Rapid molecular prenatal diagnosis of ataxia-telangiectasia by direct mutational analysis

Mutations of the ataxia-telangiectasia-mutated (ATM) gene are responsible for the autosomal recessive disorder ataxia-telangiectasia (A-T). This study reports the first A-T prenatal diagnosis performed in Spain by direct molecular analysis. The pregnant woman had a previous child suffering from A-T due to a deletion in the ATM gene. The ATM coding region was sequenced in the A-T patient and her parents. Then, a specific polymerase chain reaction (PCR) to detect the deletion was performed for prenatal diagnosis. Additionally, polymorphic HLA loci were examined in order to exclude the possible contamination by maternal DNA. In this family of Gypsy origin, we carried out a rapid molecular diagnosis of A-T. Then, a prenatal diagnosis was carried out, identifying the deletion in the fetal DNA. Additionally, we performed a population study in unrelated Spanish Gypsies and in unrelated controls, showing that the deletion described could be a hotspot in the Spanish Gypsy population. The size of the coding region and the genomic structure, together with the absence of hotspots, make the mutation screening of the ATM gene difficult. The ability to identify ATM mutations provides a tool that can be applied in confirmatory diagnosis, genetic counselling, carrier prediction and prenatal diagnosis. Copyright © 2007 John Wiley & Sons, Ltd.     

Effect of combined nitrogen on dinitrogen fixation and productivity in Pisum sativum L. Inoculated with different strains of Rhizobium

This growth chamber study was designed to determine rates of nitrogen fixation, growth, productivity and concentrations of nitrogen and phosphorus during vegetative growth, flowering and the beginning of the fruiting and ripening period in Pisum sativum plants supplied with different concentrations of nitrate and nodulated by various strains of Rhizobium leguminosarum. Noninoculated control plants were grown under the same nutritional conditions. Nodulated and unnodulated plants responded differently to treatment. The effects of inoculation with Rhizobium and the nitrate treatments on the parameters of growth and nitrogen content varied with plant age. Although the presence of nitrate in the culture medium inhibited nodulation and acetylene reducing activity (ARA), the different Rhizobium strains used in the inoculations led to variations in tolerance to combined nitrogen.     

Transport of brine shrimps via the digestive system of migratory waders: dispersal probabilities depend on diet and season

Waterbirds are known to disperse invertebrate propagules that survive gut passage, but there is very little information about how the probability of dispersal changes at different times of the annual cycle when birds move in different directions, or how it is affected by changes in diet. We studied internal transport of brine shrimp Artemia cysts by migratory waders in the Odiel saltworks in south-west Spain. Viable cysts of parthenogenetic Artemia were abundant in the faeces and regurgitated pellets of redshank Tringa totanus, pellets of spotted redshank T. erythropus, and faeces of black-tailed godwit Limosa limosa during spring and/or autumn migrations in 2001–2002, but were not recorded during winter. Godwits did not produce pellets, and spotted redshank faeces were not sampled. Significant correlations between the number of cysts in a pellet or faecal sample and the proportion of that sample constituted by Artemia adults suggested that most cysts were ingested while in the ovisacs of gravid females. The proportion of cysts destroyed during digestion increased when accompanied by harder food items or grit, and when fewer cysts were ingested. The median number of intact cysts was higher in redshank faeces than in their pellets, but cysts extracted from pellets were more likely to hatch. A higher proportion of redshank pellets contained Artemia cysts in spring than in autumn, but more redshank migrated through the area in autumn. Significantly fewer cysts were recorded in redshank pellets in winter than in spring or autumn. Our results confirm that there is potential for long-distance dispersal of Artemia cysts via waders during both northwards (spring) and southwards (autumn) migrations.     

How Well Does Brazil's Environmental Law Work in Practice? Environmental Impact Assessment and the Case of the Itapiranga Private Sustainable Logging Plan

/ The Itapiranga Sustainable Logging Plan provides an example of how Brazil's licensing system functions for logging companies in the state of Amazonas. Two questions need to be dealt with: "How sustainable can logging in the Amazon be?" and "What and how effective are existing legal mechanisms to deal with logging projects?" The environmental impact assessment (EIA) and environmental impact statement (EIS, known as the RIMA in Brazil), present relatively detailed accounts of biodiversity and the need to adopt conservation strategies to protect it. However, social and health impacts are only superficially addressed. The economic sustainability of the operation over multiple cycles is not demonstrated. The multidisciplinary teams responsible for the EIA and EIS (RIMA) reports are hired by the project proponent, an arrangement inherently carrying the risk of biasing the result. Logging reduces biodiversity, releases greenhouse gases and inflicts social and health costs. These impacts reduce the ability of Amazonian forests to provide environmental services and to supply food and livelihood security to local populations. The reports inflate positive effects such as employment: the estimated number of jobs was cut by more than half in a revision made after the EIA and EIS (RIMA) had been approved. Not only do the reports need to be more realistic in assessing both positive and negative consequences of proposed projects, but better means are needed to ensure that promised mitigatory measures are enforced in practice. Many of the lessons that can be drawn from the Itapiranga Plan are not unique to logging projects and apply to licensing of development activites generally in Brazil and elsewhere.     

Strategies for management of Sicyos polyacanthus Cogn. (Cucurbitaceae) in sugarcane crops of Tucumán, Argentina

Sicyos polyacanthus is one of the most important weed in sugarcane crops of Tucumán (Argentina). The objective of this work was to establish strategies that would decrease the weed incidence in the crop to a minimum level. The study was carried out during 1998--2003 at five localities of sugarcane production of Tucumán (Argentina). The plots were 20 m long (192 m2) with 6 furrows and each plot was replicated five times. Treatments were: (i) Mechanical-chemical cultivation without fire; (ii) Mechanical-chemical culltivation with fire; (iii) Mechanical cultivation with handle pulled and with fire; (iv) Mechanical cultivation with handle pulled without fire; (v) Mechanical and chemical variants with fertilization and without fertilization; (vi) Mechanical and chemical variants with watering and without watering; (vii) Fallow and rotation, at the sugarcane crop renovation; (viii) Mechanical and chemical variants for plant cane and ratoon cane; and (ix) Mulching of harvest rests. The results suggest that besides the use of preemergent herbicides, fire marks the entry point of control, influencing fluxes and seed viability. It appears that fallow, mulching, and rotation of crops is fundamental for eliminating seeds that live short time in the soil and increase the mortality rate of species.     

Characteristic overpressure–impulse–distance curves for the detonation of explosives, pyrotechnics or unstable substances

A number of models have been proposed to calculate overpressure and impulse from accidental industrial explosions. When the blast is produced by explosives, pyrotechnics or unstable substances, the TNT equivalent model is widely used. From the curves given by this model, data are fitted to obtain equations showing the relationship between overpressure, impulse and distance. These equations, referred to here as characteristic curves, can be fitted by means of power equations, which depend on the TNT equivalent mass. Characteristic curves allow determination of overpressure and impulse at each distance.     

Carbon storage in Amazonia during the last glacial maximum: secondary data and uncertainties

The Amazonian forest is, due to its great size, carbon storage capacity and present-day variability in carbon uptake and release, an important component of the global carbon cycle. Paleo-environmental reconstruction is difficult for Amazonia due to the scarcity of primary palynological data and the mis-interpretation of some secondary data. Studies of lacustrine sediment records have shown that Amazonia has known periods in which the climate was drier than it is today. However, not all geomorphological features such as dunes, and slope erosion, which are thought to indicate rainforest regression, date from the time of the Late Glacial Maximum (LGM) and these features do not necessarily correspond to episodes of forest regression. There is also uncertainty concerning LGM carbon storage due to rainforest soils and biomass estimates. Soil carbon content may decrease moderately during the LGM, whereas rainforest biomass may change considerably in response to changes in the global environment. Biomass per unit area in Amazonia has probably been reduced by the cumulative effects of low CO2 concentration, a drier climate and lower temperatures. As few paleo-vegetation data are available, there is considerable uncertainty concerning the amount of carbon stored in Amazonia during the LGM, which may have corresponded to 44-94% of the carbon currently stored in biomass and soils.