Euro Chlor Risk Assessment for the Marine Environment Osparcom Region: North Sea - Tetrachloroethylene

This risk assessment on tetrachloroethylene (PER) was carried out specifically for the marine environment, according to the methodology laid down in the EU risk assessment Regulation (1488/94) and the Guidance Document of the EU New and Existing Substances Regulation (TGD, 1997). The study consists of the collection and evaluation of data on effects and environmental concentrations from analytical monitoring programs in large rivers and estuaries in the North Sea area. The risk is indicated by the ratio of the "predicted environmental concentrations" (PEC) and the "predicted no effect concentrations" (PNEC) for the marine aquatic environment. In total, 18 studies for fish, 13 studies for invertebrates and 8 studies for algae have been evaluated. Both acute and chronic toxicity studies have been taken into account and the appropriate assessment factors have been used to define a PNEC value of 51 µg/l. Most of the available monitoring data apply to rivers and estuary waters and were used to calculate PECs. The most recent data (1991-1995) support a typical PEC of 0.2 µg PER/l water and a worst case PEC of 2.5 µg PER/l water. The calculated PEC/PNEC ratios give a safety margin of 20 to 250 between the predicted no effect concentration and the exposure concentration. Additional evaluation of environmental fate and bioaccumulation characteristics showed that no concern is expected for food chain accumulation.     

A Greenhouse Gas Balance of two Existing International Biomass Import Chains

Various utility companies are considering or already initiated the import of biomass from abroad for electricity generation, especially via co-firing in coal-fired power plants. This results in international logistic biomass supply chains, which raise questions on the environmental performance of such chains. In this study, a life cycle inventory has been performed on two existing biomass import chains to evaluate the greenhouse gas balance of biomass import for co-firing. We considered production, transport and co-firing of wood pellets from Canada and palm kernel shells from Malaysia in a 600 MW _e coal-fired power plant in the Netherlands. Those chains are compared with various reference systems for energy production and the alternative use of biomass. Primary energy savings of these import and co-firing chains are between 70% and 100% of the biomass energy content. Net avoided greenhouse gas emissions are in the range of 340–2100 g/kWh. In the most optimistic scenario, pellet co-firing avoids methane emissions that would have occurred if the pellets were decomposed at landfills when not applied for energy production. In the most pessimistic scenario, palm kernel shell co-firing competes with the application as resource for animal feed production, which requires production and transport of an alternative resource. As the energy reference systems of the importing and exporting country and the alternative application of biomass have a significant impact on the net avoided greenhouse gas emissions, these factors should be considered explicitly when studying biomass trade for energy purposes.     

Migration and habitat of white sharks (<Emphasis Type="Italic">Carcharodon carcharias</Emphasis>) in the eastern Pacific Ocean

Satellite telemetry studies of 20 adult and sub-adult white sharks (360–530 cm estimated total length (TL)) in the eastern North Pacific during 1999–2005 revealed long distance seasonal migrations from the coast of California to an offshore focal area 2,500 km west of the Baja Peninsula, as well as the Hawaii Islands. Three tags were recovered allowing detailed behavioral analyses, including one shark’s migration cycle from the coast to the offshore focal area and back. While near pinniped rookeries in autumn and winter, sharks avoided the surface and used water to 50 m depth, consistent with a silhouette-based hunting strategy. Offshore migrations were initiated during November–March and followed periods of decreasing pinniped abundance. Migrations were highly directed, taking 23 ± 5 days to reach the offshore focal area along similar paths among sharks and years, defining a migration corridor. Sharks exhibited a broad depth distribution (0–644 m) in the offshore focal area, and remained there for up to 167 days during spring and summer, though primary productivity and fishery data suggest that forage resources are scarcer there than in other regions of the eastern North Pacific. Archival data from one shark revealed intensive oscillatory movements while in the offshore focal area, a behavior that may be related to foraging or mating. Sharks traveling to Hawaii remained near the islands up to 122 days, potentially feeding on pelagic fishes and marine mammals that concentrate around the islands. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Maternal serum free beta hCG screening: Results of studies including 480 cases of down syndrome

The median maternal serum free beta human chorionic gonadotropin (hCG) multiple of the median (MOM) of 480 Down syndrome cases in the second trimester was 2·64, significantly greater than the reported median MOM of intact hCG (p<0·0001). In 234 of these cases from retrospective and prospective studies, the effectiveness of maternal serum free beta hCG was evaluated in combination with alpha-fetoprotein (AFP) and maternal age in second-trimester Down syndrome screening. Down syndrome detection in the gestational age range of 14–16 weeks was 82 per cent. In all gestational weeks (14–22), a 77·7 per cent Down syndrome detection rate was achieved. In prospective screening of 44 272 patients under the age of 35 years, 69 per cent of Down syndrome cases were detected (73 per cent in gestational weeks 14–16). The false-positive rate for the prospective study was 3·8 per cent. The use of free beta hCG combined with maternal serum AFP and maternal age-related risk for Down syndrome in a screening population (i.e., women under 35 years) yields an improved detection efficiency over other protocols.     

Effects of four decades of fire manipulation on woody vegetation structure in Savanna

The amount of carbon stored in savannas represents a significant uncertainty in global carbon budgets, primarily because fire causes actual biomass to differ from potential biomass. We analyzed the structural response of woody plants to long-term experimental burning in savannas. The experiment uses a randomized block design to examine fire exclusion and the season and frequency of burn in 192 7-ha experimental plots located in four different savanna ecosystems. Although previous studies would lead us to expect tree density to respond to the fire regime, our results, obtained from four different savanna ecosystems, suggest that the density of woody individuals was unresponsive to fire. The relative dominance of small trees was, however, highly responsive to fire regime. The observed shift in the structure of tree populations has potentially large impacts on the carbon balance. However, the response of tree biomass to fire of the different savannas studied were different, making it difficult to generalize about the extent to which fire can be used to manipulate carbon sequestration in savannas. This study provides evidence that savannas are demographically resilient to fire, but structurally responsive.     

Assessing the organic composition of urban surface films using nuclear magnetic resonance spectroscopy

Recently it has been shown that urban surfaces are covered with a thin film which mediates the fate, distribution and accumulation of semi-volatile organic compounds in the environment. In this study we apply a combination of solution, semi-solids, and solid state nuclear magnetic resonance (NMR) methods to provide a general overview of the organic constituents. In surface film collected from 30 m2 of outside windows over an area of 12 km2 in downtown Toronto, we roughly estimate that the organic carbon is approximately 35% carbohydrate, approximately 35% aliphatics, approximately 20% aromatics, and approximately 10% carbonyl groups. Various aliphatic groups can be identified including a number of acids, alcohols, alkanes, and alkenes. Also, numerous intact aliphatic esters are apparent that have not been observed before, as well as carbohydrates. The aromatic species include a small portion that appears to be derived from a polymer of styrene, in addition a larger fraction is consistent with polyhydroxylated PAH derived material, although this assignment is tentative and based solely on 1-D NMR data only. In addition, signals from polybutadiene are present and while accurate quantification is not possible, it appears that this polymer may be up to a few percents by weight of the total organic material.     

Sensory allometry, foraging task specialization and resource exploitation in honeybees

Insect societies are important models for evolutionary biology and sociobiology. The complexity of some eusocial insect societies appears to arise from self-organized task allocation and group cohesion. One of the best-supported models explaining self-organized task allocation in social insects is the response threshold model, which predicts specialization due to inter-individual variability in sensitivity to task-associated stimuli. The model explains foraging task specialization among honeybee workers, but the factors underlying the differences in individual sensitivity remain elusive. Here, we propose that in honeybees, sensory sensitivity correlates with individual differences in the number of sensory structures, as it does in solitary species. Examining European and Africanized honeybees, we introduce and test the hypothesis that body size and/or sensory allometry is associated with foraging task preferences and resource exploitation. We focus on common morphological measures and on the size and number of structures associated with olfactory sensitivity. We show that the number of olfactory sensilla is greater in pollen and water foragers, which are known to exhibit higher sensory sensitivity, compared to nectar foragers. These differences are independent of the distribution of size within a colony. Our data also suggest that body mass and number of olfactory sensilla correlate with the concentration of nectar gathered by workers, and with the size of pollen loads they carry. We conclude that sensory allometry, but not necessarily body size, is associated with resource exploitation in honeybees and that the differences in number of sensilla may underlie the observed differences in sensitivity between bees specialized on water, pollen and nectar collection.     

A novel enzyme-linked immunosorbent assay system for the quantitative analysis of Carassius auratus vitellogenin

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed to quantitatively detect Carassius auratus vitellogenin (VTG) levels. The protein levels in fish plasma are useful aquatic biomarkers of estrogenic compounds. This procedure involved an ELISA using monoclonal antibodies of CVmA2 and CVmA7 against Carassius auratus VTG, and CVmA7 conjugated to horseradish peroxidase as the detection antibody. The assay range was between 1 and 401.5 ng/ml and the recovery of the VTG added to Carassius auratus plasma was 92.5-109%. An in vitro assay was performed to measure low levels of the VTG, using primary hepatocytes of Carassius auratus induced by 17-beta estradiol (E2). The detection limit was 1 ng/ml and 137 ng/ml at the maximum. Within each sex of wild Carassius auratus, VTG levels from the river next to sewage treatment works (STWs) were much higher than those from the feeding stream. The Carassius auratus VTG bioassay could be a sensitive and useful tool for quantification of estrogenic principles in aquatic environments.     

Isolation and fractionation of soil humin using alkaline urea and dimethylsulphoxide plus sulphuric acid

Humin, the most recalcitrant and abundant organic fraction of soils and of sediments, is a significant contributor to the stable carbon pool in soils and is important for the global carbon budget. It has significant resistance to transformations by microorganisms. Based on the classical operational definition, humin can include any humic-type substance that is not soluble in water at any pH. We demonstrate in this study how sequential exhaustive extractions with 0.1 M sodium hydroxide (NaOH) + 6 M urea, followed by dimethylsulphoxide (DMSO) + 6% (v/v) sulphuric acid (H₂SO₄) solvent systems, can extract 70–80% of the residual materials remaining after prior exhaustive extractions in neutral and aqueous basic media. Solid-state ¹³C NMR spectra have shown that the components isolated in the base + urea system were compositionally similar to the humic and fulvic acid fractions isolated at pH 12.6 in the aqueous media. The NMR spectra indicated that the major components isolated in the DMSO + H₂SO₄ medium had aliphatic hydrocarbon associated with carboxyl functionalities and with lesser amounts of carbohydrate and peptide and minor amounts of lignin-derived components. The major components will have significant contributions from long-chain fatty acids, waxes, to cuticular materials. The isolates in the DMSO + H₂SO₄ medium were compositionally similar to the organic components that resisted solvation and remained associated with the soil clays. It is concluded that the base + urea system released humic and fulvic acids held by hydrogen bonding or by entrapment within the humin matrix. The recalcitrant humin materials extracted in DMSO + H₂SO₄ are largely biological molecules (from plants and the soil microbial population) that are likely to be protected from degradation by their hydrophobic moieties and by sorption on the soil clays. Thus, the major components of humin do not satisfy the classical definitions for humic substances which emphasise that these arise from microbial or chemical transformations in soils of the components of organic debris.