环境基质中药物残留的固相提取与分析

废水流出物常常不可避免地进入地表水和用于饮用水资源的含水层．因废水再循环常常进入饮用水层,各种废水中的污染物最终会污染饮用水．以往环保部门一直比较注重农药和工业污染物,但最近的研究发现:废水流出物中的某些药物残留已达到了相当令人关注(１μｇ·１－１即ｐｐｂ)的程度．传统的分析技术(如液液萃取和ＧＣ/ＭＳ) 对极性较弱的化合物(ＰＣＢｓ和许多农药)比较有效,但对于极性相对较强的药物污染物而言,固相提取(ＳＰＥ)与ＬＣ/ＭＳ技术更适合于样品提取分析。     

Noninvasive Stress and Reproductive Measures of Social and Ecological Pressures in Free-Ranging African Elephants

Abstract: The African elephant (   Loxodonta africana ) experienced a poaching-related 60% population decline between 1979 and 1988 that was inordinately concentrated on adults. This, coupled with political pressures to delist the elephant, has created a need for noninvasive physiological measures that can quantify the long-term effects of past mortality patterns of this long-lived species. We collected fresh fecal samples from 16 female elephants in three different groups over 23 months at Tarangire National Park, Tanzania, and analyzed them for fecal progesterone and cortisol metabolites. Social and ecological measures were collected concurrently. Fecal progesterone metabolite measures corresponded significantly with stage of gestation, and appear to be able to confirm pregnancy in female elephants from as early as 3 months of gestation. We found that progesterone metabolite concentrations were significantly lower during the dry season than during the wet season after controlling for stage of gestation. Fecal cortisol metabolite concentrations showed the opposite seasonal pattern, being significantly higher in the dry season and inversely correlated with rainfall across seasons. Fecal cortisol metabolite concentrations also increased with group size and were correlated positively with dominance rank in the largest group. Our results suggest that measures of progesterone and cortisol metabolites in feces provide indices of reproductive function and physiological stress that can quantify both natural and human disturbances in African elephants. These measures are ideally suited for monitoring the long-term effects of social disruption from poaching and a variety of other management concerns.     

A Systematic Test of an Enterprise Strategy for Community-Based Biodiversity Conservation

Abstract: A commonly held belief is that if people can benefit financially from enterprises that depend on nearby forests, reefs, and other natural habitats, then they will take action to conserve and sustainably use them. The Biodiversity Conservation Network brought together conservation and development organizations and local communities to systematically test this hypothesis across 39 conservation project sites in Asia and the Pacific. Each project implemented one or more community-based enterprises such as setting up an ecotourism lodge, distilling essential oils from wild plant roots, producing jams and jellies from forest fruits, harvesting timber, or collecting marine samples to test for pharmaceutical compounds. Each project team collected the biological, enterprise, and social data necessary to test the network's hypothesis. We present the results of this test. We found that a community-based enterprise strategy can lead to conservation, but only under limited conditions and never on its own. We summarize the specific conditions under which an enterprise strategy will and will not work in a decision chart that can be used by project managers to determine whether this strategy might make sense at their site. We also found that an enterprise strategy can be subsidized and still create a net gain that pays for conservation. Based on our experiences, we recommend developing "learning portfolios" that combine action and research to test other conservation strategies.     

Chromosomal evolution in Mugilidae: karyotype characterization of Liza saliens and comparative localization of major and minor ribosomal genes in the six Mediterranean mullets

This study continues a comparative cytogenetic analysis of the fish family Mugilidae, reporting the karyotype characterization of the leaping mullet, Liza saliens, by C-banding, Ag- and fluorochrome-staining, and completing the fluorescence in situ hybridization (FISH)-mapping of the 18S and 5S rRNA genes (rDNA) to the chromosomes of the six Mediterranean mullets, namely L. saliens, L. ramada, L. aurata, Mugil cephalus, Chelon labrosus and Oedalechilus labeo. In all species, except M. cephalus, the 5S rDNA sites were localized on a medium-sized acrocentric chromosome pair, which was considered homeologous in all of them. In L. saliens, an additional 5S rDNA site was detected in a location close to the one shown by major ribosomal genes in M. cephalus, i.e. the subtelomeric region of chromosome pair 1. The 5S rDNA site in M. cephalus is located on the smallest chromosome pair of the complement, which, on the other hand, though on a different position, bears 18S rDNA in all the species of Liza and Chelon examined. The heterochromatin composition and the major and minor ribosomal gene locations suggest that the karyotype of L. saliens (subgenus Protomugil) can be considered intermediate between the karyotype of the more primitive M. cephalus and those of the other Liza (subgenus Liza) species and of the representatives of the more derived genera Chelon and Oedalechilus.     

Environmental levels of sediment-associated tri-n-butyltin chloride (TBTCl) and ionic regulation in flounders during seawater adaptation

The effects of exposure to sediment-associated tri-n-butyltin chloride (TBTCl) were examined in the euryhaline European flounder, Platichthys flesus (L.). The effects were quantified by measuring the changes in sodium efflux; Na⁺/K⁺-ATPase activity; and the numbers, areas, and distribution of chloride cells in the gills of freshwater-adapted fish, following a rapid transfer to seawater. Following the transfer to seawater, the Na⁺/K⁺-ATPase activity and the sodium efflux were significantly increased in the control group but remained unchanged in the TBTCl-exposed group. The normal morphological changes to the gill epithelium associated with seawater adaptation, which involve chloride cell distribution, took place in the control group but were significantly inhibited or delayed in the TBTCl group. The results presented in this study lead to the conclusion that environmental concentrations of tri-n-butyltin chloride in sediments are capable of significantly disrupting both the physiological and the morphological components of iono-regulatory functions of an estuarine flatfish.     

Feeding, growth and reproduction of sea urchins (Strongylocentrotus droebachiensis) on single and mixed diets of kelp (Laminaria spp.) and the invasive alga Codium fragile ssp. tomentosoides

Since its introduction to Nova Scotia in the early 1990s, the invasive green alga Codium fragile ssp. tomentosoides has spread rapidly to become a dominant and persistent component of seaweed assemblages in the rocky subtidal zone. To examine the potential of sea urchins (Strongylocentrotus droebachiensis) to regulate Codium, and the potential of the alga to support urchin growth and reproduction, we fed urchins Codium and/or kelp (Laminaria spp., a high quality and preferred native food) in the laboratory for 11 months. Urchins showed a strong and active preference for kelp: they consumed more Laminaria than Codium (as dry weight) in single and mixed diet treatments. Urchins fed only Codium consumed 3.5 times more of the alga than those on a mixed diet, but did not increase their feeding rate in winter when kelp consumption was greatest. Laminaria was consumed at the same rate in single and mixed diets, indicating that the presence of an alternative food had no effect on kelp consumption. Survival and growth (change in test diameter) were lowest on the Codium diet, although the differences among diets were not statistically significant. Urchins on the Codium diet showed no gonadal production over the annual reproductive cycle, compared to a marked rise in gonad index on the Laminaria and mixed diets. Our results indicate that Codium is an unattractive, unpalatable and poor quality food, which is not readily consumed by urchins. Thus we predict that urchins at moderate densities will be much more likely to graze kelp than Codium in mixed stands, creating a mosaic of Codium patches and barren areas. At high densities, however, urchins are likely to destructively graze all seaweeds, although dense stands of the invasive alga may prolong the widespread formation of barrens.     

Phylogenetic relationships within the Octocorallia (Cnidaria: Anthozoa) based on nuclear 18S rRNA sequences

We determined the nuclear 18S rRNA sequences for 41 species of octocorals and used these to address the validity of the historical ordinal divisions and the current subordinal divisions within the subclass Octocorallia. We also explored the phylogenetic affinities of the species Dendrobrachia paucispina, which was originally classified in the order Antipatharia (subclass Ceriantipatharia) although polyp structure indicates it belongs in the subclass Octocorallia. Trees constructed using maximum likelihood techniques are incongruent with the current and historical taxonomy of the Octocorallia. There appeared to be three major clades of octocorals. The first clade included most, but not all, pennatulaceans as a monophyletic group. The second clade contained 21 species, representing all major octocoral groups other than pennatulaceans. The third clade contained members from three suborders of the Alcyonacea and one member of the Pennatulacea. These data could not be used to distinguish the branching order of the three major clades. The species D. paucispina had a close affinity with the genera Corallium and Paragorgia (Alcyonacea: Scleraxonia), although its morphology suggests it is more similar to the genus Chrysogorgia (Alcyonacea: Calcaxonia). The morphological character of dimorphism (the presence of both autozooids and siphonozooids within a single colony) corresponded loosely with the topology of the most likely trees, and a single origin of dimorphism could not be rejected. Despite sampling from the majority of families within the Octocorallia, many of the relationships within this group remain ambiguous. Received: 16 June 2000 / Accepted: 14 September 2000     

Three-Dimensional Modelling of Concentration Fluctuations in Complicated Geometry

The strong fluctuating component in the measured concentration time series of a dispersing gaseous pollutant in the atmospheric boundary layer, and the hazard level associated to short-term concentration levels, demonstrate the necessity of calculating the magnitude of turbulent fluctuations of concentration using computational simulation models. Moreover the computation of concentration fluctuations in cases of dispersion in realistic situations, such as built-up areas or street canyons, is of special practical interest for hazard assessment purposes. In this paper, the formulation and evaluation of a model for concentration fluctuations, based on a transport equation, are presented. The model is applicable in cases of complex geometry. It is included in the framework of a computational code, developed for simulating the dispersion of buoyant pollutants over complex geometries. The experimental data used for the model evaluation concerned the dispersion of a passive gas in a street canyon between 4 identical rectangular buildings performed in a wind tunnel. The experimental concentration fluctuations data have been derived from measured high frequency concentrations. The concentration fluctuations model is evaluated by comparing the model's predictions with the observations in the form of scatter plots, quantile-quantile plots, contour plots and statistical indices as the fractional bias, the geometrical mean variance and the factor-of-two percentage. From the above comparisons it is concluded that the overall model performance in the present complex geometry case is satisfactory. The discrepancies between model predictions and observations are attributed to inaccuracies in prescribing the actual wind tunnel boundary conditions to the computational code.     

Experimental Study of the Criteria of Flow Laminarization in Two-Dimensional Dense Gas Plumes

Laminarization of flow in a two-dimensional dense gas plume was experimentally investigated in this study. The plume was created by releasing CO₂ through a ground-level line source into a simulated turbulent boundary layer over an aerodynamically rough surface in a meteorological wind tunnel. The bulk Richardson number (Ri_*), based on negative plume buoyancy, plume thickness, and friction velocity, was varied over a wide range so that the effects of stable stratification on plume laminarization could be observed. A variety of ambient wind speeds as well as three different sizes of roughness arrays were used so that possible effects of roughness Reynolds number (Re_*) on plume laminarization could also be identified. Both flow visualization methods and quantitative measurements of velocity and intermittency of turbulence were used to provide quantitative assessments of plume laminarization.Flow visualization provided an overall picture of how the plume was affected by the negative buoyancy. With increasing Ri_*, both the plume depth and the vertical mixing were significantly suppressed, while upstream propagation of the plume from the source was enhanced. The most important feature of the flow revealed by visualization was the laminarization of flow in the lower part of the plume, which appeared to be closely related to both Ri_* and Re_*.Measurements within the simulated dense gas plumes revealed the influence of the stable stratification on mean velocity and turbulence intensity profiles. Both the mean velocity and turbulence intensity were significantly reduced near the surface; and these reductions systematically depended on Ri_*. The roughness Reynolds number also had considerable influence on the mean flow and turbulence structure of the dense gas plumes.An intermittency analysis technique was developed and applied to the digitized instantaneous velocity signals. It not only confirmed the general flow picture within the dense plume indicated by the flow visualization, but also clearly demonstrated the changes of flow regime with variations in Ri_* and Re_*. Most importantly, based on this intermittency analysis, simple criteria for characterizing different flow regimes are formulated; these may be useful in predicting when plume laminarization might occur.     

Identifying the largest individual sample value from a two-way composite sampling design

Composite sampling techniques for identifying the largest individual sample value seem to be cost effective when the composite samples are internally homogeneous. However, since it is not always possible to form homogeneous composite samples, these methods can lead to higher costs than expected. In this paper we propose a two-way composite sampling design as a way to improve on the cost effectiveness of the methods available to identify the largest individual sample value.