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81.
Abstract: We examined six historical specimens of the endangered North Atlantic right whale ( Eubalaena glacialis ) using DNA isolated from documented baleen plates from the late nineteenth and early twentieth centuries. Sequences from the mitochondrial DNA (mtDNA) control region from these samples were compared with those from a near-exhaustive survey (269 of approximately 320 individuals) of the remaining right whales in the western North Atlantic Ocean. Our results suggest that there has been only relatively modest change in maternal lineage diversity over the past century in the North Atlantic right whale population. Any significant reduction in genetic variation in the species most likely occurred prior to the late nineteenth century. One historical specimen was from the last documented female capable of propagating one of the maternal lineages in the population today; no females in the existing population have been found to carry this mtDNA haplotype. Analysis of the only specimens from the eastern North Atlantic right whale population ever to be examined revealed that eastern and western North Atlantic right whales may not have been genetically differentiated populations. Loss of gene diversity experienced by North Atlantic right whales over the last century has been modest, and the six decades of protection have been successful in maintaining much of the maternal lineage diversity that was present in the late nineteenth century.  相似文献   
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The Institute of Terrestrial Ecology (ITE) has monitored ecological change in Great Britain (GB) since 1978. The task has been undertaken using a stratified sampling scheme working with a 1 km square as the sample unit. In more recent years, scientific researchers at ITE have been working closely with the policy-makers of the United Kingdom Department of the Environment. The presentation of information to policy advisors and planners was a component within a large project investigating the ecological consequences of land-use change. A simple PC-based decision support system was developed during the project and subsequently has been expanded to produce a marketable product. The system, called the Countryside Information System (CIS), presents and links information at national, regional and thematic levels along with qualifying data describing accuracy and appropriateness of use (i.e., metadata). An integral part of the CIS is the ITE Land Classification, which divides GB into 32 environmental land classes; all 250 000 squares have been classified. The classification allows sampled data to be presented and, as the co-ordinate system is widely used in GB, it allows census datasets to be linked and compared. CIS has been described as a Geographical Information System, but the classification, data held within the system, and the use of metadata to assist in interpretation of results make the system much more decision-support oriented. Indeed, government departments have been involved in directing the development and are now starting to use the system to answer parliamentary questions and formulate, assess and monitor environmental policy. The CIS is an open system, running on a standard PC in Microsoft Windows. Tools for loading and editing new datasets (both sample and census) are incorporated in the suite of programs. The Windows environment and users comments during development have produced a system with an intuitive feel, removing some of the overhead of acquiring specialised technical skills before being able to operate a system. This paper describes the CIS and presents examples of its applications.  相似文献   
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We investigated experimental warming and simulated grazing (clipping) effects on rangeland quality, as indicated by vegetation production and nutritive quality, in winter-grazed meadows and summer-grazed shrublands on the Tibetan Plateau, a rangeland system experiencing climatic and pastoral land use changes. Warming decreased total aboveground net primary productivity (ANPP) by 40 g x m(-2) x yr(-1) at the meadow habitats and decreased palatable ANPP (total ANPP minus non-palatable forb ANPP) by 10 g x m(-2) x yr(-1) at both habitats. The decreased production of the medicinal forb Gentiana straminea and the increased production of the non-palatable forb Stellera chamaejasme with warming also reduced rangeland quality. At the shrubland habitats, warming resulted in less digestible shrubs, whose foliage contains 25% digestible dry matter (DDM), replacing more digestible graminoids, whose foliage contains 60% DDM. This shift from graminoids to shrubs not only results in lower-quality forage, but could also have important consequences for future domestic herd composition. Although warming extended the growing season in non-clipped plots, the reduced rangeland quality due to decreased vegetative production and nutritive quality will likely overwhelm the improved rangeland quality associated with an extended growing season. Grazing maintained or improved rangeland quality by increasing total ANPP by 20-40 g x m(-2) x yr(-1) with no effect on palatable ANPP. Grazing effects on forage nutritive quality, as measured by foliar nitrogen and carbon content and by shifts in plant group ANPP, resulted in improved forage quality. Grazing extended the growing season at both habitats, and it advanced the growing season at the meadows. Synergistic interactions between warming and grazing were present, such that grazing mediated the warming-induced declines in vegetation production and nutritive quality. Moreover, combined treatment effects were nonadditive, suggesting that we cannot predict the combined effect of global changes and human activities from single-factor studies. Our findings suggest that the rangelands on the Tibetan Plateau, and the pastoralists who depend on them, may be vulnerable to future climate changes. Grazing can mitigate the negative warming effects on rangeland quality. For example, grazing management may be an important tool to keep warming-induced shrub expansion in check. Moreover, flexible and opportunistic grazing management will be required in a warmer future.  相似文献   
86.
The Institute of Terrestrial Ecology (ITE) has been studying land use and the effects of land use on ecology for two decades. A series of national field surveys have been undertaken by the Land Use Section of ITE since 1978, the most recent being Countryside Survey 1990 (CS1990). The three-year project brought together field survey and remote sensing data which were analyzed using Geographical Information Systems (GIS). National and regional land-cover patterns were described and changes estimated.The data collected by the field survey part of CS1990 recorded stratified samples based on a land classification. Thematic maps for surveyed 1-km squares covered physiography, agriculture and semi-natural vegetation, forestry, structures and boundaries. The same sites were surveyed in 1984 and 1990 with 14 000 digital maps produced describing both years. GIS was used to generate stock figures for each year, and overlay allowed change between survey dates to be estimated.GIS was used to compare data collected from both field survey and satellite imagery so that both sets of information could be qualified when expressed as national figures.This paper describes the historical development of the ITE Land Classification, examines the way in which data were collected for surveys, with particular reference to Countryside Survey 1990, and shows how satellite and field survey data can be linked through GIS.  相似文献   
87.
Sea water contaminated with diluted radioactive effluent originating from the Sellafield reprocessing plant inundates saltmarshes in the Esk estuary in west Cumbria (UK). Much of the radioactivity on the saltmarsh vegetation was found to be associated with a surface deposit of silt, brought onto the area during tidal inundation. Large temporal variations in the radionuclide concentrations of the vegetation were found, these being due probably to seasonal fluctuation in the extent of silt deposition.The grazing habits of sheep were studied on one of these saltmarshes and the consequent ingestion of radionuclides by the sheep was estimated. The sheep ingested different quantities of radionuclides according to time of year as a result of a combination of two factors; the considerable temporal variation in the concentration of radionuclides on the saltmarsh vegetation and the seasonal grazing behaviour of the sheep.  相似文献   
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The effect of repeat testing in maternal serum multiple marker screening for Down's syndrome was estimated using samples stored in an antenatal serum bank. Human chorionic gonadotropin (hCG) and unconjugated oestriol (uE3) levels were determined in 142 pairs of routinely collected samples which had already been tested for alpha-fetoprotein (AFP). For each marker, about two-thirds of the pairs of values were within 20 per cent of each other and most were within 40 per cent. A multivariate Gaussian model was used to estimate the detection and false-positive rates for different repeat testing policies. A policy of repeat testing those with a high risk of a Down's syndrome term pregnancy given age and marker levels would reduce the false-positive rate but there would also be a reduction in the detection rate. For example, using all three markers and a 1 in 250 cut-off risk, the estimated false-positive rate would fall from 5·3 to 3·8 per cent but the detection rate would decrease from 58 to 55 per cent. A policy of repeating those with either high or borderline risks would produce a modest improvement in screening efficiency. Repeating the 11 per cent with a risk exceeding 1 in 500 yields an estimated false-positive rate of 5·0 per cent and a detection rate of 60 per cent. A policy of selective repeat testing is not recommended as it would not substantially improve screening efficiency. Nonetheless, if a repeat test has been performed, the parameters given in this paper will enable an unbiased estimate of the Down's syndrome risk to be calculated for individual women.  相似文献   
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