Trace metal and stable isotope measurements (delta13C and delta15N) in the harbour porpoise Phocoena phocoena relicta from the Black Sea

Stable carbon and nitrogen isotopes (delta13C and delta15N) and trace metals (Cd, Zn, Cu, Fe, Se, and Hg) were analysed in the tissues of 46 harbour porpoises (Phocoena phocoena relicta) caught in fishing nets along the Ukrainian coasts between 1997 and 1998. Mean delta13C values differed significantly between male and female harbour porpoises suggesting a trophic segregation between sexes with a more coastal distribution for females at least during their gestation and nursing periods. Hepatic Hg was correlated to delta13C measurements, reflecting a different exposure linked to coastal vs offshore feeding habitats. A geographical comparison with existing data from other regions showed general low levels of Hg, Cd, Cu and Zn in the tissues of harbour porpoises from the Black Sea compared to other Atlantic and North Sea areas.     

Strong population differentiation of softshell clams (Mya arenaria) sampled across seven biogeographic marine ecoregions: possible selection and isolation by distance

Twenty-two Mya arenaria samples spanning seven marine ecoregions mostly situated in the Cold Temperate Northwest Atlantic (CTNA) biogeographic province were collected between 2001 and 2010 and genotyped at seven highly polymorphic microsatellite loci to test for population differentiation. Results showed strong regional differentiation with six genetic clusters: (1) Northern Gulf of St. Lawrence (GSL), (2) Magdalen Archipelago, (3) Southern GSL, (4) Lower Atlantic Canada, (5) US Coasts and (6) Northern Europe. Population structure was supported no matter the statistical approach and generally does not reflect the geographical limits of marine ecoregions. A latitudinal cline in allelic richness provides evidence for a northward post-glacial expansion range for this species. While geographical distance explains the genetic variation detected in southern CTNA, increased heterogeneity observed in northern CTNA can be explained by isolation by distance, marine landscaping and presumable selective processes acting at the Mar5 locus. Exclusion of Mar5 from analyses resulted in the detection of three genetic clusters instead of six.     

Effects of ocean acidification, temperature and nutrient regimes on the appendicularian Oikopleura dioica: a mesocosm study

Increasing pCO₂ is hypothesized to induce shifts in plankton communities toward smaller cells, reduced carbon export rates and increased roles of gelatinous zooplankton. Appendicularians, among the most numerous pan-global “gelatinous” zooplankton, continuously produce filter-feeding houses, shortcutting marine food webs by ingesting submicron particles, and their discarded houses contribute significantly to carbon fluxes. We present a first mesocosm-scale study on the effects of temperature, pCO₂ and bloom structures on the appendicularian, Oikopleura dioica. There were effects of temperature and nutrients on phytoplankton communities. No shifts in functional phytoplankton groups, nor changes in particle sizes/morphotypes, known to impact appendicularian feeding, were observed under manipulated pCO₂ conditions. However, appendicularian abundance was positively correlated with increased pCO₂, temperature and nutrient levels, consistent with hypotheses concerning gelatinous zooplankton in future oceans. This suggests appendicularians will play more important roles in marine pelagic communities and vertical carbon transport under projected ocean acidification and elevated temperature scenarios.     

Prenatal diagnosis of carnitine palmitoyltransferase 2 deficiency in chorionic villi: a novel approach

Carnitine palmitoyltransferase 2 (CPT2) deficiency, the most common autosomal recessive inherited disease of the mitochondrial long-chain fatty acid (LCFA) β-oxidation, may result in three distinct clinical phenotypes, namely, a mild adult muscular form, a severe infantile hepatocardiomuscular disease, and a neonatal form, which includes dysmorphic features in addition to hepatocardiomuscular symptoms. Both the latter forms are life-threatening diseases, and prenatal diagnosis (PND) can be offered to couples at a one-fourth risk of having an affected child. PND of CPT2 deficiency hitherto relied mostly on mutation detection from fresh chorionic villi (10 weeks' gestation), since CPT2 activity could be assayed on cultured amniocytes only (16–17 weeks' gestation). We devised a CPT2 activity assay from 10 mg of chorionic villi sampling (CVS). Combining this enzymatic assay to haplotype study using polymorphic markers linked to the CPT2 gene, we were able to carry out within 2 days, CPT2 deficiency PND, in two unrelated families, using a CVS performed at the 11th week of gestation. Copyright © 2003 John Wiley & Sons, Ltd.     

Prenatal diagnosis of exophytic nevus sebaceous of the scalp

Nevus sebaceous is a complex hamartoma most commonly found on the scalp, face, and neck and is often present at birth, although some may be diagnosed later in infancy. We report the first prenatal diagnosis of isolated nevus sebaceous that presented at 19 weeks' gestation as a large and exophytic tumor of the scalp. This case emphasizes the crucial role of ultrasound examination performed with high-frequency probes, which revealed associated diffuse lesions of the face. Identification of such a facial involvement could have a dramatic prognostic impact. © 2013 John Wiley & Sons, Ltd.     

Partial compensation of the sublethal effect of deltamethrin on the sex pheromonal communication of Trichogramma brassicae

Pyrethroid insecticides are widely used and lead to a sizable environmental pollution that could interfere with the population biology of insects. Trichogramma is a beneficial insect used in biological control and which natural populations contribute to the control of Lepidopterus pests. In this work, we determined the effect of a sublethal dose of deltamethrin on the sex pheromonal communication of Trichogramma. The dose used (LD 0.1) induces no detectable mortality (the theoretical mortality is only one insect over 1000) and can be a good representation of contamination by this insecticide from environmental pollution. The insecticide was shown to have opposite effects on the sex pheromonal communication of Trichogramma, depending on which sex was exposed (Delpuech, J.M., Legallet, B., Terrier, O., Fouillet, P., 1999. Chemosphere 38, 729–739). We show that, when both sexes are simultaneously exposed to the insecticide, this effect is only partially neutralized. The mean response of treated males responding to the sex pheromone from treated females is not significantly different from that of controls, but the kinetics of their response is not the same. When both sexes are treated, the response of males to the sex pheromone is lower at the beginning but their response does not decrease during time contrary to controls and becomes finally higher than that of controls. Therefore, the sublethal effect of deltamethrin in the field can be either advantageous or disadvantageous depending on the difficulty in finding females and their scarcity.