Prenatal diagnosis of neural tube by measurement of serum alpha-fetoprotein defects in havana city

Prenatal screening was carried out in Havana City, Cuba, as part of a National Medical Genetics Programme, in order to detect elevated alpha-fetoprotein concentration in maternal serum (MS-AFP). A total of 97 900 pregnant women between 15 and 19 weeks of pregnancy were tested from 1982 to 1985. A double-antibody-sandwich technique was used. 173 malformed fetuses were detected. Confirmation was by ultrasonography, elevated AFP values in a second serum sample and in amniotic fluid and acetylcholinesterase. No termination of a normal pregnancy occurred. The screening of all pregnancies is easy, economical and useful for detecting not only fetuses suffering from open Neural Tube Defects (NTDs) and other severe disorders but also pregnancies at risk of further complications.     

Human Settlements on the Northern Shores of Lake Aral and Water Level Changes

The shore of Lake Aral in Kazakhstan is a perfect area for studying the human adaptation strategy to past climate changes. New archaeological material, gathered along the northern shores during the expedition of the INTAS project CLIMAN, is briefly presented. Changes in settlement activity during the Atlantic and Subboreal are related to lake level changes of the Aral Sea. A previ-ously proposed lake level maximum needs to be revised. In particular the lake level stand at 72/73 m, with an assumed age of 5000 BP is definitely refused. Based on the presented data the maximum lake level most probably never reached beyond 57/58 mean average sea level (masl). Furthermore the regression during the 15^th–16^th centuries has been underestimated. It may have been lower than the present day level. Thus the present desiccation of the Aral Sea is historically not unique, as a similar regression, probably induced by man as well, has occurred at least once during history. A readjustment of the water level is, therefore, possible at any time.     

Residual benefits of promiscuous soybean to maize (Zea mays L.) grown on farmers’ fields around Minna in the southern Guinea savanna zone of Nigeria

Biological nitrogen fixation (BNF) by promiscuous cultivars of soybeans (Glycine max (L.) Merr.) in cereal-based cropping systems of Nigeria’s moist savanna zone offers a potential for minimizing the investment made by resource-poor farmers on nitrogen fertilizers. A 3-year trial was conducted on five farmers’ fields in the southern Guinea savanna zone of Nigeria to assess the residual effects of two successive crops of promiscuous soybean cultivars on the yield of a following maize (Zea mays L.) crop. The soybean cultivars, TGX1456-2E (medium maturity) and TGX1660-19F (late maturity), were grown in 1996 and 1997. Treatments, imposed only in the first year of the trial, were: (i) uninoculated, (ii) inoculated with a mixture of two Bradyrhizobium strains, and (iii) fertilized with 60 kg N ha⁻¹. A fourth treatment was a plot left to fallow. In 1998, all the previous soybean and fallow plots were sown to maize without any fertilizer application. Results in 1996 and 1997 showed a soybean response to inoculation in the first year, but differences due to the residual effect of inoculation in the second year were not significant. Both cultivars showed a similar response to inoculation but responses at the five sites were varied. Soybean cultivar 1456-2E fixed 43–52% of its N amounting to 56–70 kg N ha⁻¹ and cultivar 1660-19F derived 39–54% of its N from N₂-fixation which amounted to 51–78 kg N ha⁻¹. Both cultivars had a high N harvest index resulting in a net removal of 52–95 kg N ha⁻¹ when both grain and stover were exported. Even when the stover was returned, there was a depletion of 23–65 kg N ha⁻¹, with 1456-2E removing more N than 1660-19F. Arbuscular-mycorrhizal infection on maize roots was 11–27% and dependent on previous soybean treatments and farmers’ fields. Plant height, shoot biomass, grain yield, and N uptake of maize were significantly greater in plots previously sown to soybean than in the fallow plots. In general, plots sown to the late maturing cultivar 1660-19F exhibited better residual effect, producing larger yield parameters than the plots planted with medium maturing 1456-2E.     

低浓度SO2长期暴露下菜豆的生长发育和气孔反应

本研究采用连续搅拌槽反应器系统(CSTR)对菜豆(Phaseolus vulgaris)进行低浓度SO_2长期暴露试验.结果表明,SO_2在植物可见伤害阈值以下抑制了莱豆生长,叶片气孔阻力随SO_2浓度的增加而增加.植物一旦脱离SO_2污染的环境,气孔就恢复到正常水平.切除子叶的菜豆生长明显受抑,但对SO_2敏感性和留子叶菜豆相同.     

Tectonic evolution of the Aravalli orogen (NW India): an inverted Proterozoic rift basin?

The Aravalli mountain range (AMR) in the northwestern part of the Indian Peninsula consists of two main Proterozoic metasedimentary and metaigneous sequences, the Aravalli and Delhi Supergroups, respectively, which rest over the Archaean gneissic basement. A synthesis and reinterpretation of the available geological, geochronological and geophysical data, including results of own field work and geophysical interpretations pertaining to the AMR, indicate its origin as an inverted basin: rifting into granitoid basement began ca. 2.5; Ga ago with Aravalli passive rifting (ca. 2.5–2.0 Ga) and Delhi active rifting (ca. 1.9–1.6 Ga). Associated mafic igneous rocks show both continental and oceanic tholeiitic geochemistry and are comparable with Phanerozoic, rift-related magmatic products. Available data showed no conclusive evidence for oceanic lithoshere and island-arc/active margin magmatic activity in the AMR. Subsequent inversion and orogeny (Delhi orogeny, ca. 1.5-1.4 Ga) lead to complex deformation and metamorphism. Only in the western and central zones has the basement been involved in this mid-Proterozoic (Delhi) deformation, whereas it is unaffected in the eastern part, except for local shear zones mainly along the basement/cover interface. The grade of metamorphism increases from the greenschist facies in the east to the amphibolite facies in the west with local HP assemblages. These latter are explained by rapid burial and exhumation of thin and cool continental lithosphere. Subsequently, during a final, mild phase of inversion, the Vindhyan basins consisting mainly of sandstones, limestones and shales, flanking the AMR formed which are comparable to foreland basins. The tectonic evolution of the AMR is therefore interpreted as an example of a major inverted continental rift and of a Proterozoic intra-continental orogen.     

Where Should Nature Reserves Be Located in South Africa? A Snake's Perspective

The present dispersion of nature reserves in South Africa is the historical result of a series of ad hoc decisions and may not be biologically optimal We have adopted a method to identify the optimal geography of nature reserves for the conservation of South Africa's snake fauna. The method of reserve selection operated on two tiers, and the spatial unit of analysis was a quarter-degree-square cell (∼625 km²). First, two scoring indices were used to rank cells with respect to species richness or to rarity. Second, two different iterative reserve-selection algorithms selected sets of cells (reserves), where each set represented all snake species at least once. Finally, the selected cells were examined for their present level of protection and their ranked scores. Depending on the algorithm chosen, only 23 or 29 cells were required to represent all species at least once; 72–78% of these cells already contained some level of protection; 59–70% of cells fell in areas of high species richness; and 72–91% of cells fell in areas with high rarity scores. Thus we conclude that most of the snake species in South Africa may be adequately protected with only modest acquisition of new reserves, and that the iterative algorithms appear to be efficient at siting cells in areas of high richness and rarity. We recommend that the reserve placement method outlined in this report be applied to as many other taxa as possible in the formulation of a national plan for an optimal reserve system for South Africa.     

Ecotypic differentiation of Laminaria longicruris in relation to seawater nitrate concentration

The ultraplankton (cell diameters >3 μm), which compromises about 70% of the biomass of phytoplankton in subtropical surface waters near Oahu, Hawaii, was isolated for growth rate studies. The specific growth rate (μ) was estimated from the rate of increase of the chlorophyll biomass during incubations in the absence of grazers. This growth rate of the ultraplankton ranged from 0.037 to 0.071 h^?1 (=1.3 to 2.5 doublings d^?1) during a period when P:B ratios of 5 to 14.5 μg C μg^?1 chl a h^?1 prevailed. The co-occurrence of atypically high P:B ratios and nonlimiting ambient nutrient concentrations suggests that the calculated values are higher than those characteristic of such subtropical ecosystems in general. Rates of ammonium uptake and photosynthesis by the >3 μm fraction were also compared to those of larger fractions. Organisms in the >3 μm fraction assimilated NH ₄ ⁺ at a rate which was about 75% greater than that of the 3 to 20 μm size fraction. Comparison of μ and P:B data collected over a 2 mo period (November–December, 1980) shows that the correlation between these two rate indices is nonlinear. The predominance of small-celled phytoplankton in oligotrophic waters is explained, in part, by its higher μ, its higher nutrient assimilation rates, and the absence of its loss through sedimentation.     

Patterns of the electric organ discharge during courtship and spawning in the mormyrid fish,Pollimyrus isidori

Summary Pollimyrus isidori's electric organ discharge (EOD) is of the pulse type. Patterns of EOD intervals were investigated prior to, during and following spawning behaviors as related with overt behaviors, and with the sound production by the nestbuilding male. Prior to the time of reproduction, isolated and socially interacting fish (n=15) showed characteristic discharge interval patterns for resting, swimming, probing, hovering and hiding activities. Males (n=8) and females (n=6) did not differ in their mean EOD repetition rates during resting (11.6±2.5 Hz), nor Short Bursts/min (less than 20 intervals of 8–13 ms). In interacting fish Long Bursts (greater than 20 intervals of 8–13 ms, lasting for more than 300 ms) were observed only during the attack and bite sequence. A pursuing fish displayed a rapid alternation of Long Bursts with Discharge Breaks (300–1000 ms silence) during the chase behavior. Avoidance behavior which followed from several attacks was correlated with a Medium Uniform Rate (8–12 Hz) normally lasting for 20 to 60 s, or a Discharge Arrest (silence greater than 1 s) in the submissive fish. The nocturnal courtship behavior began soon after dark (1900 h). Spawning typically started 2 to 5 h after dark, continuing for 2 to 6 h until about 0200 h. During courtship and spawning the female's brief visits (15–25 s) to the male's territory recurred every 30–60 s. At all other times the female was aggressively excluded from the nest region. Courtship and spawning behaviors are described along with the electrical displays identified from 19 spawnings in three fish pairs (from a total of 37 spawnings in 4 males and 4 females). Just prior to the onset of courtship behavior, with male territorial aggression beginning to decline, females switched from a Medium Sporadic Rate pattern (resting and hiding patterns; 13 Hz) to a Medium Uniform Rate pattern (6–8 Hz) while still in their hiding area. Females continued to display this uniform rate throughout the courship and spawning period, including the courtship and spawning bouts when Discharge Breaks or Arrests also occurred. This persistance distinguishes the courtship pattern from the similar avoidance pattern (see above). The male courtship and spawning EOD pattern was similar to the female's and unique for a territorial male. He switched from a High Sporadic Rate (swimming EOD pattern; about 18 Hz) to a regularized Medium Uniform Rate (about 9 to 11 Hz) only during courtship and spawning bouts, including 1–3 EOD Breaks during Vent-to-Vent coupling (average interval: 272±71 ms, n=37). No sooner had the female left the spawning site than he resumed displaying a High Sporadic Rate. This temporal correlation of reproductive behaviors with electrical displays suggests their instrumental role in mutual acceptance of mates. Males showed their sex-specific type of EOD phase-locking, the Preferred Latency Response, only during the first few hours of entry of a fish in their tank. Two females with EOD waveform features more typical of males also spawned repeatedly; waveform does not appear to be critical. Males stopped their nocturnal sound production for the later part of courtship and the whole spawning period. Except for infrequent attacks on the female between spawning bouts, the male did not resume singing until the end of spawning when all eggs were shed (around 0200 h); from this time on the male sang until dawn. The sequencing of the three acoustic elements (moans, grunts, growls) are described. A catalogue of discharge patterns correlated with overt behaviors (Tables 1, 2), and an integrated summary time table of P. isidori's complex reproductive behavior are presented.