Study of Graft Copolymerization of Soy Protein-Methyl Methacrylate: Preparation and Characterization of Grafted Films

Hybrid materials represent the family of compounds comprising mixtures of natural and synthetic materials. The study of this field is in a marked expansion since their preparation represents a valid methodology for optimizing the insufficient properties exhibited by materials integrally formed from naturally-occurring sources. In the present work, we describe soy protein modification by grafting reaction with methyl methacrylate. The reaction was confirmed by Fourier Transform Infrared Analysis and Carbon Nuclear Magnetic Resonance. In addition, films were prepared with the material by heat compression. Films were physically and mechanically characterized by determining contact angle with water, total soluble matter, moisture content, swelling in water, water vapor permeability, tensile strength, elongation at break and Young’s modulus. These measurements suggest that the material increased its hydrophobic character as compared with that of the control film since marked reductions in water vapor permeability, swelling and water solubility were determined. Moreover, their mechanical properties were improved by obtaining a more rigid material. These results represent an interesting advance in the preparation of hybrid biocomposites.     

Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo,Mexico

The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater has been reported as a result of fecal shedding of infected individuals. In this study, the occurrence of SARS-CoV-2 RNA was explored in primary-treated wastewater from two municipal wastewater treatment plants in Quintana Roo, Mexico, along with groundwater from sinkholes, a household well, and submarine groundwater discharges. Physicochemical variables were obtained in situ, and coliphage densities were determined. Three virus concentration methods based on adsorption-elution and sequential filtration were used followed by RNA isolation. Quantification of SARS-CoV-2 was done by RT-qPCR using the CDC 2020 assay, 2019-nCoV_N1 and 2019-nCoV_N2. The Pepper mild mottle virus, one of the most abundant RNA viruses in wastewater was quantified by RT-qPCR and compared to SARS-CoV-2 concentrations. The use of three combined virus concentration methods together with two qPCR assays allowed the detection of SARS-CoV-2 RNA in 58% of the wastewater samples analyzed, whereas none of the groundwater samples were positive for SARS-CoV-2 RNA. Concentrations of SARS-CoV-2 in wastewater were from 1.8 × 10³ to 7.5 × 10³ genome copies per liter (GC l⁻¹), using the N1 RT-qPCR assay, and from 2.4 × 10² to 5.9 × 10³ GC l⁻¹ using the N2 RT-qPCR assay. Based on PMMoV prevalence detected in all wastewater and groundwater samples tested, the three viral concentration methods used could be successfully applied for SARS-CoV-2 RNA detection in further studies. This study represents the first detection of SARS-CoV-2 RNA in wastewater in southeast Mexico and provides a baseline for developing a wastewater-based epidemiology approach in the area.

     

Classification of coastal waters according to the new Italian water legislation and comparison with the European Water Directive

New legislation for the protection of inland surface waters, transitional waters, coastal waters and ground waters has recently been established in Italy. This law presents a new integrated approach, where all water bodies are considered as complex ecosystems to be studied in each of their components. The new concept of environmental quality of the water body, based on the ecological and chemical status, is also introduced. At the same time (i.e. the end of 2000), the European Community approved the European Water Framework Directive based on the same basic environmental concepts and criteria of the new Italian law. This paper analyses the important points and innovations required by the new Italian legislation for monitoring and classification of marine coastal waters. Details of definitions, parameters, analysis and monitoring programs are discussed. A comparison with the European Water Framework Directive is eventually given, underlining the specific characteristics of the Mediterranean sea, which have to be taken into consideration when applying the European Directive to this particular ecoregion.     

Census Study of Real-Life Near-Side Crashes with Modern Side Airbag-Equipped Vehicles in the United States

Objective: This study aimed to investigate the crash characteristics, injury distribution, and injury mechanisms for Maximum Abbreviated Injury Score (MAIS) 2+ injured belted, near-side occupants in airbag-equipped modern vehicles. Furthermore, differences in injury distribution for senior occupants compared to non-senior occupants was investigated, as well as whether the near-side occupant injury risk to the head and thorax increases or decreases with a neighboring occupant.

Method: National Automotive Sampling System's Crashworthiness Data System (NASS-CDS) data from 2000 to 2012 were searched for all side impacts (GAD L&R, all principal direction of force) for belted occupants in modern vehicles (model year > 1999). Rollovers were excluded, and only front seat occupants over the age of 10 were included. Twelve thousand three hundred fifty-four MAIS 2+ injured occupants seated adjacent to the intruding structure (near-side) and protected by at least one deployed side airbag were studied. To evaluate the injury risk influenced by the neighboring occupant, odds ratio with an induced exposure approach was used.

Result: The most typical crash occurred either at an intersection or in a left turn where the striking vehicle impacted the target vehicle at a 60 to 70° angle, resulting in a moderate change of velocity (delta-V) and intrusion at the B-pillar. The head, thorax, and pelvis were the most frequent body regions with rib fracture the most frequent specific injury. A majority of the head injuries included brain injuries without skull fracture, and non-senior rather than senior occupants had a higher frequency of head injuries on the whole. In approximately 50% of the cases there was a neighboring occupant influencing injury outcome.

Conclusion: Compared to non-senior occupants, the senior occupants sustained a considerably higher rate of thoracic and pelvis injuries, which should be addressed by improved thorax side airbag protection. The influence on near-side occupant injury risk by the neighboring occupant should also be further evaluated. Furthermore, side airbag performance and injury assessments in intersection crashes, especially those involving senior occupants in lower severities, should be further investigated and side impact dummy biofidelity and injury criteria must be determined for these crash scenarios.     

Quantification and composition analysis of plastic pollution in riverine beaches of the lower Paraná River,Argentina

Environmental Science and Pollution Research - Plastic pollution and the numerous consequences it has on aquatic life have become a huge concern in recent years. While many studies have been...     

Occurrence of <Emphasis Type="Italic">Pepper Mild Mottle Virus</Emphasis> (PMMoV) in Groundwater from a Karst Aquifer System in the Yucatan Peninsula,Mexico

The Yucatan Peninsula of Mexico hosts a karst aquifer system that is the only source of freshwater for the area; however, it is vulnerable to human-mediated contamination. Pepper mild mottle virus (PMMoV) is one of the most abundant RNA viruses associated with human feces, making it a viable indicator for tracking fecal pollution in aquatic environments, including groundwater. In this study, groundwater samples collected from a karst aquifer from fresh and brackish water locations were analyzed for fecal indicator bacteria, somatic and male F+ specific coliphages, and PMMoV during the rainy and dry seasons. Total coliform bacteria were detected at all sites, whereas Escherichia coli were found at relatively low levels <40 MPN/100 ml. The highest average concentrations of somatic and male F+ specific coliphages were 920 and 330 plaque forming units per 100 ml, respectively, detected in freshwater during the rainy season. PMMoV RNA was detected in 85% of the samples with gene sequences sharing 99–100% of nucleotide identity with PMMoV sequences available in GenBank. Quantification of PMMoV genome copies (GC) by quantitative real-time PCR indicated concentrations ranging from 1.7 × 10¹ to 1.0 × 10⁴ GC/L, with the highest number of GC detected during the rainy season. No significant correlation was observed between PMMoV occurrence by season or water type (p > 0.05). Physicochemical and indicator bacteria were not correlated with PMMoV concentrations. The abundance and prevalence of PMMoV in the karst aquifer may reflect its environmental persistence and its potential as a fecal indicator in this karst aquifer system.     

Hygienic quality of faeces treated in urine diverting vermicomposting toilets

On-site sanitation solutions have gained much interest in recent years. One such solution is the urine diverting vermicomposting toilet (UDVT). This study evaluated the hygienic quality of the composted material in six UDVTs in operation in France. Samples were taken from three sampling positions in each toilet, with increasing distance from the fresh material. The concentration of Salmonella spp., Enterococcus spp., thermotolarent coliforms and coliphages were analysed and plotted against a number of variables. The variables found to have the greatest impact was the pH (for Enterococcus spp. and thermotolarent coliforms (TTC)) and time since last maintenance (coliphages). The pH was found to correlate with the material maturity. The current practise of maintenance can cause recontamination of the stabilised material and increase the risk of regrowth of pathogenic microorganisms. A modification in the maintenance procedure, in which a fourth maturation point is introduced, would eliminate this risk. UDVTs were found to be a good on-site sanitation option as the maintenance requirement is small and the system effectively reduced odour and concentration of pathogen and indicator organisms in human waste while keeping the accumulation of material down to a minimum. If the vermicompost is to be used for crops consumed raw, an additional sanitisation step is recommended.     

Influence of the pesticides glyphosate,chlorpyrifos and atrazine on growth parameters of nonochratoxigenic Aspergillus section Nigri strains isolated from agricultural soils

This investigation was undertake to determine the effect of glyphosate, chlorpyrifos and atrazine on the lag phase and growth rate of nonochratoxigenic A. niger aggregate strains growing on soil extract medium at ?0.70, ?2.78 and ?7.06 MPa. Under certain conditions, the glyphosate concentrations used significantly increased micelial growth as compared to control. An increase of about 30% was observed for strain AN 251 using 5 and 20 mg L^?1 of glyphosate at ?2.78 MPa. The strains behaved differently in the presence of the insecticide chlorpyrifos. A significant decrease in growth rate, compared to control, was observed for all strains except AN 251 at ?2.78 MPa with 5 mg L^?1. This strain showed a significant increase in growth rate. With regard to atrazine, significant differences were observed only under some conditions compared to control. An increase in growth rate was observed for strain AN 251 at ?2.78 MPa with 5 and 10 mg L^?1 of atrazine. By comparison, a reduction of 25% in growth rate was observed at ?7.06 MPa and higher atrazine concentrations. This study shows that glyphosate, chlorpyrifos and atrazine affect the growth parameters of nonochratoxigenic A. niger aggregate strains under in vitro conditions.