一株花生根际促生菌的筛选鉴定及其特性研究

从种植于红壤的健康花生根际,筛选出7株产吲哚乙酸(IAA)菌株,以菌株L4合成IAA的能力最强,培养24 h时IAA产生量达135.67μg.mL-1,且菌株L4具有解磷能力。通过菌株形态、生理生化特征测定及16SrRNA的保守序列鉴定,初步确定菌株L4为氯酚节杆菌(Arthrobacter chlorophenolicus),其GenBank登录号为JQ277449。菌株生长和发酵条件试验结果表明,菌株L4生长和分泌IAA的最佳培养条件并不完全一致,既能促进菌株生长又能合成较多IAA的最佳培养条件是初始pH值为5～6,装液量为50 mL.(250 mL)-1,30℃摇床培养24 h;促进菌株生长的最佳碳、氮源分别是麦芽糖和酵母粉,而提高IAA产生量的最佳碳源是木糖,最佳氮源是KNO3。     

Experimental study on the mechanism effect of seepage on microstructure of tailings

The deformation of microstructure of tailings caused by seepage is the main factor that leads the tailings dam to be damaged and lose stability. The effect of seepage on microstructure of tailings has been studied by a self-made observation testing apparatus of micro-mechanics and deformation of tailings, and its mechanism also has been explained in mechanics of particulate matter. The results show that the deformation response to microstructure of tailings is fast under load. The settlement of sample increases stepwise. Firstly, the fine particles crumble, then skeleton particles move, so as to noticeable settlement. Because the loose particles migrate taken by seepage water, the upper pores become bigger and lower pores become smaller. The relation of seepage water and settlement changing with time could be divided into two stages. In each stage, the rates of seepage and settlement reduce with time, and between the two stages, the rate of settlement increases significantly and the rate of seepage reduces with time. The seepage has significant effect on the particle size distribution of tailings in different depths, which has a little effect on the coarse tailings; the proportion of fine tailings increases significantly with the depth increasing; the minimum particle size decreases gradually and median diameter reduces stepwise with the depth increasing.     

硫化亚铁氧化自燃倾向性的实验研究

含硫油品储罐腐蚀产物FeS的氧化自燃是引起储罐火灾爆炸事故的主要原因。用同步热分析仪(STA)在30～900℃范围内对FeS进行热重热流分析,从物理吸附和化学反应的角度分析了不同粒径和不同升温速率FeS的自然氧化倾向性,计算了不同升温速率FeS的活化能。结果表明,FeS样品粒径越小,越容易发生氧化自燃反应;升温速率越大,FeS越不容易氧化。同时,不同升温速率条件下的FeS反应机制各不相同。从实验得出的动力学参数看出,FeS的氧化反应较复杂,而非简单的放热反应。     

影响文丘里除尘器性能的因素分析研究

分析研究了影响文丘里除尘器性能的因素,对多种影响因素与除尘效率、阻力及漏风之间的关系进行了回归分析,并推出了文丘里除尘器烟气带水的计算公式,为文丘里除尘器的设计、维护及安全经济运行提供了依据。     

黄河三角洲滨海湿地的维管束植物区系特征

在2007-2008年多次实地调查并参照相关资料基础上,对黄河三角洲滨海湿地进行了植被类型划分和湿地维管束植物生态类群、生活型划分,利用统计方法分析了研究区湿地维管束植物区系的科、属构成,地理分布区成分构成.研究表明,黄河三角洲滨海湿地有71科、199属、298种维管束植物,蓼科、藜科、苋科、豆科、菊科、禾本科、莎草科等7个大科为区系主体,上述7科共76属128种,占总属数的39.38%和总种数的43.99%;区系中单种属多,属的分化程度较高.区系中有59种盐生植物、46种水生植物、44种湿生植物、144种中生植物、7种旱生植物,水生植物、湿生植物的生活型以地面芽植物、地下芽植物为主,陆地中生植物、盐生植物的生活型以一年生植物为主.从分布区类型构成来看,区系中蕨类植物区域分布成分较多,种子植物中温带分布属最多,这反映出湿地植被具有一定的地带性特征,种子植物中热带分布属、世界分布属较多分别表明海洋性气候对湿地植被特征的影响及湿地植被的隐域性特征.     

电解液循环对废手机线路板碱性矿浆电解回收的影响

针对废手机线路板碱性矿浆电解资源化过程中电解液带来的污染问题,采用氨-氯化铵碱性矿浆电解液循环回收废手机线路板中金属,以降低能耗、减少污染.研究了氨-氯化铵碱性矿浆电解体系从废手机板制备阴极铜的过程中,电解液循环对Cu的回收率、电流效率、Cu纯度以及Cu和Ni、Zn、Pb等金属迁移转化的影响.经过7次电解液循环实验,结...     

中国秸杆焚烧及民用燃煤棕色碳排放的初步研究

近年来的研究表明,在BC(黑碳)和OC(有机碳)之间,还存在着一种有弱吸光能力的OC,因大多显棕黄色而被称为BrC(brown carbon,棕色碳). 广泛存在的秸杆焚烧和冬季大量民用燃煤的使用,使国内BrC排放严重,但鲜见对其排放量的测算. 采用七波段黑碳仪(aethalometer)方法,对夏季小麦秸杆焚烧过程及冬季民用炉燃煤过程产生的烟气进行现场监测,根据BrC与BC的光谱关联性差别,分化出R_BrC/BC(总光学衰减中BrC和BC的相对贡献). 结果表明:麦秆焚烧和民用燃煤烟气的R_BrC/BC分别为1.754±0.278和0.183±0.142. 借助R_BrC/BC值,结合现有的BC排放清单(2000年),初步推算出中国民用燃煤和秸杆田间焚烧BrC的排放总量(以BC当量计,下同)为(270.6±101.6)Gg,接近同期BC排放量的一半;其中秸杆焚烧的BrC排放量为(175.4±27.8)Gg,约占二者总量的65%;民用燃煤的BrC排放量为(95.2±73.7)Gg,约占35%. 该研究结果可为更全面的BrC排放测算奠定基础,并为研究BrC的大气化学及辐射强迫提供依据.      

Analysis of the phenolic compounds in root exudates produced by a subalpine coniferous species as responses to experimental warming and nitrogen fertilisation

In terrestrial ecosystems, plant root exudates clearly play a crucial role in the belowground ecosystem. However, there have been few reports on root exudates from field-grown plants or mature trees in situ, especially when exposed to experimental warming. In this study, we adopted and modified a culture-based cuvette system developed especially for root exudation collection in the field to collect soluble root exudates of a subalpine coniferous species, Abies faxoniana, under experimental warming and nitrogen fertilisation treatments. We then analysed the chemical composition and relative abundance of root exudates using gas chromatography-mass spectrometry (GC-MS). The major chemical constituents of root exudates were phenols and their derivatives of all the different treatments, such as 2,6-di-tert-butyl-4-methylphenol. Experimental warming had significant effects on the relative contents of major compounds and an increase effect on the total phenolic acid compounds. By contrast, there were small significant effects of N fertilisation on root exudation and no significant effects of the warming×N fertilisation interaction. Meanwhile, warming also markedly increased soil polyphenol oxidase activity and it may be soil ecological adjustment response to changes of root exudation under global climate warming.     

Sex- and performance-based escape behaviour in an Asian agamid lizard,Phrynocephalus vlangalii

In lizards, males are predicted to sprint faster and run for longer than females by virtue of higher testosterone levels and differences in morphology. Consequently, escape behaviour is also predicted to be associated with sex and locomotor performance, yet these links have rarely been explored. Here, we tested whether escape behaviour is associated with locomotor performance in the toad-headed agama, Phrynocephalus vlangalii, and whether it is sex-dependent. This species is also characterized by elaborate tail displays, which we examined as a potential pursuit-deterrent signal. Tail waves were performed by a very small proportion (2/58, 3 %) of individuals during predatory trials, suggesting that tail signalling functions exclusively in a social context. To understand the relationships between sex, escape behaviour and performance, we first measured escape behaviour (flight initiation distance, flight distance—measured differently compared to previous studies of lizard escape behaviour, and refuge use) in the field before measuring maximal sprint speed and endurance on the same individuals in the laboratory. Flight initiation distance did not differ between the sexes and was unrelated to performance capacity (maximal endurance and sprint speed) but was positively related to body size with larger individuals fleeing earlier. Males fled farther than females, but flight distance was also unrelated to either endurance or sprint speed. Interestingly, faster females were less likely to enter a refuge than slower females, whereas sprint speed and the probability of taking refuge were unrelated for males. Our results suggest that when males and females are not obviously sexually dimorphic, they are more likely to overlap in escape tactics.     

Trophic mode and organics metabolic characteristic of fungal community in swine manure composting

Fungal trophic modes and substrates utilization ability was observed in composting. Fungi had the higher diversity and more trophic types in thermophilic phase. Fungi had the higher metabolic potential in fresh swine manure and mature production. Redox potential, organics and moisture are main factors impacting fungal community. Composting reduced pathogenic fungi and enrich dung saprotroph fungi in swine manure. The succession of fungal community, trophic mode and metabolic characteristics were evaluated in 60 days composting of swine manure by high-throughput sequencing, FUNGuild and Biolog method, respectively. The result showed that the fungal community diversity reached to the highest level (76 OTUs) in the thermophilic phase of composting, then sustained decline to 15 OTUs after incubation. There were 10 fungal function groups in the raw swine manure. Pathotroph-saprotroph fungi reached to 15.91% on Day-10 but disappeared on Day-60. Dung saprotroph-undefined saprotroph fungi grown from 0.19% to 52.39% during the treatment. The fungal community had more functional groups but the lower substrate degradation rates in the thermophilic phase. The fungal communities on Day-0 and Day-60 had the highest degradation rates of amino acids and polymers, respectively. Redundancy analysis showed that ORP (49.6%), VS/Ash (45.3%) and moisture (39.2%) were the main influence factors on the succession of fungal community in the swine manure composting process.