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131.
Bernd Holger Briese Bernhard Schmidt Dieter Jendrossek 《Journal of Polymers and the Environment》1994,2(2):75-87
Four polyhydroxyalkanoate (PHA) depolymerases were purified from the culture fluid ofPseudomonas lemoignei: poly(3-hydroxybutyrate) (PHB), depolymerase A (M
r
, 55,000), and PHB depolymerase B (M
r
, 67,000) were specific for PHB and copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) as substrates. The third depolymerase additionally hydrolyzed poly(3-hydroxyvalerate) (PHV) at high rates (PHV depolymerase;M
r
, 54,000). The N-terminal amino acid sequences of the three purified proteins, of a fourth partially purified depolymerase (PHB depolymerase C), and of the PHB depolymerases ofComamonas sp. were determined. Four PHA depolymerase genes ofP. lemoignei (phaZ1,phaZ2,phaZ3, andphaZ4) have been cloned inEscherichia coli, and the nucleotide sequence ofphaZ1 has been determined recently (D. Jendrossek, B. Müller, and H. G. Schlegel,Eur. J. Biochem.
218, 701–710, 1993). In this study the nucleotide sequences ofphaZ2 andphaZ3 were determined.PhaZ1,phaZ2, andphaZ4 were identified to encode PHB depolymerase C, PHB depolymerase B, and PHV depolymerase, respectively.PhaZ3 coded for a novel PHB depolymerase ofP. lemoignei, named PHB depolymerase D. None of the four genes harbored the PHB depolymerase A gene, which is predicted to be encoded by a fifth depolymerase gene ofP. lemoignei (phaZ5) and which has not been cloned yet. The deduced amino acid sequences ofphaZ1–phaZ3 revealed high homologies to each other (68–72%) and medium homologies to the PHB depolymerase gene ofAlcaligenes faecalis T1 (25–34%). Typical leader peptide amino acid sequences, lipase consensus sequences (Gly-Xaa-Ser-Xaa-Gly), and unusually high proportions of threonine near the C terminus were found in PhaZ1, PhaZ2, and PhaZ3. Considering the biochemical data of the purified proteins and the amino acid sequences, PHA depolymerases ofP. lemoignei are most probably serine hydrolases containing a catalytical triad of Asp, His, and Ser similar to that of lipases. A comparison of biochemical and genetic data of various eubacterial and one eukaryotic PHA depolymerases is provided also.Paper presented at the Bio/Environmentally Degradable Polymer Society—Second National Meeting, August 19–21, 1993, Chicago, Illinois. 相似文献
132.
133.
134.
Ziyang Lou Bernd Bilitewski Nanwen Zhu Xiaoli Chai Bing Li Youcai Zhao Peter Otieno 《环境科学学报(英文版)》2015
As one of the largest human activities, World Expo is an important source of anthropogenic Greenhouse Gas emission (GHG), and the GHG emission and other environmental impacts of the Expo Shanghai 2010, where around 59,397 tons of waste was generated during 184 Expo running days, were assessed by life cycle assessment (LCA). Two scenarios, i.e., the actual and expected figures of the waste sector, were assessed and compared, and 124.01 kg CO2-equivalent (CO2-eq.), 4.43 kg SO2-eq., 4.88 kg NO3−-eq., and 3509 m3 water per ton tourist waste were found to be released in terms of global warming (GW), acidification (AC), nutrient enrichment (NE) and spoiled groundwater resources (SGWR), respectively. The total GHG emission was around 3499 ton CO2-eq. from the waste sector in Expo Park, among which 86.47% was generated during the waste landfilling at the rate of 107.24 kg CO2-eq., and CH4, CO and other hydrocarbons (HC) were the main contributors. If the waste sorting process had been implemented according to the plan scenario, around 497 ton CO2-eq. savings could have been attained. Unlike municipal solid waste, with more organic matter content, an incineration plant is more suitable for tourist waste disposal due to its high heating value, from the GHG reduction perspective. 相似文献
135.
Bernd Klauer Johannes Schiller Frauke Bathe 《Journal of Environmental Planning and Management》2015,58(11):1944-1960
The EU Water Framework Directive calls for cost effective measures to achieve a “good status” in all European ground, surface and coastal waters. Besides eutrophication, the degradation of hydromorphology is the main reason for failing this objective. In this paper, we conceptualise the interactive decision support process BASINFORM-M for finding proper locations for river restoration. The concept combines the recently proposed “stepping stone approach” from aquatic ecology with elements from cost effectiveness analysis, multi-criteria analysis and participatory approaches. BASINFORM-M exemplifies a shift away from the isolated restoration of single river reaches towards a consideration of functional relationships within river networks. 相似文献
136.
Fathi Zereini Friso Dirksen Bernd Skerstupp Hans Urban 《Environmental science and pollution research international》1998,5(4):223-230
Soil samples from the area of Hanau (Hessen, Germany) were analyzed for anthropogenic platinum-group elements (PGE). The results confirm the existence of two different sources for anthropogenic PGE: 1. automotive catalysts, and 2. PGE-processing plants. Both sources emit qualitatively and quantitatively different PGE spectra and PGE interelemental ratios (especially the Pt/Rh ratio). Elevated PGE values which are due to automotive catalysts are restricted to a narrow-range along roadside soil, whereas those due to PGE-processing plants display a large-area dispersion. The emitted PGE-containing particles in the case of automotive catalysts are subject to transport by wind and water, whereas those from PGE-processing plants are preferably transported by wind. This points to a different aerodynamic particle size. Pt, Pd, and Rh concentrations along motorways are dependent on the amount of traffic and the driving characteristics. 相似文献
137.
John Munthe Eva Brorström-Lundén Magnus Rahmberg Leo Posthuma Rolf Altenburger Werner Brack Dirk Bunke Guy Engelen Bernd Manfred Gawlik Jos van Gils David López Herráez Tomas Rydberg Jaroslav Slobodnik Annemarie van Wezel 《Environmental Sciences Europe》2017,29(1):13
Background
This paper describes a conceptual framework for solutions-focused management of chemical contaminants built on novel and systematic approaches for identifying, quantifying and reducing risks of these substances.Methods
The conceptual framework was developed in interaction with stakeholders representing relevant authorities and organisations responsible for managing environmental quality of water bodies. Stakeholder needs were compiled via a survey and dialogue. The content of the conceptual framework was thereafter developed with inputs from relevant scientific disciplines.Results
The conceptual framework consists of four access points: Chemicals, Environment, Abatement and Society, representing different aspects and approaches to engaging in the issue of chemical contamination of surface waters. It widens the scope for assessment and management of chemicals in comparison to a traditional (mostly) perchemical risk assessment approaches by including abatement- and societal approaches as optional solutions. The solution-focused approach implies an identification of abatement- and policy options upfront in the risk assessment process. The conceptual framework was designed for use in current and future chemical pollution assessments for the aquatic environment, including the specific challenges encountered in prioritising individual chemicals and mixtures, and is applicable for the development of approaches for safe chemical management in a broader sense. The four access points of the conceptual framework are interlinked by four key topics representing the main scientific challenges that need to be addressed, i.e.: identifying and prioritising hazardous chemicals at different scales; selecting relevant and efficient abatement options; providing regulatory support for chemicals management; predicting and prioritising future chemical risks. The conceptual framework aligns current challenges in the safe production and use of chemicals. The current state of knowledge and implementation of these challenges is described.Conclusions
The use of the conceptual framework, and addressing the challenges, is intended to support: (1) forwarding sustainable use of chemicals, (2) identification of pollutants of priority concern for cost-effective management, (3) the selection of optimal abatement options and (4) the development and use of optimised legal and policy instruments.138.
Sebastian Strunz Bernd Klauer Irene Ring Johannes Schiller 《Sustainability Science》2017,12(3):421-432
The flaws of mainstream economic methodology are becoming widely acknowledged. Should we, therefore, reject all of its concepts within the quest for sustainability? A predicament looms: neither would it make sense to neglect useful tools, nor to redundantly replicate the mainstream’s narrow perspective on sustainability problems. We argue that avoiding both fallacies is possible because power of judgment facilitates non-dogmatic methodological decisions: the scientists’ judgment, that is, the capacity to apply general concepts to specific situations, supports their decisions concerning which methods are suitable for tackling a given sustainability problem. The intersubjective quality of judgment prevents the resulting methodological pluralism from drifting toward arbitrariness. 相似文献
139.
Denise?HertwigEmail author Gopal?Patnaik Bernd?Leitl 《Environmental Fluid Mechanics》2017,17(3):551-578
Time-dependent three-dimensional numerical simulations such as large-eddy simulation (LES) play an important role in fundamental research and practical applications in meteorology and wind engineering. Whether these simulations provide a sufficiently accurate picture of the time-dependent structure of the flow, however, is often not determined in enough detail. We propose an application-specific validation procedure for LES that focuses on the time dependent nature of mechanically induced shear-layer turbulence to derive information about strengths and limitations of the model. The validation procedure is tested for LES of turbulent flow in a complex city, for which reference data from wind-tunnel experiments are available. An initial comparison of mean flow statistics and frequency distributions was presented in part I. Part II focuses on comparing eddy statistics and flow structures. Analyses of integral time scales and auto-spectral energy densities show that the tested LES reproduces the temporal characteristics of energy-dominant and flux-carrying eddies accurately. Quadrant analysis of the vertical turbulent momentum flux reveals strong similarities between instantaneous ejection-sweep patterns in the LES and the laboratory flow, also showing comparable occurrence statistics of rare but strong flux events. A further comparison of wavelet-coefficient frequency distributions and associated high-order statistics reveals a strong agreement of location-dependent intermittency patterns induced by resolved eddies in the energy-production range. The validation concept enables wide-ranging conclusions to be drawn about the skill of turbulence-resolving simulations than the traditional approach of comparing only mean flow and turbulence statistics. Based on the accuracy levels determined, it can be stated that the tested LES is sufficiently accurate for its purpose of generating realistic urban wind fields that can be used to drive simpler dispersion models. 相似文献
140.
Schröter-Kermani C Kreft D Schilling B Herrchen M Wagner G 《Journal of environmental monitoring : JEM》2006,8(8):806-811
In the framework of the German environmental specimen bank one-year old spruce shoots (Picea abies) and pine shoots (Pinus sylvestris) serve as bioindicators for the atmospheric pollution. Sampling is performed in two urbanized areas in western and eastern Germany (Warndt and Duebener Heide, respectively), and in seven different rural locations. Prior to archiving conifer shoots are continuously analyzed for a set of 17 individual polycyclic aromatic hydrocarbons (PAHs). The results from the two urbanized areas show that the atmospheric contamination with PAH has declined by about 75% between 1985 and 2004 at Warndt and by about 85% between 1991 and 2004 at Duebener Heide. However, summation operatorPAH concentrations stayed virtually constant at both locations since the end of the 1990s at levels of about 100 ng g(-1) wet weight (ww). In spruce shoots from rural areas current concentrations of PAHs are significantly lower and vary between 8 and 61 ng g(-1) ww. In all shoot samples the four low molecular aromatics phenanthrene, fluoranthene, pyrene, and chrysene dominate the pattern by contributing 60 to 90% to summation operatorPAH. The group of high molecular weight aromatics is dominated by benzo[b,j,k]fluoranthene, especially in spruce shoots originating from greater altitudes remarkable amounts of six and seven ringed PAHs could be detected. Despite the strong decrease of PAH concentrations in urban areas patterns of aromatics remained nearly unchanged in the observation period 1985 to 2004. 相似文献