Training future experts in “biodiversity and ecosystem services”: a progress report

Biodiversity is essential for multiple aspects of human life and well-being, but many current assessments of the functioning of biodiversity and ecosystems, understanding of risks posed by environmental change and the best practice of their management of ecosystems are lacking a unified scientific and conceptual basis. Methods such as scenario analysis, and terms such as ecosystem services, are widely used, but their meaning is understood in many different ways depending on context, user needs and experience of researchers. In order to advance the conceptual basis for ecosystem analysis and management in a rapidly changing world, as well as the ability of young scientists to reflect upon these concepts, we have organised five 2-week-long summer schools in Peyresq, a remote village in the Southern French Alps. In total 173 participants have worked intensively with 69 experienced researchers and a team of conveners and tutors in order to discuss a broad range of views on topics on ecosystem analysis and functioning. Topics ranged from conditions of and threats to various ecosystems due to environmental change, models and scenarios for assessment, stakeholder perceptions and needs for information, to the social and economic contexts for biodiversity. We report our experience from these schools, present the training concept which has emerged from them and suggest lines of further development.

Effects of a phycotoxin,okadaic acid,on oyster heart cell survival

Okadaic acid (OA) is a dinoflagellate toxin which accumulates in shellfish producing diarrhetic shellfish poisoning (DSP) in humans. It was found that OA is a highly selective inhibitor of protein phosphatase types 1 (PP1) and 2A (PP2A) which produces a marked increase in phosphorylation of several proteins, including p38 mitogen-activated protein (MAP) kinase. The cytotoxicity attributed to OA and the effects on p38 MAP kinase and calcium current were examined in the oyster Crassostrea gigas in this study. Data showed that p38 MAP kinase is strongly expressed in oyster heart and that OA bioaccumulated in cultured heart cells. Hence the effects of OA was tested in vitro and in vivo on oysters. OA was found to (i) exert a positive chronotropic effect on cultured atrial cardiomyocytes which is related to an increase in calcium current via PKC as shown by patch clamp measurements, (ii) produce an activation/phosphorylation of MAP kinase as shown by Westernblot while the non-phosphorylated p38 remained constant during treatment, (iii) did not induce a pro-apoptotic effect. Data suggest that OA may also stimulate the anti-apoptotic pathway by phosphatase inhibition.     

Sex in the beach: spermatophores, dermal insemination and 3D sperm ultrastructure of the aphallic mesopsammic Pontohedyle milaschewitchii (Acochlidia, Opisthobranchia, Gastropoda)

Sperm transfer via spermatophores is common among organisms living in mesopsammic environments, and is generally considered to be an evolutionary adaptation to reproductive constraints in this habitat. However, conclusions about adaptations and trends in insemination across all interstitial taxa cannot be certain as differences in mode of insemination via spermatophores do exist, details of insemination are lacking for many species, and evolutionary relationships in many cases are poorly known. Opisthobranch gastropods typically transfer sperm via reciprocal copulation, but many mesopsammic Acochlidia are aphallic and transfer sperm via spermatophores, supposedly combined with dermal fertilisation. The present study investigates structural and functional aspects of sperm transfer in the Mediterranean microhedylacean acochlid Pontohedyle milaschewitchii. We show that spermatophore attachment is imprecise. We describe the histology and ultrastructure of the two-layered spermatophore and discuss possible functions. Using DAPI staining of the (sperm-) nuclei, we document true dermal insemination in situ under the fluorescence microscope. Ultrastructural investigation and computer-based 3D reconstruction from TEM sections visualise the entire spermatozoon including the exceptionally elongate, screw-like keeled sperm nucleus. An acrosomal complex was not detected. From their special structure and behaviour we conclude that sperm penetrate epithelia, tissues and cells mechanically by drilling rather than lysis. Among opisthobranchs, dermal insemination is limited to mesopsammic acochlidian species. In this spatially limited environment, a rapid though imprecise and potentially harmful dermal insemination is discussed as a key evolutionary innovation that could have enabled the species diversification of microhedylacean acochlidians.     

Vergleich der Raumgliederung Europas mit bestehenden Raumgliederungen – Teil 3

Background, aim and scope The first two articles of this series (Hornsmann et al. 2008; Weustermann et al. 2009) comprised how the Ecological Land Classification of Europe (ELCE) was calculated. Moreover several ecological classes were described exemplarily. In this part 3 of the series the ELCE is compared with two European ecoregionalisations at several levels of differentiation. These are the Digital Map of European ecological regions (DMEER) and the Environmental Stratification of Europe (EnS). In addition the ELCE was opposed to the ecoregionalisation of Germany. The alignment was carried out both functionally and statistically and served as a basis for the examination of the functional consistency of the ELCE. Materials and methods The ecological land classifications were described concerning their input data, the method of derivation and the intended use. The ELCE and the selected three ecoregionalisations were intersected in a GIS and crosstabulated with regard to the acreage of each polygon. These cross tabulations were performed by listing the percentage of ecoregions of e.?g. DMEER to the ecological classes of the ELCE (EC) and vice versa. The similarity of the compared ecoregions was estimated by means of statistics. Results The comparisons show high rates of similarity of the ELCE to the European land classifications. The mean overlap of the EC with the classes of the DMEER is 84 and 69?% vice versa. The EC are covered by the strata of the EnS with 67 and 72?%, respectively. In Germany, ELCE reflects the most important ecological conditions. The mean overlap of the ecoregions in this case is 75 and 80?% vice versa. Discussion The classes of the DMEER show a better overlap of the EC as vice versa due to their larger spatial extent. The main difference between the ELCE and the EnS is based on the procedure of the derivation of the regionalisations. While the classes of the EnS are more differentiated in the South of Europe, the ecoregions of ELCE are smaller and more subdivided in the middle of Europe. The high overlap between the ELCE and the ecoregionalisation of Germany can be indicative for the good account of the ecological structures of the ELCE in the European dimension. Conclusions The ELCE is functionally plausible and a useful complement to the existing land classifications of Europe. Nevertheless, it cannot substitute land classifications on the national level calculated with more detailed input data. The ELCE can be used testing the representativity of environmental monitoring networks in Europe. Recommendations In future, the ELCE should be compared with other national landscape classifications. A new calculation of ELCE would only be useful after the development of a highly powerful derivation method and on the basis of more detailed input data. Moreover, due to the observed climate change in the past 20 years, up-to-date data on climate should be used for the calculations. Perspectives The ELCE could be proven to be functionally consistent. Therefore the fourth article will focus on the investigation of the landscape representativity of environmental monitoring networks on the basis of the ELCE.     

Twenty years of elemental analysis of marine biota within the German Environmental Specimen Bank—a thorough look at the data

Purpose  

As one component of the German ecological environment observation, the Environmental Specimen Bank program was initiated in the mid-1980s. Under the program, representative specimens of marine, fresh water, and terrestrial ecosystems are sampled regularly and archived under chemically stable conditions. An initial characterization of the samples provides data regarding the status quo of the respective ecosystems. The aim of the present publication is to give insight into these real-time monitoring data, which have been generated for the last 10 to 20 years. This is done exemplarily for the heavy metals cadmium (Cd), mercury (Hg), and lead (Pb) in marine specimens of the Baltic and the North Sea.     

Determination of lindane leachability in soil-biosolid systems and its bioavailability in wheat plants

The leachability of lindane from different biosolid amended soils was determined and compared to its bioavailability. Sand, soil, and a mixture of soil-sand (1:1 w/w) were spiked with lindane, blended with different amounts of biosolids, and subjected to a leaching process with water that lasted for 1-28 d. This procedure is in accordance with ISO/TS 21268-1: 2007. After these batch tests, lindane was extracted from the leachates using three different solvent-free microextraction techniques, including solid phase microextraction (SPME), stir-bar sorptive extraction (SBSE), and silicone rod extraction (SRE). The amount of lindane was determined with thermal desorption and gas chromatography coupled to mass spectrometry (GC-MS). The efficiencies of the three microextraction techniques were statistically different, and the efficiency could be related to the amount of polydimethylsiloxane (PDMS) in each extraction device. However, all of the techniques provide data that shows that the leachability of lindane is dependent on the amount of organic matter contained in the matrix.The results of the lindane leachability assay were compared to the bioavailability of lindane, which was determined by measuring the amount of lindane that accumulated in the roots of wheat plants grown in similar soil-biosolid systems.It was confirmed that the amount of organic matter in the matrix is a determining factor for lindane immobilization. The presence of biosolids decreases the mobility of lindane in all of the systems under study. Similarly, increasing biosolid concentrations in the soil significantly decreased the bioavailability of lindane and, consequently, plant absorption.The good correlation (R² = 0.997) between the leachability of lindane from the matrix and plant absorption of lindane indicates that the proposed biomimetic methodology can predict the bioavailability of lindane in a time period as short as 7 d.The results of this work confirm that amending contaminated soils with biosolids is beneficial for immobilizing lindane and helps prevent the percolation of lindane through the soil profile and into groundwater.     

Nitrogen concentrations in mosses indicate the spatial distribution of atmospheric nitrogen deposition in Europe

In 2005/6, nearly 3000 moss samples from (semi-)natural location across 16 European countries were collected for nitrogen analysis. The lowest total nitrogen concentrations in mosses (<0.8%) were observed in northern Finland and northern UK. The highest concentrations (≥1.6%) were found in parts of Belgium, France, Germany, Slovakia, Slovenia and Bulgaria. The asymptotic relationship between the nitrogen concentrations in mosses and EMEP modelled nitrogen deposition (averaged per 50 km × 50 km grid) across Europe showed less scatter when there were at least five moss sampling sites per grid. Factors potentially contributing to the scatter are discussed. In Switzerland, a strong (r² = 0.91) linear relationship was found between the total nitrogen concentration in mosses and measured site-specific bulk nitrogen deposition rates. The total nitrogen concentrations in mosses complement deposition measurements, helping to identify areas in Europe at risk from high nitrogen deposition at a high spatial resolution.     

Response of phase II detoxification enzymes in Phragmites australis plants exposed to organochlorines

Mixed pollution is a characteristic of many industrial sites and constructed wetlands. Plants possessing an enzymatic detoxifying system that is able to handle xenobiotics seems to be a viable option for the removal of mixed persistent contaminants such organochlorines (OCs: monochlorobenzene (MCB), 1,4-dichlorobenzene (DCB), 1,2,4-trichlorobenzene (TCB), γ-hexachlorocyclohexane (HCH)). In this study, Phragmites australis plants were exposed to sub-lethal concentrations of OCs (7 days), in single-exposure (0.8 to 10 mg?l^?1) and in mixture of OCs (0.2 mg?l^?1 MCB?+?0.2 mg?l^?1 DCB?+?2.5 mg?l^?1 TCB?+?0.175 mg?l^?1 HCH). Studies were conducted on the detoxification phase II enzymes; glutathione S-transferases (GST), and glucosyltransferases (UGT). Measurements of GST and UGT activities revealed that OCs may be buffered by glutathione and glucose conjugation. There appeared to be a correlation between the effects on phase II enzymes and the degree of chlorination of the benzene ring with, for example, the greatest effects being obtained for HCH exposure. In the case of mixed pollution, the induction of some GST isoenzymes (CDNB, 35 % non-significant) and UGT (118 %) in leaves and the inhibition of phase II enzymes in the other organs were measured. UGTs appear to be key enzymes in the detoxification of OCs.     

Epilithic Lichen—Atmospheric Deposition Monitors of Trace Elements and Organohalogens?

ABSTRACT

Epilithic lichen (Xanthoria elegans in Canada, Lecanora muralis in Germany) were gathered from 17 locations in Ontario, Canada (from Lake Ontario to James Bay) and 43 locations in Germany (from the Alps to the North Sea and from the Baltic Sea to the Erzgebirge). Sample aliquots were digested in nitric acid and trace elements were analyzed by inductively coupled plasma-mass spectrom-etry techniques. The organohalogens were determined as absorbable organic halogens (AOXs) by coulometry. Concentration ranges from Germany for trace element samples and AOXs were determined. The lichen showed high spatial resolution in their element enrichment patterns. This allowed for differentiation between natural and anthropogenic dominance in ambient air concentrations. This biomonitoring method has proven to be very sensitive, fast, and reliable. No clear relationship could be found between trace element and AOX concentrations. The AOX values may reflect individual metabolic rates of the fungal partner in lichen symbionts.