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Soudek P Petrová S Benešová D Dvořáková M Vaněk T 《Journal of environmental radioactivity》2011,102(6):598-604
Hydroponicaly cultivated plants were grown on medium containing uranium. The appropriate concentrations of uranium for the experiments were selected on the basis of a standard ecotoxicity test. The most sensitive plant species was determined to be Lactuca sativa with an EC50 value about 0.1 mM. Cucumis sativa represented the most resistant plant to uranium (EC50 = 0.71 mM). Therefore, we used the uranium in a concentration range from 0.1 to 1 mM.Twenty different plant species were tested in hydroponic solution supplemented by 0.1 mM or 0.5 mM uranium concentration. The uranium accumulation of these plants varied from 0.16 mg/g DW to 0.011 mg/g DW. The highest uranium uptake was determined for Zea mays and the lowest for Arabidopsis thaliana. The amount of accumulated uranium was strongly influenced by uranium concentration in the cultivation medium. Autoradiography showed that uranium is mainly localized in the root system of the plants tested. Additional experiments demonstrated the possibility of influencing the uranium uptake from the cultivation medium by amendments. Tartaric acid was able to increase uranium uptake by Brassica oleracea and Sinapis alba up to 2.8 times or 1.9 times, respectively. Phosphate deficiency increased uranium uptake up to 4.5 times or 3.9 times, respectively, by Brassica oleracea and S. alba. In the case of deficiency of iron or presence of cadmium ions we did not find any increase in uranium accumulation. 相似文献
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Morrison L Baumann HA Stengel DB 《Environmental pollution (Barking, Essex : 1987)》2008,152(2):293-303
The relative abundance and variation of Cr, Co, Cd and Pb in Ascophyllum nodosum and intertidal surface sediments from six locations around the coast were assessed over six seasons. Higher Cd and Pb levels in Galway Docks and Cork Harbour were attributed to localised inputs of these metals from municipal and domestic waste, while at a reference site (Ballyconneely), high algal Cr concentrations were considered a function of geological setting rather than anthropogenic loading. Little seasonal variation was observed, with the exception of higher Co levels in plants in winter, associated with growth dynamics and increased fluvial inputs. In comparison with previously published data for metals in A. nodosum from the North Atlantic, with the exception of localised hot spots, the Irish coastline is still a relatively pristine environment. A. nodosum may be successfully and easily used as a biomonitor of metal contamination in coastal waters. 相似文献
14.
Mass spectrometry fingerprinting of humic acids extracted from different soils has been carried out using laser desorption/ionization mass spectrometry (LDI-TOF MS). LDI-TOF MS provides characteristic mass spectra fingerprints for the humic acids of different origin. The information given in the fingerprints was evaluated for natural grouping trends in the samples by neural networks computing tools, such as self-organizing feature map (SOFM). This approach is efficient for recognizing patterns in the humic acids samples independently of their characteristic variability; variability characterizing natural products such as humic substances. The use of multi-layer perceptron artificial neural networks gave a successful classification of the samples. 相似文献
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David Chitayat Robert W. Marion Linda Squillante Dagmar K. Kalousek Kiron M. Das MD Ph.D. 《黑龙江环境通报》1990,10(11):725-732
Since its introduction, prenatal diagnosis of chromosomal and metabolic disorder by mid-trimester amniocentesis has relied upon the use of a mixture of fetal cells obtained from amniotic fluid. Little knowledge has been gained in the sorting of these cells for diagnosis of tissue-specific disorders. In an attempt to determine the contribution of fetal colonic mucosal cells to the overall amniocyte population, we used the colonic epithelial-specific monoclonal antibody (MC-Ab) 7E12H12, IgM isotype. Specimens of the small intestine, colon, buccal mucosa, kidney, urinary bladder, and umbilical cord were obtained from electively aborted normal fetuses of 12–28 weeks' gestation. All of these specimens were examined with 7E12H12 by the immunoperoxidase technique. The MC-Ab reacted with the colonic epithelial cells but not with any of the other tissues. In addition, 40 amniotic fluid samples obtained from women between 16 and 18 weeks of gestation, who underwent amniocentesis because of advanced maternal age, were tested using a fluorescent activated cell sorter. Among the amniotic fluid specimens examined, 18·4 ± 10·3 percent cells reacted with 7E12H12. Double immunofluorescence studies revealed that all Mc-Ab-stained cells contained secretory component, confirming that they were epithelial in origin. All fetuses whose amniotic fluid was analysed had normal karyotypes and amniotic fluid alpha-fetoprctein levels that were also normal. This study demonstrates that cell-specific Mc-Ab can be used to detect colon cells in the amniotic fluid and that colon cells contribute significant numbers in the mixture of amniotic fluid cells. This technique could be helpful in the prenatal diagnosis of disorders in which the flow of amniotic fluid through the fetal intestine is impaired, such as cystic fibrosis, imperforate anus, Hirschsprung aganglionic megacolon, and intestinal atresia. 相似文献
18.
Wolfgang Cramer Uta Fritsch Rik Leemans Sabine Lütkemeier Dagmar Schröter Allan Watt 《Regional Environmental Change》2008,8(3):125-134
Biodiversity is essential for multiple aspects of human life and well-being, but many current assessments of the functioning
of biodiversity and ecosystems, understanding of risks posed by environmental change and the best practice of their management
of ecosystems are lacking a unified scientific and conceptual basis. Methods such as scenario analysis, and terms such as
ecosystem services, are widely used, but their meaning is understood in many different ways depending on context, user needs
and experience of researchers. In order to advance the conceptual basis for ecosystem analysis and management in a rapidly
changing world, as well as the ability of young scientists to reflect upon these concepts, we have organised five 2-week-long
summer schools in Peyresq, a remote village in the Southern French Alps. In total 173 participants have worked intensively
with 69 experienced researchers and a team of conveners and tutors in order to discuss a broad range of views on topics on
ecosystem analysis and functioning. Topics ranged from conditions of and threats to various ecosystems due to environmental
change, models and scenarios for assessment, stakeholder perceptions and needs for information, to the social and economic
contexts for biodiversity. We report our experience from these schools, present the training concept which has emerged from
them and suggest lines of further development.
相似文献
Wolfgang CramerEmail: |
19.
Ibrahim Macharia Dagmar Mithöfer Hermann Waibel 《Environment, Development and Sustainability》2013,15(4):887-902
Pesticide handling practices have a strong bearing on the exposure of toxic effects to target and nontarget organism. A clear understanding of determinants of pesticide handling practices is a precondition in the design and implementation of policy intervention. To accomplish this, a household survey of 425 respondents was conducted in 2008. Majority of the farmers (85 %) had inappropriately handled pesticides, mainly through, unsafe storage (23 %), unsafe disposal of leftover in either sprays solutions, or rinsate and empty pesticide containers (40 %), failure to wear the required minimum protective gear (68 %), or overdosed pesticides (27 %). However, majority of those farmers were aware of the risks of pesticide use, with over 81 % expressing the view that pesticides have harmful effects on human health, livestock, beneficial arthropods, and on water. Econometric models showed that pesticide handling practices were significantly influenced by variation in record keeping, main source advice on pesticide use, toxicity of pesticide, and geographical location. Pesticide risk perception and negative impacts experiences had no association with handling practices. The study recommends policymakers to design effective, participatory, and location targeted outreach programmes, which deal specifically on promotion of record keeping and reduction in use of harmful pesticides. 相似文献
20.
Thomas A. Jefferson Dagmar Fertl Jaime Bolaños-Jiménez Alexandre N. Zerbini 《Marine Biology》2009,156(6):1109-1124
Due to indications that misidentification (largely confusion among dolphins of the genera Delphinus and Stenella) in the past had led to erroneous assumptions of distribution of the two species of common dolphins (Delphinus delphis and D. capensis) in the western Atlantic Ocean, we conducted a critical re-examination of records of the genus Delphinus from this region. We compiled 460 ‘plottable’ records, required support for confirmation of genus and species identifications,
and found many records lacking (and some clearly misidentified). When we plotted only the valid records (n = 364), we found evidence of populations in only three areas, and apparent absence throughout much of the tropical/subtropical
regions. Off the east coast of the US and Canada, D. delphis is found from the Georgia/South Carolina border (32°N) north to about 47–50°N off Newfoundland. Since the 1960s, they have
apparently been absent from Florida waters. There is no evidence that dolphins of the genus occur in the Gulf of Mexico. Reports
of common dolphins from most of the Caribbean Basin are also rejected, and the only place in that region where they are confirmed
to occur is off central-eastern Venezuela (a coastal D. capensis population). Off eastern South America, common dolphins appear to be restricted to south of 20°S. There is a coastal long-beaked
population found in the South Brazil Bight, and one or more short-beaked populations south and offshore of this (ranging south
to at least northern Argentina). The results are very different from commonly-accepted patterns of distribution for the genus
in the Atlantic. Most areas of distribution coincide with moderate to strong upwelling and common dolphins appear to avoid
warm, tropical waters. This study shows that great care must be taken in identification of similar-appearing long-beaked delphinids,
and that uncritical acceptance of records at face value can lead to incorrect assumptions about the ranges of the species
involved. 相似文献