Listeria monocytogenes were determined in two different matrices, diluted fermenter sludge (DFS) and 0.95% NaCl-solution (NaCl) at 50 degrees C, both under aerobic and anaerobic conditions. Depending on aeration, matrix composition, and the respective organism, the D-values varied between 10min and more than 15h. Generally the viability of bacteria decreased faster in DFS compared to NaCl-solution and under aerobic compared to anaerobic conditions. After 24h no viable cells could be detected in DFS, both under aerobic as well as under anaerobic conditions, whereas viable cells were still found in NaCl solutions. In both matrices the detection limits determined by means of PCR-based and classical microbiological methods were compared and pointed to lower detection limits of the latter methods. Results of the present investigation show that test organisms were far from surviving several days in DFS whereas hydraulic retention times normally used for thermophilic anaerobic digestion are in the range of 2 weeks. However, an underestimation of survival rates of the test organisms seems probable when applying aerobic standard methods.     

Influence of near-to-nature stormwater management on the local water balance using the example of an urban development area

Near-to-nature stormwater management aims at replicating the quasi-natural local water balance and preserving the ecosystem's integrity of affected waters. Surface waters in the urban areas of Trier-Petrisberg are managed by a separate sewer system in conjunction with a complex retention system. To investigate the effect of this alternative rainwater management practice on the local water balance, a differentiated discharge and groundwater monitoring network with a high spatio-temporal measurement resolution has been implemented within the watershed. Additional information regarding the proportion and spatial distribution of discharge-generating surfaces was provided through visual interpretation of aerial photographs. As a result of this analysis, groundwater levels were found to not be significantly affected by an increase of local sealed surfaces, and the ecologically-justifiable maximum discharge in the tributary was infrequently exceeded. Regarding further building development and climate change, the hydrological load capacity of the retention basins will provide effective rainwater management, even with respect to increasing precipitation intensities and frequencies.     

Mortality of the scleractinian coral <Emphasis Type="Italic">Cladocora caespitosa</Emphasis> during a warming event in the Levantine Sea (Cyprus)

A mortality event of Cladocora caespitosa corals and the extent of bleaching, necrosis and pigmented areas in the colonies were studied at the southeastern coast of Cyprus during a prolonged period of higher than average sea temperature anomalies (summer/autumn 2012). With the use of scuba diving and image analysis software, we monitored the extent of mortality of 29 colonies of C. caespitosa by measuring and comparing the area percentage of healthy tissue, affected tissue (bleached, necrotic) and older mortality events (encrusted skeleton). In September 2012, on average, 24 % of the colonies surface area was affected (bleaching and/or necrosis). In October 2012, C. caespitosa showed on average 26.3 % of the colony surface area affected, evidence of continuing deterioration. At the same time, 10 % (3 of 29) of the colonies showed an increase in the pigmentation of previously bleached polyps in small and marginal areas (6–8 %). Irrespective of the amount, the regaining of pigments recorded is considered an important find. Corals and marine organisms in general in the Levantine Sea are affected greatly by warming events, to the extent where a very small percentage of polyps/colonies show resilience under thermal stress. Natural bleaching of C. caespitosa, even though limited to a few colonies and very small portions of tissue/polyps, was documented for the first time in the Levantine Sea. We conclude that temperature anomalies are associated with the mortality event. Whether prolonged higher temperature is the direct cause, or whether it acts synergistically with other factors should be the subject of further investigations.     

Assessment of bioclimatic conditions on Crete Island,Greece

The objective of this study was to assess and analyze the human bioclimatic conditions of Crete Island, by applying two human thermal indices: physiological equivalent temperature (PET), derived from the Munich Energy-balance Model for Individuals human energy balance model, and Universal Thermal Climate Index (UTCI), based on the Fiala multi-node model of human thermoregulation. Human bioclimatic studies provide a framework for considering the effects of climatic conditions on human beings and highlighting the social/economic factors that mitigate or amplify the consequences of environmental changes. In order to estimate the thermal effect of the environment on the human body, it has been considered that the total effects of all thermal components, not only of individual parameters, should be taken into account. The climatic data (air temperature, relative humidity, cloudiness, wind speed) used in this study were acquired from the archives of the Hellenic National Meteorological Service, regarding ten meteorological stations in Crete Island. These data, covering the 30-year period 1975–2004, were used for the calculation of PET and UTCI in order to assess thermo-physiological stress levels. The findings of this analysis, such as bioclimatic diagrams, temporal and spatial distributions of PET and UTCI as well as trends and variability, will help stake holders to understand and interpret the island’s current bioclimate, in order to make any necessary adaptations and become more resilient to the foreseen climate change.