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101.
102.
Evaluating seasonal dynamics of bacterial communities in marine fish aquaculture: a preliminary study before applying phage therapy 总被引:1,自引:0,他引:1
Pereira C Salvador S Arrojado C Silva Y Santos AL Cunha A Gomes NC Gomes N Almeida A 《Journal of environmental monitoring : JEM》2011,13(4):1053-1058
The increasing problem of antibiotic resistance in common pathogenic bacteria and the concern about the spreading of antibiotics in the environment bring the need to find new methods to control fish pathogens. Phage therapy represents a potential alternative to antibiotics, but its use in aquaculture requires a detailed understanding of bacterial communities, namely of fish pathogenic bacteria. Therefore, in this study the seasonal dynamics of the overall bacterial communities, microbiological water quality and disease-causing bacteria were followed in a marine aquaculture system of Ria de Aveiro (Portugal). Analysis of the bacterial diversity of the water samples by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments indicates that the bacterial community structure varied seasonally, showing a higher complexity during the warm season. The diversity of the main fish pathogenic bacteria, assessed by DGGE targeting the Vibrio genus, showed lower seasonal variation, with new dominating populations appearing mainly in the spring. Bacterial indicators, faecal coliforms and enterococci, enumerated by the filter-membrane method, also varied seasonally. The fluorescent in situ hybridization (FISH) results showed that the specific groups of bacteria varied during the study period and that the non-indigenous Enterobactereaceae family was the most abundant group followed by Vibrio and Aeromonas. The seasonal variation detected in terms of density and structure of total and pathogenic bacterial communities demonstrates the need for a careful monitoring of water through the year in order to select the suitable phages to inactivate fish pathogenic bacteria. The spring season seems to be the critical time period when phage therapy should be applied. 相似文献
103.
Stoski Adriana Latczuk Izabelli Fiuza Muniz Edvani Curti Almeida Carlos Alberto Policiano 《Journal of Polymers and the Environment》2022,30(7):2955-2963
Journal of Polymers and the Environment - The main objective of this research was to set the experimental conditions needed for the successful synthesis of a composite (PU-UBPFS) made from... 相似文献
104.
Roque António José da Silva Paula F. de Almeida Rui Pedro Marques 《Journal of Material Cycles and Waste Management》2022,24(6):2385-2400
Journal of Material Cycles and Waste Management - A crushed concrete aggregate, processed from construction and demolition waste and a siderurgical aggregate, processed from electric arc furnace... 相似文献
105.
106.
Médice Rhuana Valdetário Afonso Robson José de Cássia Franco Almeida Marys Lene Braga de Aquino Sérgio Francisco Libânio Marcelo 《Environmental science and pollution research international》2021,28(4):3828-3836
Environmental Science and Pollution Research - Among drugs and personal care products, antibiotics arouse interest since they are widely used in human and veterinary medicine and can lead to the... 相似文献
107.
Silvia H. Bonilla Cecília M.V.B. Almeida Biagio F. Giannetti Donald Huisingh 《Journal of Cleaner Production》2010,18(1):1-5
This issue of the Journal of Cleaner Production is based upon papers presented at the 1st International Workshop Advances in Cleaner Production (CP) held in São Paulo, Brazil, in 2007. The conference had the short-term purpose of deepening the Brazilian discussion on “The Roles of Cleaner Production in the Sustainable Development of Modern Societies”, and it had the long-term objective of providing an on-going interdisciplinary forum for knowledge development and exchange on Cleaner Production (CP) and Sustainable Development. This issue is devoted to papers covering a broad range of perspectives of CP practices and strategies. A special focus is placed upon methodological tools designed to support effective decision-making pertaining to quantitative benefits from CP.The ten papers provide insights from research designed to holistically integrate CP to help society make effective progress to sustainability. Papers cover the importance of informal knowledge, as complementary to formal knowledge, in performing effective ‘Environmental Impact Assessments.’ One paper explores the roles of radical and incremental innovation in the context of alternative automotive technologies. Benefits of Ecodesign are explored in two papers; one concerning its integration with remanufacturing to extend the life of used products and one focusing the adoption of ‘Emergy Environmental Accounting,’ as a complementary decision-making tool. The development of the Brazilian LCI database for ‘hydroelectric power generation’ and its contribution to support regionally relevant LCA studies is highlighted in one paper. The complete production chains of biodiesel and bioethanol are evaluated by using global methodologies, which help in the development of more objective and effective solutions. A “compensatory area”, calculated in terms of emergy, is proposed in order to work in a sustainable way for bamboo production. Finally, a paper about a novel approach for recycling used PET is also included. 相似文献
108.
109.
Diane Ciaparra Eric Aries Marie-Jo Booth David R. Anderson Susana Marta Almeida Stuart Harrad 《Atmospheric environment (Oxford, England : 1994)》2009,43(12):2070-2079
Investigations have been undertaken at two integrated steelworks in the UK to characterise airborne organic micro-pollutants and to assess the contribution of iron ore sintering and coke making operations on the air quality. Concentrations of volatile organic compounds (VOCs), namely benzene, toluene and p-xylene, were measured continuously within the boundary of a coking plant using for the first time differential optical absorption spectrometry (DOAS) between 2004 and 2006. Concentrations were obtained along two monitoring paths surrounding the coke plant and the average benzene concentration measured along both paths over the campaign was 28 μg m?3. Highest benzene concentrations were associated with winds downwind of the coke oven batteries. Concentrations of polycyclic aromatic hydrocarbons (PAHs) in ambient air were measured during 27 consecutive days in 2005 at three different locations on an integrated steelworks. PAH profiles were determined for each sampling point and compared to coke oven and sinter plant emission profiles showing an impact from the steelworks. The mean benzo [a] pyrene concentration determined in the immediate vicinity of the coke ovens downwind from the battery was 19 ng m?3, whereas for the two other sites average benzo [a] pyrene concentrations were much lower (around 1 ng m?3). Data were analysed using principal components analysis (PCA) and results showed that coke making and iron ore sintering were responsible for most of the variation in the PAH concentrations in the vicinity of the investigated plant. 相似文献
110.
Rissato SR Galhiane MS Ximenes VF de Andrade RM Talamoni JL Libânio M de Almeida MV Apon BM Cavalari AA 《Chemosphere》2006,65(11):1949-1958
Detailed analyses of persistent organic pollutants (POPs) such as organochlorine pesticides (OCPs), hexachlorocyclohexane (HCH) isomers (HCHs), dichlorodiphenyltrichloro ethane (DDT) and its metabolites (DDTs) and congeners of polychlorinated biphenyls (PCBs) in soil and surface water from the northeastern São Paulo, Brazil allowed the evaluation of the contamination status, distribution and possible pollution sources. The pesticides and PCBs demonstrated markedly different distributions, reflecting different agricultural, domestic and industrial usage in each region studied. The ranges of HCH, DDT, and PCBs concentrations in the soil samples were 0.05–0.92, 0.12–11.01, 0.02–0.25 ng g−1 dry wt, respectively, and in the surface water samples were 0.02–0.6, 0.02–0.58 and 0.02–0.5 ng l−1, respectively. Overall elevated levels of DDT and PCB were recorded in region 2, a site very close to melting, automotive batteries industries, and agricultural practice regions. High ratios of metabolites of DDT to DDT isomers revealed the recent use of DDT in this environment. The sources of contamination are closely related to human activities, such as domestic and industrial discharge, street runoff, agricultural pesticides and soil erosion, due to deforestation as well as atmospheric transport. 相似文献