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991.
Diana W. Bianchi MD Gretchen K. Zickwolf Melissa C. Yih Alan F. Flint Ossie H. Geifman Marlena S. Erikson John M. Williams 《黑龙江环境通报》1993,13(4):293-300
Fetal nucleated erythrocytes (NRBC) in maternal blood are a non-invasive source of fetal DNA for prenatal genetic screening. We compared the effectiveness of three monoclonal antibodies for the separation of fetal cells from maternal blood by flow sorting. Mononuclear blood cells from 49 healthy pregnant women were incubated with antibody to CD 71, CD 36, and/or glycophorin A (GPA), employed singly or in combination with each other. These monoclonal antibodies recognize surface antigens on haematopoietic precursor cells. Successful isolation of fetal cells was defined as detection of Y chromosomal sequences in maternal blood from women carrying male fetuses, with absence of Y sequences when female fetuses were carried. Thus, gender prediction accuracy was used as a measure of fetal cell separation. Using anti-CD 71 to isolate fetal cells, gender prediction was 57 per cent correct; with anti-CD 36, it was 88 per cent correct. Anti-GPA, an erythrocyte-specific antigen, used alone or in combination with anti-CD 71 or 36, improved gender prediction to 100 per cent. We conclude that antibody to GPA improves the retrieval of fetal NRBC from maternal blood, permitting genetic analysis by the polymerase chain reaction. 相似文献
992.
A simple,cost-effective multispecies toxicity test using organisms with a cosmopolitan distribution 总被引:4,自引:0,他引:4
John Cairns Jr. James R. Pratt B. R. Niederlehner P. V. McCormick 《Environmental monitoring and assessment》1986,6(3):207-220
Difficulties in making accurate, ecosystem-level predictions of environmental effects of chemicals, mixtures, and effluents based solely on the results of tests on single species have necessitated the development of more environmentally realistic, predictive testing methods. This paper describes a multispecies, community-level toxicity test based on the colonization of artificial substrates by microbial species. Tests examined the colonization of initially barren polyurethane foam artificial substrates by Protozoa from a species source colonized in a natural system. Differences in colonization were examined in microecosystems amended with low levels of cadmium, a very toxic heavy metal, and TFM, an organic biocide used against larval sea lamprey. Tests examined differences in colonization over 28 days. For cadmium, effect levels were estimated to be near 1 g 1–1, in the low range of effect levels determined from chronic single species tests. For TFM, effect levels were estimated to be between 1 and 10 ppm, overlapping the concentrations used in environmental applications. The colonization response, which depends on naked microbial cells reproducing and migrating through toxicant amended water to new substrates, is very sensitive. Tests based on colonization can be adapted to use species from a target receiving system or can use species from a designated natural source. Field validation of these tests can employ nearly identical methods to those used in laboratory studies to assess the accuracy of predictions based on test system data. 相似文献
993.
Conservation scientists are increasingly interested in the question of how extinction prunes the tree of life. This question is particularly important for Australian freshwater fishes because there is a broad mix of ∼300 old and young species, many of which are severely threatened. We used a complete species-level phylogeny of Australian freshwater fishes to examine phylogenetic nonrandomness of extinction risk. We computed the potential loss of phylogenetic diversity by simulating extinction across the tree under a pattern weighted based on International Union for Conservation of Nature extinction risk category and compared this loss to projected diversity loss under a random null model of extinction. Finally, we calculated EDGE (evolutionary distinctiveness, global endangerment) scores for 251 freshwater and 60 brackish species and compiled a list of high-priority species for conservation actions based on their extinction risk and evolutionary uniqueness. Extinction risk was not random and was clustered in both diversity cradles (recently diversifying, species-rich clades, such as Galaxiidae and Percichthyidae) and museums (older, species-poor groups, such as freshwater chondrichthyans). Clustered extinction made little difference to the average expected loss of phylogenetic diversity. However, the upper bound of loss was higher under a selective model of extinction, particularly when the counts of species lost were low. Thus, the loss of highly threatened species would diminish the tree of life more than a null model of randomly distributed extinction. High priority species included both widely recognized and charismatic ones, such as the Queensland lungfish (Neoceratodus forsteri), river sharks, and freshwater sawfishes, and lesser-known species that receive less public attention, including the salamanderfish (Lepidogalaxias salamandroides), cave gudgeons, and many galaxiids, rainbowfishes, and pygmy perches. 相似文献
994.
Mughal Nafessa Kashif Maryam Arif Asma Guerrero John William Grimaldo Nabua Wilson C. Niedbała Gniewko 《Environmental science and pollution research international》2021,28(46):65116-65126
Environmental Science and Pollution Research - This study aims to re-examine the impacts of monetary and fiscal policy on environmental quality in ASEAN countries from 1990 to 2019. We utilized the... 相似文献
995.
Tolis Evangelos I. Karanotas Tilemachos Svolakis Grigoris Panaras George Bartzis John G. 《Environmental science and pollution research international》2021,28(37):51232-51241
Environmental Science and Pollution Research - Despite that commuters spend only 5.5% of their time in cabin vehicles, their exposure to harmful air pollutants, originated from the vehicle itself,... 相似文献
996.
John E. Hay 《Sustainability Science》2013,8(3):309-326
The intent of this paper is to place the concepts of exposure, vulnerability, resilience and risk in the context of the consequences of global change for the sustainable development of small island developing states (SIDS). Many such states face a number of global climate change risks, such as an increase in the proportion of more intense storms, along with other global change threats that include energy security and costs. All these threats come on top of local development threats, such as increased run-off, often with increasing levels of contaminants due to unsustainable agricultural and industrial practices. When taken together, the resulting pressures on islands and their communities lead to significant increases in vulnerability to change due to reduced resilience to these changes. Vulnerability is also increasing as a result of contemporary processes that heighten the exposure of material and other assets. The capacity to address hazard risk also influences vulnerability. This includes the level of awareness of coastal hazards and exposure, and access to critical life support infrastructure, especially for people living in hazard-prone areas. Vulnerability and resilience are considered to be important integrating concepts when managing the local consequences of global changes. There are many initiatives that will help reduce the vulnerability and enhance the resilience of SIDS to such changes. These include improving risk knowledge and coastal resource and land use management, while also strengthening socio-economic systems and livelihoods. In this way, managing global change can be closely aligned with local development and humanitarian processes, thereby enhancing the overall sustainability of development processes and outcomes. 相似文献
997.
John E. Tilton 《Natural resources forum》1989,13(3):198-203
In the early years after World War II the developed countries, and in particular the USA, acounted for a large portion of world metal consumption. Since that time a shift in consumption has occurred favouring the industrialized states of Western Europe, the centrally planned economies of Eastern Europe, and more recently Japan and the other Pacific Rim countries of Asia (PRA). After assessing the magnitude of the shift in metal consumption toward the PRA countries, this paper focuses on differences in economic growth, the nature of trade, and other factors responsible. Finally, the implications of the shift are considered for the nature of metal trade, the competitiveness of metal exporting countries, the structure of international metal markets, and the future availability of metal supplies. 相似文献
998.
999.
Environment as the Stage for Economic Actors 总被引:6,自引:0,他引:6
John E Coulter Shi Lei Samantha Jenkins 《中国人口.资源与环境(英文版)》2007,5(1):3-8
The relative importance of economics and environment in debate may soon be reversed due to the influence of three fac- tors. Firstly, in the global economy it is hard to hide the unwanted products of economic processes. Secondly, huge advances in sci- ence will reduce the imperfect knowledge of markets, making some monitoring and analyzing tools show the design of sensible and equitable livelihood in communities, which is more important than the motivation of maximising profits for some individuals or firms. Thirdly, China, as the last major player on the planet to take on economic growth, comes from traditions fundamentally differ- ent from those economies that have experienced the Industrial Revolution previously. Its challenges with sustainability and en- vironmental conservation predate Western economics by millennia, and it is implementing policies domestically and starting to work on the world stage that acknowledges that the surroundings are the host for any economic and socio-political system. 相似文献
1000.
The important role that non-motorised transport plays in urban sustainability is discussed with particular reference to the developing world and to the links between environmental and poverty issues. The significance of non-motorised transport in terms of reduced pollution, income maintenance for the poor and providing transport for vulnerable groups is stressed and placed within the general context of sustainable development. More specifically, evidence is presented for rickshaws in Calcutta, which demonstrates the vital role that non-motorised transport must play if sustainable development objectives are to be met. This evidence indicates that if rickshaws were to disappear from Calcutta's streets there would not only be significant increases in air pollution but also a substantial increase in the numbers of people living in poverty. 相似文献