Ecotoxicological testing with new kinetic Photorhabdus luminescens growth and luminescence inhibition assays in microtitration scale

Miniaturized luminescence and growth inhibition assays in microtitration plates with the terrestric enthomopathogenic nematode symbiont Photorhabdus luminescens (DSMZ 3368T) are presented and compared with standardized tests with Vibrio fischeri (DSMZ 7151/NRRLB-11177) and Pseudomonas putida (DSMZ 50026). Toxicological parameters (EC and G values) of selected reference toxicants (e.g. heavy metals and environmental samples) were obtained at different temperatures and without sodium chloride supplementation. Kinetic data recordings were compared with results of a cuvette test protocol using integral and endpoint calculation. Growth inhibition experiments with reference samples reveal similar or higher sensitivities as for the Vibrio or Pseudomonas spp. The luminescence inhibition assay shows reduced sensitivities to most of the reference samples compared with the V. fischeri standard assay. But G values obtained with other standardized aquatic assay systems with daphnids, algae and growth inhibition assays with V. fischeri and Ps. putida correspond more closely to data observed with Ph. luminescens. The test procedures are easily to perform and to evaluate and seem to be reliable alternatives to the established protocols at low osmolarities. The sample specifity of the toxic responses of the marine and the terrestric strain recommends to employ both assays to determine the toxic potential of environmental samples. This will support to reduce false positive results in future investigations.     

Inverse modeling of BTEX dissolution and biodegradation at the Bemidji, MN crude-oil spill site

The U.S. Geological Survey (USGS) solute transport and biodegradation code BIOMOC was used in conjunction with the USGS universal inverse modeling code UCODE to quantify field-scale hydrocarbon dissolution and biodegradation at the USGS Toxic Substances Hydrology Program crude-oil spill research site located near Bemidji, MN. This inverse modeling effort used the extensive historical data compiled at the Bemidji site from 1986 to 1997 and incorporated a multicomponent transport and biodegradation model. Inverse modeling was successful when coupled transport and degradation processes were incorporated into the model and a single dissolution rate coefficient was used for all BTEX components. Assuming a stationary oil body, we simulated benzene, toluene, ethylbenzene, m,p-xylene, and o-xylene (BTEX) concentrations in the oil and ground water, respectively, as well as dissolved oxygen. Dissolution from the oil phase and aerobic and anaerobic degradation processes were represented. The parameters estimated were the recharge rate, hydraulic conductivity, dissolution rate coefficient, individual first-order BTEX anaerobic degradation rates, and transverse dispersivity. Results were similar for simulations obtained using several alternative conceptual models of the hydrologic system and biodegradation processes. The dissolved BTEX concentration data were not sufficient to discriminate between these conceptual models. The calibrated simulations reproduced the general large-scale evolution of the plume, but did not reproduce the observed small-scale spatial and temporal variability in concentrations. The estimated anaerobic biodegradation rates for toluene and o-xylene were greater than the dissolution rate coefficient. However, the estimated anaerobic biodegradation rates for benzene, ethylbenzene, and m,p-xylene were less than the dissolution rate coefficient. The calibrated model was used to determine the BTEX mass balance in the oil body and groundwater plume. Dissolution from the oil body was greatest for compounds with large effective solubilities (benzene) and with large degradation rates (toluene and o-xylene). Anaerobic degradation removed 77% of the BTEX that dissolved into the water phase and aerobic degradation removed 17%. Although goodness-of-fit measures for the alternative conceptual models were not significantly different, predictions made with the models were quite variable.     

以科技进步为先导 以质量求生存

一九九○年五月四日,在建设部老干部活动室召开了环保新产品《增强聚丙烯压滤机》新闻发布会。新闻发布会由中国环保工业协会办公室主任赵连春主持。参加会议的有国家环保局、机械电子部、解放军环办、石化部、建设部、冶金部等有关领导,参加会议的还有中央电视台、中国环境报、经济日报、建设报、市场信息、《中国环境科学》、《环境科学动态》、《环境工程》、《石化》、《冶金》等编辑部。会上首先由新闻发布人-中国环保工业协会副秘书长邵本德同志讲话。接着由浙江省余杭县办公室主任汪士云、杭州良渚压滤机厂厂长朱兴源同志介绍工厂情况及环保新     

Integrated Measures of Anthropogenic Stress in the U.S. Great Lakes Basin

Integrated, quantitative expressions of anthropogenic stress over large geographic regions can be valuable tools in environmental research and management. Despite the fundamental appeal of a regional approach, development of regional stress measures remains one of the most important current challenges in environmental science. Using publicly available, pre-existing spatial datasets, we developed a geographic information system database of 86 variables related to five classes of anthropogenic stress in the U.S. Great Lakes basin: agriculture, atmospheric deposition, human population, land cover, and point source pollution. The original variables were quantified by a variety of data types over a broad range of spatial and classification resolutions. We summarized the original data for 762 watershed-based units that comprise the U.S. portion of the basin and then used principal components analysis to develop overall stress measures within each stress category. We developed a cumulative stress index by combining the first principal component from each of the five stress categories. Maps of the stress measures illustrate strong spatial patterns across the basin, with the greatest amount of stress occurring on the western shore of Lake Michigan, southwest Lake Erie, and southeastern Lake Ontario. We found strong relationships between the stress measures and characteristics of bird communities, fish communities, and water chemistry measurements from the coastal region. The stress measures are taken to represent the major threats to coastal ecosystems in the U.S. Great Lakes. Such regional-scale efforts are critical for understanding relationships between human disturbance and ecosystem response, and can be used to guide environmental decision-making at both regional and local scales.     

Motor vehicle restraint system use and risk of spine injury

OBJECTIVE: Motor vehicle collision (MVC)-related spinal injury is a severe and often permanently disabling injury. In addition, strain injuries have been reported as a common outcome of MVCs. Although advances in automobile crashworthiness have reduced both fatalities and severe injuries, the impact of varying occupant restraint systems (seatbelts and airbags) on thoracolumbar spine injuries is unknown. This study examined the relationship between the occurrence of mild to severe cervical and thoracolumbar spine injury and occupant restraint systems among front seat occupants involved in frontal MVCs. METHODS: A retrospective cohort study was conducted among subjects obtained from the 1995-2004 National Automotive Sampling System. Cases were identified based on having sustained a spine injury of >/=1 on the Abbreviated Injury Scale (AIS), 1990 Revision. Risk risks (RRs) and 95% confidence intervals (CIs) were computed comparing occupant restraint systems with unrestrained occupants. RESULTS: We found an overall incidence of AIS1 cervical (11.8%) and thoracolumbar (3.7%) spinal injury. Seatbelt only restraints were associated with increased cervical AIS1 injury (RR = 1.40, 95% CI 1.04-1.88). However, seatbelt only restraints showed the greatest risk reduction for AIS2 spinal injuries. Airbag only restraints reduced thoracolumbar AIS1 injuries (RR = 0.29, 95% CI 0.08-1.04). Seatbelt combined with airbag use was protective for cervical AIS3+ injury overall (RR = 0.29, 95% CI 0.14-0.58), cervical neurological injury (RR = 0.19, 95% CI 0.05-0.81), and thoracolumbar AIS3+ injury overall (RR = 0.20, 95% CI 0.05-0.70). CONCLUSIONS: The results of this study suggest that seatbelts alone or in combination with an airbag increased the incidence of AIS1 spinal injuries, but provide protection against more severe injury to all regions of the spine. Airbag deployment without seatbelt use did not show increased protection relative to unrestrained occupants.     

Plants grow better if seeds see green

We report on the response of dry plant seeds to their irradiation with intense green light applied at biostimulatory doses. Red and near-infrared light delivered by lasers or arrays of light emitting diodes applied at such doses have been shown previously by us to have effects on mammalian cells. Effects include cell proliferation and elevation of cell vitality, and have practical applications in various biomedical fields. Growth processes induced by photoreceptor stimulation (phytochromes and cryptochromes) in plant seeds with green light were described so far only for imbibed seeds. In this paper, we show that irradiation of dry cress, radish and carrot seeds with intense green light (laser or arrays of light emitting diodes), applied at biostimulatory doses, resulted in a significant increase in biomass—14, 26, and 71 days after seeding, respectively. In the case of radish and carrot, the irradiation led to important changes in the root-to-foliage surface ratio. Seeds with a potential to grant growth acceleration could be of special interest in agricultural applications, and could even compensate for shorter growth seasons caused by climate change.     

Microscale analytical methods for the quantitative detection of PCBs and PAHs in small tissue masses

Microscale methods (MM) were evaluated and compared to traditional methods (TM) for measuring polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) in spiked and standard reference fish and mussel tissues. MMs are advantageous because they use small tissue masses (ca. 100 mg), and maintain sensitivity through reducing final extract volume (traditionally 1 ml) by an order of magnitude or more (40 μl—PCBs; 100 μl—PAHs). Procedural losses occurred in the MMs’ combined cleanup/primary evaporation step (19% PAHs; 6% PCBs), and the final extract concentration (14% PAHs; 22% PCBs). The PAH MM performed comparably to the TM. Although most PCBs had recoveries >50%, the PCB MM generally yielded lower recoveries than the TM. Average method detection limits were 0.6 μg/kg (TM) and 1.0 μg/kg (MM) for PCBs and 25.7 μg/kg (TM) and 27.7 μg/kg (MM) for PAHs. MMs described for PCB and PAH tissue samples are potentially viable alternatives to TMs, and could lead to cost savings in bioaccumulation/toxicity tests.     

Effluent profile of commercially used low-phosphorus fish feeds

Excess phosphorus (P) in aquaculture feeds contributes to the eutrophication of natural waters. While commercially available low-P (LP) fish feeds have been developed, there is uncertainty about their potential to reduce effluent P while maintaining fish growth relative to regular P (RP) feeds. We therefore simulated commercial aquaculture conditions and fed for 55 days rainbow trout (approximately 190 kg/raceway, n = 3 raceways/diet) RP (1.4% total P) and LP (1.0%) feeds then determined effluent P levels, fish growth, and feed costs. Excretions of fecal-P and soluble-P, but not particulate-P, in effluents were greater in RP than in LP ponds. Fish growth, bone-P and plasma-P were similar between diets, demonstrating that LP feeds can lower effluent P levels without compromising growth. Costs were 0.97 dollars/kg fish production for LP feeds, and 0.74 dollars/kg for RP. Because feed is the largest variable cost in commercial aquaculture, the use of LP feeds can significantly increase production costs.     

Predicting Gran alkalinity and calcium concentrations in river waters over a national scale using a novel modification to the G-BASH model

Monthly stream water calcium and Gran alkalinity concentration data from 11 sub-catchments of the Nether Beck in the English Lake District have been used to appraise the transferability of the Scottish, River Dee-based G-BASH model. Readily available riparian zone geochemistry and flow paths were used initially to predict minimum and mean stream water concentrations at the Nether Beck, based on calibration equations from the River Dee catchment data. Predicted values significantly exceeded observed values. Differences in runoff between the two areas, leading to a dilution effect in the Nether Beck, explained most of the difference between observed and predicted values. Greater acid deposition in the Lake District also reduced stream water Gran alkalinity concentrations in that area. If regional differences in precipitation, evapotranspiration and pollutant deposition are incorporated into the model, it may then be used reliably to predict catchment susceptibility to acidification over a wide regional (national) scale.     

Phosphorus removal in vegetated filter strips

Vegetated filter strips (VFS) are used recently for removal, at or near the source, of sediment and sediment-bound chemicals from cropland runoff. Vegetation within the flowpath increases water infiltration and decreases water turbulence, thus enhancing pollutant removal by sedimentation within filter media and infiltration through the filter surface. Field experiments have been conducted to examine the efficiency of vegetated filter strips for phosphorus removal from cropland runoff with 20 filters with varying length (2 to 15 m), slope (2.3 and 5%), and vegetated cover, including bare-soil plots as control. Artificial runoff used in this study had an average phosphorus concentration of 2.37 mg L(-1) and a sediment concentration of 2700 mg L(-1). The average phosphorus trapping efficiency of all vegetated filters was 61% and ranged from 31% in a 2-m filter to 89% in a 15-m filter. Filter length has been found to be the predominant factor affecting P trapping in VFS. The rate of inflow, type of vegetation, and density of vegetation coverage had secondary influences on P removal. Short filters (2 and 5 m), which are somewhat effective in sediment removal, are much less effective in P removal. Increasing the filter length beyond 15 m is ineffective in enhancing sediment removal but is expected to further enhance P removal. Sediment deposition, infiltration, and plant adsorption are the primary mechanisms for phosphorus trapping in VFS.