Background and temperature effects on Uca panacea color change

Organismal coloration is used for communication, camouflage, and thermoregulation. These functions of body coloration may impose conflicting demands upon color-changing organisms. Here, we examined interacting thermoregulatory and camouflage color change responses when fiddler crabs Uca panacea were subject to simultaneously changing temperatures (10, 25, 40 °C) and backgrounds (black, white). Crab coloration lightened on a white background and at high temperatures and darkened on a black background and at low temperatures, reflecting the camouflage and thermoregulatory functions of color change. Synergistic background and temperature treatments (i.e., hot/white or cold/black) induced strong color change responses. When temperature and background were in conflict (i.e., hot/black or cold/white), responses to background coloration constrained thermoregulatory color change such that carapace coloration did not change. Such conflicts are likely to be common in nature, especially in highly heterogeneous environments. Throughout the experiment, males remained lighter than females and showed a greater response to the hot/white treatment, driven by a stronger response to high temperatures. These differences may reflect the physiological, morphological, and behavioral differences associated with sexual selection and sexual dimorphism in this species.     

Renal lesions in Greenland sledge dogs ( Canis familiaris ) exposed to a natural dietary cocktail of persistent organic pollutants

Persistent organic pollutants such as Polychlorinated biphenyls (PCB) and dichloro diphenyl trichloroethane (DDT) are known to exert various adverse health effects in wildlife mammals. The impact from dietary intake of minke whale (Balaenoptera acutorostrata) blubber high in organohalogen and other chemical contaminants on renal morphology and function was investigated via a controlled study on West Greenland sledge dogs (Canis familiaris). Our results showed significantly higher frequencies of glomerular, tubular, and interstitial lesions in the exposed group. Furthermore, higher urine protein?:?creatinine ratio and plasma urea levels were found in the exposed group, which indicated a negative impact on kidney function via tubular and glomerular dysfunctions. The lesions were similar to those observed in top predator marine species–such as seals and polar bears–and humans exposed via diet to persistent environmental pollutants. The lesions may have impacts on the overall health of these animals. It is reasonable to suggest that chronic exposure to organic pollutants via a country diet of high trophic level marine organisms may have an impact on renal morphology and function in Arctic apex predators in general as well as Inuit people.     

Histology of Sculpin spp. in east Greenland. I. Histological measures

There is inadequate morphological nomenclature and definition of organ pathology when using wild fish in biomonitoring of environmental pollution. The aim of this investigation was to provide a guide that assesses histology of Shorthorn (Myoxocephalus scorpius) and Fourhorn (Myoxocephalus quadricornis) sculpins obtained from a study using these two fish species as bioindicator organisms to evaluate environmental impact attributed to a dumpsite located in East Greenland. Specific histopathological lesions were quantified on the basis of Bernet et al. and all lesions were photomicrographed, evaluated, and placed into specific categories of five reaction patterns. These were circulatory disturbances, regressive changes, progressive alterations, inflammation, and tumors based on anatomical location, type, and severity. This method is postulated to enable an objective assessment of the histological integrity of fish gills and liver; thus, making it possible to compare sculpin pathology and reaction patterns at different locations. Data suggest that this updated histological guide might be used for identification and quantification of histological lesions when applying sculpins in biomonitoring programs in Greenland and other Arctic regions.     

Corridors promote fire via connectivity and edge effects

Landscape corridors, strips of habitat that connect otherwise isolated habitat patches, are commonly employed during management of fragmented landscapes. To date, most reported effects of corridors have been positive; however, there are long-standing concerns that corridors may have unintended consequences. Here, we address concerns over whether corridors promote propagation of disturbances such as fire. We collected data during prescribed fires in the world's largest and best replicated corridor experiment (Savannah River Site, South Carolina, USA), six -50-ha landscapes of open (shrubby/herbaceous) habitat within a pine plantation matrix, to test several mechanisms for how corridors might influence fire. Corridors altered patterns of fire temperature through a direct connectivity effect and an indirect edge effect. The connectivity effect was independent of fuel levels and was consistent with a hypothesized wind-driven "bellows effect." Edges, a consequence of corridor implementation, elevated leaf litter (fuel) input from matrix pine trees, which in turn increased fire temperatures. We found no evidence for corridors or edges impacting patterns of fire spread: plots across all landscape positions burned with similar probability. Impacts of edges and connectivity on fire temperature led to changes in vegetation: hotter-burning plots supported higher bunch grass cover during the field season after burning, suggesting implications for woody/herbaceous species coexistence. To our knowledge, this represents the first experimental evidence that corridors can modify landscape-scale patterns of fire intensity. Corridor impacts on fire should be carefully considered during landscape management, both in the context of how corridors connect or break distributions of fuels and the desired role of fire as a disturbance, which may range from a management tool to an agent to be suppressed. In our focal ecosystem, longleaf pine woodland, corridors might provide a previously unrecognized benefit during prescribed burning activities, by promoting fire intensity, which may assist in promoting plant biodiversity.     

Air infiltration measurements in a home using a convenient perfluorocarbon tracer technique

Using miniature perfluorocarbon tracer (PFT) sources and miniature passive samplers, both about the size of a cigarette, tests conducted in the laboratory and in a typical home successfully demonstrated the utility of the PFT kit as a means for implementing wide-scale infiltration measurements in homes. The PFT diffusion plug source, an elastomer containing a dissolved perfluorocarbon compound, was shown to emit vapors at the rate of about 0.1 to 5 nL/min, providing steady-state concentrations in a home of about 1 to 10 pL/L, i.e., parts per trillion by volume, when one source is deployed for each 300–500 ft² (28–46 m²) of living space. The emission rate from the diffusion source was predictable, but its dependence on both temperature and time suggested the development of alternative approaches. One such alternative, a liquid PFT permeation source, had emission rates which could be tailored over the range 10–20 nL/min, were independent of age for as long as the liquid remained ( 5 yr), and had significantly lower temperature dependence. A number of passive adsorption tube samplers performed reproducibly and identically (to within ±2%–3%) in laboratory tests. Together with a programmable tracer sampler, the miniature diffusion sources and samplers were deployed in a typical home; six PFT sources were uniformly deployed, three on each level of a two-story house. Multiple location sampling, as well as sampling in rooms with and without a miniature source, demonstrated that even in a house without forced-air circulation, a well-mixed modeling approach is justified. Analyses of the tracer samplers were performed back in the laboratory with an automatic electron-capture gas-chromatography system. The effects of the inside/outside temperature differential, as well as that of an open fireplace compared with a wood-burning stove, on the measured air infiltration rates were clearly demonstrated. Comparisons of the PFT tracer method with that of the SF₆ tracer decay approach showed the results of the two methods to be identical within experimental precision. With this miniature source and sampling tracer kit, infiltration rates in the range 0.2–5 air changes per hour can be measured over time-averaged periods of as little as 1 day up to several years.     

Atmospheric deposition of pesticides to an agricultural watershed of the Chesapeake Bay

The Choptank River watershed, located on the Delmarva Peninsula of the Chesapeake Bay, is dominated by agricultural land use, which makes it vulnerable to runoff and atmospheric deposition of pesticides. Agricultural and wildlife areas are in close proximity and off-site losses of pesticides may contribute to toxic effects on sensitive species of plants and animals. High-volume air samples (n = 31) and event-based rain samples (n = 71) were collected from a single location in the watershed representing regional background conditions. Surface water samples were collected from eight stations in the tidal portion of the river on five occasions during 2000. Chlorothalonil, metolachlor, atrazine, simazine, endosulfan, and chlorpyrifos were frequently detected in the air and rain, with maximal concentrations during the period when local or regional crops were planted. The wet deposition load to the watershed was estimated at 150 +/- 16, 61 +/- 7, and 51 +/- 6 kg yr(-1) for chlorothalonil, metolachlor, and atrazine, respectively. The high wet deposition load compared with the estimated annual usage for chlorothalonil (13%) and endosulfan (14-90%) suggests an atmospheric source from outside the watershed. Net air-water gas exchange fluxes for metolachlor varied from -44 +/- 19 to 9.3 +/- 4.1 ng m(-2) d(-1) with negative values indicating net deposition. Wet deposition accounted for 3 to 20% of the total metolachlor mass in the Choptank River and was a more important source to the river than gas exchange. Estimates of herbicide flux presented here are probably a low estimate and actual rates may be significantly higher in areas closer to pesticide application.     

Effects of different management strategies on long-term trends of Australian threatened and near-threatened mammals

Monitoring is critical to assess management effectiveness, but broadscale systematic assessments of monitoring to evaluate and improve recovery efforts are lacking. We compiled 1808 time series from 71 threatened and near-threatened terrestrial and volant mammal species and subspecies in Australia (48% of all threatened mammal taxa) to compare relative trends of populations subject to different management strategies. We adapted the Living Planet Index to develop the Threatened Species Index for Australian Mammals and track aggregate trends for all sampled threatened mammal populations and for small (<35 g), medium (35–5500 g), and large mammals (>5500 g) from 2000 to 2017. Unmanaged populations (42 taxa) declined by 63% on average; unmanaged small mammals exhibited the greatest declines (96%). Populations of 17 taxa in havens (islands and fenced areas that excluded or eliminated introduced red foxes [Vulpes vulpes] and domestic cats [Felis catus]) increased by 680%. Outside havens, populations undergoing sustained predator baiting initially declined by 75% but subsequently increased to 47% of their abundance in 2000. At sites where predators were not excluded or baited but other actions (e.g., fire management, introduced herbivore control) occurred, populations of small and medium mammals declined faster, but large mammals declined more slowly, than unmanaged populations. Only 13% of taxa had data for both unmanaged and managed populations; index comparisons for this subset showed that taxa with populations increasing inside havens declined outside havens but taxa with populations subject to predator baiting outside havens declined more slowly than populations with no management and then increased, whereas unmanaged populations continued to decline. More comprehensive and improved monitoring (particularly encompassing poorly represented management actions and taxonomic groups like bats and small mammals) is required to understand whether and where management has worked. Improved implementation of management for threats other than predation is critical to recover Australia's threatened mammals.