Effects of temperature on sorption-desorption processes of imidacloprid in soils of Croatian coastal regions

Sorption-desorption behavior of imidacloprid in six soils collected from five coastal regions in Croatia at 20, 30 and 40°C was investigated using batch equilibrium technique. Isothermal data were applied to Freundlich, Langmuir and Temkin equations, and the thermodynamic parameters ΔH°, ΔG°, ΔS° were calculated. The sorption isotherm curves were non-linear and may be classified as L-type, suggesting a relatively high sorption capacity for imidacloprid. Our results showed that the K( sor ) ( F ) values decreased for all the tested soils as the temperature increased, indicating that the temperature strongly influences the sorption. Values of ΔG° were negative (-4.65 to -2.00 kJ/mol) indicating that at all experimental temperatures the interactions of imidacloprid with soils were spontaneous processes. The negative and small ΔH° values (-19.79 to -8.89 kJ/mol) were in the range of weak forces, such as H-bonds, consistent with interactions and partitioning of the imidacloprid molecules into soil organic matter. The ΔS° values followed the range of -57.12 to -14.51 J/molK, suggesting that imidacloprid molecules lose entropy during transition from the solution phase to soil surface. It was found that imidacloprid desorption from soil was concentration and temperature-dependent, i.e. at lower imidacloprid concentrations and temperature, lower desorption percentage occurred. Desorption studies revealed that hysteretic behavior under different temperature treatments existed, and it was more pronounced at 20°C in the soils with higher OC content. The study results emphasize the importance of thermodynamic parameters in controlling soil pesticide mobility in different geographical locations, seasons and greenhouse conditions.     

Determination of the insecticide diflubenzuron in mushrooms by kinetic method and high-performance liquid chromatographic method

In the present study, a new sensitive and simple kinetic-spectrophotometric method for the determination of the insecticide diflubenzuron [1-(4-chlorophenyl)-3-(2,6-diflubenzoil)urea] is proposed. The method is based on the inhibited effect of diflubenzuron on the oxidation of sulphanilic acid (SA) by hydrogen peroxide in phosphate buffer in presence Cu(II) ion. Diflubenzuron was determined with linear calibration graph in the interval from 0.31 to 3.1 μg mL?1 and from 3.1 to 31.0 μg mL?1. The optimized conditions yielded a theoretical detection limit of 0.18 μg mL?1 corresponding to 0.036 mg kg(-1)mushroom sample based on the 3S(b) criterion. The RSD is 5.03-1.83 % and 2.81-0.71 % for the concentration interval of diflubenzuron 0.31-3.1 μg mL?1 and 3.1-31.0 μg mL?1, respectively. The reaction was followed spectrophotometrically at 370 nm. The kinetic parameters of the reaction are reported, and the rate equations are suggested. The developed procedure was successfully applied to the rapid determination of diflubenzuron in spiked mushroom samples of different mushroom species. The HPLC method was used like a comparative method to verify results.     

Malondialdehyde concentrations in the intestine and gills of Vardar chub (Squalius vardarensis Karaman) as indicator of lipid peroxidation

Environmental Science and Pollution Research - A lipid peroxidation product, malondialdehyde (MDA), was studied in Vardar chub (Squalius vardarensis Karaman) as an indicator of oxidative stress,...     

Exploring the Park–People Relation: Collection of <Emphasis Type="Italic">Vaccinium Myrtillus</Emphasis> L. by Local People From Kopaonik National Park in Serbia

Exploitation of certain resources within a protected area on a sustainable basis could contribute to higher living standards of rural people, particularly those in poor countries, and decrease conflicts between these populations and park authorities. This article presents data from a case study of Kopaonik National Park (NP), Serbia, which is a park with natural resources, most notably bilberries, which have always been relied on by local people. Vaccinium myrtillus traditionally has been collected for decades in Kopaonik NP and used by local people. However, little is known about the socioeconomic and ecological relations that affect the collection and use of this species. The aim of the present study was to understand how local people collect bilberries in Kopaonik NP and what their attitudes toward the park are. Household questionnaire data were used to examine how local people collect bilberries and how to improve the relations between local people and NP authorities. The survey questionnaire included 52 households from 7 local communities, and we applied a purposive sampling strategy. In addition, expert interviews were conducted, and from these data we obtained a broader understanding of the relation between local people and NP authorities. The results indicate that in Kopaonik NP, there is a conflict between pickers and NP authorities. Sustainable management should be directed toward the protection of bilberries. Education of local people is a key element in the sustainable collection and protection of natural resources.     

The Frequency of Micronuclei among Newborns from Kragujevac, Central Serbia, after NATO Bombing in the Spring of 1999

Kragujevac, as an important industrial and economic center of the region, is now placed on the top of the list of environmentally endangered cities in our country. The aim of this biomonitoring study was to evaluate cytogenetic damage in a sample of newborns from Kragujevac after contamination of the environment entailed by the intensive bombing of the industrial zone of this city in the spring of 1999. The frequency of micronuclei (MN) in peripheral blood lymphocytes in a total of 61 phenotypically healthy newborns was analyzed before and after the NATO bombing. Analysis of micronuclei has been performed using the cytokinesis-block technique (CB MN test). Average MN frequency in lymphocytes of newborns before the bombing (N = 25) was 5.77 ± 0.85/1000 analyzed cells. After the bombing (N = 36), the average frequency of MN increased by a factor of 1.4 (8.11 ± 0.85), compared to the control frequency before the bombing. Statistical difference (p < 0.05) was established by Student's t-test. Our data suggest that such changes in genetic material were a direct consequence of contamination of the living environment.     

Radiocesium accumulation in mosses from highlands of Serbia and Montenegro: chemical and physiological aspects

The aim of this work was (i) to determine the activity levels of 137Cs in mosses from highland ecosystems of Serbia and Montenegro, (ii) to find out if radiocesium is associated with essential biomacromolecules, and (iii) to investigate 137Cs distribution among intracellular compartments. It was found that biomolecules of mosses do not bind significant amounts of radiocesium (2.3-3.3% of the absorbed 137Cs), a behavior that was independent of the moss species. Cellular fractionation of mosses showed that membranes are the primary 137Cs-binding sites at the cellular level. They contained 26.1-43.1% of the initial radiocesium activity. It seems that 137Cs-binding molecules in different mosses are of similar chemical nature, and their distribution between various cellular compartments is not species specific.