Noninvasive prenatal diagnosis of aneuploidy using cell-free nucleic acids in maternal blood: promises and unanswered questions

The discovery of cell-free fetal (cff) DNA and RNA in the maternal circulation has driven developments in noninvasive prenatal diagnosis (NIPD) for the past decade. Detection of paternally derived alleles in cff DNA is becoming well established. Now much interest is focussing on NIPD of fetal chromosomal abnormalities, such as trisomy 21, which is a considerable challenge because this demands accurate quantitative measurements of the amounts of specific cff DNA or cff RNA sequences in maternal blood samples. Emerging strategies for distinguishing and quantifying the fetal nucleic acids in the maternal circulation promise continued development of the field, and pose a number of unanswered questions. Copyright © 2007 John Wiley & Sons, Ltd.     

Geochemical characterisation of the thermo-mineral waters of Greece

Environmental Geochemistry and Health - Geothermal areas of Greece are located in regions affected by recent volcanism and in continental basins characterised by elevated heat flow. Many of them...     

Exposure to polychlorinated biphenyls in residents near a chemical factory in Italy: the food chain as main source of contamination

High levels of PCBs were recently found in soil, food and some farmers living close to a chemical factory which until the 1980s had produced polychlorinated biphenyls (PCBs) in Brescia, North Italy. We performed a survey on a random sample of subjects aged 20-79 years living in various areas of the town with different levels of soil pollution to investigate factors associated with increased levels of PCB in serum (24 congeners were tested). Total PCB values were closely related to age (Spearman r=0.68; p<0.0001). The 166 consumers of locally produced food had higher PCB levels than non-consumers (median=1143 versus 719; 95th centile=9301 versus 2635ng/g lipid) with the highest levels among consumers of food produced in the most contaminated area close to the factory (median=2551; 95th centile=33464ng/g lipid). A dose-effect relationship between consumption of food produced in this area and PCB blood levels was observed (Spearman r=0.52, p=0.0014). Consumers of only plant food produced in this area had higher levels of PCB than non-consumers (median=1100; 95th centile=10,800ng/g lipid). Three subjects who had worked at the factory in the past showed high PCB levels. Distribution of PCB congeners did not differ between consumers of locally produced food and non-consumers, apart from PCB 209 which was found at high levels in former factory workers and was more common among consumers of food produced in the polluted area. In conclusion, we found high serum PCB levels in humans living in a highly polluted area in an industrialized town in Italy, due mainly to consumption of food produced in polluted areas.     

Climate impact potential of utilizing forest residues for bioenergy in Norway

The utilization of forest residues for bioenergy in Norway is foreseen to increase due to the government call to double bioenergy output by 2020 to thirty Tera-Watt hours. This study focuses on the climate impacts of bioenergy utilization where four forest residue extraction scenarios at clear-cut are considered: i) 75 % above ground residues (branches, (25 %) foliage, tops); ii) 75 % above and below ground residues (branches, tops, (25 %) foliage, stumps, coarse and small roots); iii) extracting 100 % of all available forest residue; and iv) leaving all residues in the forest. The Yasso07 soil-carbon model was utilized to quantify the carbon flux to the atmosphere due to the forest residues that are left in the forest in each scenario. The climate impact potential for each scenario was then calculated for the carbon-flux neutral Norway Spruce (Picea abies) forest system in five regions of Norway. The biogenic carbon dioxide emissions associated to decomposition upon forest floor, procurement losses and bioenergy conversion are included in these calculations. Results suggest that if such bioenergy can directly replace a fossil source of energy, the utilization of this biomass was found to be climatically beneficial in most fossil energy replacement cases and time horizons when compared to leaving the residues in the forest. Integrated global temperature change displacement factors have been developed which have been used to estimate the magnitude of this climate change mitigation over a particular time horizon.     

CORINE land cover and floristic variation in a Mediterranean wetland

The aims of the present study were to: (1) investigate whether CORINE land cover classes reflect significant differences in floristic composition, using a very detailed CORINE land cover map (scale 1:5000); (2) decompose the relationships between floristic assemblages and three groups of explanatory variables (CORINE land cover classes, environmental characteristics and spatial structure) into unique and interactive components. Stratified sampling was used to select a set of 100-m(2) plots in each land cover class identified in the semi-natural wetland surrounding a lake in central Italy. The following six classes were considered: stable meadows, deciduous oak dominated woods, hygrophilous broadleaf dominated woods, heaths and shrublands, inland swamps, canals or watercourses. The relationship between land cover classes and floristic composition was tested using several statistical techniques in order to determine whether the results remained consistent with different procedures. The variation partitioning approach was applied to identify the relative importance of three groups of explanatory variables in relation to floristic variation. The most important predictor was land cover, which explained 20.7% of the variation in plant distribution, although the hypothesis that each land cover class could be associated with a particular floristic pattern was not verified. Multi Response Permutation Analysis did not indicate a strong floristic separability between land cover classes and only 9.5% of species showed a significant indicator value for a specific land cover class. We suggest that land cover classes linked with hygrophilous and herbaceous communities in a wetland may have floristic patterns that vary with fine scale and are not compatible with a land cover map.     

Comparison of topological,empirical and optimization-based approaches for locating quality detection points in water distribution networks

Environmental Science and Pollution Research - The positioning of quality detection points as well as the frequency of sampling is a crucial aspect for the implementation of Water Safety Plans...     

Application of smart irrigation systems for water conservation in Italian farms

Environmental Science and Pollution Research - In many countries, water supplies are limited and must be managed for different uses. Providing additional resources for irrigation can be an...