Biochar produced from anaerobically digested fiber reduces phosphorus in dairy lagoons

This study evaluated the use of biochar produced from anaerobic digester dairy fiber (ADF) to sequester phosphorus (P) from dairy lagoons. The ADF was collected from a plugged flow digester, air-dried to <8% water content, and pelletized. Biochar was produced by slow pyrolysis in a barrel retort. The potential of biochar to reduce P in the anaerobic digester effluent (ADE) was assessed in small-scale filter systems through which the effluent was circulated. Biochar sequestered an average of 381 mg L P from the ADE, and 4 g L ADF was captured as a coating on the biochar. There was an increase of total (1.9 g kg), Olsen (763 mg kg), and water-extractable P (914 mg kg) bound to the biochar after 15 d of filtration. This accounted for a recovery of 32% of the P in the ADE. The recovered P on the biochar was analyzed using P nuclear magnetic resonance for P speciation, which confirmed the recovery of inorganic orthophosphate after liquid extraction of the biochar and the presence of inextractable Ca-P in the solid state. The inorganic phosphate was sequestered on the biochar through physical and weak chemical bonding. Results indicate that biochar could be a beneficial component to P reduction in the dairy system.     

Morphology Characteristics of Southern Appalachian Wilderness Streams1

Zink, Jason M., Gregory D. Jennings, and G. Alexander Price, 2012. Morphology Characteristics of Southern Appalachian Wilderness Streams. Journal of the American Water Resources Association (JAWRA) 48(4): 762‐773. DOI: 10.1111/j.1752‐1688.2012.00647.x Abstract: Watersheds without urbanization or impacts from logging are rare in the southern Appalachian Mountains. The Joyce Kilmer/Slickrock Wilderness of North Carolina and Tennessee contains 24 km² of old‐growth forest, with the balance of the wilderness in a mature second‐growth forest. The watersheds of Little Santeetlah and Slickrock Creek are located within the wilderness. Morphological information, including channel dimensions and longitudinal profiles, was gathered from 14 alluvial stream reaches in these watersheds. The study sites had drainage areas from 0.25 to 41.6 km² and stream slopes from 0.014 to 0.104 m/m. Bankfull cross‐section dimensions of the study stream reaches were strongly correlated to drainage area across the observed range of slopes and bed morphology. Cross‐section area and width relationships for the streams in this study did not differ significantly from regional curves for the mountain physiographic region of North Carolina. Observations of these reaches did not suggest a definitive rule regarding the proportion of steps and riffles in streams. Pools occupied greater than 50% of the length in all stream reaches with slopes less than 0.07 m/m. Significant correlation existed between step height ratio and slope, suggesting that step height can be approximated as the product of channel width and slope. Riffle length and riffle slope ratios were also significantly correlated with slope, though pool spacing was not.     

Nutrient Removal and Loading Rate Analysis of Louisiana Forested Wetlands Assimilating Treated Municipal Effluent

The relationship between nutrient removal and loading rate was examined using data from five forested wetlands in Louisiana that have received secondarily treated effluent from 3 to 60 years. Loading rates ranged from 0.65 to 26.80 g/m²/yr for total nitrogen and 0.18 to 8.96 g/m²/yr for total phosphorus. At loading rates below 20 g/m²/yr, total nitrogen concentrations in surface waters of Louisiana forested wetlands were reduced to background concentrations (i.e., ≤3 mg/l). Similarly, at loading rates below 2 g/m²/yr, total phosphorus concentrations were also generally reduced to background concentrations (i.e., ≤1 mg/l). These data demonstrate that freshwater forested wetlands can reduce nutrient concentrations in treated effluent to background concentrations present in relatively undisturbed wetlands. An understanding of the relationship between loading rates and nutrient removal in natural wetlands is important, particularly in Louisiana where discharges of fresh water are being used in ecosystem restoration.     

Effects of sublethal application of Deepwater Horizon oil to bird eggs on embryonic heart and metabolic rate

Following large crude oil spills, oil from feathers of brooding birds and oiled nesting material can transfer to eggs, resulting in reduced embryonic viability for heavily oiled eggs. Eggs may also be subjected to trace or light oiling, but functional teratogenic effects from sublethal crude oil exposure have not been examined. We assessed whether sublethal application of weathered Deepwater Horizon crude oil to the eggshell surface alters heart rate and metabolic rate in Zebra Finch (Taeniopygia guttata) embryos. We first determined sublethal applications with a dosing experiment. Embryo viability for eggs exposed to 5 μL or more of crude oil decreased significantly. We conducted a second experiment to measure heart rate and metabolic rate (CO₂ production) 5 and 9 d after 1 sublethal application of crude oil to eggshells on day 3 of incubation. One application of 1.0 or 2.5 µL of crude oil reduced embryonic heart rate and metabolic rate on day 12 of incubation. Using unfertilized eggs, we measured the transfer of polycyclic aromatic hydrocarbons (PAHs) from the eggshell surface to egg contents 9 d after a single application of sublethal crude oil. Our results suggest avian eggs externally exposed to small amounts of crude oil may exhibit protracted embryonic development and impaired postnatal cardiac performance.     

Iodine sequestration using delafossites and layered hydroxides

The objective of this document is to report on the early success for sequestering ¹²⁹I. Sorption coefficients (K _d) for I⁻ and IO₃ ⁻ onto delafossites, spinels and layered metal hydroxides were measured and compared for their applicability to sequester ¹²⁹I. The studies were performed using a dilute fluid composition representative of groundwater indigenous to the Yucca mountain area. Delafossites generally exhibited relatively poor sorption coefficients (<35 mL/g). In contrast, the composition of the layered hydroxides significantly affects their ability to sorb I. Cu/Al and Cu/Cr layered hydroxide samples exhibit K _d’s greater than 1000 mL/g for both I⁻ and IO₃ ⁻.     

Deformation patterns in WorldSID 50th percentile dummy instrumented with IR-TRACC and RibEye™ in different loading configurations

Objective: The purpose of this study was to investigate the effect of different loading configurations on the WorldSID 50th percentile male dummy instrumented either with the Infra-Red Telescoping Rod for the Assessment of Chest Compression (IR-TRACC) or the RibEye? rib deflection measurement system.

Methods: The optical sensors of the RibEye system were used to capture the multipoint deformation of the dummy at frontal and rearward off-center locations in addition to the center of the rib location. The experimental setup consisted of 2 types of loadings: Low severity and high severity. Low-severity loading was performed by deploying a fixture-mounted side airbag on the dummy and high-severity loading was achieved by deploying a driver front airbag mounted in a similar fashion. The low-severity condition aimed at deforming the dummy’s ribs locally at off-center locations where the RibEye light emitting diodes (LEDs) were positioned to capture the deformations at those locations. The high-severity condition aimed at loading the dummy at high speed in lateral and oblique directions similar to what is experienced by dummies in side impacts.

Results: In the low-severity tests, the peak deflections, in terms of length change, were approximately 15–20?mm, whereas for the high-severity cases the peak deflections were in the range of 30–40?mm for both IR-TRACC and RibEye cases.

Conclusions: For similar physical insults, dummies with the IR-TRACC and RibEye systems showed varying results for both length changes and the shoulder forces depending on the severity and direction of loading. Under purely lateral loading, the mid-length changes with the RibEye and the 1D IR-TRACC were comparable. In the oblique loading conditions, more differences were seen with the 2 systems depending on the impact direction. The shoulder forces consistently differed between the 2 systems. In the frontal oblique low-severity cases, the ribs pivoted along the spine end and the length change was not found to be a suitable parameter to quantify rib deformation in such loading scenarios.     

The armoured dissorophid Cacops from the Early Permian of Oklahoma and the exploitation of the terrestrial realm by amphibians

Cacops, one of the most distinctive Paleozoic amphibians, is part of a clade of dissorophoid temnospondyls that diversified in the equatorial region of Pangea during the Late Carboniferous and Early Permian, persisting into the Late Permian in Central Russia and China. Dissorophids were a successful group of fully terrestrial, often spectacularly armoured predators, the only amphibians apparently able to coexist with amniotes when the latter started to dominate terrestrial ecosystems. In this paper, we describe excellent new skulls from the Early Permian of Oklahoma attributed to Cacops, Cacops morrisi sp. nov. and provide for the first time detailed information about this iconic dissorophid. These specimens show anatomical and ontogenetic features that will impact on future studies on the evolution of terrestriality in tetrapods. For example, the large, posteriorly closed tympanic embayment has fine striations on an otherwise smooth surface, documenting the oldest known clear evidence for the presence of a tympanic membrane in the fossil record, a structure that is used for hearing airborne sound in extant tetrapods. The skull of C. morrisi also has several features associated with predatory behaviour, indicating that this dissorophid may have been one of the top terrestrial predators of its time.     

Ascorbic acid, β-carotene,sugars, phenols,and heavy metals in sweet potatoes grown in soil fertilized with municipal sewage sludge

Municipal sewage sludge (MSS) used for land farming typically contains heavy metals that might impact crop quality and human health. A completely randomized experimental design with three treatments (six replicates each) was used to monitor the impact of mixing native soil with MSS or yard waste (YW) mixed with MSS (YW +MSS) on: i) sweet potato yield and quality; ii) concentration of seven heavy metals (Cd, Cr, Mo, Cu, Zn, Pb, and Ni) in sweet potato plant parts (edible roots, leaves, stem, and feeder roots); and iii) concentrations of ascorbic acid, total phenols, free sugars, and β-carotene in sweet potato edible roots at harvest. Soil samples were collected and analyzed for total and extractable metals using two extraction procedures, concentrated nitric acid (to extract total metals from soil) as well as CaCl₂ solution (to extract soluble metals in soil that are available to plants), respectively. Elemental analyses were performed using inductively coupled plasma mass spectrometry (ICP-MS). Overall, plant available metals were greater in soils amended with MSS compared to control plots. Concentration of Pb was greater in YW than MSS amendments. Total concentrations of Pb, Ni, and Cr were greater in plants grown in MSS+YW treatments compared to control plants. MSS+YW treatments increased sweet potato yield, ascorbic acid, soluble sugars, and phenols in edible roots by 53, 28, 27, and 48%, respectively compared to plants grown in native soil. B-carotene concentration (157.5 μg g^?1 fresh weight) was greater in the roots of plants grown in MSS compared to roots of plants grown in MSS+YW treatments (99.9 μg g^?1 fresh weight). Concentration of heavy metals in MSS-amended soil and in sweet potato roots were below their respective permissible limits.     

Understanding the net effects of pesticides on amphibian trematode infections

Anthropogenic factors can have simultaneous positive and negative effects on parasite transmission, and thus it is important to quantify their net effects on disease risk. Net effects will be a product of changes in the survival and traits (e.g., susceptibility, infectivity) of both hosts and parasites. In separate laboratory experiments, we exposed cercariae of the trematode Echinostoma trivolvis, and its first and second intermediate hosts, snails (Planorbella trivolvis) and green frog tadpoles (Rana clamitans), respectively, to one of four common pesticides (atrazine, glyphosate, carbaryl, and malathion) at standardized, ecologically relevant concentrations (201.0, 3700.0, 33.5, and 9.6 microg/L, respectively). We measured effects of pesticide exposure on six mechanisms important to this host-parasite interaction: (1) survival of E. trivolvis cercariae over 26 hours, (2) tadpole survival over two weeks, (3) snail survival over four weeks, (4) snail growth and fecundity, (5) cercarial infectivity, and (6) tadpole susceptibility to a fixed number of cercariae. Pesticides, in general, caused significantly greater mortality of E. trivolvis cercariae than did control treatments, but atrazine was the lone chemical to significantly reduce cercarial survival (LC50 value = 267 mg/L) and then only at concentrations greater than commonly found in aquatic ecosystems (> or =200 microg/L). None of the pesticides significantly enhanced E. trivolvis virulence, decreased tadpole survival, or reduced snail survival, growth, or fecundity. Sublethal exposure of the cercariae to the pesticides (4 h) did not significantly affect trematode encystment in R. clamitans. In contrast, sublethal exposure of R. clamitans to each of the four pesticides increased their susceptibility as measured by the percentage of cercariae that encysted. The reduction in exposure to trematodes due to pesticide-induced cercarial mortality (a density-mediated effect) was smaller than the pesticide-induced increase in amphibian susceptibility (a trait-mediated effect), suggesting that the net effect of exposure to environmentally realistic levels of pesticides will be to elevate amphibian trematode infections. These findings highlight the importance of elucidating the lethal and sublethal effects of anthropogenic factors on both hosts and parasites to understand the mechanisms underlying changes in parasite transmission and virulence, an approach that is especially needed for amphibians, a taxon experiencing global disease-related declines.