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971.
A simplified method is described for processing both direct preparations and long-term cultures from the same fragment of chorionic villi. Enzyme separation of the outer trophoblast layers (used for direct preparations) from the inner mesenchymal core (used to initiate long-term cultures) facilitates the utilization of the same fragments for the two procedures, without jeopardizing the success of either method. This has proved useful in cases where the sample was so small that only one method of chromosome preparation may have been possible using other techniques. 相似文献
972.
D. J. H. Brock 《黑龙江环境通报》1994,14(13):1243-1252
A number of different models of CF carrier screening have now been tested in pilot trials. Apart from opportunistic and cascade testing (which are strictly speaking not true forms of screening), the major programmes have been directed either to young adults in primary care or to pregnant women in antenatal clinics. Only in the latter form of screening has sufficient data been collected to allow conclusions to be reached on the optimum mode of delivery. It seems very probable that when CF carrier screening passes into routine service, it will be the antenatal couple model that is used. 相似文献
973.
研究海水入侵污水放流系统及其清除特性有助于系统优化设计和运行保护。运用水力学一般原理推导了清除流量和入侵临界流量的估算公式,并根据数学模型模拟结果讨论了部分系统参数对清除和入侵的影响,指出系统沿程阻力的增加及扩散器主管截面逐渐减小对减小清除流量作用不大,而立管在主管底部与主管联结与在顶部联结相比一般可减小清除流量10%以上。系统清除流量和人侵临界流量与立管个数成正比。还介绍了鸭嘴阀,文丘里喉管等几种限制入侵和帮助清除的技术措施。介绍的方法与结果可供工程实际应用。 相似文献
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980.
Nicolas Lamouroux Hervé Pella Ton H. Snelder Eric Sauquet Jérome Lejot Ude Shankar 《Journal of the American Water Resources Association》2014,50(1):1-13
Spatially comprehensive estimates of the physical characteristics of river segments over large areas are required in many large‐scale analyses of river systems and for the management of multiple basins. Remote sensing and modeling are often used to estimate river characteristics over large areas, but the uncertainties associated with these estimates and their dependence on the physical characteristics of the segments and their catchments are seldom quantified. Using test data with varying degrees of independence, we derived analytical models of the uncertainty associated with estimates of upstream catchment area (CA), segment slope, and mean annual discharge for all river segments of a digital representation of the hydrographic network of France. Although there were strong relationships between our test data and estimates at the scale of France, there were also large relative local uncertainties, which varied with the physical characteristics of the segments and their catchments. Discharge and CA were relatively uncertain where discharge was low and catchments were small. Discharge uncertainty also increased in catchments with large rainfall events and low minimum temperature. The uncertainty of segment slope was strongly related to segment length. Our uncertainty models were consistent across large regions of France, suggesting some degree of generality. Their analytical formulation should facilitate their use in large‐scale ecological studies and simulation models. 相似文献