Consensus building during nest-site selection in honey bee swarms: the expiration of dissent

This study addresses a question that lies at the heart of understanding how the scouts in a honey bee swarm achieve unanimity in their dances, and so reach agreement in their choice of a future nest site: what causes the scouts that perform dances for the non-chosen sites to stop dancing for these sites? One possibility is that a scout stops dancing for a non-chosen site only after she follows a lively dance for another site, such as the site that is ultimately chosen. This hypothesis is contradicted by the finding that 23 out of 27 scouts (in 6 swarms) that danced initially for a non-chosen site stopped their dancing before they followed a dance for another site. Evidently, a scout that supports initially one of the non-chosen sites is likely to withdraw her support for this site even before she learns about another site. What causes her to do so? Close examination of the behavior of scouts revealed that they reduce the strength of their dancing (waggle runs/return to the swarm) for a given site over consecutive returns to the swarm. On average, the pattern of this reduction in dancing is strikingly linear, which suggests that it arises from an internal, neurophysiological process that automatically drives down a scout's motivation to dance for a site. Other results suggest that scouts from inferior sites start their dancing less strongly, and so cease their dancing more rapidly, than do scouts from superior sites. If so, then during the consensus-building process of the scouts, it is the support (the dancing) for inferior sites that is most likely to die out while it is the support for a superior site that is most likely to prevail.     

Intraspecific brood parasitism as a conditional reproductive tactic in the treehopper<Emphasis Type="Italic"> Publilia concava</Emphasis>

In species exhibiting egg guarding as well as communal egg laying, females may adopt the strategy of laying eggs in the nests of conspecifics and leaving without providing care (termed intraspecific brood parasitism). This study is the first to describe such a behavior in the insect Publilia concava (Hemiptera: Membracidae) through field studies that followed 849 marked females across 1,828 brood associations. While brood parasitism increased the total number of eggs in a host brood, it did not reduce the overall hatching success of host broods. Solitary females exhibited a range of guarding durations while parasitic females rarely remained to guard eggs. Females exhibiting the parasitic tactic increased their lifetime number of clutches without decreasing the number of solitary clutches that they were able to initiate. Estimates of egg number for these individual broods revealed that females adopting the parasitic tactic (in addition to solitary breeding) had higher lifetime fecundity relative to females that did not parasitize. In females that exhibited both tactics (solitary and parasitic), the parasitic tactic yielded a higher rate of oviposition. The major component of oviposition rate was the time to find hosts and this time decreased with increasing host availability across 222 replicate groups. Females exhibited a shift toward the parasitic tactic when host broods were more abundant (i.e. in larger groups and later in the season). However, the time to find hosts increased as the frequency of the parasitic tactic increased, suggesting that this tactic may be maintained through negative frequency dependence. The results of this study suggest that brood parasitism may be the preferred tactic, as part of a conditional strategy, when hosts are readily available with solitary breeding being the preferred tactic when hosts are in short supply.     

Courting virtual rivals increase ejaculate size in sticklebacks (<Emphasis Type="Italic">Gasterosteus aculeatus</Emphasis>)

Sperm competition occurs when sperm of two or more males compete to fertilize a given set of eggs. Game-theory models of sperm competition predict that males, which face an increased risk of sperm competition, will invest more sperm in a mating. In the pair-spawning three-spined stickleback ( Gasterosteus aculeatus), males attempt to steal fertilizations (i.e. to sneak) when they are in the courting phase, but not when they are parental. The behaviour of neighbouring males may therefore indicate the risk of sperm competition. We visually confronted males before spawning with computer animations of the same virtual stickleback showing two different behaviours: either courting (high risk of sperm competition) or brood-caring (low risk of sperm competition). We show that males invest significantly more sperm (absolutely and relatively with respect to available sperm in the testes) after the courting stimulus. The relative investment ratio between the courting and the brood-caring treatment was on average 1.75. Three-spined sticklebacks thus seem to assess the risk of sperm competition by the behaviour of neighbours and to adjust their ejaculate accordingly. This result suggests that the evolutionary force of sperm competition has led to precise mechanisms of future risk assessment.     

Innate antipredator responses of Arctic charr (<Emphasis Type="Italic">Salvelinus alpinus</Emphasis>) depend on predator species and their diet

The ability to discriminate between more dangerous and less dangerous predators can have serious fitness advantages for fish juveniles. This is especially true for hatchery-reared fish young used for stocking, because their post-release mortality is often much higher than that of wild-born conspecifics. We tested whether two coexisting fish predators and their different diets induce innate behavioral responses in predator-naive Arctic charr (Salvelinus alpinus) young originating from an endangered hatchery-bred population used for re-introductions. We predicted the antipredator responses of charr to be stronger towards chemical cues of brown trout (Salmo trutta) and pikeperch (Stizostedion lucioperca) than towards odorless control water. More pronounced antipredator behavior was predicted in treatments with predators fed on charr than when their diet consisted of another sympatric salmonid, European grayling (Thymallus thymallus), or when they were food-deprived. The Arctic charr young showed strong antipredator responses in all brown trout treatments, whereas odors of the less likely predator pikeperch were avoided with conspecific diet only. Freezing was the most sensitive antipredator behavior, as it was completely absent in control treatments. We found considerable individual variation in the amount and strength of antipredator responses. Although almost half of the charr failed to show antipredator behavior towards the piscivores, those with the innate ability showed highly sensitive recognition of predator odors. Our results indicate that the innate antipredator behavior of the juvenile fish is already finely tuned to respond specifically to chemical cues from different fish predators and even their diets.Communicated by J. Krause     

A technology for the treatment of olive-mill waste water in a continuously fed plant

A bench-scale pilot plant has been developed for the treatment of olive-mill water. The plant is fed in a continuous mode and implements a primary treatment (sand filtering and irradiation with UV-rays) and a secondary treatment (anoxic and aerobic biological treatment). Satisfactory results were obtained and the treated green water falls under the limits of the Italian legislation. Veratric and ferulic acid biodegradation were studied under anoxic conditions in order to verify the requirements for optimal biodegradation. Electronic Publication     

INFLUENCING WATER LEGISLATWE DEVELOPMENT WHAT TO DO AND WHAT TO AVOID1

ABSTRACT: Whomever would affect water policy development must influence the water legislative process. This paper discusses in the context of the Model State Water Allocation Code the following ten ideas about doing that: (1) seek the right timing for enactment; (2) set modest goals; (3) prepare a well-drafted proposal; (4) recognize diversity by offering legislative choices; (5) consider the impacts of adoption; (6) make alliances; (7) respect executive influence; (8) become involved in legislative interaction; (9) be flexible; and (10) persist. Heeding these ideas, although not guaranteeing enactment, is sure to be educational for participants and useful to legislators.     

Using community relations to reduce costs during remediation projects

Almost everyone who has been involved in a site remediation project has seen schedules slip and costs escalate due to political pressure from the public or the press. While focusing on remediation technologies and containment techniques to control costs, many organizations have neglected a major cost driver—public opinion. This article examines community relations from the perspective of an organization trying to control costs during a site remediation project. It details the strong correlation between the cost of a site cleanup and the level of public dissatisfaction and provides an organization with specific strategies on how to use proven communications techniques to lower costs. Examination of several case studies is provided, including a study involving a site in which community representatives actively worked to reduce project costs. It is clear that any responsible cleanup must be protective of public health and the environment. But it is becoming increasingly apparent that wise allocation of available resources has a profound effect on the program's ability to ensure public and environmental safety. In many cases, it has been proven that some costly cleanups—for example, involving excavation—sometimes actually increase risk by creating an exposure pathway where none existed before. In turn, such cleanups waste resources that are needed elsewhere. The challenge in dealing with this complicated issue is to help stakeholders understand the true ramifications of the choices that are faced at each site. If these stakeholders feel uninformed, powerless, or excluded from the process, it is likely that they will be unable to enter a productive discussion. The community relations programs outlined in documents such as a Superfund guidance can be helpful in familiarizing the community with site-related issues and with gathering public input. These activities act as a baseline for the programs discussed in this article. However, existing programs are not focused on providing a strategic advantage in reaching cleanup solutions and balancing health and environmental considerations with economic considerations.     

Evaluation of a fluorometric method for measuring low concentrations of ammonia in ambient air

A fluorometric method developed for measuring low concentrations of ammonium in marine and freshwater ecosystems was adapted for the analysis of ammonia in ambient air. The modified method entails collection of samples on an acid-treated solid adsorbent followed by analysis using a fluorometer. Optimal results were obtained using a commercially available sorbent tube containing 100 mg of acid-treated silica gel for sample collection, and an analytical protocol consisting of sample desorption in DI water, addition of orthopthaldialdehyde (OPA) working reagent, and room temperature incubation. Method accuracy and precision were evaluated by comparing experimentally determined quantities of ammonia to expected levels for sample loadings ranging from 0.16 [micro sign]g to 550 [micro sign]g-accuracy was generally within +/-20%. The estimated LOQ for the method is 0.08 [micro sign]g ammonia per sample which represents a 25-375-fold improvement in sensitivity compared to current NIOSH and OSHA methods for the measurement of ammonia in ambient air. The new method should be useful for applications requiring measurement of low concentrations of ammonia using personal sampling equipment or in the characterization of short-term fluctuations of ammonia concentrations in air.     

The residential lakeshore access allocation problem: Minimizing barrier effects on shoreline habitat buffers

Residential lakeshore development is causing increasing pressure on the lacustrine environment. Traditional landscaping patterns produce lakes ringed with lawns at the expense of the naturally occurring shoreline habitat. In this work, we present a mathematical model to select path locations to minimize impacts on the lakeshore environment, while providing convenient access to the landowners.     

Connectivity in priority area selection for conservation

The spatial relations of sites within networks of priority areas for conservation is critical to the long-term maintenance of key genetic, population and ecosystem processes. However, these relations have received relatively little attention in the development of mathematical methods for objectively identifying such networks. Here we present a novel heuristic for incorporating connectivity explicitly as part of the model constraints, provide an integer linear programming formulation for the same problem, describe an integer cutting procedure which defines a sequence of non-decreasing lower bounds on the optimal solution and report the results of some computational experiments using these algorithms.