Rodent sperm analysis in field-based ecological risk assessment: pilot study at Ravenna army ammunition plant,Ravenna, Ohio

Ecological risk assessment (ERA) guidance recommends that field-truthing efforts proceed when modeled hazard quotients (HQs) suggest that toxicological effects are occurring to site receptors. To date, no field methods have been proposed by the regulatory community that can lead to definitive determinations of acceptable or unacceptable risk for birds and mammals, the two terrestrial classes of receptors that are commonly assessed using the HQ method. This paper describes rodent sperm analysis (RSA) as a viable method to be applied in the field at sites with historical contamination. RSA is capable of detecting biological differences that bear on reproduction, a highly regarded toxicological endpoint of concern in USEPA Superfund-type ERAs. The results of RSA's first application at a study site are reported and discussed. The paper also provides the rationale for RSA's efficacy in the context of Superfund and other environmental cleanup programs, where limited time and money are available to determine and evaluate the field condition.     

Thyroid status in juvenile alligators (Alligator mississippiensis) from contaminated and reference sites on Lake Okeechobee,Florida, USA

Exposure to environmental contaminants has been shown to alter normal thyroid function in various wildlife species, including the American alligator (Alligator mississippiensis). Abnormalities in circulating levels of the thyroid hormone thyroxine (T₄) have been reported in juvenile alligators from several contaminated lakes in Florida. To further elucidate these functional thyroid abnormalities, this study examines the structure of thyroids and circulating T₄ concentrations from juvenile alligators collected from three sites of varying contamination on Lake Okeechobee, Florida. The following variables were used to characterize thyroid morphology: epithelial cell height, width and area, percent colloid, and follicle area. These variables were compared among study sites and between genders. No difference was detected in epithelial cell height, epithelial cell area, or follicle area among the sites, whereas significant differences in epithelial cell width (p=0.02) and percent colloid (p=0.008) were found. Animals from the most contaminated site (Belle Glade) had significantly greater epithelial cell widths and significantly less colloid present in their follicles compared to animals from the reference site (West). Gender did not have a significant interaction with site for any variable measured. Thyroxine (T₄) concentrations were elevated in the intermediately contaminated site (Conservation Area 3A) compared to the other sites (p<0.0001). It is proposed that the disruptions seen in Lake Okeechobee alligators are due to disruptions at both the thyroid and extra-thyroidal tissues.     

THE IMPACTS OF CLIMATIC CHANGES FOR WATER RESOURCES OF THE COLORADO AND SACRAMENTO-SAN JOAQUIN RIVER BASINS1

ABSTRACT: A wide variety of regional assessments of the water-related impacts of climatic change have been done over the past two decades, using different methods, approaches, climate models, and assumptions. As part of the Water Sector research for the National Assessment of the Implications of Climatic Variability and Change for the United States, several major summaries have been prepared, looking at the differences and similarities in results among regional research projects. Two such summaries are presented here, for the Colorado River Basin and the Sacramento River Basin. Both of these watersheds are vitally important to the social, economic, and ecological character of their regions; both are large snowmelt-driven basins; both have extensive and complex water management systems in place; and both have had numerous, independent studies done on them. This review analyzes the models, methods, climate assumptions, and conclusions from these studies, and places them in the context of the new climate scenarios developed for the National Assessment. Some significant and consistent impacts have been identified for these basins, across a wide range of potential climate changes. Among the most important is the shift in the timing of runoff that results from changes in snowfall and snowmelt dynamics. This shift has been seen in every regional result across these two basins despite differences in models and climate change assumptions. The implications of these impacts for water management, planning, and policy are discussed.     

Effects of nutrition and temperature on metabolic enzyme activities in larval and juvenile red drum,Sciaenops ocellatus,and lane snapper,Lutjanus synagris

The metabolic enzyme activities were determined in larvae of red drum, Sciaenops ocellatus, and lane snapper, Lutjanus synagris, to determine the effect of temperature and nutrition on metabolic enzyme activities and to evaluate if metabolic enzyme activities are useful in assessing the feeding condition of larval fish. During experiments conducted during the spring of 1990, lactate dehydrogenase (LDH) activities in both red drum and lane snapper were approximately an order of magnitude lower than values typical for adult fish; LDH and citrate synthase (CS) activities increased during early developmental stages, but nutritional effects were apparent. Clear differences (up to 4-fold) between well-fed and starving fish were evident in both LDH and CS activity in red drum. Differences between well-fed and poorly fed larvae were evident until 9 d after hatching. Lane snapper larvae reared at a 25°C had significantly lower LDH activities than larvae reared at 28°C.     

An experiment on the ability of free-ranging turkey vultures (Cathartes aura) to locate carrion by chemical cues

((without abstract.))     

Seasonality of polypide recycling and sexual reproduction in some erect Antarctic bryozoans

The seasonality of polypide cycling has been investigated for three species of erect bryozoans from Antarctica: Isoseculiflustra rubefacta (Kluge, 1914), Nematoflustra flagellata (Waters, 1904) and Himantozoum antarcticum (Calvet, 1905). Approximately ten colonies of each species were collected monthly by SCUBA divers over a 14 mo period during 1992/1993, and the status of each individual zooid was classified as differentiating/regenerating, active (feeding autozooids), degenerate (brown body) or sexually reproductive (ovicells present, or zooid containing a larva). Polypide cycling in all three species was distinctly seasonal. New zooids formed at the growth margin and typically contained actively feeding polypides for ≃9 mo before these polypides degenerated into brown bodies in the austral winter (June). Very few polypides were active in the period from June to August, when water-column food levels were at their lowest; after this period new polypides differentiated. Individual zooids typically underwent a total of five (I. rubefacta and N. flagellata), or at least four (H. antarcticum) complete polypide cycles before becoming senescent. Polypide lifetimes generally became shorter as the age of the zooid increased. Sexual reproduction was also distinctly seasonal in these species, with bands of ovicells or sexually reproductive zooids being formed each year in late summer once a given colony had grown to a threshold size (or age). Larvae were then brooded for ≃10 mo before being released in January/February (N. flagellata) or February/March (H. antarcticum). The seasonal patterns of polypide cycling are related clearly to the variations in food availability, and these species appear to have the longest zooid lifetime (≃5 yr) and the slowest polypide cycling (once per year with polypide lifetimes up to 10 mo) reported for any bryozoan so far. Received: 7 May 1995 / Accepted: 19 November 1997     

Relearning the ABC: taxonomic changes and abundance/biomass relationships in disturbed benthic communities

For marine macrobenthic communities, a shift from higher biomass dominance with increasing levels of disturbance can be determined by the abundance/biomass comparison (ABC) method. This response results from (i) a shift in the proportions of different phyla present in communities, some phyla having larger-bodied species than others, and (ii) a shift in the relative distributions of abundance and biomass among species within the Annelida (specifically Polychaeta) but not within any of the other major phyla (Mollusca, Crustacea, Echinodermata). The shift within polychaetes reflects the substitution of largerbodied by smaller-bodied species, and not a change in the average size of individuals within a species. In most instances the phyletic changes reinforce the trend in species substitutions within the polychaetes, to produce the overall ABC response, but in some cases they may work against each other. Indications of pollution or disturbance detected by this method should be viewed with caution if the species responsible for the polluted configurations are not polychaetes. These observations provide an aid to interpretation of the ABC plots, especially in some situations where they have been deemed to give a false impression of the disturbance status of a community.     

Green Screen or Smokescreen? Hollywood's Messages about Nature and the Environment

This paper examines ideologies about nature and the environment in popular, animated Hollywood films—including The Lorax, Wall-E, and Ice Age 2—through a symptomatic reading. The primary goal of the analysis is to elucidate key omissions in these texts through an assessment of the problematic—defined in this research as an a-priori answer to perceived audience concerns regarding the role of consumerism and corporate culture in environmental problems. Silences in the films revolve around: how environmental problems are defined; what the consequences are; who the responsible parties are; and what potential solutions exist to mitigate them. The significance of the research is underscored by the formation of an increasingly intimate relationship between children, consumer culture, and commercial media in the USA, as well as the increasingly dire information emerging about global environmental issues. This analysis reveals the dual, often conflicting, messages that commercial film provides for its young audiences about pivotal environmental problems and their potential resolution.     

Solid waste characterization and recycling potential for a university campus

Integrated waste management systems are one of the greatest challenges for sustainable development. For these systems to be successful, the first step is to carry out waste characterization studies. In this paper are reported the results of a waste characterization study performed in the Campus Mexicali I of the Autonomous University of Baja California (UABC). The aim of this study was to set the basis for implementation of a recovery, reduction and recycling waste management program at the campus. It was found that the campus Mexicali I produces 1ton of solid wastes per day; more than 65% of these wastes are recyclable or potentially recyclable. These results showed that a program for segregation and recycling is feasible on a University Campus. The study also showed that the local market for recyclable waste, under present conditions - number of recycling companies and amounts of recyclables accepted - can absorb all of these wastes. Some alternatives for the potentially recyclables wastes are discussed. Finally some strategies that could be used to reduce waste at the source are discussed as well.