Facilitation in an insect-pollinated herb with a floral display dimorphism

Population context should influence pollination success and selection on floral display in animal-pollinated plants because attraction of pollinators depends not only on the characteristics of individual plants, but also on the attractiveness of co-occurring conspecifics. The insect-pollinated herb Primula farinosa is polymorphic for inflorescence height. Natural populations may include both long-scaped plants, which present their flowers well above the soil surface, and short-scaped plants, with their flowers positioned close to the ground. We experimentally tested whether seed production in short-scaped P. farinosa varied with local morph frequency and surrounding vegetation height. In tall vegetation, short-scaped plants in polymorphic populations produced more fruit and tended to produce more seeds than short-scaped plants did in monomorphic populations. In low vegetation, population composition did not significantly affect fruit and seed output of short-scaped plants. The results suggest that long-scaped plants facilitate short-scaped plants in terms of pollinator attraction and that the facilitation effect is contingent on the height of the surrounding vegetation. The documented facilitation should contribute to the maintenance of the scape length polymorphism in ungrazed areas where litter accumulates and vegetation grows tall.     

Volatile organohalogen compounds in human urine: the effect of environmental exposure

The paper presents the results of determination of volatile organohalogen compounds (VOX) in urine samples from subjects exposed to these compounds in their workplaces and through consumption of chlorinated tap water. The analytes were isolated and preconcentrated from the complex urine samples using the thin layer headspace (TLHS) technique with autogenous generation of the liquid sorbent. Final gas chromatographic determination was carried out by direct aqueous injection with electron capture detection (DAI-ECD). The results indicate that only a small fraction (<4%) of the VOX input is excreted with urine in the non-metabolized form. A positive correlation was found between the occupational levels of VOX in the workplace and their levels in urine. VOX levels in the urine of subjects not exposed to them in the workplace were significantly lower. Their presence in the organisms was most probably related to consumption of tap water produced by chlorination of surface waters.     

Environmental assessment of different advanced oxidation processes applied to a bleaching Kraft mill effluent

Basic research on remediation of polluted sediment by leaching has, to date, been carried out exclusively with suspended material. For economic reasons, only solid-bed leaching is applicable to large-scale processes. Abiotic and microbial solid-bed leaching were comparatively studied in a percolator system using ripened and therefore permeable heavy metal polluted river sediment. In the case of abiotic leaching, sulfuric acid was supplied to the sediment by circulating water; the lower the pH of the percolating water, the higher the percolation flow, and the lower the solid-bed height was, the faster the heavy metals were solubilized. However, the pH and percolation flow are subjected to restrictions: strongly acidic conditions result in dissolution of mineral components, and the percolation flow must not exceed the bed permeability. And a high solid bed is an economic requirement. In the case of bioleaching, elemental sulfur added to the sediment was oxidized to sulfuric acid within the package which, in turn, solubilized the heavy metals. Here, the percolation flow and the solid-bed height did not affect the rate of metal solubilization. Solid-bed leaching on a larger scale will thus be much more efficient applying bioleaching with sulfur as the leaching agent than abiotic leaching with sulfuric acid.     

Presence of plutonium contamination in soils from Palomares (Spain)

More than 30 years after the occurrence of an aircraft accident which involved the detonation of two nuclear weapons in the surrounding area of the village of Palomares (Spain), the affected terrestrial area has been investigated for remaining transuranic contamination. Evidence from the presence of this contamination was initially found through the analysis of the 241Am inventories in superficial soil samples collected in the region, and was confirmed through the analysis of the (239+240)Pu inventories and their associated 238Pu/(239+240)Pu activity ratios in the same samples. However, it was also observed that a considerable fraction of the remaining contamination in the area was present in particulate form, i.e. as "hot particles". The work performed in our laboratory for identification, isolation and characterisation of these "hot particles" as well as some conclusions obtained from these analyses are outlined in this paper.     

Ecotoxicity, phytotoxicity and extractability of heavy metals from different stabilised sewage sludges

The presence of heavy metals in the sludges produced in wastewater treatment plants restricts their use for agricultural purposes. This study compares different types of sludges (aerobic, anaerobic, unstabilised and sludge from a waste stabilisation pond) in order to assess the extractability of heavy metals using simple extraction, water and DTPA. The stabilisation treatment undergone by the sludges influenced the heavy metals extractability. The least mineralised sludges (unstabilised and aerobic) showed higher metal extractability. The sewage sludges were subjected to chemical characterisation and toxicity testing (ecotoxicity and phytotoxicity assays) in absence of substrate, to provide a preliminary assessment of their suitability for land application. The ecotoxicity assays confirmed that no sludge constituted a hazardous waste. The sludge extracts had significant adverse effect on the germination index (GI) of barley (Hordeum vulgare L.) and cress (Lepidium sativum L.), a fact which indicates that some characteristics affected root growth.     

Presence of surfactants and their degradation intermediates in sediment cores and grabs from the Cadiz Bay area

The occurrence and distribution of the major surfactants--LAS, AES, APEOs and AEOs--and their degradation intermediates--SPCs, AP and APECs--in a marine-estuarine environment at Spain are presented. Results show that their concentration in surface sediments is clearly correlated with their usage and the existence of wastewater discharges. The degradation processes appear to lead to the formation of SPCs in the case of LAS, and to the shortening of the average ethoxylated chain length in the case of NPEOs, AEOs and AES. Vertical profiles for AEOs and AES are reported for the first time and present the highest values nearest the surface, followed by a sharp decrease with depth for all surfactants, as well as the appearance of degradation intermediates in deeper sedimentary layers. Shorter LAS homologues and SPCs tend to be present in pore water while strongly non-polar intermediates like NP are firmly attached to the sediments.     

Effect of acid rain on building material of the El Tajín archaeological zone in Veracruz, Mexico

Three limestone slabs (approx. 20 cm2 each) were extracted from the El Tajin archaeological zone in Veracruz, Mexico. X-ray diffraction analysis revealed three components: calcite (81.2%), quartz (17.9%) and feldspar (0.9%). Calcite content by x-ray diffraction analysis was slightly higher than that determined by chemical reaction between the limestone sample and nitric acid. The latter analysis, carried out in triplicate, yielded a calcite content of 77.1%. Mean water absorption, density and porosity of the limestone samples were also determined. Dissolution of limestone samples was investigated using an experimental rainfall simulation chamber, in which the stone samples were irrigated with artificial rain matching the pH and the ionic composition with 40 rainfall samples collected at El Tajin from August 18, 2002, to April 9, 2003. According to calcium and bicarbonate net concentrations found in the effluent of the chamber, a chemical mechanism by which limestone at El Tajin is dissolved by acid rain is proposed. A model used to investigate the air transport pathways corresponding to precipitation events at El Tajin shows that air parcels come mainly from the Gulf of Mexico, although no directional preference is evident for acidic vs. non-acidic events.     

Phytoavailability and fractions of iron and manganese in calcareous soil amended with composted urban wastes

Little is known about the effects of applying composted urban wastes on the phytoavailability and distribution of iron (Fe) and manganese (Mn) among chemical fractions in soil. In order to study this concern several experiments in pots containing calcareous soil were carried out. The received treatments by adding separately two rates (20 and 80 Mg ha-1) of municipal solid waste (MSW) compost and/or municipal solid waste and sewage sludge (MSW-SS) co-compost. The cropping sequence was a lettuce crop followed by a barley crop. It was observed that treatments amended with composted urban wastes tended to promote slight increases in lettuce yield compared to the control. The highest Fe levels in lettuce were found when higher rates of MSW-SS co-compost were applied; these values were significant compared to those obtained in the other treatments. In all cases, the application of organic materials increased the concentration and uptake of Mn in lettuce compared to the control; however, these increases were significant only when higher rates of MSW compost were applied. The organic amendments had beneficial delayed effects on barley yields, showing, in most cases, significant increases compared to the control. In this context, treatments with MSW compost were found to be more effective than the equivalent treatments amended with MSW-SS co-compost. Compared to the control, composted urban wastes increased Fe concentration in straw and rachis, and decreased Fe concentration in barley grain. Similarly, a decreased concentration of Mn in the dry matter of barley crop grown in soils treated with composted urban wastes was observed.     

Is levoglucosan a suitable quantitative tracer for wood burning? Comparison with receptor modeling on trace elements in Lycksele, Sweden

Particle emissions from residential wood combustion in small communities in Northern Sweden can sometimes increase the ambient particle concentrations to levels comparable to densely trafficked streets in the center of large cities. The reason for this is the combination of increased need for domestic heating during periods of low temperatures, leading to higher emission rates, and stable meteorological conditions. In this work, the authors compare two different approaches to quantify the wood combustion contribution to fine particles in Northern Sweden: a multivariate source-receptor analysis on inorganic compounds followed by multiple linear regression (MLR) of fine particle concentrations and levoglucosan used as a tracer. From the receptor model, it can be seen that residential wood combustion corresponds with 70% of modeled particle mass. Smaller contributions are also seen from local nonexhaust traffic particles, road dust, and brake wear (each contributing 14%). Of the mass, 1.5% is explained by long-distance transported particles, and 2% derives from a regional source deriving from either oil combustion or smelter activities. In samples collected in ambient air, a significant linear correlation was found between wood burning particles and levoglucosan. The levoglucosan fraction in the ambient fine particulate matter attributed to wood burning according to the multivariate analysis ranged from < 2% to 50%. This is much higher than the fraction found in the emission from the boilers expected to be responsible for most emissions at this site (between 3% and 6%). A laboratory emission study of wood and pellet boilers gave 0.3% wt to 22% wt levoglucosan to particle mass, indicating that the levoglucosan fraction may be highly dependent on combustion conditions, making it uncertain to use it as a quantitative tracer under real-world burning conditions. Thus, quantitative estimates of wood burning contributions will be very uncertain using solely levoglucosan as a tracer.