Assessment of the natural attenuation of chlorinated ethenes in an anaerobic contaminated aquifer in the Bitterfeld/Wolfen area using stable isotope techniques, microcosm studies and molecular biomarkers

The in situ degradation of chlorinated ethenes was assessed in an anaerobic aquifer using stable isotope fractionation approaches, microcosm studies and taxon specific detection of specific dehalogenating groups of bacteria. The aquifer in the Bitterfeld/Wolfen region in Germany contained all chlorinated ethenes, benzene and toluene as contaminants. The concentrations and isotope composition of the chlorinated ethenes indicated biodegradation of the contaminants. Microcosm studies confirmed the presence of in situ microbial communities capable of the complete dechlorination of tetrachloroethene. Taxon specific investigation of the microbial communities indicated the presence of various potential dechlorinating organisms including Dehalococcoides, Desulfuromonas, Desulfitobacterium and Dehalobacter. The integrated approach, using metabolite spectra, molecular marker analysis and isotope studies, provided several lines of evidence for natural attenuation of the chlorinated ethenes.     

Tree and stand growth of mature Norway spruce and European beech under long-term ozone fumigation

In a 50- to 70-year-old mixed stand of Norway spruce (Picea abies (L.) Karst.) and European beech (Fagus sylvatica L.) in Germany, tree cohorts have been exposed to double ambient ozone (2×O₃) from 2000 through 2007 and can be compared with trees in the same stand under the ambient ozone regime (1×O₃). Annual diameter growth, allocation pattern, stem form, and stem volume were quantified at the individual tree and stand level. Ozone fumigation induced a shift in the resource allocation into height growth at the expense of diameter growth. This change in allometry leads to rather cone-shaped stem forms and reduced stem stability in the case of spruce, and even neiloidal stem shapes in the case of beech. Neglect of such ozone-induced changes in stem shape may lead to a flawed estimation of volume growth. On the stand level, 2×O₃ caused, on average, a decrease of 10.2 m³ ha⁻¹ yr⁻¹ in European beech.     

The influence of natural stressors on the toxicity of nickel to <Emphasis Type="Italic">Daphnia magna</Emphasis>

Global warming has become a source of awareness regarding the potential deleterious effects of extreme abiotic factors (e.g., temperature, dissolved oxygen (DO) levels) and also their influence on chemicals toxicity. In this work, we studied the combined effects of nickel and temperature (low and high levels) and nickel and low levels of DO to Daphnia magna, and concentration addition and independent action concepts as well as their deviations for synergism/antagonism, dose ratio and dose level dependency, were applied to survival and feeding rate data. Nickel single exposure showed an LC₅₀ value for 48 h of 7.36 mg l⁻¹ and an EC₅₀ value for feeding impairment at 2.41 mg l⁻¹. In the acute exposures to high and low temperatures, 50% of mortality was observed, respectively, at 30.7°C and 4.2°C whereas 50% reduction on the feeding activity was recorded at 22.6°C and 16.0°C. Relatively to low DO levels, a LC₅₀ value for 48 h of 0.5 mg l⁻¹ was obtained; feeding activity EC₅₀ value was 2 mg l⁻¹. On acute combined experiments, antagonism was observed for the combination of nickel and extreme temperatures, whereas a synergistic behaviour was observed in the combined exposure of nickel and low DO levels. At sublethal levels, nickel showed to be the main inducer of toxicity at high and low temperatures but not at low levels of dissolved oxygen. Toxicokinetics and toxicodynamics modelling studies should be made in the future to understand the toxicological pathways involved on complex combinations of stressors and to validate any conclusions.     

Impact of clouds and aerosols on ozone production in Southeast Texas

A radiative transfer model and photochemical box model are used to examine the effects of clouds and aerosols on actinic flux and photolysis rates, and the impacts of changes in photolysis rates on ozone production and destruction rates in a polluted urban environment like Houston, Texas. During the TexAQS-II Radical and Aerosol Measurement Project the combined cloud and aerosol effects reduced j(NO₂) photolysis frequencies by nominally 17%, while aerosols reduced j(NO₂) by 3% on six clear sky days. Reductions in actinic flux due to attenuation by clouds and aerosols correspond to reduced net ozone formation rates with a nearly one-to-one relationship. The overall reduction in the net ozone production rate due to reductions in photolysis rates by clouds and aerosols was approximately 8 ppbv h^?1.     

Persistent organochlorine pesticides in human milk samples from Australia

Human milk has been used as a surrogate for the assessment of body burden and exposure to persistent lipophilic organochlorine pesticides (OCPs). With the exception of restricted use of mirex, the use of persistent, lipophilic OCPs has been banned in Australia since the 1980s. The decline of human body burden of OCPs following their ban in many industrialised countries is well recorded worldwide from the 1970s until the 1990s though little is known on whether these trends are continuing. In this study, 157 human milk samples collected during 2002 and 2003 as well as 24 samples collected in 1993 were analysed as 20 regional pools for 17 OCPs. OCPs were detected in all pooled human milk samples from 2002/03 typically with highest concentrations of p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) (mean+/-standard deviation; median concentration 311+/-174; 279 ng g(-1) lipid) followed by beta-hexachlorocyclohexane (beta-HCH) (80+/-173; 21 ng g(-1) lipid). Other OCPs consistently detected included dieldrin (16+/-6; 17 ng g(-1) lipid), hexachlorobenzene (HCB) (18+/-16; 14 ng g(-1) lipid), transnonachlor (11+/-5; 9 ng g(-1) lipid) and p,p'-dichlorodiphenyltrichloroethane (p,p'-DDT) (9+/-6; 7 ng g(-1) lipid). The results from this study indicated that following a substantial decline of OCP concentrations from the early 1980s to the 1990s, little decline could be observed since then. Furthermore the use of multivariate statistics indicated some regional trends with slightly higher levels of the broadly used insecticides DDT and HCH in both historic and recent samples from Melbourne, whereas, sample pools collected from mothers that lived in rural Queensland and New South Wales as well as Adelaide and Sydney showed comparatively higher levels of heptachlor and dieldrin - both of which have been used for termite treatment. These results indicate that even 20 years after the discontinuation of usage, historical use of OCPs rather than exposure via global transport of OCPs is responsible for continuous low exposure in Australia.     

Evaluation of performance reference compounds in polyethylene-based passive air samplers

Polyethylene-based passive air samplers (PSDs) were loaded with performance reference compounds (PRCs) and deployed in a wind tunnel to examine the effects of wind speed on sampler performance. PRCs could be loaded reproducibly into PSDs, with coefficients of variation only exceeding 20% for the more volatile compounds. When PSDs were exposed to low (0.5-1.5 m s-1) and high (3.5-4.5 m s-1) wind speeds, PRC loss rate constants generally increased with increasing wind speed and decreased with increasing sampler/air partition coefficients. PSD-based air concentrations calculated using PRC loss rate constants and sampler/air partition coefficients and air concentrations measured using active samplers compared closely. PRCs can be used to account for the effect of differences in wind speeds on sampler performance and measure air concentrations with reasonable accuracy.     

Sea-level rise impacts on Africa and the effects of mitigation and adaptation: an application of DIVA

This paper assesses sea-level rise impacts on Africa at continental and national scales including the benefits of mitigation and of applying adaptation measures, considering four scenarios of global mean sea-level rises from 64 to 126 cm in the period of 1995–2100. We find that in 2100, 16–27 million people are expected to be flooded per year, and annual damage costs range between US5 and US 5 and US 9 billion, if no adaptation takes place. Mitigation reduces impacts by 11–36%. Adaptation in the form of building dikes to protect against coastal flooding and nourishing beaches to protect against coastal erosion reduces the number of people flooded by two orders of magnitude and cuts damage costs in half by 2100. Following such a protection strategy would require substantial investment. First, Africa’s current adaptation deficit with respect to coastal flooding would need to be addressed. DIVA suggests that a capital investment of US300 billion is required to build dikes adapted to the current surge regime and US 300 billion is required to build dikes adapted to the current surge regime and US 3 billion per year for maintenance. In addition, between US2 and US 2 and US 6 billion per year needs to be spent on protecting against future sea-level rise and socio-economic development by 2100. This suggests that protection is not effective from a monetary perspective but may still be desirable when also taking into account the avoided social impact. We conclude that this issue requires further investigation including sub-national scale studies that look at impacts and adaptation in conjunction with the development agenda and consider a wider range of adaptation options and strategies.     

Field investigation of the quality of fresh and aged leachates from selected landfills receiving e-waste in an arid climate

The management of electronic waste (e-waste) is a serious problem worldwide and much of it is landfilled. A survey of four selected landfills in an arid region of South Australia was conducted to determine the proportion of e-waste in municipal waste and the properties of each landfill site. Leachate and groundwater samples were collected upgradient and downgradient of the landfills for analysis of polybrominated diphenyl ethers (PBDEs) and 14 metals and metalloids, including Al, As, Ba, Be, Cd, Co, Cr, Cu, Fe, Ni, Pb, Sb, V and Zn. Our data demonstrate that the selected landfills in South Australia continue to receive municipal waste containing in excess of 6%, or 25,000 tonnes per year, of e-waste. The leachates and groundwater collected from the landfills contained significantly elevated concentrations of Pb with the highest concentration in groundwater of 38 μg/l, almost four times higher than the Australian drinking water guideline of 10 μg/l. The presence of PBDEs was detected in both leachate and groundwater samples. Total PBDEs values of 2.13–59.75 ng/l in leachate samples were 10 times higher than in groundwater samples, which recorded a range of 0.41–6.53 ng/l at all sites. Moreover, the concentrations of metals and metalloids in sampled groundwater contained elevated levels of Al, As, Fe, Ni and Pb that exceeded Australian drinking water guideline values. For these reasons potential leaching of these contaminants is of concern and while difficult to attribute elevated contaminant levels to e-waste, we do not recommend continued disposal of e-waste in old landfills that were not originally designed to contain leachates. The survey also revealed temporal variation in the electrical conductivity and concentrations of As, Cd and Pb present in leachates of landfills in arid Mediterranean climates. These results are consistent with the marked variations in rainfall patterns observed for such climates. The solute concentration (EC and other ions including As, Cd and Pb) declines in the leachates during wet winter months (June to September), in contrast to tropical countries where such changes are observed during wet summer months.