Effects of smelter sulfur dioxide emissions: a spatiotemporal perspective using carbon isotopes in tree rings

We wanted to test the hypothesis that forest exposure to phytotoxic gases indirectly affects their carbon uptake. We estimated that the reduction of photosynthesis may have reached 20 to 30% at a site located 9 km (test site) from the Horne copper smelter in Rouyn-Noranda, which is a point source of SO2. Twenty-one spruce trees older than 100 yr were selected from seven sites at various distances from the smelter to evaluate conditions prior to and during the periods of smelter operation. The carbon isotope results obtained from spruce tree rings at our test site reveal an unprecedented and abrupt shift of +4/1000 after the onset of smelter operations. This large and permanent shift exceeds natural variations in regional pre-smelter series or in the series at a remote control site. All trees up to 116 km downwind from the smelter show delta13C positive shifts following the onset of operations. There is also a clear inverse relationship between the amplitude of the first-order trends and distance from the smelter. Those delta13C trends indicate that trees exposed to high levels of SO2 decrease their level of CO2 uptake through activation of stomatal closure. This is strongly supported by the significant departure of the Rouyn-Noranda trends from those measured for trees from non-industrialized areas of the Northern Hemisphere, or calculated using global atmospheric conditions. Considering the large number of SO2 point sources in North America, our results imply that CO2 uptake by the boreal forest in the vicinity of these sources may be lower than previously thought.     

Epilithic Diatoms in an Alpine Lake (Traunsee,Austria) Affected by Soda and Salt Mining Industries

Traunsee, a 191 m deep Alpine lake in Austria, is affected by industrial tailings from the soda and salt mining industries since 1883. In 1998 littoral water chloride concentrations ranged between 40 and 85 mg L^-1 and the highest conductivity was 560 S cm^-1, which is almost double as high as the values reported from the two nearby reference lakes. Chloride concentrations increased towards the location of the industrial salt and soda emission into the lake. Analogously to the chloride gradient, the epilithic littoral diatom flora changes towards the waste inlet. Shifts in the species percentages towards the emission source, a high percentage of taxa with large conductivity tolerances, the presence of a small Achnanthes minutissima Kützing morphotype, and occurrences of taxa focused at habitats of higher electrolyte content, indicate subtle impacts on the epilithic diatom flora. An analysis of the seasonal succession of the epilithic diatoms at the waste inlet compared to a lake intern reference site, reveals that only during the late summer period in 1998 the diatom assemblage at the waste inlet became significantly different, indicating seasonally restricted effects of the industrial emissions.     

Atrazine, isoproturon, mecoprop, 2,4-D, and bentazone adsorption onto iron oxides

Iron oxides are important components influencing the adsorption of various inorganic and organic compounds in soils and sediments. In this study the adsorption on iron oxides of nonionic and ionic pesticides was determined as a function of solution pH, ionic strength, and pesticide concentration. The investigated iron oxides included two-line ferrihydrite, goethite, and lepidocrocite. Selected pesticides comprised atrazine (6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine), isoproturon [3-(4-isopropylphenyl)-1,1-dimethylurea)], mecoprop [(RS)-2-(4-chloro-2-methylphenoxy)propionic acid], 2,4-D (2,4-dichlorophenoxyacetic acid), and bentazone [3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide]. The adsorption of the nonionic pesticides (atrazine and isoproturon) was insignificant, whereas the adsorption of the acidic pesticides (mecoprop, 2,4-D, and bentazone) was significant on all investigated iron oxides. The adsorption capacity increased with decreasing pH, with maximum adsorption reached close to the pKa values. The addition of CaCl2 in concentrations from 0.0025 to 0.01 M caused the adsorption capacity to diminish. The adsorption of bentazone was significantly lower than the adsorption of mecoprop and 2,4-D, illustrating the importance of a carboxyl group in the pesticide structure. The adsorption capacity on the iron oxides increased in the order: lepidocrocite < goethite < two-line ferrihydrite. The maximum adsorption capacities of meco-prop and 2,4-D on goethite were found to be equivalent to the site density of singly coordinated hydroxyl groups on the faces of the dominant (110) form, suggesting that singly coordinated hydroxyl groups are responsible for adsorption. Differences in adsorption capacities between iron oxides can be explained by differences in the surface site density of singly coordinated hydroxyl groups. The maximum measured adsorption capacity of mecoprop on two-line ferrihydrite was equivalent to 0.2 mol/mol Fe.     

Impacts of interannual climate variability on past and future forest composition

Numerous analyses of the possible impacts of future climatic changes on tree species composition have been published for both lowland and high-elevation forests. Most of these studies were based on the application of forest "gap" models, and the vast majority of them considered only changes in the average of climatic parameters over time. In this study, we use a unique data set on reconstructed past climatic variations to analyse forest dynamics simulated by the forest gap model ForClim. This analysis forms the basis for a systematic exploration of the ecological effects of changing means vs. changing variability of climate on central European forests. A reconstruction of historical climate covering the last 470 years in the Swiss lowlands (ClimIndex) is extrapolated to a transect across the alpine (cold) treeline and used to simulate the influence of climate variations on the time scale of decades on forest biomass and tree species composition at both sites. While the simulation at the low-elevation site shows little sensitivity to climate variations, the results from upper subalpine forests suggest that two major dieback events would have occurred at elevations above the current but below the climatic tree line, induced by clusters of exceptionally cold summers. The results are in agreement with available dendrochronological data and with documentary evidence on massive negative impacts on flora and fauna at high elevations during these periods. We conclude that ForClim is capable of capturing the effects on tree population dynamics of climate variability at these sites as reconstructed from the ClimIndex record. A factorial design is used to address the sensitivity of ForClim to changes of the long-term averages vs. changes of the variability of monthly temperature and precipitation data. To this end, the simulated tree species composition of near-natural forests is examined along a climate gradient in Europe. The results indicate that there are three types of forest response: (1) little sensitivity to both kinds of change, (2) strong sensitivity to changes in the means, but little sensitivity to changing variability, and (3) strong sensitivity to changing variability at least in parts of the examined climate space. Half of the cases investigated fall under the third category, suggesting that emphasis should be placed on also assessing the sensitivity of ecosystems to future changes in climate variability rather than on changes of average values alone. Electronic Publication     

Compostability of Poly(lactide): Degradation in an Inert Solid Medium

Commercial poly(lactide) degradation was studied in an inert solid medium simulating compost conditions, with the aim to achieve a complete carbon balance of the polymer degradation. The mineralisation rate at the end of the test was compared to those obtained for poly(lactide) degradation in compost. It was shown that the mineralisation rate after 45 days of degradation was quite lower in inert solid medium than in compost but the standard deviation of data was enhanced. A protocol for both extraction and quantification of the carbon included in the different degradation by-products was proposed and the carbon balance of the polymer degradation was followed during the test with a satisfactory accuracy. The non-degraded PLA material was recovered during the test, hence the evolution of the glass transition temperature and the molecular weight was followed. A two-step degradation mechanism was highlighted in inert solid medium, showing the fundamental role of abiotic reactions for PLA degradation in compost.     

University of Benin water supply system: Microbiological and physico-chemical assessments

The microbiological and physico-chemical characteristics of the drinking water supplied by the Central Borehole at the University of Benin, Ugbowo Campus were investigated. The investigation entailed assessment of the pH, turbidity, total suspended solids, total dissolved solids, dissolved oxygen, temperature, salinity, conductivity, nitrate, nitrite, phosphate, sulphate, chloride, N-nitroso compounds, cadmium, chromium, nickel, lead, zinc, manganese, iron, coliform count, BOD₅ and COD of the water at the Central Borehole and at ten residential quarters. The assessment indicated that the water was fit for drinking and other domestic applications. Results were also compared with WHO, EU and Nigeria FEPA standards. The results showed that the pH values of the water (5.01–5.86) and total coliform count (1–2/100 ml) expressed as MPN were outside the limits set by the WHO, EU and FEPA. The data also showed that the other water quality parameters assessed were within WHO, EU and FEPA permissible limits. The results of ANOVA showed that significant changes occurred during distribution.     

Integration of noninvasive prenatal prediction of fetal blood group into clinical prenatal care

Incompatibility of red blood cell blood group antigens between a pregnant woman and her fetus can cause maternal immunization and, consequently, hemolytic disease of the fetus and newborn. Noninvasive prenatal testing of cell-free fetal DNA can be used to assess the risk of hemolytic disease of the fetus and newborn to fetuses of immunized women. Prediction of the fetal RhD type has been very successful and is now integrated into clinical practice to assist in the management of the pregnancies of RhD immunized women. In addition, noninvasive prediction of the fetal RhD type can be applied to guide targeted prenatal prophylaxis, thus avoiding unnecessary exposure to anti-D in pregnant women. The analytical aspect of noninvasive fetal RHD typing is very robust and accurate, and its routine utilization has demonstrated high sensitivities for fetal RHD detection. A high compliance with administering anti-D is essential for obtaining a clinical effect. Noninvasive fetal typing of RHC/c, RHE/e, and KEL may become more widely used in the future. © 2014 John Wiley & Sons, Ltd.