Annual variation in the growth rate of Yoldia notabilis (Bivalvia: Nuculanidae) in Otsuchi Bay,northeastern Japan,analyzed using shell microgrowth patterns

Annual variation in the growth rate of the protobranch bivalve Yoldia notabilis (collected in Otsuchi Bay, northeastern Japan between 1989 and 1991) was determined by measuring shell length at successive growth lines. Factors affecting growth rates were assessed by examining long-term environmental data. Shell cross-sections of Y. notabilis showed a clear pattern of internal growth lines which formed simultaneously with the annual external lines on the outer shell surface. Hence, they were used as an age marker. A maximum lifespan of 17 yr was determined for this species, and a sigmoidal growth curve was obtained by the internal growth line analysis. Examination of the growth lines also revealed a large annual variation in growth rate, fluctuating as much as 32-fold during the past 9 yr. The variation correlated negatively with water temperature and positively with chlorophyll a content in the water column. It is suggested that the variation in annual growth rate is dependent on food supply during the spring phytoplankton bloom which varies from year to year according to the flow of the cold Oyashio current each spring.     

Inactivation of antineoplastics in clinical wastewater by electrolysis

Wastewater from clinical institutions contains a considerable amount of toxic substances. Among the toxic substances, antineoplastics may induce carcinogenesis, teratogenesis, and the emergence of mutant microorganisms in the environment. Although the incineration or chemical treatments of disposed antineoplastics are recommended, a high energy during incineration and a careful quality control during chemical treatment are required. In this study, we determined the conditions for the electrolytic treatment of an antineoplastic, epirubicin hydrochloride (EH), using two platinum electrodes with a constant current of 100 mA. We analyzed the cytotoxicity, mutagenicity and antibacterial activity of electrolyzed EH and compared them with those of unelectrolyzed EH. Nearly 100% cytotoxicity, mutagenicity and antibacterial activity were eliminated and HPLC did not detect an EH molecule, in the case of electrolysis for 6 h. We also examined the biological cytotoxicities of electrolyzed irinotecan hydrochloride, vincristine sulfate, mitomycin C, paclitaxel, methotrexate and cisplatin, and found that 72.1-99.999% toxicity was eliminated by electrolysis under the same conditions. The biological toxicity of a mixture of these drugs was determined to be decreased by approximately 99% by electrolysis under the same conditions.     

Development of a novel hybrid microwave-heater reactor for paper-based waste treatment

It is necessary to develop a medical waste management system featuring nonburning treatment and safety functions for small medical institutions. In this article, the development of a waste management system without oxygen injection was achieved by means of hybrid heating using microwave energy and an electric heater. The shape of the microwave reactor was a rectangular parallelepiped with a volume of about 0.1 m³. In the experimental setup, microwave energy (2.45 GHz, about 800 W) was injected from the top of the reactor, while the heater (about 1 kW) was located at the bottom. Heat insulators were set into all the walls of the reactor. The gases generated in the system were vented through water and activated carbon. Five paper-based diapers with absorbed water were used as the waste sample. For the evaluation of performance, the reduction rate was defined as the ratio (in percent) of the weight before and after treatment. The reduction rate as a function of treatment time and the effect of the position of the waste in the reactor on the reduction rate and the uniformity of treatment were examined for about 3 kg of waste. It was found that the reduction rate reached as low as 4.2% at 3 h and then 3% after 8 h. The treated profile strongly depended on the position of the waste in the reactor. In particular, it was clarified that a metal cylindrical enclosure and a needle electrode played an important role in attaining uniform treatment of the waste.     

Evaluation of electronic components in life cycle assessment

Life cycle assessment (LCA), a quantitative method for evaluating the total environmental impact of a product, from the materials in its manufacture to its final disposal, is playing an increasingly important role in manufacturing. When the LCA method is applied to a product containing many kinds of electronic components, there is a need for life cycle inventory (LCI) data on the components. This paper provides an original calculation of the LCI data for each electronic components industry. These data show the amount of input energy and emissions into the atmosphere per yen of production yield. It is demonstrated that the magnitude of the LCI data for each industry is essentially equal to that of the other industries. Furthermore, we conclude that the LCI data for all electronic components are roughly equivalent, making it possible to calculate the LCI data of any electronic component by simply multiplying the LCI data for the industry by the price of the component. Furthermore, after comparing the materials production stages with the component manufacturing stage in the calculation, it became clear that for several component industries the materials production stage could not be omitted from the calculation. Received: April 10, 1998 / Accepted: February 8, 1999     

Degradation of a Terephthalate-containing Polyester by Thermophilic Actinomycetes and <Emphasis Type="Italic">Bacillus</Emphasis> Species Derived from Composts

We intended to find thermophilic degraders of terephthalate-containing Biomax^® films. Films in mesh bags were buried in composts (inside temperature: approximately 55–60 °C), resulting in the degradation of them in 2 weeks. Fluorescent microscopy of films recovered from composts showed that microorganisms gradually covered the surface of a film during composting. DGGE analysis of microorganisms on the composted film indicated the presence of Bacillus species as main species (approximately 80% of microbial flora) and actinomycetes (approximately 10–20%) as the second major flora. Isolation of Biomax^®-utilizing bacteria was focused on these two genera: two actinomycetes and one Bacillus species were isolated as pure best degraders from the composted polymer films, which were fragmented into small pieces. All the strains were thermophilic and identified, based on their 16S rDNA analyses. Degradation of polymer films was confirmed by (1) accelerated fragmentation of films in composts, compared with a control (no inoculum) and resultant decrease in molecular weights, (2) growth in a powdered Biomax^® medium, compared with a control without powdered Biomax^®, and (3) production of terephthalate in a powdered Biomax^® medium. In this way, we concluded that these bacteria were useful for degradation of thermostable Biomax^® products.     

Environmental impact evaluation of feeds prepared from food residues using life cycle assessment

There is increasing concern about feeds prepared from food residues (FFR) from an environmental viewpoint; however, various forms of energy are consumed in the production of FFR. Environmental impacts of three scenarios were therefore investigated and compared using life cycle assessment (LCA): production of liquid FFR by sterilization with heat (LQ), production of dehydrated FFR by dehydration (DH), and disposal of food residues by incineration (IC). The functional unit was defined as 1 kg dry matter of produced feed standardized to a fixed energy content. The system boundaries included collection of food residues and production of feed from food residues. In IC, food residues are incinerated as waste, and thus the impacts of production and transportation of commercial concentrate feeds equivalent to the FFR in the other scenarios are included in the analysis. Our results suggested that the average amounts of greenhouse gas (GHG) emissions from LQ, DH, and IC were 268, 1073, and 1066 g of CO(2) equivalent, respectively. The amount of GHG emissions from LQ was remarkably small, indicating that LQ was effective for reducing the environmental impact of animal production. Although the average amount of GHG emissions from DH was nearly equal to that from IC, a large variation of GHG emissions was observed among the DH units. The energy consumption of the three scenarios followed a pattern similar to that of GHG emissions. The water consumption of the FFR-producing units was remarkably smaller than that of IC due to the large volumes of water consumed in forage crop production.     

EMF effects on microcirculatory system

Authors review the importance of studying the effects of electromagnetic fields (EMF) on microcirculatory system, especially in respect of possibility that vasculature may have direct and indirect role in interaction of static magnetic fields (SMF). We outline the physiological importance of microcirculation and relatively new methods of evaluation technique in vivo and explain in details the local and/or whole body exposure effects of SMF with␣range of 0.3–180 mT, power frequency EMF with range of 0.1–30 mT and microwaves at 1.5 GHz with range 0.08–8 W/kg brain average specific absorption rate (SAR) on microcirculatory systems in different tissues in experimental animals.