Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the γ-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities (98%) to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application. 相似文献
Zoige wetland is one of the most important methane emission centers in China. The oxidation of methane in the wetland a ects
global warming, soil ecology and atmospheric chemistry. Despite their global significance, microorganisms that consume methane in
Zoige wetland remain poorly characterized. In this study, we investigated methanotrophs diversity in soil samples from both anaerobic
site and aerobic site in Zoige wetland using pmoA gene as a molecular marker. The cloning library was constructed according to
the pmoA sequences detected. Four clusters of methanotrophs were detected. The phylogenetic tree showed that all four clusters
detected were a liated to type I methanotrophs. Two novel clusters (cluster 1, cluster 2) were found to relate to none of the recognized
genera of methanotrophs. These clusters have no cultured representatives and reveal an ecological adaptation of particular uncultured
methanotrophs in Zoige wetland. Two clusters were belonging to Methylobacter and Methylococcus separately. Denaturing gradient
gel electrophoresis gel bands pattern retrieved from these two samples revealed that the community compositions of anaerobic soil and
aerobic soil were di erent from each other while anaerobic soil showed a higher metanotrophs diversity. Real-time PCR assays of the
two samples demonstrated that aerobic soil sample in Zoige wetland was 1.5 times as much copy numbers as anaerobic soil. These data
illustrated that methanotrophs are a group of microorganisms influence the methane consumption in Zoige wetland. 相似文献
An axial symmetry augmented vertical axis wind turbine, which is suitable for arbitrary wind directions, is proposed in this paper. In order to improve the power generation ability of the S-type vertical axis wind turbine, a set of so-called “collection-shield boards” are installed symmetrically around the rotating S-type rotor. The flow fields around this type of wind turbine are numerically simulated with the aid of CFD method. The optimized design of geometrical parameters of the rotor and collection-shield boards is conducted by using the orthogonal design method. The obtained results suggest that the power output of the optimized augmented wind turbine can reach nearly three times higher than that of the conventional S-type vertical axis wind turbine. 相似文献
本文作者主要研究了腐殖酸对聚乙烯吡咯烷酮包覆的纳米银颗粒(polyvinylpyrrolidone-coated AgNPs)毒性的影响,受试生物涵盖了水生系统不同的营养级别,包括藻类(Raphidocelis subcapitata)、水蚤类(Chydorus sphaericus)以及淡水鱼类(Danio rerio)。结果显示,腐殖酸可降低AgNPs对本研究中所有水生生物的毒性,并具有明显的剂量效应关系。原因为:1)腐殖酸使AgNPs表面带有更多负电荷,这阻碍了AgNPs与藻细胞的接触,使毒性降低;2)腐殖酸抑制了AgNPs中Ag+的溶出,而本研究显示自由Ag+的毒性高于团聚的纳米银颗粒。
精选自Zhuang Wang, Joris T.K. Quik, Lan Song, Evert-Jan Van Den Brandhof, Marja Wouterse and Willie J.G.M. Peijnenburg. Humic substances alleviate the aquatic toxicity of polyvinylpyrrolidone-coated silver nanoparticles to organisms of different trophic levels. Environmental Toxicology and Chemistry: Volume 34, Issue 6, pages 1239–1245, June 2015.
DOI: 10.1002/etc.2936
详情请见http://onlinelibrary.wiley.com/doi/10.1002/etc.2936/full 相似文献
Ambient PM2.5 samples were collected at four sites in Xiamen, including Gulangyu (GLY), Hongwen (HW), Huli (HL) and Jimei (JM) during January, April, July and October 2013. Local source samples were obtained from coal burning power plants, industries, motor vehicles, biomass burning, fugitive dust, and sea salt for the source apportionment studies. The highest value of PM2.5 mass concentration and species related to human activities (SO42–, NO3–, Pb, Ni, V, Cu, Cd, organic carbon (OC) and elemental carbon (EC)) were found in the ambient samples from HL, and the highest and lowest loadings of PM2.5 and its components occurred in winter and summer, respectively. The reconstructed mass balance indicated that ambient PM2.5 consisted of 24% OM (organic matter), 23% sulfate, 14% nitrate, 9% ammonium, 9% geological material, 6% sea salt, 5% EC and 10% others. For the source profiles, the dominant components were OC for coal burning, motor vehicle, biomass burning and sea salt; SO42– for industry; and crustal elements for fugitive dust. Source contributions were calculated using a chemical mass balance (CMB) model based on ambient PM2.5 concentrations and the source profiles. GLY was characterized by high contributions from secondary sulfate and cooking, while HL and JM were most strongly affected by motor vehicle emissions, and biomass burning and fugitive dust, respectively. The CMB results indicated that PM2.5 from Xiamen is composed of 27.4% secondary inorganic components, 20.8% motor vehicle emissions, 11.7% fugitive dust, 9.9% sea salt, 9.3% coal burning, 5.0% biomass burning, 3.1% industry and 6.8% others.
Maintaining soil fertility, while controlling pollution from excessive chemical fertilizer application is important for keeping soil productivity of sustainable agriculture. Variety of straws have been used and proven to be good soil amendments for increasing soil organic matter (OM) and a range of additional soil nutrients. However, little is known about the utilization of cotton straw for soil amendment. To better understand the mechanism behind cotton straw soil amendments, investigations were performed upon cucumber seedlings, where changes to soil nutrients and microbial communities were investigated. The results revealed that the cotton straw application promoted the cucumber seedling growth by significantly increasing the soil OM, available nitrogen, available phosphorus, and available potassium. The concentration of cotton straw was positively correlated to both the number of the culturable microorganisms and also the total microbial biomass within soil. Furthermore, assessment of cotton straw application using Biolog metabolic profiling and phospholipid fatty acid analysis revealed that such application increased the microbial community metabolic activity, and markedly changed the structure of microbial community. 16S rRNA gene clone library construction and phylogenetic analysis of soil bacteria revealed γ- Proteobacteria sequences dominated the cotton straw amendment soil, comprising 27.8% of the total number of analyzed sequences, while they were less represented in control soil (13.4%). On the contrary, the Sphingobacteria (7.8%) and Verrucomicrobia (2.4%) in the cotton straw amendment soil decreased after application when compared to the control soil 15.2% and 15.2%. 相似文献