High frequency of extra-pair paternity in Swedish pied flycatchers revealed by allozyme electrophoresis and DNA fingerprinting

Summary We used two genetic techniques to study multiple parentage in the pied flycatcher (Ficedula hypoleuca). Allozyme electrophoresis of 131 nestlings from 22 clutches sampled in 1982 and 1987 showed that one nestling had a mismatched allele compared with its putative parents. In one additional nest the devitation from Mendelian inheritance of parental genotypes suggested extra-pair paternity. The calculated probability of detecting multiple paternity from the genetic variation at four loci was 0.115. The estimated population frequency of extra-pair fertilization (EPF) was 13%, based on two mismatches and the probability of detecting multiple paternity. The seven families (n = 38 nestlings) in the 1987 sample were also analyzed by DNA fingerprinting using the M13 and Jeffrey's 33.15 probes. Overall, 24% (n = 9) of the nestlings analyzed were genetically inconsistent with their putative father, with EPF occurring in three (43%) of the seven clutches. One nestling originated by intraspecific brood parasitism. Comparison of the two techniques using the same samples showed that allozyme electrophoresis has a resolution only 11% of that of DNA fingerprinting, close to the calculated probability of detecting multiple paternity (0.115). Both techniques suggest that extra-pair fertilization is relatively common in the Swedish populations investigated compared to the low frequency reported from a Norwegian population. Correspondence to: H.P. Gelter at the present address     

Mate choice in the willow warbler — a field experiment

Summary Females may choose a mate on his own quality or on the quality of his resources, i.e., his territory. We removed willow warbler males and allowed new males to settle, before the arrival of females, in order to test whether the proximate cue for female choice was any male trait or territory characteristics. The experiment indicates that females base their choice on some male trait. No correlation was found between male settlement order and size (tarsus length, wing length), but males arriving early were in better body condition than males arriving late. The most likely male trait for female choice was singing rate, which was a good indicator of male settlement date. The duration of time between mating and egg laying was shorter in early territories, both in the observational and the experimental data. Since male song rate was correlated with territory quality (e.g., food abundance) the ultimate benefit to females choosing males with high song rates could be a high quality territory.Offprint requests to: B.L. Arvidsson     

Aufnahme von chlorierten Kohlenwasserstoffen durch Pflanzenoberflächen

Spinach plants were contaminated with hexachlorobenzene (HCB) and 2,2′, 5,5′-tetrachlorobiphenyl (PCB) in the vapor phase and by bentonite aerosol. To characterize the deposition of these substances on contaminated plant leaves, different solvents were used (water,n-hexane, and acetone). The solubility of the substances was shown to depend on the mode of application. Release studies indicate that the concentration of the tested chemicals decreases to 5% for HCB and to 27%, respectively 49%, for PCB after a period of 4 days at 23°C for different modes of contamination.     

Spacing of leks in relation to female home ranges,habitat requirements and male attractiveness in the great snipe (Gallinago media)

Summary In an attempt to examine three main hypotheses on the evolution of leks, data on female home ranges, distance between leks and male site fidelity in the great snipe (Gallinago media) were obtained. In a 30 × 40 km area in central Sweden, the locations of 12 leks were identified. Six were within the study area (7 × 11 km) in which probably all leks were known. In the study area, nearest neighbor distances between leks were longer than the predicted drawing area (diameter of female home ranges plus the detection range of leks). Furthermore, in only 1 of 13 radio-tracked females did the estimated home range enclose many leks. These data do not fit the prediction from the hotspot hypothesis on the dispersion of leks, namely, that the distance between leks should be less than the drawing area and hence an average female home range should enclose more than one lek. The observed distance between leks was about the same as predicted by the female preference hypothesis. This hypothesis also predicts females visit mainly one lek; however, we found they sometimes visit two and nest close to a third. We propose that males may first settle according to hotspot rules, but females will resettle according to preferences for certain males and/or larger leks. In this way some hotspot leks are abandoned, and the distance between leks is increased as males become more tightly clumped. However, when all males and females have settled, the leks still existing would be on hotspots. In the female preference hypothesis, males are assumed to aggregate on leks because females prefer clustered males as they can be more easily compared than if they are dispersed. Alternatively, in the attractiveness hypothesis, it is suggested that females prefer certain males, and hence unattractive males surround the attractive are forced to join leks in order to come close to females. Our data on male site fidelity support the attractiveness hypothesis because successful and dominant males return to the same lek and territory, both within and between years, whereas unsuccessful males move to other leks. Attractiveness of certain males may explain why according to hotspot rules in this species males are more clumped than expected. Offprint requests to: J. Höglund     

Die Ektoplasmahülle von Heterostegina depressa (Foraminifera:Nummulitidae)

The structure of the rigid ectoplasm sheath was studied in living individuals of the large foraminifer Heterostegina depressa d'Orbigny 1826 (Nummulitidae). H. depressa is able to form pseudopods, which emerge from the marginal cord within a few minutes. Normally, however, the living test is entirely covered by a rigid hyalin elastic sheath, which is fixed to the substrate by terminally branched processes. In older individuals, the sheath is often incomplete and restricted to the marginal cord only. Probably, the sheath is secreted by the ectoplasm which fills the canal system of the chamber walls and the marginal cord, or it consists of transformed ectoplasm; we call it “Ektoplasmahülle”, and the processes radiating to all sides, “Ruhepseudopodien”. H. depressa is able to move while inside the elastic sheath by means of pseudopods which penetrate the sheath and attach themselves to the substrate out-side. In the same manner, H. depressa leaves a complete sheath by disrupting it through the tractive powers of the pseudopods. The procedure of breaking the sheath is described. Locomotion when leaving the sheath is performed in a zigzag manner (alternating action of several pseudopods). The sheath counteracts transportation by water movement and protects chamber formation (which takes place inside the sheath). Due to its symbiotic unicellular algae, H. depressa does not need to collect food particles; hence locomotion seems unneccssary. The formation of a rigid ectoplasm sheath may be connected with H. depressa's sessile mode of life.     

Benthic Foraminifera: morphology and growth in clone cultures of Heterostegina depressa

A three months old clone of 76 asexually produced F₂ Heterostegina depressa D'Obbigny, 1826 (Foraminifera) was subdivided into two groups which were cultivated for 5 months, one at 300 lux, the other at 600 lux. Nourishment was provided by symbiotic photosynthesizing algae. The 300-lux group added 1 whorl, the 600-lux group added 1/2 a whorl during the experiment, suggesting that the first group was nearer the optimum for growth than the second. Growth rates reacted but slowly to changes in environmental conditions. Also, specimens within the same culture dish showed great differences in response. In the 600-lux group, many specimens ceased to grow entirely, while others continued. Cessation of growth tends to produce a rounded test shape, by filling-in of the apertural angle, while rapid growth tends to be correlated with flaring tests (i.e., tests with an open spiral). In addition, there is a trend toward increased flaring during normal ontogenetic development. Shell damage is greater in rapidly growing individuals; walls are apparently thicker in slowly growing and in resting individuals. Ecological interpretation of morphological characteristics is hampered by: (1) a lag effect between growth response and environmental change; (2) slowing or termination of growth under stressful conditions with corresponding minor changes in morphology; (3) great variation in response between sibling individuals within the same culture dish; (4) aging effects.     

Queen fertility, egg marking and colony size in the ant Camponotus floridanus

In ant societies, workers do not usually reproduce but gain indirect fitness benefits from raising related offspring produced by the queen. One of the preconditions of this worker self-restraint is sufficient fertility of the queen. The queen is, therefore, expected to signal her fertility. In Camponotus floridanus, workers can recognize the presence of a highly fertile queen via her eggs, which are marked with the queen's specific hydrocarbon profile. If information on fertility is encoded in the hydrocarbon profile of eggs, we expect workers to be able to differentiate between eggs from highly and weakly fertile queens. We found that workers discriminate between these eggs solely on the basis of their hydrocarbon profiles which differ both qualitatively and quantitatively. This pattern is further supported by the similarity of the egg profiles of workers and weakly fertile queens and the similar treatment of both kinds of eggs. Profiles of queen eggs correspond to the cuticular hydrocarbon profiles of the respective queens. Changes in the cuticular profiles are associated with the size of the colony the queen originates from and her current egg-laying rate. However, partial correlation analysis indicates that only colony size predicts the cuticular profile. Colony size is a buffered indicator of queen fertility as it is a consequence of queen productivity within a certain period of time, whereas daily egg-laying rate varies due to cyclical oviposition. We conclude that surface hydrocarbons of eggs and the cuticular profiles of queens both signal queen fertility, suggesting a major role of fertility signals in the regulation of reproduction in social insects.