Low-temperature caproate production,microbial diversity,and metabolic pathway in xylose anaerobic fermentation

● Converting xylose to caproate under a low temperature of 20 °C by MCF was verified. ● Final concentration of caproate from xylose in a batch reactor reached 1.6 g/L. ● Changing the substrate to ethanol did not notably increase the caproate production. ● Four genera, including Bifidobacterium , were revealed as caproate producers. ● The FAB pathway and incomplete RBO pathway were revealed via metagenomic analysis. Mixed culture fermentation (MCF) is challenged by the unqualified activity of enriched bacteria and unwanted methane dissolution under low temperatures. In this work, caproate production from xylose was investigated by MCF at a low temperature (20 °C). The results showed that a 9 d long hydraulic retention time (HRT) in a continuously stirred tank reactor was necessary for caproate production (~0.3 g/L, equal to 0.6 g COD/L) from xylose (10 g/L). The caproate concentration in the batch mode was further increased to 1.6 g/L. However, changing the substrate to ethanol did not promote caproate production, resulting in ~1.0 g/L after 45 d of operation. Four genera, Bifidobacterium, Caproiciproducens, Actinomyces, and Clostridium_sensu_stricto_12, were identified as the enriched caproate-producing bacteria. The enzymes in the fatty acid biosynthesis (FAB) pathway for caproate production were identified via metagenomic analysis. The enzymes for the conversion of (C_n+2)-2,3-Dehydroxyacyl-CoA to (C_n+2)-Acyl-CoA (i.e., EC 1.3.1.8 and EC 1.3.1.38) in the reverse β-oxidation (RBO) pathway were not identified. These results could extend the understanding of low-temperature caproate production.     

The role of lipids in fermentative propionate production from the co-fermentation of lipid and food waste

● Lipid can promote PA production on a target from food waste. ● PA productivity reached 6.23 g/(L∙d) from co-fermentation of lipid and food waste. ● Lipid promoted the hydrolysis and utilization of protein in food waste. ● Prevotella , Veillonella and norank _f _Propioni bacteriaceae were enriched. ● Main pathway of PA production was the succinate pathway. Food waste (FW) is a promising renewable low-cost biomass substrate for enhancing the economic feasibility of fermentative propionate production. Although lipids, a common component of food waste, can be used as a carbon source to enhance the production of volatile fatty acids (VFAs) during co-fermentation, few studies have evaluated the potential for directional propionate production from the co-fermentation of lipids and FW. In this study, co-fermentation experiments were conducted using different combinations of lipids and FW for VFA production. The contributions of lipids and FW to propionate production, hydrolysis of substrates, and microbial composition during co-fermentation were evaluated. The results revealed that lipids shifted the fermentation type of FW from butyric to propionic acid fermentation. Based on the estimated propionate production kinetic parameters, the maximum propionate productivity increased significantly with an increase in lipid content, reaching 6.23 g propionate/(L∙d) at a lipid content of 50%. Propionate-producing bacteria Prevotella, Veillonella, and norank_f_Propionibacteriaceae were enriched in the presence of lipids, and the succinate pathway was identified as a prominent fermentation route for propionate production. Moreover, the Kyoto Encyclopedia of Genes and Genomes functional annotation revealed that the expression of functional genes associated with amino acid metabolism was enhanced by the presence of lipids. Collectively, these findings will contribute to gaining a better understanding of targeted propionate production from FW.     

夏季闽江CDOM的空间分布与降解特征

利用三维荧光光谱-平行因子分析技术(EEMs-PARAFAC)以及微生物和光降解实验等方法,分析夏季闽江下游-河口区有色溶解有机质(CDOM)的组成、分布及其降解特征.结果表明,闽江下游-河口区CDOM存在三类荧光组分:类腐殖质、类酪氨酸和类色氨酸;类腐殖质是河段CDOM的主要荧光组分,在河口区随着盐度增加主要的荧光组分逐渐变为类蛋白质.CDOM的丰度变化呈现出明显的空间分布格局:河段CDOM的吸收系数a(280)较低,进入市区后有所增加,到了郊区呈现下降的趋势,而在河口区迅速下降;保守估计福州市区对闽江CDOM的贡献为8%.河段a(280)易被微生物降解和光降解,降解率分别为(28±8)%和(44±7)%,其生物可利用性和光化学活性远高于受海源CDOM影响的河口区;类腐殖质、类酪氨酸和类色氨酸荧光组分在河段具有较高的光化学活性,降解率分别为(75±0.5)%、(58±21)%和(73±3)%,但不易被微生物降解,而且在28 d微生物培养后出现类腐殖质的累积.     

包埋固定化海洋硅藻吸附材料的制备及其对水中铅离子的吸附特性研究

采用海藻酸钠(SA)凝胶包埋法对海洋硅藻藻粉进行固定化,考察了藻粉用量、海藻酸钠浓度、Ca Cl2质量分数、交联时间及小球粒径对固定化小球吸附铅离子性能的影响,并研究了这种吸附材料对Pb~(2+)的吸附特性.结果表明,固定化海洋硅藻生物吸附剂的最佳制备条件为:藻粉用量5.0 g/100 m L SA、海藻酸钠浓度20 g·L~(-1)、Ca Cl2质量分数0.5%、交联时间1 h、小球粒径2.8 mm左右.Langmuir等温吸附模型能够较好地描述固定化小球吸附对Pb~(2+)的等温吸附特征,R2为0.9983,最大理论吸附量为833.33 mg·g~(-1).准二级动力学模型能够较好地拟合固定化小球吸附Pb~(2+)的动力学过程,理论平衡吸附量为714.29 mg·g~(-1),与实验所得平衡吸附量706.55 mg·g~(-1)较为接近.固定化小球吸附Pb~(2+)的适宜初始p H值为4~5.Na Cl、Ca(NO3)2、Mg(NO3)2对固定化小球的吸附性能有一定的促进作用.本研究所制固定化海洋硅藻球形吸附材料对Pb~(2+)的吸附容量明显优于大部分研究所报道的固定化生物吸附剂,是一种很有潜力的生物吸附材料.     

受限空间煤尘爆炸毒害气体传播伤害研究

为减少煤矿煤尘爆炸后毒气对人的危害,为煤矿防爆、抑爆和安全评价,以及事故应急救援等提供理论依据,研究了煤尘爆炸后毒气的传播伤害规律。基于质量守恒定律与空气动力学理论,建立煤尘爆炸后风流作用下的毒害气体在受限空间内的数学传播模型,得到巷道内毒气传播的弥散系数,计算出沿爆炸传播方向毒气浓度随距离变化的关系,划分伤害三区并推导出相应的伤害范围计算公式。研究表明:毒气传播的峰值点随风流方向移动,其峰值点浓度逐渐变小。     

基于金相分析技术的煤矿火灾事故调查试验研究

通过对矿井火灾现场特性的分析,根据金属材料的特点,提出利用金相鉴定技术进行矿井火灾事故调查的新方法,试验测定得出高温作用下40号钢和铸铁的金相组织和硬度的动态变化规律。试验结果表明,不同加热温度条件下,40号钢冷却后具有不同的金相组织,其硬度随受热温度的升高迅速下降;在相同加热温度不同受热时间条件下,40号钢的金相组织与硬度变化不明显。铸铁的硬度随加热时间的增加呈下降趋势,其金相组织也有较明显的区别。根据矿井火灾后降温较为缓慢的特点,提出灰口铸铁与白口铸铁相互转变的方向性。因此,矿井火灾事故调查可以对受到火灾破坏的金属构件的金相组织的观察和硬度测定,来"反推"出该部位在火灾时曾受过的最高温度以及持续时间,从而可推断火灾的蔓延情况,为认定起火原因和起火位置提供技术支持。     

铁路危险货物运输风险度量模型

为度量铁路危险货物运输对于人员、环境和财产等方面的危害,在已有危险货物运输风险度量研究的基础上,针对铁路运输的特点,以传统的双指标(概率和后果)技术风险度量模型为框架,构建铁路危险货物运输路径风险度量模型。模型综合考虑人员伤亡、财产损失、运输中断和环境破坏多方面后果,通过有效度量运输风险,为铁路危险货物运输组织优化提供依据。     

基于FDS的天然气集气站分离器喷射火特性仿真研究

天然气集气站火灾事故对集气站安全生产构成严重威胁,研究集气站内关键设备泄漏引发的火灾特性具有重要意义.基于大涡模拟(Large Eddy Simulation)数值方法,针对分离器内介质成分、相态等参数特殊性,采用火灾动力学模拟软件FDS建立集气站卧式气液分离器喷射火模型,对分离器典型泄漏孔径下的喷射火进行数值仿真,研究其火焰几何特性、火场温度及热辐射特性.结果表明,随分离器泄漏孔径的增大,喷射火火焰长度及火焰最大宽度也相应增大.大、小两泄漏孔径的喷射火在充分燃烧区域火焰最高温度分别为1 525 ℃和1 065 ℃.大孔径泄漏的喷射火热辐射值明显高于小孔径,并由于湍流浓度脉动的作用导致热辐射值波动较大,喷射火火焰轴向对称面与相距1.5 m的侧平面上测点热辐射峰值之比与泄漏孔径成正比.根据模拟结果,定量分析了两泄漏孔径下喷射火的危险距离,并提出相应的事故控制措施.     

振动环境下液化气罐内饱和液体状态变化规律研究

液化气在储运过程中存在安全隐患,为分析在运输途中的液化气罐内部压力变化情况,建立液化气罐在受迫振动下的一维数学模型,分析运动过程中饱和液体和气罐的相对运动以及对罐内压力的影响.根据经验公式计算过热液中气泡的生长和消失,并用商业CFD软件CFX模拟在振动过程中罐内介质的状态变化过程,计算由于气泡的生长和消失导致的压力波动.根据这些数据进一步分析罐的振动频率和液体的填充量对汽化速率和压力波动的影响,并得出变化规律.     

石化企业应急救援能力评估研究

随着应急能力建设逐步引起我国各行各业的重视,越来越多的石化企业己经开始加大对应急能力建设工作的力度,以尽可能的减少生产事故所带来的损失。作为石化企业应急能力建设的重要组成部分,石化企业生产事故应急救援能力的评价研究具有十分重要的理论和实践意义。本文首先对石化企业生产及事故特点和石化企业应急能力进行了详细的剖析,初步构建了石化企业生产事故应急能力评价指标体系。进而根据物元可拓性理论,结合石化企业的实际情况,运用可拓评判方法建立石化企业应急救援能力综合评判的模型,给出了评价步骤,最终确定企业的生产事故应急能力等级,从而对企业持续改进应急能力建设提供一定的参考。研究结果表明,该方法可应用于多层次的工程风险评价问题,从而为石化工程项目风险管理提供了一种有效的分析方法。