Simultaneous Concentration of Bovine Viruses and Agricultural Zoonotic Bacteria from Water Using Sodocalcic Glass Wool Filters

Infiltration and runoff from manured agricultural fields can result in livestock pathogens reaching groundwater and surface waters. Here, we measured the effectiveness of glass wool filters to simultaneously concentrate enteric viruses and bacteria of bovine origin from water. The recovery efficiencies were determined for bovine viral diarrhea virus types 1 and 2, bovine rotavirus group A, bovine coronavirus, poliovirus Sabin III, toxigenic Escherichia coli ,and Campylobacter jejuni seeded into water with three different turbidity levels (0.5, 215, and 447 NTU). Twenty liters of dechlorinated tap water (pH 7) were seeded with the test organisms, and then passed through a glass wool filter using a peristaltic pump (flow rate = 1 liter min^?1). Retained organisms were eluted from the filters by passing beef extract-glycine buffer (pH 9.5) in the direction opposite of sample flow. Recovered organisms were enumerated by qPCR except for C. jejuni, which was quantified by culture. Mean recovery efficiencies ranged from 55 to 33 % for the bacteria and 58 to 16 % for the viruses. Using bootstrapping techniques combined with Analysis of Variance, recovery efficiencies were found to differ among the pathogen types tested at the two lowest turbidity levels; however, for a given pathogen type turbidity did not affect recovery except for C. jejuni. Glass wool filtration is a cost-effective method for concentrating several waterborne pathogens of bovine origin simultaneously, although recovery may be low for some specific taxa such as bovine viral diarrhea virus 1.     

A Spatially Explicit Model for Mapping Headwater Streams

Headwater streams are the primary sources of water in a drainage network and serve as a critical hydrologic link between the surrounding landscape and larger, downstream surface waters. Many states, including North Carolina, regulate activity in and near headwater streams for the protection of water quality and aquatic resources. A fundamental tool for regulatory management is an accurate representation of streams on a map. Limited resources preclude field mapping every headwater stream and its origin across a large region. It is more practical to develop a model for headwater streams based on a sample of field data that can then be extrapolated to a larger area of interest. The North Carolina Division of Water Quality has developed a cost‐effective method for modeling and mapping the location, length, and flow classification (intermittent and perennial) of headwater streams. We used a multiple logistic regression approach that combined field data and terrain derivatives for watersheds located in the Triassic Basins ecoregion. Field data were collected using a standard methodology for identifying headwater streams and origins. Terrain derivatives were generated from digital elevation models interpolated from bare‐earth Light Detection and Range data. Model accuracies greater than 80% were achieved in classifying stream presence and absence, stream length and perennial stream length, but were not as consistent in predicting intermittent stream length.     

The energy and carbon intensity of wine distribution: A study of logistical options for delivering wine to consumers

Logistics within the food and beverage sector are often energy-intensive, especially for the wine industry. We consider how California wines may be routed to U.S. consumers near and far, basing scenarios and supporting data on interviews and literature review. We use a web-based tool, CargoScope, to calculate the energy and carbon emissions associated with each transportation link and storage echelon. We find that supply chain configurations can result in vastly different energy and emissions' profiles, varying by up to a factor of 80, and discuss how these results could be incorporated into a winery's overall sustainability strategy.     

斜坡区和隆起区断裂密集带差异性及其对油气分布的控制作用

此文以霸县凹陷文安斜坡和饶阳凹陷留楚构造为研究对象,对比了斜坡区和隆起区断裂密集带的特征及其对不同构造部位油气分布的控制作用。在三维地震资料、钻井资料和已发现油气藏特征的基础上,利用断裂密集带识别方法和分类方案,识别出文安斜坡和留楚构造的断裂密集带,并对其进行了分类。对比两种构造部位断裂密集带发育的差异性及对油气分布的控制作用,认为隆起区(留楚构造)相对于斜坡区(文安斜坡)断裂密集带发育数量较少,规模大,斜坡区断裂密集带主要以顺向背形断裂密集带为主,而隆起区主要以屋脊背形断裂密集带为主,隆起区油气主要分布在顺向背形断裂密集带内,斜坡区主要分布在屋脊背形断裂密集带内部及靠近生烃中心的边部。