The Role of Women in Water Management and Conflict Resolution in Marsabit,Kenya

We employed qualitative methods to explore how conflict over water collection and use impacts women, and the role that women play in water management and conflict resolution in Marsabit, Kenya. Conflicts between domestic and livestock water led to insufficient water for domestic use and intra-household conflict. Women’s contributions to water management were valued, especially through informal initiatives, though involvement in statutory water management committees was not culturally appropriate. Promoting culturally appropriate ways to involve women in water management, rather than merely increasing the percentage of women on water committee, may reduce conflicts and increase women’s access to domestic water supplies.     

Human maternal uniparental disomy for chromosome 16 and fetal development

Two severely growth-retarded fetuses found to have maternal uniparental disomy (UPD) for chromosome 16 and trisomy 16 placental mosaicism both had an unfavourable outcome. Antenatally, the first case was complicated by an unexplained raised maternal serum alpha-fetoprotein concentration, preterm premature rupture of the membranes, and growth retardation detectable at 21 weeks' gestation, whilst the other had an unexplained raised maternal serum human chorionic gonadotrophin level, a two-vessel cord on ultrasound, and cessation of growth at 25 weeks. At post-mortem, both babies had an imperforate anus. Fetal maternal UPD may explain the poor outcome that occurs in some cases of confined placental mosaicism for chromosome 16 and is also associated with specific fetal abnormalities.     

Does the antidiabetic drug metformin affect embryo development and the health of brown trout (<Emphasis Type="Italic">Salmo trutta</Emphasis> f. <Emphasis Type="Italic">fario</Emphasis>)?

Background

Due to the rising number of type 2 diabetes patients, the antidiabetic drug, metformin is currently among those pharmaceuticals with the highest consumption rates worldwide. Via sewage-treatment plants, metformin enters surface waters where it is frequently detected in low concentrations (µg/L). Since possible adverse effects of this substance in aquatic organisms have been insufficiently explored to date, the aim of this study was to investigate the impact of metformin on health and development in brown trout (Salmo trutta f. fario) and its microbiome.

Results

Brown trout embryos were exposed to 0, 1, 10, 100 and 1000 µg/L metformin over a period from 48 days post fertilisation (dpf) until 8 weeks post-yolk sac consumption at 7 °C (156 dpf) and 11 °C (143 dpf). Chemical analyses in tissues of exposed fish showed the concentration-dependent presence of metformin in the larvae. Mortality, embryonic development, body length, liver tissue integrity, stress protein levels and swimming behaviour were not influenced. However, compared to the controls, the amount of hepatic glycogen was higher in larvae exposed to metformin, especially in fish exposed to the lowest metformin concentration of 1 µg/L, which is environmentally relevant. At higher metformin concentrations, the glycogen content in the liver showed a high variability, especially for larvae exposed to 1000 µg/L metformin. Furthermore, the body weight of fish exposed to 10 and 100 µg/L metformin at 7 °C and to 1 µg/L metformin at 11 °C was decreased compared with the respective controls. The results of the microbiome analyses indicated a shift in the bacteria distribution in fish exposed to 1 and 10 µg/L metformin at 7 °C and to 100 µg/L metformin at 11 °C, leading to an increase of Proteobacteria and a reduction of Firmicutes and Actinobacteria.

Conclusions

Overall, weight reduction and the increased glycogen content belong to the described pharmaceutical effects of the drug in humans, but this study showed that they also occur in brown trout larvae. The impact of a shift in the intestinal microbiome caused by metformin on the immune system and vitality of the host organism should be the subject of further research before assessing the environmental relevance of the pharmaceutical.

     

Water Supply Reliability Tradeoffs between Removing Reservoir Storage and Improving Water Conveyance in California

Population growth, climate change, aging infrastructure, and changing societal values alter how water must be managed in the 21st Century. O'Shaughnessy Dam, located in Yosemite National Park, has been identified as a possible candidate for dam removal. It is a component of San Francisco's Hetch Hetchy System and is operated for water supply and hydropower. This article describes a spatially scaled approach to analyze water reliability without O'Shaughnessy Dam, but with improved water conveyance between the Hetch Hetchy System and existing reservoirs and aqueducts at the watershed, regional Bay Area, and statewide scales. It broadens previous research to highlight larger scale implications of removing O'Shaughnessy Dam and evaluates the role of improved water conveyance for water management. CALifornia Value Integrated Network, a large‐scale hydro‐economic model evaluates intertied water management using estimated urban and agricultural water demands for year 2050 with 72‐year historical and warm, dry hydrologic conditions. Results suggest that O'Shaughnessy Dam can be removed with additional conveyance at any spatial scale while maintaining water reliability. With a warm, dry climate, water reliability, and storage decline, indicating removing O'Shaughnessy Dam may have less effect on water management than climate change when conveyance is improved between the Hetch Hetchy System and nearby systems. Improving water conveyance can sometimes substitute for water storage in storage‐rich watersheds.     

Management of the Spring Snowmelt Recession in Regulated Systems

In an effort to restore predictable ecologically relevant spring snowmelt recession flow patterns in rivers regulated by dams, this study defined a methodology by which spring flow regimes can be modeled in regulated systems from the quantifiable characteristics of spring snowmelt recessions in unregulated rivers. An analysis of eight unregulated rivers across the Sierra Nevada mountain range in California found that unregulated systems behaved similarly with respect to seasonal spring patterns and recession limb curvature, and thus prescribed flows could be designed in a manner that mimics those predictable characteristics. Using the methodology to quantify spring recession flows in terms of a daily percent decrease in flow, a series of flow recession scenarios were created for application in an existing hydrodynamic model for the regulated Rubicon River. The modeling results showed that flow recessions with slow ramping rates similar to those observed in unregulated rivers (less than 10% per day) were likely to be protective of native aquatic species, such as the Foothill yellow‐legged frog, while flows that receded at greater rates would likely result in desiccation of egg masses and potential stranding of tadpoles and fry. Furthermore, recession rates of less than 10% per day provided the most spatially diverse hydraulic habitat in the modeled domain for an appropriate duration in spring to support all native species guilds and maximize aquatic biodiversity.     

Matching and mismatching stable isotope (δ13C and δ15N) ratios in fin and muscle tissue among fish species: a critical review

Using non-lethal tissue sampling for stable isotope analysis has become standard in many fields, but not for fishes, despite being desirable when species are rare or protected, when repeated sampling of individuals is required or where removal may bias other analyses. Here, we examine the utility of fish dorsal fin membrane as an alternative to muscle for analyzing δ¹³C and δ¹⁵N ratios in two reef fish species (blue cod Parapercis colias and spotty Notolabrus celidotus) that have differing feeding modes. Both species exhibited evidence of size-based feeding from fin δ¹⁵N values, but not from muscle. Blue cod fin δ¹⁵N increased steadily throughout the sampled size range (213–412 mm fork length), whereas spotty exhibited a distinct ontogenetic diet shift at approximately 120–140 mm fork length after which size-based feeding did not occur. Fin membrane was higher than muscle in δ¹³C in both species and in δ¹⁵N for blue cod, but fin δ¹⁵N was lower than muscle in spotty. The δ¹³C and δ¹⁵N fin–muscle offsets were constant in spotty regardless of size, while in blue cod, δ¹³C was constant with fish size, but δ¹⁵N offsets increased with increasing fish size. Non-lethal sampling utilizing fin tissue can be employed to estimate stable isotope values of muscle in fishes, but it is necessary to assess relationships among tissues and the effects of fish size on isotope values a priori for each species studied. Our data indicated that fin membrane may be a more sensitive tissue than muscle for detecting size-based feeding in some fish species using stable isotopes. A critical literature review revealed inconsistencies in tissue types tested, little understanding of tissue-specific trophic shift or turnover rates, and pseudo-replicated analyses leading to erroneous postulating of 1:1 relationships between tissues.     

Identifying trophic variation in a marine suspension feeder: DNA- and stable isotope-based dietary analysis in Mytilus spp.

Accurate field data on trophic interactions for suspension feeders are lacking, and new approaches to dietary analysis are necessary. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was integrated with stable isotope analysis to examine dietary patterns in suspension-feeding Mytilus spp. from seven spatially discrete locations within a semi-enclosed marine bay (Strangford Lough, Northern Ireland) during June 2009. Results of the two methods were highly correlated, reflecting dietary variation in a similar manner. Variation in PCR-DGGE data was more strongly correlated with the principal environmental gradient (distance from the opening to the Irish Sea), while values of δ ¹³C and δ ¹⁵N became progressively enriched, suggesting a greater dependence on animal tissue and benthic microalgae. Diatoms and crustaceans were the most frequently observed phylotypes identified by sequencing, but specific DNA results provided little support for the trophic trends observed in the stable isotope data. This combined approach offers an increased level of trophic insight for suspension feeders and could be applied to other organisms.     

Spatial Patterns of Soil Moisture as Affected by Shrubs, in Different Climatic Conditions

At three study sites, representing Mediterranean, semi-aridand mildly-arid climatic conditions, the effect of shrubs onthe spatial patterns of soil moisture was studied. At eachsite soil moisture was measured, on hillslopes, at thevicinity of 8 shrubs. For each shrub the measurements havebeen taken at 3 microenvironments, i.e. under the shrub (US),at the margins of shrub (MS) and between shrubs (BS). At themicroenvironments US and MS the measurements were taken at 3 directions: upslope, downslope and sideslope of the shrubs. At all sampling points soil samples were taken from 3 depths: 0–2, 2–5 and 5–10 cm. In addition, rock fragments cover percentage near the shrubs was determined. A soil moisture pattern was found, around each shrub,which is composed of a radial gradient and a downslope gradient. The radial gradient is expressed by soil moisture decreasingfrom the US microenvironment, in all directions, through the MS towards the BS microenvironment. The US microenvironmenthas a `spatial advantage' of higher soil moisture content dueto (1) relatively higher infiltration rate, (2) capture overlandflow from the BS area upslope that shrub and (3) low evaporationrate because of the shading effect.The downslope gradient is expressed by decreasing soil moisturefrom the upslope direction of each shrub (MS and US microenvironments) towards the downslope direction of that shrub (MS and US microenvironments, respectively). Thisgradient is controlled by the relatively high content of rockfragments near the shrubs at their upslope direction. Suchrock fragments spatial distribution is attributed to (1) thedetachment and transport of rock fragments by sheep and goatstrampling and (2) the effect of shrub on the continuity ofoverland flow and sediment transport. The effect of rockfragments is similar to that of shrubs regarding increasinginfiltration and decreasing evaporation rate. The relativelyhigh soil moisture at the upslope direction of each shrubenhances annuals growth producing a positive feedback loop:soil moisture – annuals growth – trampling. This sequencemaintains the typical rock fragments spatial organization andcontributes to the sustainability of the grazing system.At all the study sites at the US microenvironment there isa trend of decreasing soil moisture with increasing soildepth. At microenvironments MS and BS soil moisture increaseswith soil depth.The results are of great relevance for rehabilitationstrategies as they suggest that in order to combatdesertification in degraded semi-arid and mildly-arid areas,where the main land use is grazing, both shrubs and rockfragment should be kept at their present spatial distribution.     

H NMR metabolomics of earthworm exposure to sub-lethal concentrations of phenanthrene in soil

¹H NMR metabolomics was used to monitor earthworm responses to sub-lethal (50-1500 mg/kg) phenanthrene exposure in soil. Total phenanthrene was analyzed via soxhlet extraction, bioavailable phenanthrene was estimated by hydroxypropyl-β-cyclodextrin (HPCD) and 1-butanol extractions and sorption to soil was assessed by batch equilibration. Bioavailable phenanthrene (HPCD-extracted) comprised ∼65-97% of total phenanthrene added to the soil. Principal component analysis (PCA) showed differences in responses between exposed earthworms and controls after 48 h exposure. The metabolites that varied with exposure included amino acids (isoleucine, alanine and glutamine) and maltose. PLS models indicated that earthworm response is positively correlated to both total phenanthrene concentration and bioavailable (HPCD-extracted) phenanthrene in a freshly spiked, unaged soil. These results show that metabolomics is a powerful, direct technique that may be used to monitor contaminant bioavailability and toxicity of sub-lethal concentrations of contaminants in the environment. These initial findings warrant further metabolomic studies with aged contaminated soils.