Background, aim, and scope In this work, the potential for using olive-mill solid waste as an organic amendment for biochemical and biological restoration
of a trichloroethylene-contaminated soil, which has previously been stabilized through vermicomposting processes, has been
explored.
Materials and methods Trichloroethylene-contaminated water was pumped into soil columns with a layer of vermicompost at 10-cm depth (biobarrier
system). The impacts of the trichloroethylene on the microbial community were evaluated by determining: (1) the overall microbial
activity (estimated as dehydrogenase activity) and enzyme activities related to the main nutrient cycles (β-glucosidase, o-diphenoloxidase, phosphatase, urease, and arylsulphatase activities). In addition, isoelectric focusing of the soil extracellular
humic-β-glucosidase complexes was performed to study the enzymatically active humic matter related to the soil carbon cycle.
(2) The soil bacterial diversity and the molecular mechanisms for the bacterial resistance to organic solvents were also determined.
For this, polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) was used to detect changes in bacterial
community structure and PCR-single-strand conformational polymorphism (SSCP) was developed and optimised for detection and
discrimination of the resistance-nodulation-division (RND) genes amplified from the contaminated soils.
Results Vermicompost reduced, with respect to the unamended soil, about 30% of the trichloroethylene leaching during the first month
of the experiment. Trichloroethylene had a marked negative effect on soil dehydrogenase, β-glucosidase, urease, phosphatase,
and arylsulphatase activities. Nevertheless, the vermicompost tended to avoid this toxic effect. Vermicompost also displays
stable humic-β-glucosidase complexes that increased the extracellular activity related to C-cycle in the contaminated soils.
The isoelectric focusing technique showed a more biochemically active humic matter in the soil sampled under the vermicompost.
The behaviour of the three main phyla of bacteria isolated from the DGGE bands was quite different. Bands corresponding to
Actinobacteria disappeared, whereas those affiliated with Proteobacteria remained after the trichloroethylene contamination. The disappeared Actinobacteria became visible in the soil amended with the vermicompost. Bands corresponding to Bacteriodetes appeared only in columns of contaminated soils. In this study, six types of RND proteins were detected by PCR-SSCP in the
natural soil, three in the trichloroethylene-contaminated soil and 7/5 in trichloroethylene-contaminated soil above/below
the vermicompost in the biobarrier columns. Trichloroethylene tended to reduce or eliminate all the clones detected in the
uncontaminated soil, whereas new efflux pumps appeared in the biobarrier columns.
Discussion Although enzymes incorporated into the humic substances of vermicomposted olive wastes are quite stable, trichloroethylene
also inhibited the background levels of the soil extracellular β-glucosidase activity in the amended soils. The decrease was
less severe in the biobarrier system, but in any case, no relation was found between the levels of trichloroethylene in soil
and extracellular β-glucosidase activity, or between the latter and the quantity of humic carbon in soils. The isoelectric
focusing technique was carried out in the humic fraction to determine whether the loss of activity occurred in overall extracellular
β-glucosidase or in that linked to stable humic substances (humic–enzyme complexes). The contaminated soils showed the lower
enzyme activities, whereas contaminated and amended soils presented greater quantity of focalised (and therefore stable) humic
carbon and spectra heterogeneity: very different bands with higher enzyme activities. No clear relationship between trichloroethylene
concentration in soil and diversity of the bacterial population was noted. Similar patterns could be found when the community
structures of bacteria and microbial activity were considered. Since the use of the dehydrogenase assay has been recognised
as a useful indicator of the overall measure of the intensity of microbial metabolism, these results could be attributed to
PCR-DGGE methodology, since the method reveals the presence of dominant populations regardless of their metabolic state. Trichloroethylene
maintained or even increased the number of clones with the DNA encoding for RND proteins, except for the contaminated soil
located above the vermicompost. However, the main effect of trichloroethylene was to modify the structure of the community
in contaminated soils, considering the type of efflux pumps encoded by the DNA extracted from soil bacteria.
Conclusions Trichloroethylene inhibited specific functions in soil and had a clear influence on the structure of the autochthonous bacterial
community. The organic matter released by the vermicomposted olive waste tended to avoid the toxic effect of the contaminant.
Trichloroethylene also inhibited the background levels of the soil extracellular β-glucosidase activity, even when vermicompost
was present. In this case, the effect of the vermicompost was to provide and/or to stimulate the humic-β-glucosidase complexes
located in the soil humic fraction >104, increasing the resistance of the enzyme to the inhibition. The bacterial community from the soil presented significantly
different mechanisms to resistance to solvents (RND proteins) under trichloroethylene conditions. The effect of the vermicompost
was to induce these mechanisms in the autochthonous bacterial community and/or incorporated new bacterial species, able to
grow in a trichloroethylene-contaminated ambient. Coupled biochemical and molecular methodologies are therefore helpful approaches
in assessing the effect of an organic amendment on the biochemical and biological restoration of a trichloroethylene-contaminated
soil.
Recommendations and perspectives Since the main biochemical and biological effects of the organic amendment on the contaminated soil seem to be the incorporation
of biochemically active humic matter, as well as new bacterial species able to grow in a trichloroethylene-contaminated ambient,
isoelectric focusing and PCR-SSCP methodologies should be considered as parts of an integrated approach to determine the success
of a restoration scheme. 相似文献
Anthropogenic impacts on biodiversity can lead to biotic homogenization (BH) and biotic differentiation (BD). BH is a process of increasing similarity in community composition (including taxonomic, functional, and phylogenetic components), whereas BD is a process of decreasing similarity over space and time. Here, we conducted a systematic review of BH and BD in plant communities in tropical and subtropical forests to identify trends and knowledge gaps. Our bibliometric search in the Web of Science returned 1989 papers, of which 151 matched our criteria and were included in the analysis. The Neotropical region had the largest number of articles, and Brazil was the most represented country with 92 studies. Regarding the type of change, homogenization was more frequent than differentiation (noted in 69.6% of publications). The taxonomic diversity component was measured more often than functional and phylogenetic diversity components. Most studies (75.6%) assessed homogenization and differentiation based on a single observation in time; as opposed to few studies that monitored plant community over multiple years. Forest fragmentation was cited as the main determinant of homogenization and differentiation processes (57.2% of articles). Our results highlight the importance of evaluating community composition over time and more than taxonomic components (i.e., functional and phylogenetic) to advance understanding of homogenization and differentiation. Both processes were scale dependent and not mutually exclusive. As such, future research should consider differentiation as a potential transition phase to homogenization and that potential differences in both processes may depend on the spatial and temporal scale adopted. Understanding the complexity and causes of homogenization and differentiation is essential for biodiversity conservation in a world increasingly affected by anthropogenic disturbances. 相似文献
According to the desperado sibling hypothesis, chicks of obligately siblicidal species kill their junior broodmates as early as possible because junior broodmates face dire ecological prospects and are consequently predisposed to aggressively overthrow elder broodmates by all-out aggression. The agonistic behavior of junior chicks is virtually unknown because they die so young. We investigated the agonistic behavior of hypothetical desperados by fostering junior brown boobies (Sula leucogaster, an obligately siblicidal species) into nests of the blue-footed booby (S. nebouxii, a facultatively siblicidal species) containing an older singleton blue-foot chick. Controls were junior blue-footed boobies fostered into the same situation. Junior brown boobies were 7 times more aggressive than controls and most of them dominated their elder and larger nestmates. Four of nine brown booby juniors showed relentless aggression, delivering up to 711 pecks, bites and pushes (including "expulsion pushes") per hour, thereby overwhelming nestmates 90% heavier and permanently expelling one of them from the nest. Similarly, in natural broods of two surviving brown booby chicks, the losing chick was 13 times as aggressive as blue-foot subordinates, up to at least age 7 weeks. Contrast of the two species of booby suggests the evolution of agonistic roles within broods may be partly driven by selection on potential victims to express a level and type of aggressiveness appropriate to their status-related ecological prospects. 相似文献
Food and Environmental Virology - The experience of COVID19 pandemic has demonstrated the real concern of biological agents dispersed in the air and surfaces environments. Therefore, the need of a... 相似文献
Modified kaolin clays were used as adsorbents for SO(2) gas adsorptions. The clays were heated up to 900 °C previous to acid treatments with 0.5 N sulfuric acid solutions at boiling temperature during different times up to 1440 min. Equilibrium adsorption at 25 °C and 0.1 MPa was carried out by using a volumetric apparatus. The samples were characterized by chemical analysis, X-ray diffraction and infrared analysis. The heating of the clays followed by acid treatment improved the adsorption capacity of the kaolin clays. The presence of amorphous silica and hydroxyl in the final products improved SO(2) adsorption capacity. Better properties for SO(2) adsorption were found in kaolin rich in not well ordered kaolinite clay mineral. 相似文献
New finds of bones of the Egyptian Mongoose (Herpestes ichneumon), one from Portugal and one from Spain, were directly 14C dated to the first century AD. While the Portuguese specimen was found without connection to the Chalcolithic occupation of the Pedra Furada cave where it was recovered, the Spanish find, collected in the city of Mérida, comes from a ritual pit that also contained three human and 40 dog burials. The finds reported here show that the Egyptian mongoose, contrary to the traditional and predominant view, did not first arrive in the Iberian Peninsula during the Muslim occupation of Iberia. Instead, our findings are consistent with the hypothesis that the species was first introduced by the Romans, or at least sometime during the Roman occupation of Hispania. Therefore, radiocarbon dating of new archaeological finds of bones of the Egyptian Mongoose (Herpestes ichneumon) in the Iberian Peninsula push back the confirmed presence of the species in the region by approximately eight centuries, as the previously oldest dated record is from the ninth century. With these new dates, there are now a total of four 14C dated specimens of Egyptian mongooses from the Iberian Peninsula, and all of these dates fall within the last 2000 years. This offers support for the hypothesis that the presence of the species in Iberia is due to historical introductions and is at odds with a scenario of natural sweepstake dispersal across the Straits of Gibraltar in the Late Pleistocene (126,000–11,700 years ago), recently proposed based on genetic data.
Studies were undertaken to determine the adsorption behavior of α -cypermethrin [R)-α -cyano-3-phenoxybenzyl(1S)-cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate, and (S)-α-cyano-3-phenoxybenzyl (1R)-cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate] in solutions on granules of cork and activated carbon (GAC). The adsorption studies were carried out using a batch equilibrium technique. A gas chromatograph with an electron capture detector (GC-ECD) was used to analyze α -cypermethrin after solid phase extraction with C18 disks. Physical properties including real density, pore volume, surface area and pore diameter of cork were evaluated by mercury porosimetry. Characterization of cork particles showed variations thereby indicating the highly heterogeneous structure of the material. The average surface area of cork particles was lower than that of GAC. Kinetics adsorption studies allowed the determination of the equilibrium time—24 hours for both cork (1–2 mm and 3–4 mm) and GAC. For the studied α -cypermethrin concentration range, GAC revealed to be a better sorbent. However, adsorption parameters for equilibrium concentrations, obtained through the Langmuir and Freundlich models, showed that granulated cork 1–2 mm have the maximum amount of adsorbed α-cypermethrin (qm) (303 μg/g); followed by GAC (186 μ g/g) and cork 3-4 mm (136 μg/g). The standard deviation (SD) values, demonstrate that Freundlich model better describes the α -cypermethrin adsorption phenomena on GAC, while α -cypermethrin adsorption on cork (1-2 mm and 3-4 mm) is better described by the Langmuir. In view of the adsorption results obtained in this study it appears that granulated cork may be a better and a cheaper alternative to GAC for removing α -cypermethrin from water. 相似文献
Public participation within environmental monitoring may contribute to increasing the knowledge on the state of the environment at the same time it promotes citizens' involvement in environmental protection. However, the use of voluntary collected data is limited due to a lack of confidence in data collection procedures. Additionally, data quality is often unknown and the data are usually dispersed and non-structured. Information and communication technologies (ICT) may promote the use of voluntary collected data through the development of a collaborative system that incorporates tools and methodologies to facilitate data collection, access and validation. Furthermore, the use of ICT may promote public involvement within environmental monitoring, since it facilitates communication among all the stakeholders. This paper analyses the role of ICT in developing a system for environmental collaborative monitoring intending to promote the use of volunteer collected data. It starts by analysing the role of volunteers within environmental monitoring and continues analysing the potential of ICT to take advantage of the benefits of using data collected by citizens. A collaborative system that allows the public to express its knowledge on the state of the environment is described. Special emphasis is given to tools that explore non-traditional types of environmental data such as images, sounds and videos in association with spatial information. To illustrate the above mentioned concepts, a case study for beach quality monitoring developed within the Senses@Watch project, is described. 相似文献
The present work presents the results of 137Cs concentration in seawater, fish and sediments samples collect in 11 sampling points, crossing the Brazilian Southeastern coastal region, from Vitória (ES) to Santos (SP), on a routine basis from 1997 to 2002. This monitoring program was carried out by the Instituto de Radioproteção e Dosimetria (IRD/CNEN/MCT), in cooperation with the Instituto de Estudos do Mar Almirante Paulo Moreira (IEAPM/Brazilian Navy), aiming at determining artificial radionuclides in marine samples. Additionally to the 137Cs results, 90Sr concentrations in fish samples from 1998 to 2002 are also reported.The 137Cs in seawater follows a lognormal distribution, with a geometric mean of 1.8 Bq m-3 and a geometric standard deviation of 1.4 (n=54), decay corrected to June/2002. For 137Cs levels in fish samples a geometric mean of 0.19 Bq kg−1 and a geometric standard deviation of 2.9 (n=39), decay corrected to June/2002, with a range of 0.03 to 1.7 Bq kg−1, were obtained. Based on the 137Cs mean concentration in fish as well as in seawater, a transfer factor of 1 × 102 was calculated, which is quite in agreement with the recommended value found in the Safety Report Serie 19. 相似文献