Supplemental UV-B radiation induced changes in growth, pigments and antioxidant pool of bean (Dolichos lablab) under field conditions

Present study is conducted to evaluate the response of bean (Dolichos lablabcv . pusa early prolific) plants to supplemental UV-B (sUV-B: 280-315 nm: 7.2 kJ m(-2) d(-1)) radiation. UV-B caused alteration in biomass translocation pattern with more retention of biomass in below ground parts leading to an increment in root shoot ratio. Specific leaf area (SLA) which is the measure of leaf thinness, increased in plants under sUV-B exposure by 95.7 and 82.3% after 15 and 30 days after germination. Photosynthetic machinery of bean plants was the potential target of UV-B as photosynthetic rate was decreased by 88.6% at 30 days after germination. sUV-B lead to the formation of reactive oxygen species thus generating oxidative stress. Stimulation of antioxidant defense system (enzymatic and non-enzymatic) was observed due to sUV-B radiation. Phenolic content decreased (34.7 and 18.6%) but protein showed varied response, increased initially (34%) thereafter declined (10.2%) under sUV-B radiation.     

Environmental degradation of the Obra-Renukoot-Singrauli Area,India, and its impact on natural and derived ecosystems

Summary Vast stretches of the Obra—Renukoot—Singrauli region were once covered with natural forests. The tribal dwellings were small and interspersed. However, there has been rapid industrialisation in recent years. Quarrying for limestone, the establishment of a cement factory, thermal power stations and the construction of the G.B. Pant Sagar reservoir have resulted in a rapid build up of human population, the displacement of the original population, deforestation and conversion of natural forest ecosystems into savanna and marginal croplands. The converted ecosystems are under immense biotic stresses such as lopping, grazing, etc.The rainfall is meagre and erratic, the soils are highly weathered and impoverished, consequently the natural forests, as well as the derived ecosystems, are fragile. Signs of desertification are widespread. A rapid depletion in the wildlife has occurred. The establishment of thermal power stations and chemical and cement factories has also resulted in large scale gaseous air pollution, particularly of SO₂ and HF, pollution due to particulate matter through fly ash and cement dust, and that due to liquid effluents. Surface coal mining has caused extensive damage to the natural ecosystems with growing dumps of overburden. The latter needs to be stabilised.This integrated research project on environmental degradation and its impact on air, soil, water and vegetation was divided into five sub-projects, each supervised by a principal investigator. These sub-projects were: (i) Industrial air pollutants and their effects on ecosystem structure and function; (ii) Analysis of structure and functioning of forest ecosystems; (iii) Analysis of structure and functioning of savanna and agroecosystems; (iv) Analysis of structure and functioning of watershed and water-bodies; and (v) Impact of land-use system, socio-economic- demographic conditions, and environmental perception and behavioural management. In these studies dynamics of plant biomass, productivity and nutrients, and biochemical—physiological responses of plants to pollution were emphasised. Environmental perceptions of the native and displaced populations were also studied.The paper outlines a range of recommendations which should help to improve the environmental quality of the region.This article summarises a Man and the Biosphere research project which was sponsored by the Ministry of Environment and Forests of the Government of India. The team of principal investigators comprised Professors J.S. Singh, D.N. Rao, R.S. Ambasht, K.P. Singh, B.S. Gupta and M. Agrawal. The study was coordinated by Professor J.S. Singh, Professor of Botany at Banaras Hindu University.     

Responses of two cultivars of Trifolium repens L. to ethylene diurea in relation to ambient ozone

Three ethylene diurea(EDU) concentrations(0,150 and 300 mg/L) were used to evaluate the negative impact of ozone(O3) on two cultivars of Trifolium repens L.cv.Vardan and Bundel grown under natural field conditions in a suburban area of Varanasi,India.Mean O3 concentrations varied from 30.3 to 46.6 μg/L during the experimental period.Higher photosynthetic pigments and ascorbic acid concentrations were noticed in both EDU-treated cultivars over non-EDU-treated ones,but a reverse trend was found for lipid peroxidation.Growth parameters and biomass also showed increments under EDU treatment of both cultivars.The ratio of variable fluorescence to maximum fluorescence increased significantly in Vardan but not in Bundel upon EDU treatment.Results revealed that EDU concentration of 300 mg/L was more effective to combat the oxidative stress as well as protecting plants from O3 injury symptoms.The test cultivar Vardan is relatively sensitive to O3,thereby can be used as a bioindicator of O3 pollution in areas having higher O3 concentrations.Results also indicated that Bundel has more efficient antioxidant defense system than Vardan and hence was more tolerant to O3 stress.     

Location wise comparison of mixture distributions for assessment of wind power potential: A parametric study

Scientific literature discussed various types of mixture models and models derived from maximum entropy principle using short-term wind speed data for their relative assessment. The literature on suitability of these mixture models for long-term data is rarely available. However, for correct assessment of wind power potential both wind speed and wind direction are equally important. Therefore, in this paper, both wind speed and wind direction are simultaneously analyzed using several types of mixture distribution and compared the same with conventional Weibull distribution. For wind speed and wind power density assessment, the mixture distributions such as Weibull--Weibull distribution, Gamma--Weibull distribution, Truncated Normal--Weibull distribution, Truncated Normal--Normal distribution, proposed Truncated Normal--Gamma distribution and Gamma--Gamma distribution along with MEP-distribution are compared with conventional 2-parameter Weibull distribution. Similarly, for wind direction analysis, the finite mixtures of von-Mises distribution are compared with conventional von-Mises distribution. Judgment criteria include R², RMSE, Kolmogorov--Smirnov test and relative percentage error in wind power density. The sites selected are the three onshore locations of India, viz., Calcutta, Trivandrum, and Ahmedabad. The results show that for wind speed assessment, mixture distribution performs better than the conventional Weibull distribution for analyzing wind power density. However, location wise comparison of all mixture distribution is of prime importance. For wind direction analysis, finite mixture of two von-Mises distributions proved to be a suitable candidate for Indian climatology.     

Use of ethylene diurea (EDU) in assessing the impact of ozone on growth and productivity of five cultivars of Indian wheat (Triticum aestivum L.)

Increase in concentrations of tropospheric ozone (O(3)) is one of the main factors affecting world agriculture production. Tropical countries including India are at greater risk due to their meteorological conditions (high solar radiation and temperature) being conducive to the formation of O(3). The most effective anti-ozonant chemical is N-[2-(2-oxo-1-imidazolidinyl) ethyl]-N-phenylurea or ethylene diurea (EDU). Due to its specific characteristics, EDU has been used in the field as a phytomonitoring agent to assess crop losses due to O(3). Field experiments were conducted on five local cultivars of wheat (Triticum aestivum L. cv HUW234, HUW468, HUW510, PBW343, and Sonalika) grown under natural field conditions in a suburban area of Varanasi, Uttar Pradesh, India during December 2006 to March 2007 to determine the impact of O(3) on their growth and yield characteristics. Mean monthly O(3) concentrations varied between 35.3 ppb and 54.2 ppb at the experimental site. EDU treatment positively affected various growth and yield parameters with difference between cultivars. EDU-treated plants showed increase in shoot and root length, leaf area, absolute growth rate, relative growth rate, and net primary productivity, indicating O(3) induced suppression in growth. EDU treatment was highly significant in different cultivars for total biomass and test weight but not for harvest index. Yield per plant was higher by 25.6%, 24%, 20.4%, 8.6%, and 1.9% in EDU-treated cultivars HUW468, Sonalika, HUW510, HUW234, and PBW343, respectively, than non-EDU-treated ones. These results clearly indicate the sensitivity of all the wheat cultivars to ambient levels of O(3) with cv HUW468 appearing to be most sensitive. The present study also supports the view that EDU has great potential in alleviating the unfavorable effects of O(3) and can be effectively used as a monitoring tool to assess growth and yield losses in areas experiencing elevated concentrations of O(3).     

Effect of long-term application of treated sewage water on heavy metal accumulation in vegetables grown in Northern India

Use of industrial and wastewater for irrigation is on the rise in India and other developing countries because of scarcity of good-quality irrigation water. Wastewaters contain plant nutrients that favour crop growth but leave a burden of heavy metals which can enter the food chain and is a cause of great concern. The present study was undertaken on the long-term impact of irrigation with treated sewage water for growing vegetables and the potential health risk associated with consumption of such vegetable. Treated sewage water (TSW), groundwater (GW), soil and plant samples were collected from peri urban vegetable growing areas of Northern India (Varanasi) and analysed to assess the long-term effect of irrigation with TSW on Cd, Cr, Ni and Pb build-up in soils and its subsequent transfer into commonly grown vegetable crops. Results indicate that TSW was richer in essential plant nutrients but contained Cd, Cr and Ni in amounts well above the permissible limits for its use as irrigation water. Long-term application of TSW resulted in significant build-up of total and DTPA extractable Cd, Cr, Ni and Pb over GW irrigated sites. TSW also resulted in slight lowering in pH, increase in organic carbon (1.6 g kg^− 1) and cation exchange capacity (5.2 cmol kg^− 1). The tissue metal concentration and relative efficiency of transfer of heavy metals from soil to plant (transfer factor) for various groups of vegetables were worked out. Radish, turnip and spinach were grouped as hyper accumulator of heavy metals whereas brinjal and cauliflower accumulated less heavy metals. Health risk assessment by consumption of vegetables grown with TSW indicated that all the vegetables were safe for human consumption. However, significant accumulation of these heavy metals in soil and plant needs to be monitored.     

Impact of Coal Power Plant Emission on the Foliar Elemental Concentrations in Plants in a Low Rainfall Tropical Region

A field study was conducted around two thermal power plants of India to quantify the changes in foliar elemental concentrations due to emission in a low rainfall tropical area. Sulphur dioxide and particulates were at high levels which may cause serious ecological effects. Emission from the power plants has altered the elemental concentrations in the leaves of evergreen and deciduous plants. The foliar total-S and SO₄ ^2–-S levels were significantly higher in all the plants growing at polluted sites. However, the organic-S content was more or less unaltered. In evergreen plants, the SO₄ ^2–-S content increased gradually from summer through winter, whereas in the deciduous plants there was a higher magnitude of increase after onset of new leaves during summer. With the increasing pollution load, foliar Ca²⁺ and K⁺ contents increased, whereas N content decreased. The reduction in N content was greater during summer in evergreen plants and during winter in deciduous plants. The plants growing closer to the power plants accumulated more trace elements (Mn, Fe, Cd, Pb and Ni) as compared to those growing at distant sites. In deciduous plants the leaf fall during winter lowered down the trace element     

Evaluation of physiological, growth and yield responses of a tropical oil crop (Brassica campestris L. var. Kranti) under ambient ozone pollution at varying NPK levels

A field study was conducted to evaluate the impact of ambient ozone on mustard (Brassica campestris L. var. Kranti) plants grown under recommended and 1.5 times recommended NPK doses at a rural site of India using filtered (FCs) and non-filtered open top chambers (NFCs). Ambient mean O₃ concentration varied from 41.65 to 54.2 ppb during the experiment. Plants growing in FCs showed higher photosynthetic rate at both NPK levels, but higher stomatal conductance only at recommended NPK. There were improvements in growth parameters and biomass of plants in FCs as compared to NFCs at both NPK levels with higher increments at 1.5 times recommended. Seed yield and harvest index decreased significantly only at recommended NPK in NFCs. Seed quality in terms of nutrients, protein and oil contents reduced in NFCs at recommended NPK. The application of 1.5 times recommended NPK provided protection against yield loss due to ambient O₃.     

A comparison of North American and Asian exposure–response data for ozone effects on crop yields

Modelling-based studies to assess the extent and magnitude of ozone (O₃) risk to agriculture in Asia suggest that yield losses of 5–20% for important crops may be common in areas experiencing elevated O₃ concentrations. These assessments have relied on European and North American dose–response relationships and hence assumed an equivalent Asian crop response to O₃ for local cultivars, pollutant conditions and climate. To test this assumption we collated comparable dose–response data derived from fumigation, filtration and EDU experiments conducted in Asia on wheat, rice and leguminous crop species. These data are pooled and compared with equivalent North American dose–response relationships. The Asian data show that at ambient O₃ concentrations found at the study sites (which vary between ～35–75 ppb 4–8 h growing season mean), yield losses for wheat, rice and legumes range between 5–48, 3–47 and 10–65%, respectively. The results indicate that Asian grown wheat and rice cultivars are more sensitive to O₃ than the North American dose–response relationships would suggest. For legumes the scatter in the data makes it difficult to reach any equivalent conclusion in relative sensitivities. As such, existing modelling-based risk assessments may have substantially underestimated the scale of the problem in Asia through use of North American derived dose–response relationships.     

Assessment of competitive ability of two Indian wheat cultivars under ambient O3 at different developmental stages

The concentrations of O₃ are increasing, which may have potential adverse effects on crop yield. This paper deals with assessing the intraspecific variability of two wheat cultivars (PBW 343 and M 533) at different growth stages using open top chambers. Mean O₃ concentrations were 50.2 and 53.2 ppb, and AOT40 values were 9 and 12.1 ppm h, respectively, in 2008–2009 and 2009–2010. Reproductive stage showed higher AOT40 values (6.9 and 9.2 ppm h) compared to vegetative (2.23 and 2.9 ppm h). Critical levels of a 3-month AOT 40 of 3 ppm h led to 6 % yield reduction in two wheat cultivars for two consecutive years. Variations in photosynthesis rate, stomatal conductance (g_s), Fv/Fm ratio, photosynthetic pigments, primary and secondary metabolites, morphological parameters, and yield attributes were measured at vegetative and reproductive stages. Reductions in number of leaves, leaf area, total biomass, root/shoot ratio, RGR, photosynthetic pigments, protein content, and Fv/Fm ratio in PBW 343 were more than M 533 at reproductive stage. Photosynthetic rate did not vary between the cultivars, but g_s was higher in PBW 343 compared to M 533 under ambient O₃. Higher total phenolics and peroxidase activity were recorded in M 533 at reproductive stage conferring higher resistance at latter age. Results of O₃ resistance showed that M 533 was sensitive compared to PBW 343 during vegetative stage but developed more resistance at reproductive stage. PBW 343 with larger leaf area and high g_s is more sensitive than M 533 with smaller leaf area and low g_s. The study suggests that the sensitivity varied with plant growth stage, and the plant showing higher sensitivity during vegetative period developed more resistance during reproductive period due to higher defense mechanism. Though the yield reductions were same in both cultivars under ambient O₃, the mechanism of acquiring the resistance is different between the cultivars.