Interaction between herbivore defense and microbial signaling: bacterial quorum-sensing compounds weaken JA-mediated herbivore resistance in Nicotiana attenuata

Bacteria play important roles in plant–herbivore interactions and communicate with each other with chemical signals, often N-acylhomoserine lactones (AHL). Plant responses to these signals may influence resistance to microbial attack, but the effects of these signals on herbivore defense are unstudied. To determine whether AHL influence jasmonate (JA)-mediated herbivore resistance in Nicotiana attenuata, we treated wild-type (WT) and JA-deficient genotypes (antisense expression of NaLOX3) with N-hexanoyl-dl-homoserine lactone (C6-HSL) and measured the performance of Manduca sexta larvae. Larval mass gain on C6-HSL-treated WT plants was equivalent to that on non-treated NaLOX3-silenced plants, but significantly 4.1-fold larger than on untreated WT plants. Mass gain was unaffected by C6-HSL treatment of NaLOX3-silenced plants. Microarray analysis of the plants elicited with C6-HSL and JA inducing fatty acid–amino acid conjugates revealed a down-regulation of a proteinase inhibitor in the C6-HSL-treated WT plants. The results therefore suggest that the increased performance of M. sexta was due to direct or indirect effect of C6-HSL on JA-mediated defenses.     

Semiparametric space–time survival modeling of chronic wasting disease in deer

In this paper, we propose a semiparametric survival model to investigate the pattern of spatial and temporal variation in disease prevalence of chronic wasting disease (CWD) in wild deer in Wisconsin over the years 2002 and 2006. The semiparametric survival model we suggested allows to build a more flexible model than the parametric model with fewer parametric assumptions by modeling the baseline hazard using a Gamma process prior. Based on the proposed model, we investigate the geographical distribution of CWD, and assess the effect of sex on disease prevalence. We use a Bayesian hierarchical framework where latent parameters capture temporal and spatial trends in disease incidence, incorporating sex and spatially correlated random effects. We also propose bivariate baseline hazard which change over age and time simultaneously to adopt different effects of age and time on the baseline hazard. Inference is carried out by using MCMC simulation techniques in a fully Bayesian framework. Our results suggest that disease has been spreaded mainly in the disease eradication zone and male deer show a significantly higher infection probability than female deer.     

Evidence for broadcast spawning as well as brooding in the scleractinian coral Pocillopora damicornis

The reproductive activity of 88 colonies of Pocillopora damicornis at Rottnest Island, Western Australia, was examined over 12 mo (20 August 1988 to 20 August 1989). Larvae were found close to the three new moons of February, March and April, 1989, but in large numbers only during March; 67% of all corals produced larvae. There was no obvious connection between gametogenesis and the production of brooded larvae; however, those corals which did not produce, larvae also did not produce ova but did produce, sperm, and must be considered male colonies. All colonies which produced ova went on to produce planulae. Spermatogenesis occurred throughout the year, but oogenesis occurred between September 1988 and April 1989. Dramatic declines in the percentage of polyps containing eggs and sperm occurred in early February and early April 1989. On these dates the amount of lipid in the tissue of the corals decreased, which suggests that the decline in the percentage of polyps with sperm and eggs was not due to the gametes being resorbed by the polyps. Therefore, P. damicornis at Rottnest Island appears to be both a brooder and a broadcaster with asexual reproduction through brooded larvae and sexual reproduction through spawning of gametes. This accounts for earlier evidence in the literature that some input from sexual reproduction had been occurring in the populations of P. damicornis at Rottnest Island. There is no direct evidence for spawning, as broadcasting of gametes has not been observed, but the indirect evidence provides a strong argument for the existence of this dual reproduction.     

Deafness and Disability—Forgotten Components of Environmental Justice: Illustrated by the Case of Local Agenda 21 in South Wales

Abstract

This paper contributes to discussions of procedural aspects of environmental justice, understood as having procedural and substantive dimensions. It argues that the struggle for environmental justice must recognize the oppression of disabled people as part of the essential broadening of the notion of citizenship, which continues to be the focus for struggle for the international disability movement. Its case study of an area of South Wales suggests that at present disabled people, and the struggles of the disability movement, do not really feature in the way environmental activists (inside and outside government) see the world. This huge omission must be addressed, but in a way that avoids interpreting disability as an administrative category, and must engage with disablement as a political and contested notion. The paper develops the significance of this contention by considering the case of Deafness, which is entirely different from hearing impairment. The paper's case study, presented as an illustration of its arguments, shows that to regard Deaf people in South Wales as part of some generic category of ‘disabled people’ would be to ignore their self-identification as a distinctive linguistic community. Moreover, there is some evidence that Deaf people have a distinctive view of, and set of concerns about, quality of life, reflecting their distinctive experience of social injustice and marginalization. This underlines the necessity for a serious engagement with disablement as a political category, and the disability movement as a struggle for social justice, within the promotion of environmental justice.     

Reflections on network governance in Africa's forestry sector

International forestry governance is an integral part of the global policy debates on how to prevent deforestation, illicit extraction, and unsustainable timber practices. Africa is an important producer of timber, yet the region is beset by a lack of capacity and other governance challenges in the management of its forestry sector. We employ a network governance analysis to examine the extent to which the evolution and operation of the Forest Stewardship Council (FSC) and la Commission des Forêts d'Afrique Centrale (COMIFAC) have addressed governance challenges. We assess the strengths and weaknesses of these two leading examples of international forestry governance by introducing recent evidence and insights from Africa. We conclude with a policy‐relevant discussion of how the FSC and COMIFAC might enhance authority, legitimacy, and effectiveness and improve forestry governance in Africa and other parts of the world.     

Cobalt and secondary poisoning in the terrestrial food chain: data review and research gaps to support risk assessment

Cobalt is a naturally occurring element found in rocks, soil, water, plants, and animals and has diverse industrial importance. It is cycled in surface environments through many natural processes (e.g. volcanic eruptions, weathering) and can be introduced through numerous anthropogenic activities (e.g. burning of coal or oil, or the production of cobalt alloys). The environmental behaviour of cobalt in terrestrial environment is relatively poorly studied and in particular where Co is used in industrial processes, the baseline information to support wider and long-term environmental impacts is widely dispersed. To support the adoption of new EU regulations on the risk assessment of chemicals, we review here the various aspects of the environmental chemistry, fate and transport of Co across environmental interfaces and discuss the toxicology and potential for bio magnification and food chain accumulation. The soil-to-plant transfer of Co appears to be viable route to expose lower trophic levels to biologically significant concentrations and Co is potentially accumulated in biomass and top soil. Evidence for further accumulation through soil-invertebrate transfer and to higher trophic levels is suggested by some studies but this is obscured by the relatively high variability of published transfer data. This variation is not due to one particular aspect of the transfer of Co in terrestrial environments. Influences are from the variability of geological sources within soil systems; the sensitivity of Co mobility to environmental factors (e.g. pH) and the variety of life strategies for metal elimination/use within biological species. Toxic effects of Co have been suggested for some soil-plant animal studies however, uncertainty in the extrapolation from laboratory to field is a major limitation.     

Modeling sediment and nitrogen export from a rural watershed in eastern Canada using the soil and water assessment tool

Watershed simulation models can be used to assess agricultural nonpoint-source pollution and for environmental planning and improvement projects. However, before application of any process-based watershed model, the model performance and reliability must be tested with measured data. The Soil and Water Assessment Tool version 2005 (SWAT2005) was used to model sediment and nitrogen loads from the Thomas Brook Watershed, which drains a 7.84 km rural landscape in the Annapolis Valley of Nova Scotia, Canada. The Thomas Brook SWAT model was comprised of 28 subbasins and 265 hydrologic response units, most of them containing agricultural land use, which is the main nonpoint nitrogen source in the watershed. Crop rotation schedules were incorporated into the model using field data collected within Agriculture and Agri-Food Canada's Watershed Evaluation of Beneficial Management Practices program. Model calibration (2004-2006) and validation (2007-2008) were performed on a monthly basis using continuous stream flow, sediment, and nitrogen export measurements. Model performance was evaluated using the coefficient of determination, Nash-Sutcliff efficiency (NSE), and percent bias (PBIAS) statistics. Study results show that the model performance was satisfactory (NSE > 0.4; > 0.5) for stream flow, sediment, nitrate-nitrogen, and total nitrogen simulations. Annual corn, barley, and wheat yields were also simulated well, with PBIAS values ranging from 0.3 to 7.2%. This evaluation of SWAT demonstrated that the model has the potential to be used as a decision support tool for agricultural watershed management in Nova Scotia.     

Re-use of remediated soils for the bioremediation of waste oil sludge

We investigated the possibility of re-using remediated soils for new bioremediation projects by spiking these soils with waste oil sludge in laboratory based microcosms. The level of Total Petroleum Hydrocarbon (TPH) reduction was high (>80%) in naturally attenuated microcosms and was not significantly improved by biostimulation, bioaugmentation and the combined treatment of bioaugmentation and biostimulation by week 12. This indicated that the observed TPH reduction might have been related to the soil's inherent hydrocarbon-degrading potential. Microbial community analysis (16S rDNA and ITS-based Denaturing Gradient Gel Electrophoresis fingerprints) confirmed the dominance of hydrocarbon degrading genera such as Alcanivorax and Scedosporium. Cluster and Shannon diversity analysis revealed similar but stable bacterial and fungal communities in naturally attenuated and amended microcosms indicating that rapid reduction in TPH may not always be accompanied by changes in soil microbial communities. This study has therefore shown that soils previously used for bioremediation can have an improved hydrocarbon degrading potential which was successfully re-harnessed for new projects. This ability to re-harness this potential is attractive because it substantially reduces operational costs as no additional bioremediation treatments are needed. It can also extend a landfill's lifespan as soils can be re-used again before landfill disposal.     

Confronting socially generated uncertainty in adaptive management

As more and more organizations with responsibility for natural resource management adopt adaptive management as the rubric in which they wish to operate, it becomes increasingly important to consider the sources of uncertainty inherent in their endeavors. Without recognizing that uncertainty originates both in the natural world and in human undertakings, efforts to manage adaptively at the least will prove frustrating and at the worst will prove damaging to the very natural resources that are the management targets. There will be more surprises and those surprises potentially may prove at the very least unwanted and at the worst devastating. We illustrate how acknowledging uncertainty associated with the natural world is necessary but not sufficient to avoid surprise using case studies of efforts to manage three wildlife species; Hector's Dolphins, American Alligators and Pallid Sturgeon. Three characteristics of indeterminism are salient to all of them; non-stationarity, irreducibility and an inability to define objective probabilities. As an antidote, we recommend employing a holistic treatment of indeterminism, that includes recognizing that uncertainty originates in ecological systems and in how people perceive, interact and decide about the natural world of which they are integral players.     

Hydrologic and Phosphorus Export Behavior of Small Streams in Commercial Poultry‐Pasture Watersheds1

Romeis, J. Joshua, C. Rhett Jackson, L. Mark Risse, Andrew N. Sharpley, and David E. Radcliffe, 2011. Hydrologic and Phosphorus Export Behavior of Small Streams in Commercial Poultry‐Pasture Watersheds. Journal of the American Water Resources Association (JAWRA) 1‐19. DOI: 10.1111/j.1752‐1688.2011.00521.x Abstract: Few watershed‐scale studies have evaluated phosphorus export in streamflow from commercial poultry‐pasture operations. Continuous streamflow and mixed‐frequency water quality datasets were collected from nine commercial poultry‐pasture (AG) and three forested (FORS) headwater streams (2.4‐44 ha) in the upper Etowah River basin of Georgia to estimate total P (TP) loads and examine variability of hydrologic response and water quality of storm and nonstorm‐flow regimes. Data collection duration ranged from 18 to 22 months, and approximately 1,600 water quality samples were collected. Significant (p < 0.1) inverse relationships were detected between peak flow response variables and both drainage area and fraction of forest cover. Order‐of‐magnitude differences in TP and dissolved reactive P (DRP) concentration were observed between AG and FORS sites and among AG sites. TP yields of FORS sites ranged from 0.01 to 0.1 kg P/ha. Yields of AG sites ranged from 0.031 to 3.17 kg P/ha (median = 0.354 kg P/ha). With 95% confidence intervals, AG yields ranged from 0.025 to 13.1 kg P/ha. These small‐watershed‐scale yields were similar to field‐scale yields measured in other studies in other regions. TP yields were significantly related to area‐weighted Mehlich‐1 soil test P concentrations (p = 0.0073) and base‐flow water sample P concentrations (p ≤ 0.0005). Water quality sampling during base‐flow conditions may be a useful screening tool for P risk‐based management programs.