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51.
内分泌干扰物 (Endocrine Disrupting Compounds, EDCs)可以通过干扰下丘脑-垂体-性腺(HPG)轴来影响生殖系统。虽然目前已有筛选内分泌干扰物的体外检测方法,但这些方法在用于体内实验时却有着不稳定的准确性。本文记录了以黑头呆鱼(Pimephales promelas)的下丘脑-垂体-性腺轴与肝(HPG-L)的共培养组织作为组织外植体来模拟体内反应的结果。我们对成年鱼的大脑(下丘脑),垂体,性腺和肝进行了单独和共同培养的检测来确定可以在体内重复的情况与组合。只有共培养体表现出去甲雄三烯醇酮对于雌二醇,睾酮和卵黄生成素生成趋势的影响。较低的暴露剂量会抑制激素生成,而较高的暴露剂量则会促进激素生成,形成U型作用曲线。这些数据表明下丘脑-垂体-性腺-肝轴的全部组织的共同培养可以作为体内实验与体外实验的连接,从而预测在完整生物体内内分泌系统的扰乱。本实验中以组织为基础的下丘脑-垂体-性腺-肝系统作为一个灵活的体内系统的解构版本得到了更好的实验控制。通过分离、审查和重组需要的组织,我们能够检测到生物系统功能与对于内分泌干扰物反应中的微小变化。
精选自Theresa K. Johnston, Edward Perkins, Duncan C. Ferguson, Donald M. Cropek. Tissue explant co-culture model of the hypothalamic-pituitary-gonadal-liver axis of the fathead minnow (Pimephales promelas) as a predictive tool for endocrine disruption. Environmental Toxicology and Chemistry: Volume 35, Issue 10, pages 2530–2541, October 2016.
DOI: 10.1002/etc.3415
详情请见http://onlinelibrary.wiley.com/doi/10.1002/etc.3415/full 相似文献
52.
The International Union for Conservation of Nature's Red List of Threatened Species (RLS) is the key global tool for objective, repeatable assessment of species’ extinction risk status, and plays an essential role in tracking biodiversity loss and guiding conservation action. Satellite remote sensing (SRS) data sets on global ecosystem distributions and functioning show exciting potential for informing range-based RLS assessment, but their incorporation has been restricted by low temporal resolution and coverage of data sets, lack of incorporation of degradation-driven habitat loss, and noninclusion of assumptions related to identification of changing habitat distributions for taxa with varying habitat dependency and ecologies. For poorly known mangrove-associated Cuban hutias (Mesocapromys spp.), we tested the impact of possible assumptions regarding these issues on range-based RLS assessment outcomes. Specifically, we used annual (1985–2018) Landsat data and land-cover classification and habitat degradation analyses across different internal time series slices to simulate range-based RLS assessments for our case study taxa to explore potential assessment uncertainty arising from temporal SRS data set coverage, incorporating proxies of (change in) habitat quality, and assumptions on spatial scaling of habitat extent for RLS parameter generation. We found extensive variation in simulated species-specific range-based RLS assessments, and this variation was mostly associated with the time series over which parameters were estimated. However, results of some species-specific assessments differed by up to 3 categories (near threatened to critically endangered) within the same time series, due to the effects of incorporating habitat quality and the spatial scaling used in RLS parameter estimation. Our results showed that a one-size-fits-all approach to incorporating SRS information in RLS assessment is inappropriate, and we urge caution in conducting range-based assessments with SRS for species for which habitat dependence on specific ecosystem types is incompletely understood. We propose novel revisions to parameter spatial scaling guidelines to improve integration of existing time series data on ecosystem change into the RLS assessment process. 相似文献
53.
Many fundamental traits of species measured at different levels of biological organization appear to scale as a power law to body mass (M) with exponents that are multiples of 1/4. Recent work has united these relationships in a "metabolic theory of ecology" (MTE) that explains the pervasiveness of quarter-power scaling by its dependence on basal metabolic rate (B), which scales as M(0.75). Central to the MTE is theory linking the observed -0.25 scaling of maximum population growth rate (rm) and body mass to the 0.75 scaling of metabolic rate and body mass via relationships with age at first reproduction (alpha) derived from a general growth model and demographic theory. We used this theory to derive two further predictions: that age at first reproduction should scale inversely to mass-corrected basal metabolic rate alpha infinity (B/M)(-l) such that rm infinity (B/M)1. We then used phylogenetic generalized least squares and model selection methods to test the predicted scaling relationships using data from 1197 mammalian species. There was a strong phylogenetic signal in these data, highlighting the need to account for phylogeny in allometric studies. The 95% confidence intervals included, or almost included, the scaling exponent predicted by MTE for B infinity M(0.75), rm infinity M(-0.25), and rm infinity alpha(-1), but not for alpha infinity M(0.25) or the two predictions that we generated. Our results highlight a mismatch between theory and observation and imply that the observed -0.25 scaling of maximum population growth rate and body mass does not arise via the mechanism proposed in the MTE. 相似文献
54.
Isolated Spring Wetlands in the Great Basin and Mojave Deserts,USA: Potential Response of Vegetation to Groundwater Withdrawal 总被引:3,自引:0,他引:3
Desert springs, often the sole sources of water for wildlife and cattle, support wetland and wetland/upland transition ecosystems
including rare and endemic species. In the basin and range province in Nevada, USA, springs in the Great Basin and Mojave
deserts are sustained by interconnected deep carbonate and shallow basin-fill aquifers which are threatened by proposed groundwater
withdrawal to sustain rapidly expanding urban areas, a common problem in arid regions worldwide. This paper draws on historic
groundwater data, groundwater modeling, and studies of environmental controls of spring ecosystems to speculate on the potential
effects of groundwater withdrawal and water table decline on spring-supported vegetation. The focus is on springs in the Great
Basin and Mojave deserts representative of those that may be affected by future, planned groundwater withdrawal. Groundwater
withdrawal is expected to reduce spring discharge directly through reduced flows from the shallow basin-fill aquifer or through
reduction of the hydraulic head of the deep carbonate aquifer. This flow reduction will truncate the outflow stream, reducing
the areal cover of wetland and wetland/upland transition vegetation. Lowering the local water table may also reduce the amount
of upland phreatophytic vegetation by causing water levels to drop below plant rooting depths. Percolation of salts to surface
soils may be reduced, eventually altering desert shrub cover from halophytes to nonhalophytes. The extent of these effects
will vary among springs, based on their distance from extraction sites and location relative to regional groundwater flow
paths. On-site monitoring of biotic variables (including cover of selected hygrophytes and phreatophytes) should be a necessary
complement to the planned monitoring of local hydrologic conditions. 相似文献
55.
Jeffrey S. Duncan Martha T. Fernandez Patricia L. Abbitt Siva Thiagarajah Stuart S. Howards W. Allen Hogge M.D. 《黑龙江环境通报》1990,10(3):175-182
To delineate the natural history of fetal multicystic dysplastic kidneys (MDKs), all cases that were prenatally detected in the Prenatal Diagnosis Center of the University of Virginia from September 1985 to 31 August 1988 were reviewed. All patients were followed through the Center with serial ultrasound evaluations at approximately 4-week intervals, and each liveborn infant was evaluated and followed by one of the authors (S.S.H.) Of the 14 cases detected, ten were detected in the second trimester, the earliest at 16·5 weeks' gestation. Of the nine fetuses with non-lethal disease, there were two cases in which the lesion remained unchanged during observation. Both had an initial diagnosis in the third trimester. In those cases diagnosed in the second trimester (7), all showed an initial increase in the size and number of cysts, followed by involutional changes either in utero (2) or in the neonatal period (3). Two infants had immediate surgical removal of the MDK, one because of respiratory compromise, and the other because of an uncertain diagnosis on renal scan. Abnormalities of the contralateral kidney were found in 7 of 14 fetuses. Five were lethal conditions. Associated non-renal abnormalities were common in bilateral MDK (80 per cent), but rare in unilateral MDK (11 per cent). 相似文献
56.
ABSTRACTThe long-standing debate on environmental policy ‘leaders’ and ‘laggards’ lends itself to a new analysis following with the advent of the 2008 economic crisis. This paper, therefore, asks the question to what extent do European Union (EU) member states have the capacity and willingness to implement EU environmental policy amid austerity, budget cuts, and rising costs over the period 2008–2014. Building upon previous studies, 26 interviews with European, Greek and United Kingdom participants and records of environmental infringements, the paper provides a contemporary picture of the environmental policy ‘leader-laggard’ dynamic in Europe. The findings demonstrate that the impact of the economic crisis seems contestable and varies amongst member states, while the reduction in environmental infringements appears to have a link with the decrease in economic activity. Although environmental policy ‘leaders’ maintain patterns of strong implementation, the improved implementation performance of some ‘laggards’ reflects a shift in their implementation patterns amid a period of intense political and economic controversies. 相似文献
57.
John Duncan Middleton 《Local Environment》2003,8(2):155-165
Health is a basic human right. Improving health requires social and environmental justice and sustainable development. The 'health for all' movement embraces principles shared by other social movements--in sustainable development, community safety and new economics. These principles include equity, democracy, empowerment of individuals and communities, underpinned by supportive environmental, economic and educational measures and multi-agency partnerships. Health promotion is green promotion and inequality in health is due to social and economic inequality. This paper shows how health, environmental and economic sustainability are inextricably linked and how professionals of different disciplines can work together with the communities they serve to improve local health and quality of life. It gives examples of how local policy and programme development for public health improvement can fit in with global and national policy-making to promote health, environmental and social justice. 相似文献
58.
Aung M Khaing Swe K Oo T Kyaw Moe K Leimgruber P Allendorf T Duncan C Wemmer C 《Journal of environmental management》2004,72(4):205-216
We reconstructed the history of Chatthin Wildlife Sanctuary (CWS) to understand how social and economic events, and policy changes affected the sanctuary's condition. We surveyed 25 villages surrounding CWS to evaluate past and present ecological conditions, compare the results with historical accounts and identify causal relationships. During the first half of the 20th century, the primary threat was the government's reduction of old growth forest to supply fuel wood for the British-built railway. The railroad opened the area to colonization, but the villagers' impact on timber and wildlife was low. From 1945 to 1988, villagers became the primary force of landscape degradation. The post-war windfall of firearms increased hunting pressure, and populations of large mammal started to decline. With the economic decline of the 1970s and 1980s, the community's demand for game and forest products intensified, and the large mammal fauna was reduced from eleven to four species. From 1988-2003, the forests surrounding the sanctuary were fragmented and degraded. The absence of large predators rendered the park safe for livestock, and the combined effects of grazing and removal of forest products seriously degraded habitat within CWS. Major threats to CWS during the past two decades have resulted from land use decisions in which government-planned economic enterprises caused encroachment by villagers. Stabilization and recovery of this sanctuary will require management compatible with human needs, including expanded buffer zones, better core area protection, community forestry projects, and probably relocation of villages within the park. 相似文献
59.
Duncan Knowler 《Journal of Environmental Planning and Management》2005,48(5):747-770
Incorporating environmental and natural resource impacts into the benefit-cost analysis of projects in the developing world now relies on a solid body of theory and recommended techniques, but there remain concerns with how this is done in practice. One aspect arises when time and resource constraints make highly sophisticated analysis difficult or impossible. This paper discusses situations where analyses can be improved using relatively simple ‘short cut’ techniques that substitute for more formal analysis that cannot be undertaken realistically or provide an added perspective on project viability. Case studies are employed to illustrate the techniques, based upon the author's experience with appraisals at the World Bank, Food and Agriculture Organization (FAO) and the International Fund for Agricultural Development (IFAD). It is shown that short cut techniques can dramatically alter the outcome of an appraisal at relatively little cost. However, caution is advised since such techniques can be misused, so that the development of suitable protocols is desirable. 相似文献
60.
Implementation of a continuous-flow stirred bioreactor system in the bioremediation of heavy metals from industrial waste water 总被引:1,自引:0,他引:1
The industrial applicability of immobilized non-viable yeast biomass (Saccharomyces cerivisiae) in continuous-flow stirred bioreactors was tested with respect to metal removal from electroplating effluent solutions containing Cu, Cd, Cr, Ni and Zn in excess of the stipulated water quality criteria. Two systems, a dual bioreactor and triple bioreactor system in series, were tested and compared regarding their efficiency of metal removal. The system containing two bioreactors linked in series provided a more efficient solution to the problem. The introduction of a third bioreactor into the system had a marginal effect on the amount of metal removed. Subsequent to treatment within the bioreactors the levels of Cu, Cr and Ni in the effluent complied with the stipulated drinking water quality criteria and, although in excess of these criteria only 18% of the initial Zn and 17% of the Cd remained in solution. 相似文献