Rapid prenatal diagnosis of Patau's syndrome in a fetus with an abdominal wall defect by 72 hour culture of cells from amniotic fluid

A woman in the 32nd week of pregnancy was referred for investigation because of fetal abnormalities, including an abdominal wall defect, detected by ultrasonography. In view of the increased risk of chromosome abnormality, amniocentesis was performed to enable informed decisions about the management of the pregnancy and delivery to be taken. Cells from the liquor were inoculated into standard lymphocyte culture medium and incubated for 72 h. Slides with a high mitotic index and good quality metaphases, comparable to those from a blood culture, were obtained after harvesting. Cytogenetic analysis showed the karyotype to be 46,XY,—14,+t(13ql4q), which is consistent with Patau's syndrome. This technique appears to be an option for rapid karyotyping in cases of abdominal wall defect, where a chromosomal abnormality is suspected.     

Native fluorescence of unstained cryo-sections of the cervix uteri compared with histological observations

The excellent technical assistance of H. Führer and I. Leib is appreciated. Part of the work was supported by the Bundesministerium für Forschung und Technologie, the Deutsche Forschungsgemeinschaft, the Fonds der Chemischen Industrie, and from the Leitz and Hoechst companies. This paper is part of the M. D. thesis of Ch.L.     

The Oklo phenomenon

Until recently, scientists believed that the chemical elements were synthesized only in stars. The discovery of the Oklo phenomenon in the Republic of Gabon in 1972 has revealed, however, that a nuclear “fire” had existed on the earth and largescale transmutations of the elements were occurring on our planet 1.7·10⁹ years ago. The formation of natural (or Pre-Fermi) reactors is closely related to the appearance of life on our planet earth. The Pre-Fermi reactors were probably never formed until about 2·10⁹ years ago, when oxygen was injected into the earth's atmosphere by a new generation of living organisms carrying out photosynthesis.