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941.
Sutton MA Nemitz E Erisman JW Beier C Bahl KB Cellier P de Vries W Cotrufo F Skiba U Di Marco C Jones S Laville P Soussana JF Loubet B Twigg M Famulari D Whitehead J Gallagher MW Neftel A Flechard CR Herrmann B Calanca PL Schjoerring JK Daemmgen U Horvath L Tang YS Emmett BA Tietema A Peñuelas J Kesik M Brueggemann N Pilegaard K Vesala T Campbell CL Olesen JE Dragosits U Theobald MR Levy P Mobbs DC Milne R Viovy N Vuichard N Smith JU Smith P Bergamaschi P Fowler D Reis S 《Environmental pollution (Barking, Essex : 1987)》2007,150(1):125-139
Recent research in nitrogen exchange with the atmosphere has separated research communities according to N form. The integrated perspective needed to quantify the net effect of N on greenhouse-gas balance is being addressed by the NitroEurope Integrated Project (NEU). Recent advances have depended on improved methodologies, while ongoing challenges include gas-aerosol interactions, organic nitrogen and N(2) fluxes. The NEU strategy applies a 3-tier Flux Network together with a Manipulation Network of global-change experiments, linked by common protocols to facilitate model application. Substantial progress has been made in modelling N fluxes, especially for N(2)O, NO and bi-directional NH(3) exchange. Landscape analysis represents an emerging challenge to address the spatial interactions between farms, fields, ecosystems, catchments and air dispersion/deposition. European up-scaling of N fluxes is highly uncertain and a key priority is for better data on agricultural practices. Finally, attention is needed to develop N flux verification procedures to assess compliance with international protocols. 相似文献
942.
Paz-Alberto AM Sigua GC Baui BG Prudente JA 《Environmental science and pollution research international》2007,14(7):498-504
Background, Aims and Scope The global problem concerning contamination of the environment as a consequence of human activities is increasing. Most of
the environmental contaminants are chemical by-products and heavy metals such as lead (Pb). Lead released into the environment
makes its way into the air, soil and water. Lead contributes to a variety of health effects such as decline in mental, cognitive
and physical health of the individual. An alternative way of reducing Pb concentration from the soil is through phytoremediation.
Phytoremediation is an alternative method that uses plants to clean up a contaminated area. The objectives of this study were:
(1) to determine the survival rate and vegetative characteristics of three grass species such as vetivergrass, cogongrass
and carabaograss grown in soils with different Pb levels; and (2) to determine and compare the ability of the three grass
species as potential phytoremediators in terms of Pb accumulation by plants.
Methods The three test plants: vetivergrass (Vetiveria zizanioides L.); cogongrass (Imperata cylindrica L.); and carabaograss (Paspalum conjugatum L.) were grown in individual plastic bags containing soils with 75 mg kg−1 (37.5 kg ha−1) and 150 mg kg−1 (75 kg ha−1) of Pb, respectively. The Pb contents of the test plants and the soil were analyzed before and after experimental treatments
using an atomic absorption spectrophotometer. This study was laid out following a 3 × 2 factorial experiment in a completely
randomized design.
Results On the vegetative characteristics of the test plants, vetivergrass registered the highest whole plant dry matter weight (33.85–39.39
Mg ha−1). Carabaograss had the lowest herbage mass production of 4.12 Mg ha−1 and 5.72 Mg ha−1 from soils added with 75 and 150 mg Pb kg−1, respectively. Vetivergrass also had the highest percent plant survival which meant it best tolerated the Pb contamination
in soils. Vetivergrass registered the highest rate of Pb absorption (10.16 ± 2.81 mg kg−1). This was followed by cogongrass (2.34 ± 0.52 mg kg−1) and carabaograss with a mean Pb level of 0.49 ± 0.56 mg kg−1. Levels of Pb among the three grasses (shoots + roots) did not vary significantly with the amount of Pb added (75 and 150
mg kg−1) to the soil.
Discussion Vetivergrass yielded the highest biomass; it also has the greatest amount of Pb absorbed (roots + shoots). This can be attributed
to the highly extensive root system of vetivergrass with the presence of an enormous amount of root hairs. Extensive root
system denotes more contact to nutrients in soils, therefore more likelihood of nutrient absorption and Pb uptake. The efficiency
of plants as phytoremediators could be correlated with the plants’ total biomass. This implies that the higher the biomass,
the greater the Pb uptake. Plants characteristically exhibit remarkable capacity to absorb what they need and exclude what
they do not need. Some plants utilize exclusion mechanisms, where there is a reduced uptake by the roots or a restricted transport
of the metals from root to shoots. Combination of high metal accumulation and high biomass production results in the most
metal removal from the soil.
Conclusions The present study indicated that vetivergrass possessed many beneficial characteristics to uptake Pb from contaminated soil.
It was the most tolerant and could grow in soil contaminated with high Pb concentration. Cogongrass and carabaograss are also
potential phytoremediators since they can absorb small amount of Pb in soils, although cogongrass is more tolerant to Pb-contaminated
soil compared with carabaograss. The important implication of our findings is that vetivergrass can be used for phytoextraction
on sites contaminated with high levels of heavy metals; particularly Pb.
Recommendations and Perspectives High levels of Pb in localized areas are still a concern especially in urban areas with high levels of traffic, near Pb smelters,
battery plants, or industrial facilities that burn fuel ending up in water and soils. The grasses used in the study, and particularly
vetivergrass, can be used to phytoremediate urban soil with various contaminations by planting these grasses in lawns and
public parks.
ESS-Submission Editor: Dr. Willie Peijnenburg (wjgm.peijnenburg@rivm.nl) 相似文献
943.
Heavy metals in untreated/treated urban effluent and sludge from a biological wastewater treatment plant 总被引:1,自引:0,他引:1
Oliveira Ada S Bocio A Trevilato TM Takayanagui AM Domingo JL Segura-Muñoz SI 《Environmental science and pollution research international》2007,14(7):483-489
Background, Aim and Scope
The presence of heavy metals in wastewater is one of the main causes of water and soil pollution. The aim of the present study was to investigate the removal of Cd, Cu, Pb, Hg, Mn, Cr and Zn in urban effluent by a biological wastewater treatment, as well as to quantify the levels of As, Be, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Sn, Tl, V and Zn in dewatering sludge from the Biological Wastewater Treatment Plant to Ribeirão Preto (RP-BWTP), Brazil.Materials and Methods
Concentrations of Cd, Cr, Cu, Mn and Pb in wastewater and those of Ni in sludge were determined by atomic absorption spectrophotometry with graphite furnace atomization. Mercury concentrations in wastewater were measured by hydride generation atomic spectrophotometry, and Zn levels were determined by atomic absorption spectrophotometry using acetylene flame. In sludge, the levels of As, Be, Cd, Cr, Cu, Fe, Hg, Mn, Pb, Sn, Tl, V and Zn were determined by inductively coupled plasma-mass spectrometry.Results
The percentages of removal efficiency (RE) were the following: Hg 61.5%, Cd 60.0%, Zn 44.9%, Cu 44.2%, PB 39.7%, Cr 16,5% and Mn 10.4%. In turn, the mean concentrations (mg/kg) of metals in dewatering sludge followed this increasing order: Tl (<0.03), Hg (0.31), Be (0.43), As (1.14), Cd (1.34), V (59.2), Pb (132.1), Sn (166.1), Cr (195.0), Mn (208.1), Ni (239.4), Cu (391.7), Zn (864.4) and Fe (20537).Discussion
The relationship between metal levels in untreated wastewater, as well as the removal efficiency are in agreement with previous data from various investigators, It is important to note that metal removal efficiency is not only affected by metal ion species and concentration, but also by other conditions such as operating parameters, physical, chemical, and biological factors.Conclusions
Metal values recorded for treated wastewater and sludge were within the maximum permitted levels established by the Environmental Sanitation Company (CETESB), São Paulo, Brazil.Recommendations
There is an urgent need for the authorities who are responsible for legislation on sludge uses in agriculture of establishing safety levels for As, Be, Hg, Sn, Tl and V.Perspectives
According to the current metal levels, RP-BWTP sludge might be used for agriculture purposes. However, for an environmentally safe use of sewage sludge, further studies including systematic monitoring are recommended. Annual metal concentrations and predicted variations of those elements in the sludge should be monitored.944.
García De Llasera MP Rodríguez-Castillo A Vera-Avila LE 《Journal of environmental science and health. Part. B》2007,42(6):615-627
The effect of organic matter on the solid-phase extraction (SPE) efficiency for pesticides belonging to different chemical groups (urea-derivatives, carbamates and triazines) and having different polarities, was simultaneously studied for the first time in pure and simulated water samples. SPE was carried out in precolumns packed with C18 silica or styrene-divinylbenzene copolymer PLRP-S phases on-line coupled to high performance liquid chromatography (HPLC) analysis. Retention factors in water (k'(W)) were estimated for 25 compounds and used for the calculation of the theoretical breakthrough volume (Vb(T)) in pure water. Experimental breakthrough volumes (Vb(E)) were first determined using purified and deionized water as the matrix for selected compounds having Vb(T) < 500 mL; then, the same water with an added humic acid sodium salt (HA) at 0.4-5.6 mg/L of dissolved organic carbon (DOC) content, was used as the matrix for compounds having VbE < 500 mL in pure water. Several polar pesticides showed negative linear or logarithmic Vb(E) curves depending on HA content; their recoveries were also determined in environmental samples having low dissolved organic carbon values, between 0.5-6.4 mg/L. A similar behavior was observed for these compounds in simulated and natural water samples, where DOC concentration and the percolated volume (Vp) mainly determine the solute recoveries values. However, the variation of recoveries as a function of DOC content could be negative or null depending on the two examined conditions (Vp lower or larger than Vb(E) in pure water). Results demonstrated that breakthrough volume must always be considered to correctly interpret the participation of dissolved humic material on the SPE efficiency of organic micropollutants in water. 相似文献
945.
Schröder P Scheer CE Diekmann F Stampfl A 《Environmental science and pollution research international》2007,14(2):114-122
Background, Aim and Scope
Numerous herbicides and xenobiotic organic pollutants are detoxified in plants to glutathione conjugates. Following this enzyme
catalyzed reaction, xenobiotic GS-conjugates are thought to be compartmentalized in the vacuole of plant cells. In the present
study, evidence is presented for long range transport of these conjugates in plants, rather than storage in the vacuole. To
our knowledge this is the first report about the unidirectional long range transport of xenobiotic conjugates in plants and
the exudation of a glutathione conjugate from the root tips. This could mean that plants possess an excretion system for unwanted
compounds which give them similar advantages as animals.
Materials and Methods:
Barley plants (Hordeum vulgare L. cv. Cherie) were grown in Petri dishes soaked with tap water in the greenhouse.
- Fluorescence Microscopy. Monobromo- and Monochlorobimane, two model xenobiotics that are conjugated rapidly in plant cells
with glutathione, hereby forming fluorescent metabolites, were used as markers for our experiments. Their transport in the
root could be followed sensitively with very good temporal and spatial resolution. Roots of barley seedlings were cut under
water and the end at which xenobiotics were applied was fixed in an aperture with a thin latex foil and transferred into a
drop of water on a cover slide. The cover slide was fixed in a measuring chamber on the stage of an inverse fluorescence microscope
(Zeiss Axiovert 100).
- Spectrometric enzyme assay. Glutathione S-transferase (GST) activity was determined in the protein extracts following established
methods. Aliquots of the enzyme extract were incubated with 1-chloro-2,4-dinitrobenzene (CDNB), or monochlorobimane. Controls
lacking enzyme or GSH were measured.
- Pitman chamber experiments. Ten days old barley plants or detached roots were inserted into special incubation chambers, either
complete with tips or decapitated, as well as 10 days old barley plants without root tips. Compartment A was filled with a
transport medium and GSH conjugate or L-cysteine conjugate. Compartments B and C contained sugar free media. Samples were
taken from the root tip containing compartment C and the amount of conjugate transported was determined spectro-photometrically.
Results:
The transport in roots is unidirectional towards the root tips and leads to exsudation of the conjugates at rates between
20 and 200 nmol min-1. The microscopic studies have been complemented by transport studies in small root chambers and spectroscopic
quantification of dinitrobenzene-conjugates. The latter experiments confirm the microscopic studies. Furthermore it was shown
that glutathione conjugates are transported at higher rates than cysteine conjugates, despite of their higher molecular weights.
This observation points to the existence of glutathione specific carriers and a specific role of glutathione in the root.
Discussion:
It can be assumed that long distance transport of glutathione conjugates within the plant proceeds like GSH or amino acid
transport in both, phloem and xylem. The high velocity of this translocation of the GS-X is indicative of an active transport.
For free glutathione, a rapid transport-system is essential because an accumulation of GSH in the root tip inhibits further
uptake of sulfur. Taking into account that all described MRP transporters and also the GSH plasmalemma ATPases have side activities
for glutathione derivatives and conjugates, co-transport of these xenobiotic metabolites seems credible.
- On the other hand, when GS-B was applied to the root tips from the outside, no significant uptake was observed. Thus it can
be concluded that only those conjugates can be transported in the xylem which are formed inside the root apex. Having left
the root once, there seems to be no return into the root vessels, probably because of a lack of inward directed transporters.
Conclusions:
Plants seem to possess the capability to store glutathione conjugates in the vacuole, but under certain conditions, these
metabolites might also undergo long range transport, predominantly into the plant root. The transport seems dependent on specific
carriers and is unidirectional, this means that xenobiotic conjugates from the rhizosphere are not taken up again. The exudation
of xenobiotic metabolites offers an opportunity to avoid the accumulation of such compounds in the plant.
Recommendations and Perspectives:
The role of glutathione and glutathione related metabolites in the rhizosphere has not been studied in any detail, and only
scattered data are available on interactions between the plant root and rhizosphere bacteria that encounter such conjugates.
The final fate of these compounds in the root zone has also not been addressed so far. It will be interesting to study effects
of the exuded metabolites on the biology of rhizosphere bacteria and fungi. 相似文献
946.
GOAL, SCOPE AND BACKGROUND: Pentachlorophenol (PCP) is the second highest volume pesticide used in the United States. It is a mutagenic compound whose exposure poses significant health effects, One of the most desirable, environmentally friendly treatment methods is bioremediation. For soil-based contamination, the effectiveness of bioremediation will also be affected by the presence of an active indigenous population, sorption of the contaminant onto the soil, and environmental parameters. METHODS: Two pure strains and their mixed culture were used to evaluate PCP biodegradation in two different field soils, Columbia (CO) and New Mexico (NM). Biostimulation of the indigenous microbes was evaluated by adding nutrients. The efficiency of adding bacteria strains (bioaugmentation) for degrading PCP was determined with Arthrobacter sp., Flavobacterium sp. and a 50:50 mixture of the two bacteria strains. RESULTS: In CO soil, only 24%, 12% and 25% of the initial PCP concentration were degraded by Flavobacterium sp., Arthrobacter sp. and mixed culture, respectively. Arthrobacter sp. was used in NM soil with two initial concentrations and achieved degradation efficiencies of 57% and 61% for 361 and 95 mg kg- concentrations, respectively. Discussion. Analysis via statistical methods showed that the bacteria had different efficiencies on PCP degradation in each soil. 2 CONCLUSIONS: All bacteria catalyzed a higher PCP degradation when present in NM soil. Second, Flavobacterium sp. degraded more PCP than Arthrobacter sp. in CO soil. The mixed culture achieved the highest degradation efficiency regardless of the initial concentration or soil origin. RECOMMENDATIONS AND PERSPECTIVES: The effect of the soil properties, such as the soil organic matter (SOM) on PCP biodegradation should be investigated. Future work can also investigate the effect of aging time on biodegradation. 相似文献
947.
Bioremediation process on Brazil shoreline 总被引:1,自引:0,他引:1
GOAL, SCOPE AND BACKGROUND: Bioremediation technique can be considered a promising alternative to clean oil spills using microbial processes to reduce the concentration and/or the toxicity of pollutants. To understand the importance of this work we must know that there is only little research performed to date using bioremediation techniques to clean oil spills in tropical countries. So, the main objective of this work is to analyze the behavior of a laboratory's bioremediation test using nutrients on coastal sediments. METHODS: The bioremediation process is followed through geochemical analysis during the tests. This organic material is analyzed by medium pressure liquid chromatography (MPLC), gas chromatography/flame ionization detection (GC/FID) and gas chromatography/ mass spectrometry. By microbial counting, the number of total bacteria and degrading bacteria is determined during the experiments, in order to confirm the effectiveness of the bioremediation process. The seawater obtained throughout the bioremediation process is analyzed for nutrients grade (phosphate and ammonium ions) and also for its toxicity (Microtox tests) due the presence of hydrocarbons and fertilizer. RESULTS: The results from the geochemical analyses of the oil show a relative decrease in the saturated hydrocarbon fraction that is compensated by a relative enrichment on polar compounds. It's confirmed by the fingerprint evaluation where it is possible to see a complete reduction of the normal alkanes followed by isoprenoids. Seawater analysis done by toxicity and nutrients analysis, such as microbial counting (total and degrading bacteria), confirm the fertilizer effectiveness during the bioremediation process. DISCUSSION: Results from simulating test using NPK, a low-price plant fertilizer, suggest that it's able to stimulate the degradation process. Results from medium pressure liquid chromatography (MPLC), done at two different depths (surface and subsurface), show different behavior during the biodegradation process where the later is seen to be more susceptible to microbial attack. Data from bioremediation unit shows a bigger reduction of the saturated fraction, followed by some smaller reduction of aromatic fractions, compensated by a relative increase from polar compounds (NSO). n-C17/pristane, n-C18/ fitane and pristane/fitane rates show constant values for the unity control, different from bioremediation samples which have a significant reduction, especially on subsurface areas, where a strong fall in the rates, seen to be reduced to zero over twenty days, had occurred during the first ten days. However, sample surfaces are reduced to zero in thirty days of experiments, proving that biodegradation is better on subsurfaces. Gaseous chromatography/mass spectrometry (CG/MS) analysis shows constant values to cyclic biomarker rates and aromatic compounds, suggesting that the biodegradation process is not strong enough to reduce these composites. Microbial analysis shows a reduction on heterotrophic (total bacteria) number from control unit, probably because the bacteria uses the spill oil like carbon source and energy. However, the number increases on bioremediation unit, because it uses NPK like a biostimulator. The hydrocarbonoclastic number isn't enough on the first moment, but it's detected after 30 days and quantified in all units, showing big values especially in bioremediation. Toxicity tests confirm that NPK fertilizer does not intoxicate the shoreline during the application of the bioremediation technique. Some nutrient concentration shows high values of ammonium and phosphate per bioremediation unit, reducing by the end of the experiment. CONCLUSIONS: Results reached the goal, finding a proper nutrient (NPK fertilizer) to stimulate the biodegradation process, growing bacteria responsible for reducing impact-contaminated coast ambient by oil spills. Chemical analysis of oil shows a reduction in the saturated fraction with a relative enrichment in polar composites (NSO) and the aromatic fraction from oil remaining constant. Subsurface samples show more biodegradation than surface samples, probably because the first one has higher humidity. Linear alcanes are more biodegraded than isoprenoids, confirming the biodegradation susceptibility order. Saturated cyclic biomarkers and aromatic compounds show constant behavior maybe because the nutrients or time was not enough for microorganismic attack. Fertilizer does not demonstrate any toxic effects in local biota so that it does not compromise the technique applicability and the environment is not saturated by nutrients during the simulation, especially since the coastal environment is an open system affected daily by tides. Therefore, bioremediation tests can be classified as moderate, reaching level 5 in the classification scale by Peters & Moldowan (1993). RECOMMENDATIONS AND PERSPECTIVES: The use of marine environment by the petroleum industry on exploration, production and transportation operation, transform this oil to become the most important pollutant in the oceans. Bioremediation is an important technique used to clean spilled oil impacting on shorelines, accelerating the biodegradation process by using fertilizer growing the microorganisms responsible for decontaminating the environment. We recommend confirming the efficiency of NPK nutrient used on bioremediation simulating experiments on beaches, while monitoring the chemical changes long-term. NPK fertilizer can be used to stimulate the biodegradation process on shoreline impacted by spilled oil. 相似文献
948.
Real-time measurement of outdoor tobacco smoke particles 总被引:1,自引:0,他引:1
Klepeis NE Ott WR Switzer P 《Journal of the Air & Waste Management Association (1995)》2007,57(5):522-534
The current lack of empirical data on outdoor tobacco smoke (OTS) levels impedes OTS exposure and risk assessments. We sought to measure peak and time-averaged OTS concentrations in common outdoor settings near smokers and to explore the determinants of time-varying OTS levels, including the effects of source proximity and wind. Using five types of real-time airborne particle monitoring devices, we obtained more than 8000 min worth of continuous monitoring data, during which there were measurable OTS levels. Measurement intervals ranged from 2 sec to 1 min for the different instruments. We monitored OTS levels during 15 on-site visits to 10 outdoor public places where active cigar and cigarette smokers were present, including parks, sidewalk cafés, and restaurant and pub patios. For three of the visits and during 4 additional days of monitoring outdoors and indoors at a private residence, we controlled smoking activity at precise distances from monitored positions. The overall average OTS respirable particle concentration for the surveys of public places during smoking was approximately 30 microg m(-3). OTS exhibited sharp spikes in particle mass concentration during smoking that sometimes exceeded 1000 microg m(-3) at distances within 0.5 m of the source. Some average concentrations over the duration of a cigarette and within 0.5 m exceeded 200 microg m(-3), with some average downwind levels exceeding 500 microg m(-3). OTS levels in a constant upwind direction from an active cigarette source were nearly zero. OTS levels also approached zero at distances greater than approximately 2 m from a single cigarette. During periods of active smoking, peak and average OTS levels near smokers rivaled indoor tobacco smoke concentrations. However, OTS levels dropped almost instantly after smoking activity ceased. Based on our results, it is possible for OTS to present a nuisance or hazard under certain conditions of wind and smoker proximity. 相似文献
949.
Damage to offshore oil and gas facilities following hurricanes Katrina and Rita: An overview 总被引:2,自引:0,他引:2
Hurricanes Katrina and Rita hit the centre of the American petrochemical industry, shutting down eight refineries, hundreds of oil-drilling and production platforms, and many other industrial facilities. Furthermore, it triggered unprecedented numbers of hazardous-materials releases from industrial facilities and storage terminals onshore, as well as from oil and gas production facilities offshore in the Gulf of Mexico (GoM). In this paper, we analyse the damage caused by the two hurricanes on the offshore oil and gas industry. Hurricanes Katrina and Rita caused the largest number of destroyed and damaged platforms and pipelines, and the highest number of mobile offshore drilling units set adrift in the history of GoM operations. Following the hurricanes, changes have been proposed to operating and emergency procedures, maintenance requirements, and design practices including mooring practices for mobile offshore drilling units. 相似文献
950.